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The map label for this gene is hmp [H]

Identifier: 50086221

GI number: 50086221

Start: 3140306

End: 3141070

Strand: Direct

Name: hmp [H]

Synonym: ACIAD3226

Alternate gene names: 50086221

Gene position: 3140306-3141070 (Clockwise)

Preceding gene: 50086220

Following gene: 50086222

Centisome position: 87.26

GC content: 40.13

Gene sequence:

>765_bases
ATGACACCACAACAAATTGAACTCGTAAAATCTACCGTACCTGTACTACGCGAACATGGCGTAACACTCACCAGCTATTT
TTATAATCGCATGCTCAACAATCACCCTGAATTAAAAAATGTGTTTAATTTAGATCATCAAAGCACAGGGCGACAACCTC
GTGCGCTGGCCGCCGCAGTACTGGCTTATGCTGAACATATCGAACAACCTGAAAAATTAGCCAAAGCAGTTGAACGGATT
ACCACCAAGCATGTCAGCCTAAATATCCAGCCAGAACAATATGCAATTGTGGGTGAAAATCTTCTACATTCGATTAGTGA
AGTATTGAACGTACCGTTCGAATCAGAATTGATTGAAGCGTGGAAACAGGCTTATTTACAACTGGCAGATATTTTAATTG
AGGTAGAAAAAGAGAAATATCATCAACTTGCCAAACAGGATGGCGGCTGGACTGGCTGGCGTAGCTTCAAGATCATTGCA
ATTCAAGAACAATATGAAGGCAAATGTTTTAAGATTTCAGCAACAGACCAACAGGCTATACTCGCTGCAACGTTAGGAGA
ATATATCTCTGTGAAAGTGCATGTGCCAGAAAAAGAACTCGATCAGCCTCAACAATTTAACCTGAATACTACGCAAGCTG
ATGATCATTATGAGTTTGTGGTTAAACCAGAACCTGCACATAGTGACTATGATGTCGCAAATATTTTACTAAATCATTAT
AGTCTAGGTGATGAGGTGATGGTTTCTGCACCCATTAGAAACTAA

Upstream 100 bases:

>100_bases
GATTCAAAAGCTTAAATCAAAGTATTAAAGATGCATTGCAAATACATCTTATAAAATGTATTTTGAATATATCTTATAAA
GCCAGACAAAAGGTCTCGCC

Downstream 100 bases:

>100_bases
TTTCAGTTTTTATGATCTGATCATCATCAAAATATGGCTTGGAAGTTTCTTTAAAGATACTTTCAAGCTAAGATTATTTT
TTGAATCATGATCTTTGCTC

Product: flavohemoprotein

Products: NA

Alternate protein names: Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MTPQQIELVKSTVPVLREHGVTLTSYFYNRMLNNHPELKNVFNLDHQSTGRQPRALAAAVLAYAEHIEQPEKLAKAVERI
TTKHVSLNIQPEQYAIVGENLLHSISEVLNVPFESELIEAWKQAYLQLADILIEVEKEKYHQLAKQDGGWTGWRSFKIIA
IQEQYEGKCFKISATDQQAILAATLGEYISVKVHVPEKELDQPQQFNLNTTQADDHYEFVVKPEPAHSDYDVANILLNHY
SLGDEVMVSAPIRN

Sequences:

>Translated_254_residues
MTPQQIELVKSTVPVLREHGVTLTSYFYNRMLNNHPELKNVFNLDHQSTGRQPRALAAAVLAYAEHIEQPEKLAKAVERI
TTKHVSLNIQPEQYAIVGENLLHSISEVLNVPFESELIEAWKQAYLQLADILIEVEKEKYHQLAKQDGGWTGWRSFKIIA
IQEQYEGKCFKISATDQQAILAATLGEYISVKVHVPEKELDQPQQFNLNTTQADDHYEFVVKPEPAHSDYDVANILLNHY
SLGDEVMVSAPIRN
>Mature_253_residues
TPQQIELVKSTVPVLREHGVTLTSYFYNRMLNNHPELKNVFNLDHQSTGRQPRALAAAVLAYAEHIEQPEKLAKAVERIT
TKHVSLNIQPEQYAIVGENLLHSISEVLNVPFESELIEAWKQAYLQLADILIEVEKEKYHQLAKQDGGWTGWRSFKIIAI
QEQYEGKCFKISATDQQAILAATLGEYISVKVHVPEKELDQPQQFNLNTTQADDHYEFVVKPEPAHSDYDVANILLNHYS
LGDEVMVSAPIRN

Specific function: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a centra

COG id: COG1017

COG function: function code C; Hemoglobin-like flavoprotein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788903, Length=252, Percent_Identity=38.0952380952381, Blast_Score=158, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6321673, Length=256, Percent_Identity=37.109375, Blast_Score=135, Evalue=5e-33,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017927
- InterPro:   IPR001709
- InterPro:   IPR012292
- InterPro:   IPR009050
- InterPro:   IPR000971
- InterPro:   IPR001834
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00042 Globin; PF00175 NAD_binding_1 [H]

EC number: =1.14.12.17 [H]

Molecular weight: Translated: 28914; Mature: 28783

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00133 CARBOXYPEPT_ZN_2 ; PS01033 GLOBIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPQQIELVKSTVPVLREHGVTLTSYFYNRMLNNHPELKNVFNLDHQSTGRQPRALAAAV
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCHHHHHHHH
LAYAEHIEQPEKLAKAVERITTKHVSLNIQPEQYAIVGENLLHSISEVLNVPFESELIEA
HHHHHHCCCHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHH
WKQAYLQLADILIEVEKEKYHQLAKQDGGWTGWRSFKIIAIQEQYEGKCFKISATDQQAI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCEEEEEECCCCHHH
LAATLGEYISVKVHVPEKELDQPQQFNLNTTQADDHYEFVVKPEPAHSDYDVANILLNHY
HHHHHHHHEEEEEECCHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHH
SLGDEVMVSAPIRN
CCCCCEEEECCCCC
>Mature Secondary Structure 
TPQQIELVKSTVPVLREHGVTLTSYFYNRMLNNHPELKNVFNLDHQSTGRQPRALAAAV
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCHHHHHHHH
LAYAEHIEQPEKLAKAVERITTKHVSLNIQPEQYAIVGENLLHSISEVLNVPFESELIEA
HHHHHHCCCHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHH
WKQAYLQLADILIEVEKEKYHQLAKQDGGWTGWRSFKIIAIQEQYEGKCFKISATDQQAI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCEEEEEECCCCHHH
LAATLGEYISVKVHVPEKELDQPQQFNLNTTQADDHYEFVVKPEPAHSDYDVANILLNHY
HHHHHHHHEEEEEECCHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHH
SLGDEVMVSAPIRN
CCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10484179; 11058132 [H]