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The map label for this gene is gshA [H]

Identifier: 50086503

GI number: 50086503

Start: 3473622

End: 3475214

Strand: Reverse

Name: gshA [H]

Synonym: ACIAD3549

Alternate gene names: 50086503

Gene position: 3475214-3473622 (Counterclockwise)

Preceding gene: 50086509

Following gene: 50086502

Centisome position: 96.57

GC content: 44.63

Gene sequence:

>1593_bases
TTGCGAGCATCTTCCATGAGTCAACCCAACCCGACCTCTCAATCTGTAGTGCAAACTTGGGTGGATTCGTCACATTTACA
AGGTATGCTGCGTGGCATCGAGCGTGAAAGCTTGCGTATGCAAGGCAATGGTTTTCTTTCACAGAAGCCTCATCCTGCGG
GATTGGGTTCAGCGTTAACCCACTCGCGTATTACCACAGACTATTCAGAAGCCTTGATGGAGTTCATCACACCGCCTAAA
GAAACGATTACTGAAGTTCTGGCGGAACTCACAGAGATTCATGCTGTGGTCCAGCGCCATCTCGATCAAGATGAGAAACT
TTGGCCATTGTCAATGCCGTGTATGCTGGATGATGAGGAAGAGCAAATTCCTTTGGCATACTATGGCACATCTAATATCG
GACGTTTTAAAACGCTCTATCGCCGTGGTTTGGGTGTCCGTTATGGACGCAGAATGCAGACGATTTCAGGTGTACATTAT
AATCTTTCTTTTCCTGATGCCTTATTTGAATTATTACAACAACACGAGTCTGATGACACGCTTAAACAACTAAGCCTGCA
AGATTATCGCAGTCATCGTTATTTTGGTCTGATTCGTAATTTTATTCGTCTAACGCCTTTGGTGATTTTTTTGGTCGGGG
CGAGTCCATCGGTATGTCGATGCTTCCTAACAGGACGTGAACATAATTTGCAGCCTCTGATCAAGGGAACCATGTACCTG
CCTGATGCAACTGCTTTACGTATGGGGCGTTTGGGATATCAGAACTCTGCCCAGAAAAAACTTGGCATTCATTACAATGA
TTTGCATGGTTACCTTGAAGGTTTACAAAATGCGGTTAAAACGTCTTACGCGCCATTTAGCGACTTGGGTTTAAACGATG
AACAAGGCCAACCGATTCAGATCAATGATCATATTTTACAAATTGAAAATGAATATTATAGTCTAGTCCGTCCAAAACAA
GTGCCTCAGCCTGGAGAAACACCCTCAGACGCGCTTAAAAATCGGGGTGTAGGTTACGTTGAGTTACGTGCTGTCGATGT
TAACCCCTATAGTCCGATTGGAATTGATGAAACTACGGCTGGTTTTTTAGAGGCCTTGGCCTTGTATTGCTTACTCACAG
AAAGCCCAGCTTTAGAAACAGATGAGCAGGATCGGATTGAGCGTAATCAGACTGAAGTCGTCAATCGCGGACGTGCTGTC
GACGCCCAGATTGAAACAGCTCAGGGTTTTATCTCCGTTCAGGAATGGGCAAAACAACATGTGTTGGCCATGCAGGAGTG
TGCAAACTTACTGGATCAATATTACCAGACCACGCTCTATCAAGATGCACTGGATGAGATGCAGCAACGAATTGATCAGA
GTAAATCCGCGTTGTCTGCACAAGTAGTACAGGACACACTCAAACACGGCGGTACATGGAGTTTTGGTAGTGTACTGGCA
GAGCAGCATGCGCAGACTTATACGCAGTATCCTTTAAGTGCTGAAAAACTGGCTTATTTTAAGCAGCTTGCACAGCAGTC
TTTACAACAACAGTATCAACTCGAACAAGAGTCTTCGGTGAGTTTTGAAGATTATCTTGCTCAATTTCGATAA

Upstream 100 bases:

>100_bases
AAAATATTCAATTTCATCAAAATCAGTTTTGGTGCTGCTATGTTAATCACAATTCAAGTTATACTAATAACATCATGATT
TTATGTAGTAGTTCTATCAT

Downstream 100 bases:

>100_bases
AGAATGAGGAATCACCATGCAATGGAGTTATCGCTTCGTAAGTGGTTTATTGGTTCTGGCCAGTATTGTGGGTATGACCT
TTGCGTTATATCTAGAACAT

Product: gamma-glutamate-cysteine ligase

Products: NA

Alternate protein names: Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase [H]

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MRASSMSQPNPTSQSVVQTWVDSSHLQGMLRGIERESLRMQGNGFLSQKPHPAGLGSALTHSRITTDYSEALMEFITPPK
ETITEVLAELTEIHAVVQRHLDQDEKLWPLSMPCMLDDEEEQIPLAYYGTSNIGRFKTLYRRGLGVRYGRRMQTISGVHY
NLSFPDALFELLQQHESDDTLKQLSLQDYRSHRYFGLIRNFIRLTPLVIFLVGASPSVCRCFLTGREHNLQPLIKGTMYL
PDATALRMGRLGYQNSAQKKLGIHYNDLHGYLEGLQNAVKTSYAPFSDLGLNDEQGQPIQINDHILQIENEYYSLVRPKQ
VPQPGETPSDALKNRGVGYVELRAVDVNPYSPIGIDETTAGFLEALALYCLLTESPALETDEQDRIERNQTEVVNRGRAV
DAQIETAQGFISVQEWAKQHVLAMQECANLLDQYYQTTLYQDALDEMQQRIDQSKSALSAQVVQDTLKHGGTWSFGSVLA
EQHAQTYTQYPLSAEKLAYFKQLAQQSLQQQYQLEQESSVSFEDYLAQFR

Sequences:

>Translated_530_residues
MRASSMSQPNPTSQSVVQTWVDSSHLQGMLRGIERESLRMQGNGFLSQKPHPAGLGSALTHSRITTDYSEALMEFITPPK
ETITEVLAELTEIHAVVQRHLDQDEKLWPLSMPCMLDDEEEQIPLAYYGTSNIGRFKTLYRRGLGVRYGRRMQTISGVHY
NLSFPDALFELLQQHESDDTLKQLSLQDYRSHRYFGLIRNFIRLTPLVIFLVGASPSVCRCFLTGREHNLQPLIKGTMYL
PDATALRMGRLGYQNSAQKKLGIHYNDLHGYLEGLQNAVKTSYAPFSDLGLNDEQGQPIQINDHILQIENEYYSLVRPKQ
VPQPGETPSDALKNRGVGYVELRAVDVNPYSPIGIDETTAGFLEALALYCLLTESPALETDEQDRIERNQTEVVNRGRAV
DAQIETAQGFISVQEWAKQHVLAMQECANLLDQYYQTTLYQDALDEMQQRIDQSKSALSAQVVQDTLKHGGTWSFGSVLA
EQHAQTYTQYPLSAEKLAYFKQLAQQSLQQQYQLEQESSVSFEDYLAQFR
>Mature_530_residues
MRASSMSQPNPTSQSVVQTWVDSSHLQGMLRGIERESLRMQGNGFLSQKPHPAGLGSALTHSRITTDYSEALMEFITPPK
ETITEVLAELTEIHAVVQRHLDQDEKLWPLSMPCMLDDEEEQIPLAYYGTSNIGRFKTLYRRGLGVRYGRRMQTISGVHY
NLSFPDALFELLQQHESDDTLKQLSLQDYRSHRYFGLIRNFIRLTPLVIFLVGASPSVCRCFLTGREHNLQPLIKGTMYL
PDATALRMGRLGYQNSAQKKLGIHYNDLHGYLEGLQNAVKTSYAPFSDLGLNDEQGQPIQINDHILQIENEYYSLVRPKQ
VPQPGETPSDALKNRGVGYVELRAVDVNPYSPIGIDETTAGFLEALALYCLLTESPALETDEQDRIERNQTEVVNRGRAV
DAQIETAQGFISVQEWAKQHVLAMQECANLLDQYYQTTLYQDALDEMQQRIDQSKSALSAQVVQDTLKHGGTWSFGSVLA
EQHAQTYTQYPLSAEKLAYFKQLAQQSLQQQYQLEQESSVSFEDYLAQFR

Specific function: Glutathione biosynthesis; first step. [C]

COG id: COG2918

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789044, Length=512, Percent_Identity=39.2578125, Blast_Score=350, Evalue=2e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007370
- InterPro:   IPR006334 [H]

Pfam domain/function: PF04262 Glu_cys_ligase [H]

EC number: =6.3.2.2 [H]

Molecular weight: Translated: 60087; Mature: 60087

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRASSMSQPNPTSQSVVQTWVDSSHLQGMLRGIERESLRMQGNGFLSQKPHPAGLGSALT
CCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHH
HSRITTDYSEALMEFITPPKETITEVLAELTEIHAVVQRHLDQDEKLWPLSMPCMLDDEE
HHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
EQIPLAYYGTSNIGRFKTLYRRGLGVRYGRRMQTISGVHYNLSFPDALFELLQQHESDDT
CCCCEEEECCCCCHHHHHHHHHCCCCHHCCHHHHHCCCEEECCCHHHHHHHHHHCCCCHH
LKQLSLQDYRSHRYFGLIRNFIRLTPLVIFLVGASPSVCRCFLTGREHNLQPLIKGTMYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHEECCCCCCCCHHHHCCEEC
PDATALRMGRLGYQNSAQKKLGIHYNDLHGYLEGLQNAVKTSYAPFSDLGLNDEQGQPIQ
CCHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCEE
INDHILQIENEYYSLVRPKQVPQPGETPSDALKNRGVGYVELRAVDVNPYSPIGIDETTA
ECCCEEEECHHHHHHCCCCCCCCCCCCHHHHHHCCCCCEEEEEEEECCCCCCCCCCHHHH
GFLEALALYCLLTESPALETDEQDRIERNQTEVVNRGRAVDAQIETAQGFISVQEWAKQH
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
VLAMQECANLLDQYYQTTLYQDALDEMQQRIDQSKSALSAQVVQDTLKHGGTWSFGSVLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
EQHAQTYTQYPLSAEKLAYFKQLAQQSLQQQYQLEQESSVSFEDYLAQFR
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCC
>Mature Secondary Structure
MRASSMSQPNPTSQSVVQTWVDSSHLQGMLRGIERESLRMQGNGFLSQKPHPAGLGSALT
CCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHH
HSRITTDYSEALMEFITPPKETITEVLAELTEIHAVVQRHLDQDEKLWPLSMPCMLDDEE
HHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
EQIPLAYYGTSNIGRFKTLYRRGLGVRYGRRMQTISGVHYNLSFPDALFELLQQHESDDT
CCCCEEEECCCCCHHHHHHHHHCCCCHHCCHHHHHCCCEEECCCHHHHHHHHHHCCCCHH
LKQLSLQDYRSHRYFGLIRNFIRLTPLVIFLVGASPSVCRCFLTGREHNLQPLIKGTMYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHEECCCCCCCCHHHHCCEEC
PDATALRMGRLGYQNSAQKKLGIHYNDLHGYLEGLQNAVKTSYAPFSDLGLNDEQGQPIQ
CCHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCEE
INDHILQIENEYYSLVRPKQVPQPGETPSDALKNRGVGYVELRAVDVNPYSPIGIDETTA
ECCCEEEECHHHHHHCCCCCCCCCCCCHHHHHHCCCCCEEEEEEEECCCCCCCCCCHHHH
GFLEALALYCLLTESPALETDEQDRIERNQTEVVNRGRAVDAQIETAQGFISVQEWAKQH
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
VLAMQECANLLDQYYQTTLYQDALDEMQQRIDQSKSALSAQVVQDTLKHGGTWSFGSVLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
EQHAQTYTQYPLSAEKLAYFKQLAQQSLQQQYQLEQESSVSFEDYLAQFR
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA