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The map label for this gene is panB [H]

Identifier: 50086073

GI number: 50086073

Start: 2987780

End: 2988532

Strand: Reverse

Name: panB [H]

Synonym: ACIAD3061

Alternate gene names: 50086073

Gene position: 2988532-2987780 (Counterclockwise)

Preceding gene: 50086074

Following gene: 50086072

Centisome position: 83.05

GC content: 44.22

Gene sequence:

>753_bases
TTGACTTGTTATGATGCAAGTATGGCTAAAGCGATGGAGTTTGCCGAAATTGACTCGATTCTTATTGGGGATTCTTTAGG
GATGGTCATTCAGGGTCACGATTCGACTTTACCTGTTACGGTGGATGATATGGCTTACCATACTGCGGCTGTACGTCGTG
GTAATCAGCATTCGTTTATTATGACTGACTTACCCTTTATGAGTTATGCCACCTTGCCTGATGCCCTGAATAACGCCAAA
AAAGTCATGCAGGCAGGGGCACAGATGATCAAGATTGAAGGTGGTGCCTGGTTAAGCGAATCCATTCGTGTTTTGACACA
AAATGGTATTCCTGTATGTGTACACTTGGGTTTAACACCTCAGTCTGTACATGTTTTTGGTGGATATAAATTACAAGCCA
AAACTCGAGAAGCTGCGGATCAACTGATTGCAGATTGTCAGGCGGTGGTTGATGCGGGAGCCGCAATTTTGTTACTCGAA
TGTGTGCCAGCGCAGTTAGGGCAGGAAATTGCAGAATTATTTCCACAAACGCCTGTCATTGGTATTGGTGCGGGTAAAGA
AACTGATGGACAGGTATTGGTTGCTCAAGACATGCTGGGACTCAGCTTTGGCAAAGTAGCACGTTTTGTCCGTAACTTTA
TGAAAGAACAAGCTGGAGAGACTGCAATTGTAGATGCATTTAAGGCATATCATGCTGCTGTACAAGACCAGTCATTTCCT
GCACGAGAACATACATTTCAGGTAGAGCTGTAA

Upstream 100 bases:

>100_bases
AAATAACGCGCTGTTGTGGGTAGCGTTGATAGAGGACATCACGATGATTAGTCTAAGTGACTTAAGAAAGTTTAAAACTG
AAGGACGCAAATTTTCCTGC

Downstream 100 bases:

>100_bases
TTTATGAAAACCGAAACCACGATTCAGGGATTAACCGCGTCACTCAATCCTGCACGTACAACACGCAAGATTATTGGTTT
TGTGCCAACGATGGGTAATT

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT [H]

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MTCYDASMAKAMEFAEIDSILIGDSLGMVIQGHDSTLPVTVDDMAYHTAAVRRGNQHSFIMTDLPFMSYATLPDALNNAK
KVMQAGAQMIKIEGGAWLSESIRVLTQNGIPVCVHLGLTPQSVHVFGGYKLQAKTREAADQLIADCQAVVDAGAAILLLE
CVPAQLGQEIAELFPQTPVIGIGAGKETDGQVLVAQDMLGLSFGKVARFVRNFMKEQAGETAIVDAFKAYHAAVQDQSFP
AREHTFQVEL

Sequences:

>Translated_250_residues
MTCYDASMAKAMEFAEIDSILIGDSLGMVIQGHDSTLPVTVDDMAYHTAAVRRGNQHSFIMTDLPFMSYATLPDALNNAK
KVMQAGAQMIKIEGGAWLSESIRVLTQNGIPVCVHLGLTPQSVHVFGGYKLQAKTREAADQLIADCQAVVDAGAAILLLE
CVPAQLGQEIAELFPQTPVIGIGAGKETDGQVLVAQDMLGLSFGKVARFVRNFMKEQAGETAIVDAFKAYHAAVQDQSFP
AREHTFQVEL
>Mature_249_residues
TCYDASMAKAMEFAEIDSILIGDSLGMVIQGHDSTLPVTVDDMAYHTAAVRRGNQHSFIMTDLPFMSYATLPDALNNAKK
VMQAGAQMIKIEGGAWLSESIRVLTQNGIPVCVHLGLTPQSVHVFGGYKLQAKTREAADQLIADCQAVVDAGAAILLLEC
VPAQLGQEIAELFPQTPVIGIGAGKETDGQVLVAQDMLGLSFGKVARFVRNFMKEQAGETAIVDAFKAYHAAVQDQSFPA
REHTFQVEL

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate [H]

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family [H]

Homologues:

Organism=Escherichia coli, GI1786326, Length=247, Percent_Identity=52.2267206477733, Blast_Score=266, Evalue=9e-73,
Organism=Saccharomyces cerevisiae, GI6319653, Length=259, Percent_Identity=37.0656370656371, Blast_Score=159, Evalue=4e-40,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003700
- InterPro:   IPR015813 [H]

Pfam domain/function: PF02548 Pantoate_transf [H]

EC number: =2.1.2.11 [H]

Molecular weight: Translated: 26900; Mature: 26769

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTCYDASMAKAMEFAEIDSILIGDSLGMVIQGHDSTLPVTVDDMAYHTAAVRRGNQHSFI
CCCCCHHHHHHHHHHHHHHEEECCCCCEEEECCCCCCCEEEHHHHHHHHHHHCCCCCCEE
MTDLPFMSYATLPDALNNAKKVMQAGAQMIKIEGGAWLSESIRVLTQNGIPVCVHLGLTP
EECCCHHHHHHCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHEEECCCCEEEEEECCCC
QSVHVFGGYKLQAKTREAADQLIADCQAVVDAGAAILLLECVPAQLGQEIAELFPQTPVI
CEEEEECCEEEEHHHHHHHHHHHHHHHHHHHCCHHEEHHHHHHHHHHHHHHHHCCCCCEE
GIGAGKETDGQVLVAQDMLGLSFGKVARFVRNFMKEQAGETAIVDAFKAYHAAVQDQSFP
EECCCCCCCCCEEEEHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCC
AREHTFQVEL
CCCCEEEEEC
>Mature Secondary Structure 
TCYDASMAKAMEFAEIDSILIGDSLGMVIQGHDSTLPVTVDDMAYHTAAVRRGNQHSFI
CCCCHHHHHHHHHHHHHHEEECCCCCEEEECCCCCCCEEEHHHHHHHHHHHCCCCCCEE
MTDLPFMSYATLPDALNNAKKVMQAGAQMIKIEGGAWLSESIRVLTQNGIPVCVHLGLTP
EECCCHHHHHHCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHEEECCCCEEEEEECCCC
QSVHVFGGYKLQAKTREAADQLIADCQAVVDAGAAILLLECVPAQLGQEIAELFPQTPVI
CEEEEECCEEEEHHHHHHHHHHHHHHHHHHHCCHHEEHHHHHHHHHHHHHHHHCCCCCEE
GIGAGKETDGQVLVAQDMLGLSFGKVARFVRNFMKEQAGETAIVDAFKAYHAAVQDQSFP
EECCCCCCCCCEEEEHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCC
AREHTFQVEL
CCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA