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The map label for this gene is rhdA [H]

Identifier: 50086515

GI number: 50086515

Start: 3486219

End: 3487076

Strand: Reverse

Name: rhdA [H]

Synonym: ACIAD3561

Alternate gene names: 50086515

Gene position: 3487076-3486219 (Counterclockwise)

Preceding gene: 50086516

Following gene: 50086514

Centisome position: 96.9

GC content: 47.2

Gene sequence:

>858_bases
TTGGACATCATGGCGGGATCTGAAATTAATCTTGGACTACTCATCGAAGCAGAAGAGCTGGTTCCGTATCTGGATCATGA
ATTACTACGGGTCGTAGATTTAAGCCGAAATTCTGTATATGAACAATTGCATCTGCCACAGGCGATTGCGGTTCGGCCTG
CACAATTAGTTCGCCAAGATGAGCTGGCGATGGGGTTACTGCCTGATGAAAAACAATTACAAGCACTGATTGAATATTTG
AATATCTCACCTCAGCATCATGTGGTGGTTTATGACGATGAGGGCGGAGCCTGGGCGGGACGTTTAATCTGGAATCTACA
TTGTCTGGGATTTACTCGCACGAGTTTGATCAATGGCGGGATTCATGCTTGGTTGGCCGCAGGCTTACCGACGACTTCAG
AAGCAGTGGTTCATGAGCCTGTATCTGATTTGCTCAAGGTCGATTTAAGCCATGTGGCTGATTATCAGATCGGTTATCCA
GCGCTTTTATCGCTGGTTCAGCAGCAAGCAATTCAATTGTGGGATTGTCGTACACTGGACGAATATACAGGCCTGCGTCT
AGCAGCGCGACGCGGCGGTCACATGCCAGGCGCACTTCATTTTGAGTGGAGTACTGCCCTTAATCGTGAAAATCATCTAA
AATTACATTCTTTTGAGCGCATCCAGCAGCGTTTGACACAACTTGGCTTCCAATTTAATCAGCCTGTCGTGATTTATTGC
CAATCCCATCATCGCTCTGGACTGGCATATATTGTGGCAAGACTATTGGGATGGTCAGTTCGTGCATATGATGGCGCTTG
GAGTGAATGGGGCAATCGCCTCGATAGTCCTGTGATTACCGGAGAACAGCCATTTTGA

Upstream 100 bases:

>100_bases
GCCTAATCAATTTCTATTTTTCTGCTAAGGATGCATGAAAAATCAGAAAGAGAGTGAAAAGGCTTATGATAAAATCGTAG
GTGCTAAAAATTGAATGTGA

Downstream 100 bases:

>100_bases
GCTTTACATCACGATTAAAAAAAGACCTATTTATTAAAGCACAATCGCTCGTTCCACAGCATCAGCTCAGCCGGGTGGTC
GGTAAGGTTGCCGAAAGCGA

Product: thiosulfate sulfurtransferase

Products: NA

Alternate protein names: Rhodanese-like protein [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MDIMAGSEINLGLLIEAEELVPYLDHELLRVVDLSRNSVYEQLHLPQAIAVRPAQLVRQDELAMGLLPDEKQLQALIEYL
NISPQHHVVVYDDEGGAWAGRLIWNLHCLGFTRTSLINGGIHAWLAAGLPTTSEAVVHEPVSDLLKVDLSHVADYQIGYP
ALLSLVQQQAIQLWDCRTLDEYTGLRLAARRGGHMPGALHFEWSTALNRENHLKLHSFERIQQRLTQLGFQFNQPVVIYC
QSHHRSGLAYIVARLLGWSVRAYDGAWSEWGNRLDSPVITGEQPF

Sequences:

>Translated_285_residues
MDIMAGSEINLGLLIEAEELVPYLDHELLRVVDLSRNSVYEQLHLPQAIAVRPAQLVRQDELAMGLLPDEKQLQALIEYL
NISPQHHVVVYDDEGGAWAGRLIWNLHCLGFTRTSLINGGIHAWLAAGLPTTSEAVVHEPVSDLLKVDLSHVADYQIGYP
ALLSLVQQQAIQLWDCRTLDEYTGLRLAARRGGHMPGALHFEWSTALNRENHLKLHSFERIQQRLTQLGFQFNQPVVIYC
QSHHRSGLAYIVARLLGWSVRAYDGAWSEWGNRLDSPVITGEQPF
>Mature_285_residues
MDIMAGSEINLGLLIEAEELVPYLDHELLRVVDLSRNSVYEQLHLPQAIAVRPAQLVRQDELAMGLLPDEKQLQALIEYL
NISPQHHVVVYDDEGGAWAGRLIWNLHCLGFTRTSLINGGIHAWLAAGLPTTSEAVVHEPVSDLLKVDLSHVADYQIGYP
ALLSLVQQQAIQLWDCRTLDEYTGLRLAARRGGHMPGALHFEWSTALNRENHLKLHSFERIQQRLTQLGFQFNQPVVIYC
QSHHRSGLAYIVARLLGWSVRAYDGAWSEWGNRLDSPVITGEQPF

Specific function: May Be A Sulfotransferase Involved In The Formation Of Thiosulfate. The Rhodanese Activity Of Ssea Is Weak, Its Participation In Detoxification Of Cyanide May Be Small. May Be Involved In The Enhancement Of Serine-Sensitivity. [C]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI17402865, Length=220, Percent_Identity=30, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI61835204, Length=237, Percent_Identity=25.7383966244726, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI194473681, Length=237, Percent_Identity=25.7383966244726, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI194473668, Length=237, Percent_Identity=25.7383966244726, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI87082121, Length=279, Percent_Identity=25.4480286738351, Blast_Score=85, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI71997283, Length=260, Percent_Identity=23.0769230769231, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17561888, Length=265, Percent_Identity=21.5094339622642, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 32132; Mature: 32132

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00683 RHODANESE_2 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIMAGSEINLGLLIEAEELVPYLDHELLRVVDLSRNSVYEQLHLPQAIAVRPAQLVRQD
CCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHH
ELAMGLLPDEKQLQALIEYLNISPQHHVVVYDDEGGAWAGRLIWNLHCLGFTRTSLINGG
HHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCEEHHHHEEEEECCHHHHHHHCCC
IHAWLAAGLPTTSEAVVHEPVSDLLKVDLSHVADYQIGYPALLSLVQQQAIQLWDCRTLD
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHH
EYTGLRLAARRGGHMPGALHFEWSTALNRENHLKLHSFERIQQRLTQLGFQFNQPVVIYC
HHHCHHHHHHCCCCCCCEEEEEHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEE
QSHHRSGLAYIVARLLGWSVRAYDGAWSEWGNRLDSPVITGEQPF
CCCCCCCHHHHHHHHHCCCCEECCCCHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MDIMAGSEINLGLLIEAEELVPYLDHELLRVVDLSRNSVYEQLHLPQAIAVRPAQLVRQD
CCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHH
ELAMGLLPDEKQLQALIEYLNISPQHHVVVYDDEGGAWAGRLIWNLHCLGFTRTSLINGG
HHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCEEHHHHEEEEECCHHHHHHHCCC
IHAWLAAGLPTTSEAVVHEPVSDLLKVDLSHVADYQIGYPALLSLVQQQAIQLWDCRTLD
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHH
EYTGLRLAARRGGHMPGALHFEWSTALNRENHLKLHSFERIQQRLTQLGFQFNQPVVIYC
HHHCHHHHHHCCCCCCCEEEEEHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEE
QSHHRSGLAYIVARLLGWSVRAYDGAWSEWGNRLDSPVITGEQPF
CCCCCCCHHHHHHHHHCCCCEECCCCHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8617271; 10788330 [H]