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The map label for this gene is ispH

Identifier: 50086306

GI number: 50086306

Start: 3227449

End: 3228399

Strand: Reverse

Name: ispH

Synonym: ACIAD3322

Alternate gene names: 50086306

Gene position: 3228399-3227449 (Counterclockwise)

Preceding gene: 50086314

Following gene: 50086305

Centisome position: 89.71

GC content: 44.58

Gene sequence:

>951_bases
ATGGAAATTGTGTTGGCAAATCCGCGTGGTTTTTGTGCAGGTGTTGACCGTGCCATAGCAATCGTAAATCGCGCGCTCGA
ATGCTTTAATCCACCCATCTATGTGCGTCATGAAGTGGTACATAATAAGTTCGTGGTCGATGATCTGCGTCAGCGTGGCG
CAATTTTTGTTGATGAACTGGATCAGGTGCCTGACGATTCGATTGTTATTTTTAGCGCACATGGTGTATCTAAAGCTGTT
CAGCAAGAAGCAGAGCACCGTGGTTTAAAAGTATTTGATGCCACTTGTCCGCTGGTCACCAAGGTCCATATTGAAGTGAC
CAAATATGCACGTGAAGGTACCGAAGCGATTCTCATTGGTCATGAAGGACATCCAGAAGTTGAAGGTACCATGGGGCAAT
ATGACAAAAGTAAAGGTGGTCATATTTATCTGGTAGAAGATGAGGCTGATGTCGAAGCACTTGCTGTAAATCACCCAGAG
AAATTGGCCTTTGTCACCCAAACCACTTTATCCATTGATGATACAGCAAAAGTTATTGATGCCTTGCGAACCAAGTTCCC
CCAAATTCAGGGACCACGTAAAGACGATATCTGCTATGCCACGCAAAACCGTCAGGATGCAGTGCGTGATCTTGCAAGTC
GTTGTGATGTTGTGCTTGTTGTGGGTTCACCAAATTCATCTAACTCCAATCGTTTACGCGAATTGGCGGAACGTATGGGC
AAAGCTGCATATTTGGTCGATAATGCAGATCAGCTTGAACAGCAATGGTTTGATGGTGTGGCTAAAATTGGTGTAACCGC
AGGTGCATCGGCACCAGAAATCTTGATTAAACAAGTGATTCAGCGTTTACAGGACTGGGGTGCAGAAGCGCCAAAAGAGC
TTGATGGTCGTGAAGAAAATATCACGTTTAGTTTGCCTAAAGAATTAAGAATTCAAGTTACGCAAGCGTAA

Upstream 100 bases:

>100_bases
ATTGTAGTGTACTACGGGACTAAACTGAATATAGTCCACTATAAAAGTTTGTATAGATGCCAAATTTGAACGAAATATCT
AGCGAATAATTGAGGATTCT

Downstream 100 bases:

>100_bases
TGTGATTTTAATAATATTCTGAAGCGGATGAGTGTCACTCATCCGCTTTTTTATGCACTTAATCTTGATTGTATTGGTTA
TGTAAAAAAATAAGTTAAGC

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MEIVLANPRGFCAGVDRAIAIVNRALECFNPPIYVRHEVVHNKFVVDDLRQRGAIFVDELDQVPDDSIVIFSAHGVSKAV
QQEAEHRGLKVFDATCPLVTKVHIEVTKYAREGTEAILIGHEGHPEVEGTMGQYDKSKGGHIYLVEDEADVEALAVNHPE
KLAFVTQTTLSIDDTAKVIDALRTKFPQIQGPRKDDICYATQNRQDAVRDLASRCDVVLVVGSPNSSNSNRLRELAERMG
KAAYLVDNADQLEQQWFDGVAKIGVTAGASAPEILIKQVIQRLQDWGAEAPKELDGREENITFSLPKELRIQVTQA

Sequences:

>Translated_316_residues
MEIVLANPRGFCAGVDRAIAIVNRALECFNPPIYVRHEVVHNKFVVDDLRQRGAIFVDELDQVPDDSIVIFSAHGVSKAV
QQEAEHRGLKVFDATCPLVTKVHIEVTKYAREGTEAILIGHEGHPEVEGTMGQYDKSKGGHIYLVEDEADVEALAVNHPE
KLAFVTQTTLSIDDTAKVIDALRTKFPQIQGPRKDDICYATQNRQDAVRDLASRCDVVLVVGSPNSSNSNRLRELAERMG
KAAYLVDNADQLEQQWFDGVAKIGVTAGASAPEILIKQVIQRLQDWGAEAPKELDGREENITFSLPKELRIQVTQA
>Mature_316_residues
MEIVLANPRGFCAGVDRAIAIVNRALECFNPPIYVRHEVVHNKFVVDDLRQRGAIFVDELDQVPDDSIVIFSAHGVSKAV
QQEAEHRGLKVFDATCPLVTKVHIEVTKYAREGTEAILIGHEGHPEVEGTMGQYDKSKGGHIYLVEDEADVEALAVNHPE
KLAFVTQTTLSIDDTAKVIDALRTKFPQIQGPRKDDICYATQNRQDAVRDLASRCDVVLVVGSPNSSNSNRLRELAERMG
KAAYLVDNADQLEQQWFDGVAKIGVTAGASAPEILIKQVIQRLQDWGAEAPKELDGREENITFSLPKELRIQVTQA

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=315, Percent_Identity=64.4444444444444, Blast_Score=426, Evalue=1e-121,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_ACIAD (Q9RBJ0)

Other databases:

- EMBL:   AF027189
- EMBL:   CR543861
- RefSeq:   YP_047816.1
- STRING:   Q9RBJ0
- GeneID:   2879847
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD3322
- NMPDR:   fig|62977.3.peg.3009
- eggNOG:   COG0761
- HOGENOM:   HBG335228
- OMA:   HMQVARA
- PhylomeDB:   Q9RBJ0
- ProtClustDB:   PRK01045
- BioCyc:   ASP62977:ACIAD3322-MONOMER
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 34944; Mature: 34944

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIVLANPRGFCAGVDRAIAIVNRALECFNPPIYVRHEVVHNKFVVDDLRQRGAIFVDEL
CEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCEEEHHH
DQVPDDSIVIFSAHGVSKAVQQEAEHRGLKVFDATCPLVTKVHIEVTKYAREGTEAILIG
HCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEECCCCEEEEEEEEEEHHHCCCCCEEEEE
HEGHPEVEGTMGQYDKSKGGHIYLVEDEADVEALAVNHPEKLAFVTQTTLSIDDTAKVID
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEECCCCHHEEEEEEEEECCCHHHHHHH
ALRTKFPQIQGPRKDDICYATQNRQDAVRDLASRCDVVLVVGSPNSSNSNRLRELAERMG
HHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHC
KAAYLVDNADQLEQQWFDGVAKIGVTAGASAPEILIKQVIQRLQDWGAEAPKELDGREEN
CEEEEECCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHCCCCCCC
ITFSLPKELRIQVTQA
EEEECCCCEEEEEECC
>Mature Secondary Structure
MEIVLANPRGFCAGVDRAIAIVNRALECFNPPIYVRHEVVHNKFVVDDLRQRGAIFVDEL
CEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCEEEHHH
DQVPDDSIVIFSAHGVSKAVQQEAEHRGLKVFDATCPLVTKVHIEVTKYAREGTEAILIG
HCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEECCCCEEEEEEEEEEHHHCCCCCEEEEE
HEGHPEVEGTMGQYDKSKGGHIYLVEDEADVEALAVNHPEKLAFVTQTTLSIDDTAKVID
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEECCCCHHEEEEEEEEECCCHHHHHHH
ALRTKFPQIQGPRKDDICYATQNRQDAVRDLASRCDVVLVVGSPNSSNSNRLRELAERMG
HHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHC
KAAYLVDNADQLEQQWFDGVAKIGVTAGASAPEILIKQVIQRLQDWGAEAPKELDGREEN
CEEEEECCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHCCCCCCC
ITFSLPKELRIQVTQA
EEEECCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10763755