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The map label for this gene is hisA

Identifier: 50086372

GI number: 50086372

Start: 3311544

End: 3312275

Strand: Direct

Name: hisA

Synonym: ACIAD3398

Alternate gene names: 50086372

Gene position: 3311544-3312275 (Clockwise)

Preceding gene: 50086371

Following gene: 50086378

Centisome position: 92.02

GC content: 46.72

Gene sequence:

>732_bases
ATGCTGATCATCCCTGCAATTGACCTGAAAGATGGTAAATGTGTCCGCCTCAAGCAAGGTCGTATGGAAGACGATACCGT
TTTCTCTGACGATCCAGTTGCAACCGCACAGCATTGGGTCAATGAAGGTGCACGTCGTTTACATTTGGTCGATTTGAATG
GTGCCTTCGCAGGAACCCCCATTCATAAACCAGTGGTTGAAGCAATTGCCAAAGCACAACCTGAGCTTCCCATCCAGATT
GGCGGTGGTATTCGCTCCCTTGAAACCATTGAGCATTATTTAGATGCAGGCGTATCCTTTGTCATTATTGGTACCAAAGC
CGTACAAGATCCTGAGTTTGTCGAGCAGGCATGCAAACAGTTTGCTGGTCATATCATTGTAGGCATCGATGCCAAAGATG
GCATGGTGGCAACCGATGGCTGGGCCAATGTGACAGATGTAAAAGCAACTGATTTGGCCAAACGTTTTGCCGATGCAGGT
GTTTCAAGCATTGTATATACCGATATTGCACGTGATGGCATGATGCAAGGTGTAAATGTCGAGCAGACTGTCAATCTGGC
GCAGTATTCTGGCTTACCCGTGATTGCTTCTGGCGGTGTTACCAATCTGGATGATGTACGCTTACTCAAAGGCAAGCCTG
GTATTTTAGGAGCCATTACTGGTCGTGCCATTTATGAAGGTACGCTCAGCCTTCGTGAAGCACAGTTATTGCTTGATCAA
AATACGCTTTAA

Upstream 100 bases:

>100_bases
ATTTCGGTCAAATCGGCGCGTTGTTTGCTTAGTGATTTTAAAATAGTTTGTTAAGATGAGACAATTTTTTTAGTTTAAAG
ATGAAGCAAGGAGCGAAAGC

Downstream 100 bases:

>100_bases
TACATTTAAGCTAAAAAAAAGATGCCTCACGAGGCATCTTTTTTTTATCTCTTATCGTTTATCCATTTGTCCATGGCTGG
GCTGGTGAACCAATCCATGC

Product: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MLIIPAIDLKDGKCVRLKQGRMEDDTVFSDDPVATAQHWVNEGARRLHLVDLNGAFAGTPIHKPVVEAIAKAQPELPIQI
GGGIRSLETIEHYLDAGVSFVIIGTKAVQDPEFVEQACKQFAGHIIVGIDAKDGMVATDGWANVTDVKATDLAKRFADAG
VSSIVYTDIARDGMMQGVNVEQTVNLAQYSGLPVIASGGVTNLDDVRLLKGKPGILGAITGRAIYEGTLSLREAQLLLDQ
NTL

Sequences:

>Translated_243_residues
MLIIPAIDLKDGKCVRLKQGRMEDDTVFSDDPVATAQHWVNEGARRLHLVDLNGAFAGTPIHKPVVEAIAKAQPELPIQI
GGGIRSLETIEHYLDAGVSFVIIGTKAVQDPEFVEQACKQFAGHIIVGIDAKDGMVATDGWANVTDVKATDLAKRFADAG
VSSIVYTDIARDGMMQGVNVEQTVNLAQYSGLPVIASGGVTNLDDVRLLKGKPGILGAITGRAIYEGTLSLREAQLLLDQ
NTL
>Mature_243_residues
MLIIPAIDLKDGKCVRLKQGRMEDDTVFSDDPVATAQHWVNEGARRLHLVDLNGAFAGTPIHKPVVEAIAKAQPELPIQI
GGGIRSLETIEHYLDAGVSFVIIGTKAVQDPEFVEQACKQFAGHIIVGIDAKDGMVATDGWANVTDVKATDLAKRFADAG
VSSIVYTDIARDGMMQGVNVEQTVNLAQYSGLPVIASGGVTNLDDVRLLKGKPGILGAITGRAIYEGTLSLREAQLLLDQ
NTL

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=240, Percent_Identity=35, Blast_Score=138, Evalue=3e-34,
Organism=Escherichia coli, GI1788336, Length=247, Percent_Identity=25.5060728744939, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_ACIAD (Q6F7A5)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_047882.1
- ProteinModelPortal:   Q6F7A5
- SMR:   Q6F7A5
- STRING:   Q6F7A5
- GeneID:   2878923
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD3398
- NMPDR:   fig|62977.3.peg.3075
- eggNOG:   COG0106
- HOGENOM:   HBG541613
- OMA:   SIIYTDI
- PhylomeDB:   Q6F7A5
- ProtClustDB:   PRK00748
- BioCyc:   ASP62977:ACIAD3398-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00007

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 25926; Mature: 25926

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: ACT_SITE 8-8 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIIPAIDLKDGKCVRLKQGRMEDDTVFSDDPVATAQHWVNEGARRLHLVDLNGAFAGTP
CEEEEEEECCCCCEEEEECCCCCCCCEECCCCHHHHHHHHCCCCEEEEEEEECCCCCCCC
IHKPVVEAIAKAQPELPIQIGGGIRSLETIEHYLDAGVSFVIIGTKAVQDPEFVEQACKQ
CHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHH
FAGHIIVGIDAKDGMVATDGWANVTDVKATDLAKRFADAGVSSIVYTDIARDGMMQGVNV
HCCEEEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCH
EQTVNLAQYSGLPVIASGGVTNLDDVRLLKGKPGILGAITGRAIYEGTLSLREAQLLLDQ
HHHHHHHHHCCCCEEECCCCCCCCCEEEECCCCCEEEEECCCEEEHHHHHHHHHHHHHCC
NTL
CCC
>Mature Secondary Structure
MLIIPAIDLKDGKCVRLKQGRMEDDTVFSDDPVATAQHWVNEGARRLHLVDLNGAFAGTP
CEEEEEEECCCCCEEEEECCCCCCCCEECCCCHHHHHHHHCCCCEEEEEEEECCCCCCCC
IHKPVVEAIAKAQPELPIQIGGGIRSLETIEHYLDAGVSFVIIGTKAVQDPEFVEQACKQ
CHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHH
FAGHIIVGIDAKDGMVATDGWANVTDVKATDLAKRFADAGVSSIVYTDIARDGMMQGVNV
HCCEEEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCH
EQTVNLAQYSGLPVIASGGVTNLDDVRLLKGKPGILGAITGRAIYEGTLSLREAQLLLDQ
HHHHHHHHHCCCCEEECCCCCCCCCEEEECCCCCEEEEECCCEEEHHHHHHHHHHHHHCC
NTL
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA