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The map label for this gene is spoT [H]

Identifier: 50086309

GI number: 50086309

Start: 3229846

End: 3231960

Strand: Direct

Name: spoT [H]

Synonym: ACIAD3326

Alternate gene names: 50086309

Gene position: 3229846-3231960 (Clockwise)

Preceding gene: 50086308

Following gene: 50086310

Centisome position: 89.75

GC content: 41.84

Gene sequence:

>2115_bases
ATGCCAGGTGAAGAGGTCAGCCAAGCTAAGCAACAGCTTAAATTAATTATTGATGCCTACTTAAGTGAAAGTGACGTTGA
TAATGTGCTTGCTGCCTGCGATTTTGCCGATCTCGCACACAGCGGTATAACACGTAAAAGCGGCGAACCATATATTCTAC
ATCCGATTGCAGTCAGTTGTATTCTGGCACACATGCGTCTGGATGCTGAAACCCTGATGGCGGCACTATTACATGATGTC
ATCGAAGATACCGAACACACCAAAGATGAAATTGGTGAGCGTTTTGGTGAAGTTGTGGCTGATCTGGTGGATGGGGTAAC
TAAACTCACTCATTCAAGCGATAAGCAATATAATAAGGCGGCTTCTTTTCGGAAAATTCTGCAAGCGACGCTGCAAGATC
CACGAGTCATTATTATCAAGCTCGCAGACCGCTATCATAACATGACTACACTGGATGCCCTGCGACCAGACAAACGTGCA
CGCATTGCGCAAGAAACGTTTGATATATTTGTGCCTATGGCCCGTATCGTGGGCATGAATGAAATGGGCGATAATCTTGA
ATTATTGTGTTATCAGAATCTTGATTTAGATATGTACAATAATGTGCAAGAAGCACTCCTTGAAACCAAACCAAGACGTT
GTGAATATCAGACCAAATGGGAACATCATCTAGGTGAATTACTTACCACTTATCACATCCATGGTCGCGTTAAAAAGAAA
AATAATAATATTGAGCTATTACGCCATTTTGTAAAAAATGAAATCGATCTTCAGGAACTTACCCATAGCCATGCTTTTGA
AATTATTTTAAATAGTATTTCAGATTGTGATCGTCTGGTGGATGCCTTAAAAGAAAATTTCCAGATTTTACATTTTGAAG
ATCATATTCGACGCCCATTACCTGGTGGCAATCAGTCGATCATGATGAAGTTAAAAGGTGAACAAACAACGTTGTCACTC
ACCATCCAGACTGAACTGATGCGTAAAGCTGCACGCTTTGGTGTGGTACTCGGTGAAAATGCACCGCAAGCTTGCCGCTC
CGCGATTCAGGCCTCAATGCAGAATCTCACCACTTTAATTGATGGTGAATGCGCTAAAACGACGTTCAATGATTTGCTGG
ACTATTTGCATCAAGAAAAAATCTGGGTATATACCCCGCATGGTCATTTGCACGAATTACCGCAAGGTGCGACCGTAATT
GATTTTGCCTATTCAGCCAGTTTATTTCTTGGCAACCATGCTGTAGGTGCCAAAATTGACGGTGAAATCCGACCGCTTTC
GACACCACTGGTCAGTGGTCAAGTGATTGATGTTATTACCGATGTGCTCGCAACTCCTAATCCTGACTGGCTCAGCTTCA
TCAATACACAAAAAGCACGACGCGCAATACAAAATATGCTTCGTGATCAGGATATTGAAGAACAGCTTCTGGTTGGACAG
CAAGCGTTAAATCGTGCACTCAAATTATTTAATCGTTCTATGCATGATTTATCCGAATCTGATTGGTTAAATGTCTTGCA
ATGGCGTCATGTCGAAAATAAAGAAGCGCTGTTTGAACAAATTGCGGTAGGCGATTTGCTGCCACAACTGGTTGCCAATC
ATTTGTATGCTCAAGATACCTCTGATAAAACAGGACATGCGCCTGATCGTCTGATTCAGGGAACAGAAGGGGTCGATGTC
AAATATGCACATTGTTGTAATCCAATTTTAGGTGATCCAATACAAGGGCATTTATCACGTCGTGGATTGATTGTACATCG
GGCTCGTTGCCATAACTTATTACATGAACAACACCTTCATCCTGAAAACATCATGCCACTGCAATGGACATCCGATGATT
TAGACGATGTCAGTTTTACTGCCTATCTCTGTATTGACATGCCACTTGATGGTGAACAGATCTCAGAATTAATTTATTTA
TGCCGTAAGTCACATACAGGCGTAGAAATGGTGCAACCACATGGGGATCATACCTATGTGAATATCGTGGTCAATAATCG
TAAAGTAGTGGCACAAATTATTCGTGATTTGCGTATGCTGTTTGGCTTTCCTCGTGTTTCCCGCTTGTTTACTCCTGCTG
CTGTGACGGAATCTGTAAAAATTAAAGTAAGTTAA

Upstream 100 bases:

>100_bases
AAAAATTATGCGATTTTTTTTGTGAACAAATTGACAAGTTTCGCAATATGCTTTTCATTAAGAGAGATAAATTTTATCTC
CCATATATAAAAGGTGTTTT

Downstream 100 bases:

>100_bases
TCAATTATATCGATGATACTTTTGTCATGTGGTTTCATCGATTATGATGTTTTTATATAGAGAAAAGGAGTAATCGATGT
CACGCCAAGTTATTCATACT

Product: bifunctional guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase/(p)ppGpp synthetase II

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 704; Mature: 703

Protein sequence:

>704_residues
MPGEEVSQAKQQLKLIIDAYLSESDVDNVLAACDFADLAHSGITRKSGEPYILHPIAVSCILAHMRLDAETLMAALLHDV
IEDTEHTKDEIGERFGEVVADLVDGVTKLTHSSDKQYNKAASFRKILQATLQDPRVIIIKLADRYHNMTTLDALRPDKRA
RIAQETFDIFVPMARIVGMNEMGDNLELLCYQNLDLDMYNNVQEALLETKPRRCEYQTKWEHHLGELLTTYHIHGRVKKK
NNNIELLRHFVKNEIDLQELTHSHAFEIILNSISDCDRLVDALKENFQILHFEDHIRRPLPGGNQSIMMKLKGEQTTLSL
TIQTELMRKAARFGVVLGENAPQACRSAIQASMQNLTTLIDGECAKTTFNDLLDYLHQEKIWVYTPHGHLHELPQGATVI
DFAYSASLFLGNHAVGAKIDGEIRPLSTPLVSGQVIDVITDVLATPNPDWLSFINTQKARRAIQNMLRDQDIEEQLLVGQ
QALNRALKLFNRSMHDLSESDWLNVLQWRHVENKEALFEQIAVGDLLPQLVANHLYAQDTSDKTGHAPDRLIQGTEGVDV
KYAHCCNPILGDPIQGHLSRRGLIVHRARCHNLLHEQHLHPENIMPLQWTSDDLDDVSFTAYLCIDMPLDGEQISELIYL
CRKSHTGVEMVQPHGDHTYVNIVVNNRKVVAQIIRDLRMLFGFPRVSRLFTPAAVTESVKIKVS

Sequences:

>Translated_704_residues
MPGEEVSQAKQQLKLIIDAYLSESDVDNVLAACDFADLAHSGITRKSGEPYILHPIAVSCILAHMRLDAETLMAALLHDV
IEDTEHTKDEIGERFGEVVADLVDGVTKLTHSSDKQYNKAASFRKILQATLQDPRVIIIKLADRYHNMTTLDALRPDKRA
RIAQETFDIFVPMARIVGMNEMGDNLELLCYQNLDLDMYNNVQEALLETKPRRCEYQTKWEHHLGELLTTYHIHGRVKKK
NNNIELLRHFVKNEIDLQELTHSHAFEIILNSISDCDRLVDALKENFQILHFEDHIRRPLPGGNQSIMMKLKGEQTTLSL
TIQTELMRKAARFGVVLGENAPQACRSAIQASMQNLTTLIDGECAKTTFNDLLDYLHQEKIWVYTPHGHLHELPQGATVI
DFAYSASLFLGNHAVGAKIDGEIRPLSTPLVSGQVIDVITDVLATPNPDWLSFINTQKARRAIQNMLRDQDIEEQLLVGQ
QALNRALKLFNRSMHDLSESDWLNVLQWRHVENKEALFEQIAVGDLLPQLVANHLYAQDTSDKTGHAPDRLIQGTEGVDV
KYAHCCNPILGDPIQGHLSRRGLIVHRARCHNLLHEQHLHPENIMPLQWTSDDLDDVSFTAYLCIDMPLDGEQISELIYL
CRKSHTGVEMVQPHGDHTYVNIVVNNRKVVAQIIRDLRMLFGFPRVSRLFTPAAVTESVKIKVS
>Mature_703_residues
PGEEVSQAKQQLKLIIDAYLSESDVDNVLAACDFADLAHSGITRKSGEPYILHPIAVSCILAHMRLDAETLMAALLHDVI
EDTEHTKDEIGERFGEVVADLVDGVTKLTHSSDKQYNKAASFRKILQATLQDPRVIIIKLADRYHNMTTLDALRPDKRAR
IAQETFDIFVPMARIVGMNEMGDNLELLCYQNLDLDMYNNVQEALLETKPRRCEYQTKWEHHLGELLTTYHIHGRVKKKN
NNIELLRHFVKNEIDLQELTHSHAFEIILNSISDCDRLVDALKENFQILHFEDHIRRPLPGGNQSIMMKLKGEQTTLSLT
IQTELMRKAARFGVVLGENAPQACRSAIQASMQNLTTLIDGECAKTTFNDLLDYLHQEKIWVYTPHGHLHELPQGATVID
FAYSASLFLGNHAVGAKIDGEIRPLSTPLVSGQVIDVITDVLATPNPDWLSFINTQKARRAIQNMLRDQDIEEQLLVGQQ
ALNRALKLFNRSMHDLSESDWLNVLQWRHVENKEALFEQIAVGDLLPQLVANHLYAQDTSDKTGHAPDRLIQGTEGVDVK
YAHCCNPILGDPIQGHLSRRGLIVHRARCHNLLHEQHLHPENIMPLQWTSDDLDDVSFTAYLCIDMPLDGEQISELIYLC
RKSHTGVEMVQPHGDHTYVNIVVNNRKVVAQIIRDLRMLFGFPRVSRLFTPAAVTESVKIKVS

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Homo sapiens, GI38348360, Length=139, Percent_Identity=39.568345323741, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1790082, Length=703, Percent_Identity=33.1436699857752, Blast_Score=351, Evalue=8e-98,
Organism=Escherichia coli, GI1789147, Length=600, Percent_Identity=26.6666666666667, Blast_Score=192, Evalue=6e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 79741; Mature: 79610

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGEEVSQAKQQLKLIIDAYLSESDVDNVLAACDFADLAHSGITRKSGEPYILHPIAVSC
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHH
ILAHMRLDAETLMAALLHDVIEDTEHTKDEIGERFGEVVADLVDGVTKLTHSSDKQYNKA
HHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ASFRKILQATLQDPRVIIIKLADRYHNMTTLDALRPDKRARIAQETFDIFVPMARIVGMN
HHHHHHHHHHHCCCCEEEEEEHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC
EMGDNLELLCYQNLDLDMYNNVQEALLETKPRRCEYQTKWEHHLGELLTTYHIHGRVKKK
CCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCEEEEC
NNNIELLRHFVKNEIDLQELTHSHAFEIILNSISDCDRLVDALKENFQILHFEDHIRRPL
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHCCCC
PGGNQSIMMKLKGEQTTLSLTIQTELMRKAARFGVVLGENAPQACRSAIQASMQNLTTLI
CCCCCEEEEEEECCCEEEEEEEHHHHHHHHHHHCEEECCCCCHHHHHHHHHHHHHHHHHH
DGECAKTTFNDLLDYLHQEKIWVYTPHGHLHELPQGATVIDFAYSASLFLGNHAVGAKID
CCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHCCCCCEEEEHHHHHHHEECCCCCCCEEC
GEIRPLSTPLVSGQVIDVITDVLATPNPDWLSFINTQKARRAIQNMLRDQDIEEQLLVGQ
CCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
QALNRALKLFNRSMHDLSESDWLNVLQWRHVENKEALFEQIAVGDLLPQLVANHLYAQDT
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
SDKTGHAPDRLIQGTEGVDVKYAHCCNPILGDPIQGHLSRRGLIVHRARCHNLLHEQHLH
CCCCCCCHHHHHCCCCCCCEEHHHHCCCCCCCCHHHHHCCCCCEEEHHHHHHHHHHHCCC
PENIMPLQWTSDDLDDVSFTAYLCIDMPLDGEQISELIYLCRKSHTGVEMVQPHGDHTYV
CCCCCCCEECCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEECCCCCCEEE
NIVVNNRKVVAQIIRDLRMLFGFPRVSRLFTPAAVTESVKIKVS
EEEECCHHHHHHHHHHHHHHHCCHHHHHHCCCHHHCCCEEEEEC
>Mature Secondary Structure 
PGEEVSQAKQQLKLIIDAYLSESDVDNVLAACDFADLAHSGITRKSGEPYILHPIAVSC
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHH
ILAHMRLDAETLMAALLHDVIEDTEHTKDEIGERFGEVVADLVDGVTKLTHSSDKQYNKA
HHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ASFRKILQATLQDPRVIIIKLADRYHNMTTLDALRPDKRARIAQETFDIFVPMARIVGMN
HHHHHHHHHHHCCCCEEEEEEHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC
EMGDNLELLCYQNLDLDMYNNVQEALLETKPRRCEYQTKWEHHLGELLTTYHIHGRVKKK
CCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCEEEEC
NNNIELLRHFVKNEIDLQELTHSHAFEIILNSISDCDRLVDALKENFQILHFEDHIRRPL
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHCCCC
PGGNQSIMMKLKGEQTTLSLTIQTELMRKAARFGVVLGENAPQACRSAIQASMQNLTTLI
CCCCCEEEEEEECCCEEEEEEEHHHHHHHHHHHCEEECCCCCHHHHHHHHHHHHHHHHHH
DGECAKTTFNDLLDYLHQEKIWVYTPHGHLHELPQGATVIDFAYSASLFLGNHAVGAKID
CCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHCCCCCEEEEHHHHHHHEECCCCCCCEEC
GEIRPLSTPLVSGQVIDVITDVLATPNPDWLSFINTQKARRAIQNMLRDQDIEEQLLVGQ
CCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
QALNRALKLFNRSMHDLSESDWLNVLQWRHVENKEALFEQIAVGDLLPQLVANHLYAQDT
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
SDKTGHAPDRLIQGTEGVDVKYAHCCNPILGDPIQGHLSRRGLIVHRARCHNLLHEQHLH
CCCCCCCHHHHHCCCCCCCEEHHHHCCCCCCCCHHHHHCCCCCEEEHHHHHHHHHHHCCC
PENIMPLQWTSDDLDDVSFTAYLCIDMPLDGEQISELIYLCRKSHTGVEMVQPHGDHTYV
CCCCCCCEECCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEECCCCCCEEE
NIVVNNRKVVAQIIRDLRMLFGFPRVSRLFTPAAVTESVKIKVS
EEEECCHHHHHHHHHHHHHHHCCHHHHHHCCCHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]