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The map label for this gene is hisF [H]

Identifier: 50086378

GI number: 50086378

Start: 3320641

End: 3321450

Strand: Direct

Name: hisF [H]

Synonym: ACIAD3404

Alternate gene names: 50086378

Gene position: 3320641-3321450 (Clockwise)

Preceding gene: 50086372

Following gene: 50086380

Centisome position: 92.28

GC content: 46.17

Gene sequence:

>810_bases
CTGCAATCTCGGAAAATTTATTTATTTAGACTCATACTGTTGGATGCCACAATGCTTGCAAAACGTATTATTCCCTGCCT
TGATGTCGATAATGGCCGTGTGGTCAAGGGCGTGCAATTTTTAGATATTCGTGATGCAGGTGATCCTGTTGAAGTTGCAC
GTCGCTACAATGAGCAAGGTGCAGATGAAATTACTTTTCTGGATATTACTGCAACGCATCATGGTCGTGATACCACCTAT
CGTACAGTTGAACGTATGGCAGAAAGCGTATTTGTCCCTCTGACTGTCGGGGGTGGTGTGCGTAAGGTGGAAGACATTCG
TTTATTGCTGAATGCAGGTGCAGATAAAGTGAGCATTAACTCGGCAGCCGTGTTTAATCCTGAGTTTGTACAAGAAGCAT
CACAACGCTTTGGGGCCCAATGTATTGTGGTTGCAATTGATGCTAAAAAAACAGGCGACAACACATGGGAAATTTTTACC
CACGGTGGTCGTAAGCCTACGGGCATTGATGCACTGGAGTGGTCGGTGAAGATGGCAGAATACGGCGCAGGCGAACTGCT
GGTGACCAGTATGGATGCCGATGGTACCAAAGCAGGTTATGACATTGCCTTGATGCGTGAAATTAACAACCGTGTTTCAA
TTCCCACCATTGCCTCTGGCGGCGTAGGGAATTTACAGCATATGGCAGATGGGATTTTAAAAGGTGGTGCAGATGCGGTA
CTGGCAGCCAGTATTTTCCACTTTGGTCAGCATACCATTCCTGAAGCAAAACAGTTTTTGGCTGCACAAGGCATCGAAAT
GCGTCTCTAA

Upstream 100 bases:

>100_bases
TGAACTTCCTGTAAACTCAACGGGGTATAAACCGACATATAAAACCTGCGTGAAACAAATATAAGTTTGATAGAATACCC
AAGCAGTTTACCAAGATTCT

Downstream 100 bases:

>100_bases
GGACTTAGTTTATACAATTTGTATGCCCTCACTGTAGACTCATTTCGATAGTGGATCGCGAGATAAATTTAAGCGATCCT
TTTTATTGATGATCAACAAA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MQSRKIYLFRLILLDATMLAKRIIPCLDVDNGRVVKGVQFLDIRDAGDPVEVARRYNEQGADEITFLDITATHHGRDTTY
RTVERMAESVFVPLTVGGGVRKVEDIRLLLNAGADKVSINSAAVFNPEFVQEASQRFGAQCIVVAIDAKKTGDNTWEIFT
HGGRKPTGIDALEWSVKMAEYGAGELLVTSMDADGTKAGYDIALMREINNRVSIPTIASGGVGNLQHMADGILKGGADAV
LAASIFHFGQHTIPEAKQFLAAQGIEMRL

Sequences:

>Translated_269_residues
MQSRKIYLFRLILLDATMLAKRIIPCLDVDNGRVVKGVQFLDIRDAGDPVEVARRYNEQGADEITFLDITATHHGRDTTY
RTVERMAESVFVPLTVGGGVRKVEDIRLLLNAGADKVSINSAAVFNPEFVQEASQRFGAQCIVVAIDAKKTGDNTWEIFT
HGGRKPTGIDALEWSVKMAEYGAGELLVTSMDADGTKAGYDIALMREINNRVSIPTIASGGVGNLQHMADGILKGGADAV
LAASIFHFGQHTIPEAKQFLAAQGIEMRL
>Mature_269_residues
MQSRKIYLFRLILLDATMLAKRIIPCLDVDNGRVVKGVQFLDIRDAGDPVEVARRYNEQGADEITFLDITATHHGRDTTY
RTVERMAESVFVPLTVGGGVRKVEDIRLLLNAGADKVSINSAAVFNPEFVQEASQRFGAQCIVVAIDAKKTGDNTWEIFT
HGGRKPTGIDALEWSVKMAEYGAGELLVTSMDADGTKAGYDIALMREINNRVSIPTIASGGVGNLQHMADGILKGGADAV
LAASIFHFGQHTIPEAKQFLAAQGIEMRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=42.4124513618677, Blast_Score=207, Evalue=5e-55,
Organism=Escherichia coli, GI87082028, Length=241, Percent_Identity=31.1203319502075, Blast_Score=99, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=33.4394904458599, Blast_Score=162, Evalue=6e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 29247; Mature: 29247

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSRKIYLFRLILLDATMLAKRIIPCLDVDNGRVVKGVQFLDIRDAGDPVEVARRYNEQG
CCCCEEEEHHHHHHHHHHHHHHCCCEEECCCCCEEECEEEEEECCCCCHHHHHHHHCCCC
ADEITFLDITATHHGRDTTYRTVERMAESVFVPLTVGGGVRKVEDIRLLLNAGADKVSIN
CCEEEEEEEEECCCCCCHHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHCCCCEEECC
SAAVFNPEFVQEASQRFGAQCIVVAIDAKKTGDNTWEIFTHGGRKPTGIDALEWSVKMAE
CEEEECHHHHHHHHHHCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCCHHHHEEEHHH
YGAGELLVTSMDADGTKAGYDIALMREINNRVSIPTIASGGVGNLQHMADGILKGGADAV
CCCCEEEEEEECCCCCCCCCCEEEEEHHCCCCCCCEECCCCCCCHHHHHHHHHCCCCHHH
LAASIFHFGQHTIPEAKQFLAAQGIEMRL
HHHHHHHHCCCCCHHHHHHHHHCCCEECC
>Mature Secondary Structure
MQSRKIYLFRLILLDATMLAKRIIPCLDVDNGRVVKGVQFLDIRDAGDPVEVARRYNEQG
CCCCEEEEHHHHHHHHHHHHHHCCCEEECCCCCEEECEEEEEECCCCCHHHHHHHHCCCC
ADEITFLDITATHHGRDTTYRTVERMAESVFVPLTVGGGVRKVEDIRLLLNAGADKVSIN
CCEEEEEEEEECCCCCCHHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHCCCCEEECC
SAAVFNPEFVQEASQRFGAQCIVVAIDAKKTGDNTWEIFTHGGRKPTGIDALEWSVKMAE
CEEEECHHHHHHHHHHCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCCHHHHEEEHHH
YGAGELLVTSMDADGTKAGYDIALMREINNRVSIPTIASGGVGNLQHMADGILKGGADAV
CCCCEEEEEEECCCCCCCCCCEEEEEHHCCCCCCCEECCCCCCCHHHHHHHHHCCCCHHH
LAASIFHFGQHTIPEAKQFLAAQGIEMRL
HHHHHHHHCCCCCHHHHHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA