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The map label for this gene is dmlR [H]

Identifier: 50086132

GI number: 50086132

Start: 3055982

End: 3056911

Strand: Direct

Name: dmlR [H]

Synonym: ACIAD3129

Alternate gene names: 50086132

Gene position: 3055982-3056911 (Clockwise)

Preceding gene: 50086130

Following gene: 50086133

Centisome position: 84.92

GC content: 38.28

Gene sequence:

>930_bases
CTGATTTTATTGATTATGAATACAGACGATTTCCAGTTTTTTATTCGCGTGGCTGACTGTGGTTCACTGAGCAAAGCAGC
TCAAGAAGCCAATATTTCGGTGTCTGTTGCCAGCCAACGGATTCAAAGGCTTGAAAGCACATTAAAATTGCGCTTGTTTT
ATCGAACAACTCGAAAAATCACCTTAACCGATGAAGGAAGAAAATTACTCGATTTTGGTCGATACTGGCTTGACGATTTT
ATAACGTTAAAAGATACGCTTACAGCTCGCGATAAACCGCTCACAGGAAATTTAAGAGTCACAACATCCGCAACATTTGG
CATTAAGATTTTAACCAAGGTGATTGCCAATTTTGCTTTGCTTCACCCTACTCTGAATATTTACTTGGATTTAAATGATC
AAAATATCGATCTGATTGAACATGGAATGGATTTAGCCATTAGAATTGGCAAACTCAATGATTCCAGTTTGGTTGCCAAA
CCTTTGATGACAAATCATCGTCTAATCTGTGCAGCTCCCTCATACCTAAACCAATACGGAATGCCACATAGTATTGAAGA
TTTGCAACAGCATCGATGTATTTTACAACAACATCAAAATGGCCTAACTGATCACTGGCAATTGATTGATCAGAAAAGAA
CGGTTGTTGATGTTCACTTGAAAGCTTACTTTACAACAAACTCTGGCGAGGCAATTCGTCAGGCAGCACTTCAAGGTTTG
GGTATTTCTAATCACTCTCGATGGCATGTCGCGGATGATTTAGAACAAGGGCATCTGATTGAACTCCTTCCTGAGTATCA
AATTGAACCGACAACCATTTATGCGGTATTTCCAAACCGTCAATTTCGCCAGCCGAAAACTGAATTATTTTTAAGTTATT
TACAGAATTACTTTAAACACGAATATCCATGGAATATCAGAAAAAACTAG

Upstream 100 bases:

>100_bases
CGATATTTCATGATGGCTCTCAATATGAACGATTCATTACATCATCCAAGATAGGTCTATAAGATTTTTTAAAAAAGCAG
GATAATCAGAAAAGACTATT

Downstream 100 bases:

>100_bases
ATAAATTAATCATTTAAACTATAAATAAATCCACATTTCCATCTAAGTAATTGATCATAATGTGCTTTAGCTTAAGGTCG
GTATGTTAGAGTCATTGTGT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MILLIMNTDDFQFFIRVADCGSLSKAAQEANISVSVASQRIQRLESTLKLRLFYRTTRKITLTDEGRKLLDFGRYWLDDF
ITLKDTLTARDKPLTGNLRVTTSATFGIKILTKVIANFALLHPTLNIYLDLNDQNIDLIEHGMDLAIRIGKLNDSSLVAK
PLMTNHRLICAAPSYLNQYGMPHSIEDLQQHRCILQQHQNGLTDHWQLIDQKRTVVDVHLKAYFTTNSGEAIRQAALQGL
GISNHSRWHVADDLEQGHLIELLPEYQIEPTTIYAVFPNRQFRQPKTELFLSYLQNYFKHEYPWNIRKN

Sequences:

>Translated_309_residues
MILLIMNTDDFQFFIRVADCGSLSKAAQEANISVSVASQRIQRLESTLKLRLFYRTTRKITLTDEGRKLLDFGRYWLDDF
ITLKDTLTARDKPLTGNLRVTTSATFGIKILTKVIANFALLHPTLNIYLDLNDQNIDLIEHGMDLAIRIGKLNDSSLVAK
PLMTNHRLICAAPSYLNQYGMPHSIEDLQQHRCILQQHQNGLTDHWQLIDQKRTVVDVHLKAYFTTNSGEAIRQAALQGL
GISNHSRWHVADDLEQGHLIELLPEYQIEPTTIYAVFPNRQFRQPKTELFLSYLQNYFKHEYPWNIRKN
>Mature_309_residues
MILLIMNTDDFQFFIRVADCGSLSKAAQEANISVSVASQRIQRLESTLKLRLFYRTTRKITLTDEGRKLLDFGRYWLDDF
ITLKDTLTARDKPLTGNLRVTTSATFGIKILTKVIANFALLHPTLNIYLDLNDQNIDLIEHGMDLAIRIGKLNDSSLVAK
PLMTNHRLICAAPSYLNQYGMPHSIEDLQQHRCILQQHQNGLTDHWQLIDQKRTVVDVHLKAYFTTNSGEAIRQAALQGL
GISNHSRWHVADDLEQGHLIELLPEYQIEPTTIYAVFPNRQFRQPKTELFLSYLQNYFKHEYPWNIRKN

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=295, Percent_Identity=31.864406779661, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI1789440, Length=291, Percent_Identity=30.2405498281787, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI145693193, Length=292, Percent_Identity=30.8219178082192, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1786401, Length=306, Percent_Identity=29.0849673202614, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1789639, Length=288, Percent_Identity=30.2083333333333, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1787128, Length=292, Percent_Identity=26.7123287671233, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1787589, Length=288, Percent_Identity=26.0416666666667, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1788706, Length=293, Percent_Identity=25.2559726962457, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788481, Length=263, Percent_Identity=25.8555133079848, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786448, Length=285, Percent_Identity=25.2631578947368, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789173, Length=288, Percent_Identity=23.6111111111111, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI157672245, Length=163, Percent_Identity=26.9938650306748, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790208, Length=172, Percent_Identity=28.4883720930233, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35693; Mature: 35693

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILLIMNTDDFQFFIRVADCGSLSKAAQEANISVSVASQRIQRLESTLKLRLFYRTTRKI
CEEEEEECCCEEEEEEEECCCCHHHHHHHCCCEEHHHHHHHHHHHHHHEEEEEEEECEEE
TLTDEGRKLLDFGRYWLDDFITLKDTLTARDKPLTGNLRVTTSATFGIKILTKVIANFAL
EEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHH
LHPTLNIYLDLNDQNIDLIEHGMDLAIRIGKLNDSSLVAKPLMTNHRLICAAPSYLNQYG
CCCEEEEEEEECCCCCHHHHCCCEEEEEEECCCCCCCEECHHCCCCEEEEECCHHHHHCC
MPHSIEDLQQHRCILQQHQNGLTDHWQLIDQKRTVVDVHLKAYFTTNSGEAIRQAALQGL
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEEEEEEEEEEECCCCHHHHHHHHHCC
GISNHSRWHVADDLEQGHLIELLPEYQIEPTTIYAVFPNRQFRQPKTELFLSYLQNYFKH
CCCCCCCEEECCCCCCCCEEEECCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCC
EYPWNIRKN
CCCCCCCCC
>Mature Secondary Structure
MILLIMNTDDFQFFIRVADCGSLSKAAQEANISVSVASQRIQRLESTLKLRLFYRTTRKI
CEEEEEECCCEEEEEEEECCCCHHHHHHHCCCEEHHHHHHHHHHHHHHEEEEEEEECEEE
TLTDEGRKLLDFGRYWLDDFITLKDTLTARDKPLTGNLRVTTSATFGIKILTKVIANFAL
EEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHH
LHPTLNIYLDLNDQNIDLIEHGMDLAIRIGKLNDSSLVAKPLMTNHRLICAAPSYLNQYG
CCCEEEEEEEECCCCCHHHHCCCEEEEEEECCCCCCCEECHHCCCCEEEEECCHHHHHCC
MPHSIEDLQQHRCILQQHQNGLTDHWQLIDQKRTVVDVHLKAYFTTNSGEAIRQAALQGL
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEEEEEEEEEEECCCCHHHHHHHHHCC
GISNHSRWHVADDLEQGHLIELLPEYQIEPTTIYAVFPNRQFRQPKTELFLSYLQNYFKH
CCCCCCCEEECCCCCCCCEEEECCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCC
EYPWNIRKN
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]