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The map label for this gene is ynfL [H]

Identifier: 50086220

GI number: 50086220

Start: 3139321

End: 3140220

Strand: Direct

Name: ynfL [H]

Synonym: ACIAD3225

Alternate gene names: 50086220

Gene position: 3139321-3140220 (Clockwise)

Preceding gene: 50086219

Following gene: 50086221

Centisome position: 87.24

GC content: 37.0

Gene sequence:

>900_bases
ATGTCAACTTTAAAGCAATTCCGCTATTTCGTTACTATTGTAGAAACTGGAAGTTTCATTGCTGCTTCTGAACAATTATT
TATTGCTCAATCCGCTTTGAGTAGACAGATGATGTTGCTTGAAGATGAGCTTGGCTTTCTAATTTTTGATCGAAGTGAGA
AAAAAATAAAGTTGACCACTGCGGGGGATCAATTTTATCGAGATATCAAGAAACAACTTTCAGCGCTTCAAAATACAATT
CTCTCCGCTCAGAATCTTGCTAAGGGTAAAGGACGAATCTTTAGAATTGCGCATTCGAGTAGCGTAATACTCAACCAAGA
TAAACTGAAGCTATTTAAGCAGTTATGTATTGAATACCAGATCGAGATTGAAATTGAGACACTCGCTTCAGAATTACAGG
TTGAAGCTATTGAAAATGGTAGCTTGGATATCGGATTTATCCGTCCACCTGTTTTAGCGTCTCTCGAACATCTAAATCAT
CAAACTGTTTACAGCAGCAAGCTTTGTGTTGCGGTATGCATGGAACAACCAGCATTAAATCAACAAAAAGTGAAACTGTC
TGACTTAAAAGATATTCCTTTTGTTTCAATGCCACATAGTGACCGTGGAGGTTTGAGTTATTTAGCTGCCAATTTATGCT
TAAGTCATGGTTTTTCACCGCAACCTGCATTGATTCGTTCCAGAAAAATTACTCAACTCCAGTTAGTTTCACAAAACCTT
GGCATATGCATTGTGCCTCAAGATTTTGAAAGTGTACTTCCTAATAACGTGCGTTTAATTGAACTCGATCATGAATCACG
TTTTTCTCCTGTAGTTTTGCTATGGAAAAAAGAAACCGACTCACTACTGCAATGCTGTGTCGAAGAGATATTTAAATCTT
TCTATGATTCAAAAGCTTAA

Upstream 100 bases:

>100_bases
CGCATGCGATTGTCCTAAAGCTTTTCTTAGGAGCTAGCATATAAAAACCTTTTGATCTCATCTAATATAGAAAAATCCCC
CATCTATCTCATTTTTAGAT

Downstream 100 bases:

>100_bases
ATCAAAGTATTAAAGATGCATTGCAAATACATCTTATAAAATGTATTTTGAATATATCTTATAAAGCCAGACAAAAGGTC
TCGCCATGACACCACAACAA

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MSTLKQFRYFVTIVETGSFIAASEQLFIAQSALSRQMMLLEDELGFLIFDRSEKKIKLTTAGDQFYRDIKKQLSALQNTI
LSAQNLAKGKGRIFRIAHSSSVILNQDKLKLFKQLCIEYQIEIEIETLASELQVEAIENGSLDIGFIRPPVLASLEHLNH
QTVYSSKLCVAVCMEQPALNQQKVKLSDLKDIPFVSMPHSDRGGLSYLAANLCLSHGFSPQPALIRSRKITQLQLVSQNL
GICIVPQDFESVLPNNVRLIELDHESRFSPVVLLWKKETDSLLQCCVEEIFKSFYDSKA

Sequences:

>Translated_299_residues
MSTLKQFRYFVTIVETGSFIAASEQLFIAQSALSRQMMLLEDELGFLIFDRSEKKIKLTTAGDQFYRDIKKQLSALQNTI
LSAQNLAKGKGRIFRIAHSSSVILNQDKLKLFKQLCIEYQIEIEIETLASELQVEAIENGSLDIGFIRPPVLASLEHLNH
QTVYSSKLCVAVCMEQPALNQQKVKLSDLKDIPFVSMPHSDRGGLSYLAANLCLSHGFSPQPALIRSRKITQLQLVSQNL
GICIVPQDFESVLPNNVRLIELDHESRFSPVVLLWKKETDSLLQCCVEEIFKSFYDSKA
>Mature_298_residues
STLKQFRYFVTIVETGSFIAASEQLFIAQSALSRQMMLLEDELGFLIFDRSEKKIKLTTAGDQFYRDIKKQLSALQNTIL
SAQNLAKGKGRIFRIAHSSSVILNQDKLKLFKQLCIEYQIEIEIETLASELQVEAIENGSLDIGFIRPPVLASLEHLNHQ
TVYSSKLCVAVCMEQPALNQQKVKLSDLKDIPFVSMPHSDRGGLSYLAANLCLSHGFSPQPALIRSRKITQLQLVSQNLG
ICIVPQDFESVLPNNVRLIELDHESRFSPVVLLWKKETDSLLQCCVEEIFKSFYDSKA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=284, Percent_Identity=28.5211267605634, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1788887, Length=244, Percent_Identity=25, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI157672245, Length=191, Percent_Identity=27.7486910994764, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786401, Length=107, Percent_Identity=31.7757009345794, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI2367136, Length=257, Percent_Identity=24.5136186770428, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33758; Mature: 33626

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLKQFRYFVTIVETGSFIAASEQLFIAQSALSRQMMLLEDELGFLIFDRSEKKIKLTT
CCHHHHHHEEEEEEECCCEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEE
AGDQFYRDIKKQLSALQNTILSAQNLAKGKGRIFRIAHSSSVILNQDKLKLFKQLCIEYQ
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEECHHHHHHHHHHHHEEE
IEIEIETLASELQVEAIENGSLDIGFIRPPVLASLEHLNHQTVYSSKLCVAVCMEQPALN
EEEEHHHHHHHHEEEEECCCCEEEEEECCHHHHHHHHCCCHHHHHCCHHHEEECCCCCCC
QQKVKLSDLKDIPFVSMPHSDRGGLSYLAANLCLSHGFSPQPALIRSRKITQLQLVSQNL
CCCEEHHCCCCCCEEECCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCHHEEEHHCCC
GICIVPQDFESVLPNNVRLIELDHESRFSPVVLLWKKETDSLLQCCVEEIFKSFYDSKA
CEEEECCCHHHHCCCCEEEEEECCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
STLKQFRYFVTIVETGSFIAASEQLFIAQSALSRQMMLLEDELGFLIFDRSEKKIKLTT
CHHHHHHEEEEEEECCCEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEE
AGDQFYRDIKKQLSALQNTILSAQNLAKGKGRIFRIAHSSSVILNQDKLKLFKQLCIEYQ
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEECHHHHHHHHHHHHEEE
IEIEIETLASELQVEAIENGSLDIGFIRPPVLASLEHLNHQTVYSSKLCVAVCMEQPALN
EEEEHHHHHHHHEEEEECCCCEEEEEECCHHHHHHHHCCCHHHHHCCHHHEEECCCCCCC
QQKVKLSDLKDIPFVSMPHSDRGGLSYLAANLCLSHGFSPQPALIRSRKITQLQLVSQNL
CCCEEHHCCCCCCEEECCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCHHEEEHHCCC
GICIVPQDFESVLPNNVRLIELDHESRFSPVVLLWKKETDSLLQCCVEEIFKSFYDSKA
CEEEECCCHHHHCCCCEEEEEECCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]