Click here to switch to the map view.

The map label for this gene is murF [H]

Identifier: 50086342

GI number: 50086342

Start: 3272225

End: 3273631

Strand: Reverse

Name: murF [H]

Synonym: ACIAD3364

Alternate gene names: 50086342

Gene position: 3273631-3272225 (Counterclockwise)

Preceding gene: 50086343

Following gene: 50086341

Centisome position: 90.97

GC content: 46.06

Gene sequence:

>1407_bases
ATGCATACTTCTACAACCAGCACGGTTCCTTTAGCACCGTGGGATGCTTCTCAGTTACAACAGGCAACGCAAGGCTACTG
GCATAATAATCGCCAGCCTGAAGGCACGATCAAACGAATTTTGACAGATTCGCGTCATGCAGAGGCGGGAGATGCATTCT
TGGCGCTCAAAGGTGAACGTTTTGATGCGCATGATTTTGTCGCTCAGGTGGTTCAGCAAGGTTGTCAGGTTGCGATTGTG
GAACAACCATTAGATGTAGATATTTTTCAACTGATCGTATCAGATACACGTCTGGCACTTGGTGCTTTGGGCGCGTATCG
TCGTCAGCAATATCCAAAGCTTCAAGTAATTGCCTTAACTGGAAGTAGTGGTAAAACCACAACCAAAGAAATGCTGGGAA
GTATTCTGTCCCGTTTGGCTCCAACTTTGGTCACACGTGGAAATCTGAATAATGATCTGGGTGTTCCCATGATGCTACTT
GAGTTAAGGCCAGAACATCAGTATGCAGTTATGGAGCTGGGTGCCAGTCATCAGGGAGAAATTGACTATACCTCAAAACT
GGTTCAGCCTCATGTGGCGGGTATTTTAAATATCGGAACAGCACATTTGGGGGAGTTTGGAGGGCGTGATGGAATCTGTC
GGGCTAAATCCGAGATTTATCAACATATTGCAGTAGATGGAACCGCAATTTTACCTGCAGATGATGATTTTACCGACCAA
ATCAAACAGGCGATTCAGACTCAAAAAATGCTCAGTTTTGGTCAGGATGGTGAGATTTACGCGACTTCTGTCGTGCTGCA
TGCTCAATCGAGTGAATTTGACTTACATACACCTCAAGGTCAGGCACATCTCAGCTTGCCATTTGCAGGTGAGCATAATG
TACAAAATGCACTCGCGGCGACTGCTTTTGCGCTCGCGATTGGAATCGATTTGAATAATATCGTGCAAGGGCTTTCAGCG
GCACAAGGTGCAAAAGGGCGTTTGAACTTTATTCAGCACGGTCATCATCTATTTATTGATGATACCTATAATGCCAATCC
TGGCTCCATGCGTGCTGCTGCGGGGGTGCTTGCTCAGCAAACTGGAATTAAAGTGATGGTGACGGGGGACATTGGTGAGC
TCGGAGATGCCAGTATGCTGGAACACACCCAACTGGGGCATGATCTGGCAAATGAGCAGCTTGACTATATTGTTGCGGTA
GGAGAATTTGCTGGGGCGATGCAGCAGGGTGCTGCGAGCAGTGATCATCCAGAAAAATTGAAGGCATTTACCACTCAGGC
CCAAGCACTTCCTTTTTTAATTGAATTAATTCAACAACATCAACCCCAGTCCATGAGTTTCCTGTTTAAAGGTTCTCGTT
ATACCCATATGGAAACGTTGATGGCTGACTTGATGGAGAAACTTTAA

Upstream 100 bases:

>100_bases
GTGTTCGTCACTGGTTTGATGATGTAACAGAAGTCCAGTCCGCTATAAATACACAAACTCATTCAGTTCATTCTGCGTAT
CCTGCACATTAGGATTTTTT

Downstream 100 bases:

>100_bases
ATGCTGTTATGGTTGTTTGAACATCTATCGGGCTACAACAGCTCGTTCCAAGTTATTCGTTATTTAACATTACGTTCATT
GCTAAGTATTTTGACGTCAC

Product: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase (UDP-MurNAc-pentapeptide synthetase) (D-alanyl-D-alanine-adding enzyme)

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MHTSTTSTVPLAPWDASQLQQATQGYWHNNRQPEGTIKRILTDSRHAEAGDAFLALKGERFDAHDFVAQVVQQGCQVAIV
EQPLDVDIFQLIVSDTRLALGALGAYRRQQYPKLQVIALTGSSGKTTTKEMLGSILSRLAPTLVTRGNLNNDLGVPMMLL
ELRPEHQYAVMELGASHQGEIDYTSKLVQPHVAGILNIGTAHLGEFGGRDGICRAKSEIYQHIAVDGTAILPADDDFTDQ
IKQAIQTQKMLSFGQDGEIYATSVVLHAQSSEFDLHTPQGQAHLSLPFAGEHNVQNALAATAFALAIGIDLNNIVQGLSA
AQGAKGRLNFIQHGHHLFIDDTYNANPGSMRAAAGVLAQQTGIKVMVTGDIGELGDASMLEHTQLGHDLANEQLDYIVAV
GEFAGAMQQGAASSDHPEKLKAFTTQAQALPFLIELIQQHQPQSMSFLFKGSRYTHMETLMADLMEKL

Sequences:

>Translated_468_residues
MHTSTTSTVPLAPWDASQLQQATQGYWHNNRQPEGTIKRILTDSRHAEAGDAFLALKGERFDAHDFVAQVVQQGCQVAIV
EQPLDVDIFQLIVSDTRLALGALGAYRRQQYPKLQVIALTGSSGKTTTKEMLGSILSRLAPTLVTRGNLNNDLGVPMMLL
ELRPEHQYAVMELGASHQGEIDYTSKLVQPHVAGILNIGTAHLGEFGGRDGICRAKSEIYQHIAVDGTAILPADDDFTDQ
IKQAIQTQKMLSFGQDGEIYATSVVLHAQSSEFDLHTPQGQAHLSLPFAGEHNVQNALAATAFALAIGIDLNNIVQGLSA
AQGAKGRLNFIQHGHHLFIDDTYNANPGSMRAAAGVLAQQTGIKVMVTGDIGELGDASMLEHTQLGHDLANEQLDYIVAV
GEFAGAMQQGAASSDHPEKLKAFTTQAQALPFLIELIQQHQPQSMSFLFKGSRYTHMETLMADLMEKL
>Mature_468_residues
MHTSTTSTVPLAPWDASQLQQATQGYWHNNRQPEGTIKRILTDSRHAEAGDAFLALKGERFDAHDFVAQVVQQGCQVAIV
EQPLDVDIFQLIVSDTRLALGALGAYRRQQYPKLQVIALTGSSGKTTTKEMLGSILSRLAPTLVTRGNLNNDLGVPMMLL
ELRPEHQYAVMELGASHQGEIDYTSKLVQPHVAGILNIGTAHLGEFGGRDGICRAKSEIYQHIAVDGTAILPADDDFTDQ
IKQAIQTQKMLSFGQDGEIYATSVVLHAQSSEFDLHTPQGQAHLSLPFAGEHNVQNALAATAFALAIGIDLNNIVQGLSA
AQGAKGRLNFIQHGHHLFIDDTYNANPGSMRAAAGVLAQQTGIKVMVTGDIGELGDASMLEHTQLGHDLANEQLDYIVAV
GEFAGAMQQGAASSDHPEKLKAFTTQAQALPFLIELIQQHQPQSMSFLFKGSRYTHMETLMADLMEKL

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=436, Percent_Identity=42.6605504587156, Blast_Score=307, Evalue=8e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 50716; Mature: 50716

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTSTTSTVPLAPWDASQLQQATQGYWHNNRQPEGTIKRILTDSRHAEAGDAFLALKGER
CCCCCCCEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCC
FDAHDFVAQVVQQGCQVAIVEQPLDVDIFQLIVSDTRLALGALGAYRRQQYPKLQVIALT
CCHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
GSSGKTTTKEMLGSILSRLAPTLVTRGNLNNDLGVPMMLLELRPEHQYAVMELGASHQGE
CCCCCCHHHHHHHHHHHHHHHHHEECCCCCCCCCCCEEEEEECCCCCCHHHHHCCCCCCC
IDYTSKLVQPHVAGILNIGTAHLGEFGGRDGICRAKSEIYQHIAVDGTAILPADDDFTDQ
CHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHH
IKQAIQTQKMLSFGQDGEIYATSVVLHAQSSEFDLHTPQGQAHLSLPFAGEHNVQNALAA
HHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCCCCCCCEEEECCCCCCCCHHHHHHH
TAFALAIGIDLNNIVQGLSAAQGAKGRLNFIQHGHHLFIDDTYNANPGSMRAAAGVLAQQ
HHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHC
TGIKVMVTGDIGELGDASMLEHTQLGHDLANEQLDYIVAVGEFAGAMQQGAASSDHPEKL
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCHHEEEEHHHHHHHHHHCCCCCCCHHHH
KAFTTQAQALPFLIELIQQHQPQSMSFLFKGSRYTHMETLMADLMEKL
HHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHC
>Mature Secondary Structure
MHTSTTSTVPLAPWDASQLQQATQGYWHNNRQPEGTIKRILTDSRHAEAGDAFLALKGER
CCCCCCCEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCC
FDAHDFVAQVVQQGCQVAIVEQPLDVDIFQLIVSDTRLALGALGAYRRQQYPKLQVIALT
CCHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
GSSGKTTTKEMLGSILSRLAPTLVTRGNLNNDLGVPMMLLELRPEHQYAVMELGASHQGE
CCCCCCHHHHHHHHHHHHHHHHHEECCCCCCCCCCCEEEEEECCCCCCHHHHHCCCCCCC
IDYTSKLVQPHVAGILNIGTAHLGEFGGRDGICRAKSEIYQHIAVDGTAILPADDDFTDQ
CHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHH
IKQAIQTQKMLSFGQDGEIYATSVVLHAQSSEFDLHTPQGQAHLSLPFAGEHNVQNALAA
HHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCCCCCCCEEEECCCCCCCCHHHHHHH
TAFALAIGIDLNNIVQGLSAAQGAKGRLNFIQHGHHLFIDDTYNANPGSMRAAAGVLAQQ
HHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHC
TGIKVMVTGDIGELGDASMLEHTQLGHDLANEQLDYIVAVGEFAGAMQQGAASSDHPEKL
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCHHEEEEHHHHHHHHHHCCCCCCCHHHH
KAFTTQAQALPFLIELIQQHQPQSMSFLFKGSRYTHMETLMADLMEKL
HHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2668880; 8973200; 1630901; 9278503; 9166795; 2186811; 11090285 [H]