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The map label for this gene is gltT [H]

Identifier: 50086225

GI number: 50086225

Start: 3143711

End: 3144952

Strand: Reverse

Name: gltT [H]

Synonym: ACIAD3230

Alternate gene names: 50086225

Gene position: 3144952-3143711 (Counterclockwise)

Preceding gene: 50086226

Following gene: 50086217

Centisome position: 87.39

GC content: 42.59

Gene sequence:

>1242_bases
ATGAGCTTAAATACGCAAATTTTAATTGCGGCAATTTTGGGCATTGGATTCGGTTTTATCTTGAATCTTTATCCTCAGAC
CGCATTTTTTAGTACCAGTCTATATACGCTTGGAATCATCAGTAGCATTTTTATTGGCTTGCTGAAAATGCTATTGGTGC
CATTGATCTTTACCTCAATCGTGGTTGGAGTATCCAATTTACAGGCAAGTGGTCAGTTTGGCCGAGTGTGGAAGATCACC
GCACTTTGCTGTTTTGTCACCACAACGTTTGCATTGATTTTAGGTATCGGCTGTGCACATCTGTTTGGTGTGGGTAAAGG
CATGGATATTGCTATTTTCAGTGAAGCCATGAGTCAACATCAAGCTCCTGATACCTTATCACCGTCTGCATTTTTTACCA
ATTTTATTCAAAATACGCTCATTAACCCATTTAAAGCATTTAGTGAAGGGAATGTGCTGGCGGTTGTGGTCTTTGCTTTA
TTGATAGGTGCAGCACTGATTAAAGGCGGCGAACGTTTTCAGTCGGTACGCATACTGAGTTTACAACTGTTTGACATTAT
GATGATGTTGGTCGGTTGGGTTATGAAATTGGCGCCACTGGGTATTTTGGCTTTACTGGCCAAATTGATTGCAACAGAAG
ATTTAAAAATTTTAGGTAGCTTGGCAGAATTTGCTTTGGTGGTGACAGGCACGACGATTTTTCATGGTGCAGTGGTCTTG
CCACTGTTATTGTGGATCTTTGGCAAAATGAATCCAATCACCTTTTTCAAAGGCACGCGACTGGCTCTGATTACTGCATT
TGCAACCAGTTCGAGCTCGGCTACCATGCCCTTAAGTATGAAATGTGCGCAGGAAAATCTAAAGGTTCGCCCACAAACGG
CTGGTTTCGTCATTCCTTTAGGAACGCAGTTAAATATGGATGGCACGGCACTTTATGAAGCTGCTGCTGCTTTGTTTATT
GCCAATCTGGTGGGGCTGGATCTTAGCCTAACCCAACAACTGATTGTATGTTTAACTGCGATGATTGCCTCACTGGGTGC
ACCAGGTATTCCAAGTGCAGGTATGGTGACCATGATTATGGTATTGCAGTCGGTTGGTTTACCCGCAGAAGCGATTGCGA
TTTTACTCCCCATAGATCGAATTTTAGATACCGTACGTACGGTGGTGAATGTGCAGGGGGATATGATGATTAGTGTCGTT
GTTGACCGAAATATCAAAGTAATAGAAAGTTCAACTGAGTAA

Upstream 100 bases:

>100_bases
TGAAATTGAAACACAGTAATTTCAGCTTATAAATAAAACTCGATTCTTGATCTGGGTCATGTCATATTGCACTTAATCAA
ATAAAAGAATCGACTTAAAT

Downstream 100 bases:

>100_bases
TTTTTTTATCATTTTTTCTAAAAAAATGACTCAAAAAAGCAGGAGACTAATCCTGCTTTTTATTGGATTTGAGTATACAA
CCGCTTTATGCTGCAACTTT

Product: proton/sodium-glutamate symport protein

Products: L-aspartate [Cytoplasm]; Proton [Cytoplasm]; L-glutamate [Cytoplasm] [C]

Alternate protein names: Glutamate-aspartate carrier protein [H]

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MSLNTQILIAAILGIGFGFILNLYPQTAFFSTSLYTLGIISSIFIGLLKMLLVPLIFTSIVVGVSNLQASGQFGRVWKIT
ALCCFVTTTFALILGIGCAHLFGVGKGMDIAIFSEAMSQHQAPDTLSPSAFFTNFIQNTLINPFKAFSEGNVLAVVVFAL
LIGAALIKGGERFQSVRILSLQLFDIMMMLVGWVMKLAPLGILALLAKLIATEDLKILGSLAEFALVVTGTTIFHGAVVL
PLLLWIFGKMNPITFFKGTRLALITAFATSSSSATMPLSMKCAQENLKVRPQTAGFVIPLGTQLNMDGTALYEAAAALFI
ANLVGLDLSLTQQLIVCLTAMIASLGAPGIPSAGMVTMIMVLQSVGLPAEAIAILLPIDRILDTVRTVVNVQGDMMISVV
VDRNIKVIESSTE

Sequences:

>Translated_413_residues
MSLNTQILIAAILGIGFGFILNLYPQTAFFSTSLYTLGIISSIFIGLLKMLLVPLIFTSIVVGVSNLQASGQFGRVWKIT
ALCCFVTTTFALILGIGCAHLFGVGKGMDIAIFSEAMSQHQAPDTLSPSAFFTNFIQNTLINPFKAFSEGNVLAVVVFAL
LIGAALIKGGERFQSVRILSLQLFDIMMMLVGWVMKLAPLGILALLAKLIATEDLKILGSLAEFALVVTGTTIFHGAVVL
PLLLWIFGKMNPITFFKGTRLALITAFATSSSSATMPLSMKCAQENLKVRPQTAGFVIPLGTQLNMDGTALYEAAAALFI
ANLVGLDLSLTQQLIVCLTAMIASLGAPGIPSAGMVTMIMVLQSVGLPAEAIAILLPIDRILDTVRTVVNVQGDMMISVV
VDRNIKVIESSTE
>Mature_412_residues
SLNTQILIAAILGIGFGFILNLYPQTAFFSTSLYTLGIISSIFIGLLKMLLVPLIFTSIVVGVSNLQASGQFGRVWKITA
LCCFVTTTFALILGIGCAHLFGVGKGMDIAIFSEAMSQHQAPDTLSPSAFFTNFIQNTLINPFKAFSEGNVLAVVVFALL
IGAALIKGGERFQSVRILSLQLFDIMMMLVGWVMKLAPLGILALLAKLIATEDLKILGSLAEFALVVTGTTIFHGAVVLP
LLLWIFGKMNPITFFKGTRLALITAFATSSSSATMPLSMKCAQENLKVRPQTAGFVIPLGTQLNMDGTALYEAAAALFIA
NLVGLDLSLTQQLIVCLTAMIASLGAPGIPSAGMVTMIMVLQSVGLPAEAIAILLPIDRILDTVRTVVNVQGDMMISVVV
DRNIKVIESSTE

Specific function: This carrier protein is part of the Na(+)-dependent, binding-protein-independent glutamate-aspartate transport system [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI66773030, Length=281, Percent_Identity=43.4163701067616, Blast_Score=200, Evalue=2e-51,
Organism=Homo sapiens, GI4827012, Length=469, Percent_Identity=33.2622601279318, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI169790839, Length=447, Percent_Identity=32.8859060402685, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI40254478, Length=408, Percent_Identity=35.2941176470588, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI21314632, Length=403, Percent_Identity=31.2655086848635, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI5032093, Length=391, Percent_Identity=32.9923273657289, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI223468564, Length=266, Percent_Identity=38.7218045112782, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI223468566, Length=266, Percent_Identity=38.7218045112782, Blast_Score=176, Evalue=4e-44,
Organism=Homo sapiens, GI194239697, Length=252, Percent_Identity=41.2698412698413, Blast_Score=175, Evalue=7e-44,
Organism=Homo sapiens, GI262359914, Length=376, Percent_Identity=30.5851063829787, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI301601644, Length=144, Percent_Identity=37.5, Blast_Score=109, Evalue=5e-24,
Organism=Escherichia coli, GI1790514, Length=416, Percent_Identity=31.9711538461538, Blast_Score=174, Evalue=6e-45,
Organism=Escherichia coli, GI1789947, Length=416, Percent_Identity=28.125, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1788024, Length=403, Percent_Identity=28.7841191066998, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1789473, Length=370, Percent_Identity=25.1351351351351, Blast_Score=90, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71996953, Length=443, Percent_Identity=37.020316027088, Blast_Score=236, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI17537407, Length=417, Percent_Identity=35.0119904076739, Blast_Score=197, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI71983106, Length=435, Percent_Identity=33.1034482758621, Blast_Score=191, Evalue=7e-49,
Organism=Caenorhabditis elegans, GI71983099, Length=431, Percent_Identity=32.4825986078886, Blast_Score=191, Evalue=8e-49,
Organism=Caenorhabditis elegans, GI193206505, Length=420, Percent_Identity=35.9523809523809, Blast_Score=188, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI193206654, Length=444, Percent_Identity=28.8288288288288, Blast_Score=172, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17541374, Length=395, Percent_Identity=31.8987341772152, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24583025, Length=394, Percent_Identity=34.7715736040609, Blast_Score=186, Evalue=3e-47,
Organism=Drosophila melanogaster, GI17137668, Length=394, Percent_Identity=34.7715736040609, Blast_Score=186, Evalue=3e-47,
Organism=Drosophila melanogaster, GI24583023, Length=394, Percent_Identity=34.7715736040609, Blast_Score=186, Evalue=3e-47,
Organism=Drosophila melanogaster, GI281360481, Length=454, Percent_Identity=27.7533039647577, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI281360483, Length=454, Percent_Identity=27.7533039647577, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI17137666, Length=454, Percent_Identity=27.7533039647577, Blast_Score=118, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 43943; Mature: 43812

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLNTQILIAAILGIGFGFILNLYPQTAFFSTSLYTLGIISSIFIGLLKMLLVPLIFTSI
CCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVGVSNLQASGQFGRVWKITALCCFVTTTFALILGIGCAHLFGVGKGMDIAIFSEAMSQH
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHC
QAPDTLSPSAFFTNFIQNTLINPFKAFSEGNVLAVVVFALLIGAALIKGGERFQSVRILS
CCCCCCCHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
LQLFDIMMMLVGWVMKLAPLGILALLAKLIATEDLKILGSLAEFALVVTGTTIFHGAVVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
PLLLWIFGKMNPITFFKGTRLALITAFATSSSSATMPLSMKCAQENLKVRPQTAGFVIPL
HHHHHHHCCCCCEEEECCCCEEEEEEEECCCCCCCCCEEHHHHHCCCCCCCCCCCEEEEC
GTQLNMDGTALYEAAAALFIANLVGLDLSLTQQLIVCLTAMIASLGAPGIPSAGMVTMIM
CCEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
VLQSVGLPAEAIAILLPIDRILDTVRTVVNVQGDMMISVVVDRNIKVIESSTE
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCC
>Mature Secondary Structure 
SLNTQILIAAILGIGFGFILNLYPQTAFFSTSLYTLGIISSIFIGLLKMLLVPLIFTSI
CCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVGVSNLQASGQFGRVWKITALCCFVTTTFALILGIGCAHLFGVGKGMDIAIFSEAMSQH
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHC
QAPDTLSPSAFFTNFIQNTLINPFKAFSEGNVLAVVVFALLIGAALIKGGERFQSVRILS
CCCCCCCHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
LQLFDIMMMLVGWVMKLAPLGILALLAKLIATEDLKILGSLAEFALVVTGTTIFHGAVVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
PLLLWIFGKMNPITFFKGTRLALITAFATSSSSATMPLSMKCAQENLKVRPQTAGFVIPL
HHHHHHHCCCCCEEEECCCCEEEEEEEECCCCCCCCCEEHHHHHCCCCCCCCCCCEEEEC
GTQLNMDGTALYEAAAALFIANLVGLDLSLTQQLIVCLTAMIASLGAPGIPSAGMVTMIM
CCEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
VLQSVGLPAEAIAILLPIDRILDTVRTVVNVQGDMMISVVVDRNIKVIESSTE
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-aspartate [Periplasm]; Proton [Periplasm]; L-glutamate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + L-aspartate [Periplasm] = Proton [Cytoplasm] + L-aspartate [Cytoplasm] Proton [Periplasm] + L-glutamate [Periplasm] = Proton [Cytoplasm] + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]