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The map label for this gene is estA [H]

Identifier: 50086591

GI number: 50086591

Start: 3565909

End: 3566979

Strand: Reverse

Name: estA [H]

Synonym: ACIAD3648

Alternate gene names: 50086591

Gene position: 3566979-3565909 (Counterclockwise)

Preceding gene: 50086592

Following gene: 50086590

Centisome position: 99.12

GC content: 40.52

Gene sequence:

>1071_bases
TTGACTGCATTTGTTCAAACCATTCAGGAAGTTTTAGAAAAAGGTCATGGGCCTGCTGCACGTGCTTTGGATAAGCTTCC
TTCCTTCGTACAAGAGTCTATTGCGAAAGTTTTAGGTTATCCCTATCAGTACCCGCAGTTAGATAGCTTTATCAAATGCT
TGATGGCAGTTCAAATCAAGCAAGGTCAAACTGGGTTTATCGGCTCAGATGTCGAAAAATCACGTTTAGCCTTTGAAACT
CAGATGGAGTCCATTCTTCGCAAGCCCACAGCCATCACGTTTGTCGAAGATATCCGCTTACCATTACAAAGCGGAACTAT
CTTTGCACGTCATTATCATCCTGCTCCAAACAAAAAATTACCTATGATTGTGTTCTATCATGGTGGAGGATTTGTGGTGG
GCAATGTTGATACACATGATGAAGCTTGTCGACTCATTGCAAAATACGCCAATGCTCAAGTATTAAGTATCGATTATCCA
TTGGCACCAGAAGTTTCACCGCAACGACTAATTCAGTCTTGTGAAGATGCCTTAGCTTGGGTTTATCAAAACAAACGTCA
TTTTAAAATACTAAAAAATCAGATTGCCGTGGCAGGTGATAGTGCGGGTGGAAATATCAGCACCGTTGTGGCTCAGCGTG
CGATTGGAAAAGTTTACGCACCAGATGCACAATTCCTGATTTATCCAGTAGTTGATTTTAAAAGCCGTCATCCCTCATTT
TATGCATATAAAGATGGATTGGTGCTGACTGGAAATGACGTCGACTATGTGACTGATTACTATGCAACAAAGCATGCTGT
ACATTTAGATGATCCAATTATTTCGCCTACCTATGGTAATTTCAAAAAACTGGCGCCTGCATATATTGTGACGGCTGGAC
ATGATGTATTACATGATGAAGGCGAAATCTATAGCCATAAGCTACGTCAAGCAGGGGTTAAAATTCATTTTGAAGAGTAC
CTTGATCAAACCCATGGATTTATCAATTTGACGCCAGTTTCACATAAGGCGAGAGCAAATCTGATTCAGATGAGTAAATC
ATTCCGTAAATTTTGGAATAAATACGCCTGA

Upstream 100 bases:

>100_bases
ATGTGACATCTTGATTGCGTACTTTAGACAACAATAGATTCAAGAACAGATAAATTGTATCGAGAAATCAAATGACAACG
ACGATGAAAGGAGAGAAGAA

Downstream 100 bases:

>100_bases
TATTAAGATCAGTTGAAATTTGATACTATTTGAAGAAATAGTATCAAATACACAATTAAAACAAGAGAGTAGACAGTTTA
TGCTATGTTAAGTCACTTAT

Product: carboxylesterase

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MTAFVQTIQEVLEKGHGPAARALDKLPSFVQESIAKVLGYPYQYPQLDSFIKCLMAVQIKQGQTGFIGSDVEKSRLAFET
QMESILRKPTAITFVEDIRLPLQSGTIFARHYHPAPNKKLPMIVFYHGGGFVVGNVDTHDEACRLIAKYANAQVLSIDYP
LAPEVSPQRLIQSCEDALAWVYQNKRHFKILKNQIAVAGDSAGGNISTVVAQRAIGKVYAPDAQFLIYPVVDFKSRHPSF
YAYKDGLVLTGNDVDYVTDYYATKHAVHLDDPIISPTYGNFKKLAPAYIVTAGHDVLHDEGEIYSHKLRQAGVKIHFEEY
LDQTHGFINLTPVSHKARANLIQMSKSFRKFWNKYA

Sequences:

>Translated_356_residues
MTAFVQTIQEVLEKGHGPAARALDKLPSFVQESIAKVLGYPYQYPQLDSFIKCLMAVQIKQGQTGFIGSDVEKSRLAFET
QMESILRKPTAITFVEDIRLPLQSGTIFARHYHPAPNKKLPMIVFYHGGGFVVGNVDTHDEACRLIAKYANAQVLSIDYP
LAPEVSPQRLIQSCEDALAWVYQNKRHFKILKNQIAVAGDSAGGNISTVVAQRAIGKVYAPDAQFLIYPVVDFKSRHPSF
YAYKDGLVLTGNDVDYVTDYYATKHAVHLDDPIISPTYGNFKKLAPAYIVTAGHDVLHDEGEIYSHKLRQAGVKIHFEEY
LDQTHGFINLTPVSHKARANLIQMSKSFRKFWNKYA
>Mature_355_residues
TAFVQTIQEVLEKGHGPAARALDKLPSFVQESIAKVLGYPYQYPQLDSFIKCLMAVQIKQGQTGFIGSDVEKSRLAFETQ
MESILRKPTAITFVEDIRLPLQSGTIFARHYHPAPNKKLPMIVFYHGGGFVVGNVDTHDEACRLIAKYANAQVLSIDYPL
APEVSPQRLIQSCEDALAWVYQNKRHFKILKNQIAVAGDSAGGNISTVVAQRAIGKVYAPDAQFLIYPVVDFKSRHPSFY
AYKDGLVLTGNDVDYVTDYYATKHAVHLDDPIISPTYGNFKKLAPAYIVTAGHDVLHDEGEIYSHKLRQAGVKIHFEEYL
DQTHGFINLTPVSHKARANLIQMSKSFRKFWNKYA

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI61966717, Length=290, Percent_Identity=24.1379310344828, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI21328446, Length=116, Percent_Identity=37.0689655172414, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI157041239, Length=192, Percent_Identity=27.0833333333333, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1786682, Length=261, Percent_Identity=28.3524904214559, Blast_Score=93, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI72001146, Length=143, Percent_Identity=38.4615384615385, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI71996133, Length=184, Percent_Identity=32.0652173913043, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17540028, Length=137, Percent_Identity=34.3065693430657, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24656084, Length=121, Percent_Identity=37.1900826446281, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24656076, Length=121, Percent_Identity=37.1900826446281, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI20130169, Length=121, Percent_Identity=37.1900826446281, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 39937; Mature: 39806

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAFVQTIQEVLEKGHGPAARALDKLPSFVQESIAKVLGYPYQYPQLDSFIKCLMAVQIK
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHC
QGQTGFIGSDVEKSRLAFETQMESILRKPTAITFVEDIRLPLQSGTIFARHYHPAPNKKL
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHCCCCCCCEEEEEEECCCCCCCC
PMIVFYHGGGFVVGNVDTHDEACRLIAKYANAQVLSIDYPLAPEVSPQRLIQSCEDALAW
CEEEEEECCCEEECCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHH
VYQNKRHFKILKNQIAVAGDSAGGNISTVVAQRAIGKVYAPDAQFLIYPVVDFKSRHPSF
HHCCCHHHHHHHHHHEEECCCCCCCHHHHHHHHHHCCEECCCCCEEEEEEEECCCCCCCE
YAYKDGLVLTGNDVDYVTDYYATKHAVHLDDPIISPTYGNFKKLAPAYIVTAGHDVLHDE
EEEECCEEEECCCCHHHHHHHHHCCEEECCCCCCCCCCCCHHHCCCEEEEECCCHHHCCC
GEIYSHKLRQAGVKIHFEEYLDQTHGFINLTPVSHKARANLIQMSKSFRKFWNKYA
CHHHHHHHHHCCCEEEHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TAFVQTIQEVLEKGHGPAARALDKLPSFVQESIAKVLGYPYQYPQLDSFIKCLMAVQIK
CHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHC
QGQTGFIGSDVEKSRLAFETQMESILRKPTAITFVEDIRLPLQSGTIFARHYHPAPNKKL
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHCCCCCCCEEEEEEECCCCCCCC
PMIVFYHGGGFVVGNVDTHDEACRLIAKYANAQVLSIDYPLAPEVSPQRLIQSCEDALAW
CEEEEEECCCEEECCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHH
VYQNKRHFKILKNQIAVAGDSAGGNISTVVAQRAIGKVYAPDAQFLIYPVVDFKSRHPSF
HHCCCHHHHHHHHHHEEECCCCCCCHHHHHHHHHHCCEECCCCCEEEEEEEECCCCCCCE
YAYKDGLVLTGNDVDYVTDYYATKHAVHLDDPIISPTYGNFKKLAPAYIVTAGHDVLHDE
EEEECCEEEECCCCHHHHHHHHHCCEEECCCCCCCCCCCCHHHCCCEEEEECCCHHHCCC
GEIYSHKLRQAGVKIHFEEYLDQTHGFINLTPVSHKARANLIQMSKSFRKFWNKYA
CHHHHHHHHHCCCEEEHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]