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The map label for this gene is mesJ

Identifier: 50086047

GI number: 50086047

Start: 2963347

End: 2964726

Strand: Reverse

Name: mesJ

Synonym: ACIAD3034

Alternate gene names: 50086047

Gene position: 2964726-2963347 (Counterclockwise)

Preceding gene: 50086048

Following gene: 50086046

Centisome position: 82.39

GC content: 41.23

Gene sequence:

>1380_bases
ATGCGAAGCGCGTTACCAACGTTTGATGAAATTTGGCAGCGAAAATTTAGGCATCGCCTCTTAATACAGGCTCAGCAATT
TCCTGCTGATACCGTATTCTTAATTGGATGCAGCGGTGGCATGGATTCCATGTTATTGCTGCATTTGATGTCGGAACTGT
TTCCTCATAAAGTACGTGCGATTTATATCGATCACCAGCTACAATCCAGTAGTCGGGCATGGGGAGTGTTTGTTCAAAAT
TTTGCACAACAGTCTCATATTCCCTTCACTATCCAGCCGGTTATTGTTGATACGGGTAATCTGGAAAATCAGGCCAGAGA
AGCACGATATGCTGCGTTTGAATCGCATTTAAAATCGAATGAAGTTTTAGTATTGGCCCATCATCAGCAAGATCAGACTG
AAACCGTTTTACTCAGGTTACTCTCTGGCAGTGGTGTGAAAGGGCTGGGTGCCATGAAAGAAATCGAACAGAAAAAAAAT
ATATGCTGCTGGCGGCCTATGTTATCTGTTTCAAGACAACAGATTGAACACTGGGTAGAACATCTTAAAATACCTTATAT
CCAAGATTTGACTAACTTTGATACCACTTATGATCGGGCATGGTGCCGTGAAACTGTTTGGCCTGTCCTTCAAAAGCGTT
TTCCCAAAATGCAGGAAGCGATCAGTCGAACCAGTATTTTGATGCAGGATGCTGATGAAATTCTACATGAGGTTTTGCAG
CAAGATTTGAATCAGTGTGGTGACCTTAATCATCTAGATCTTAGTCGATTACAACAACTTTCTCTGGCAAGACAACGACA
GCTTTTATCTTTCTGGATGAAAGGGAAGGCGATTTATCGGCCAGCATTTGAAATGGTGGAACGAGTACAACAGGAAGTCA
TTTGTGCAAAAGCTGATGCAAAAGCGGCATTGCACTGGAATCATCATTACTACGTACGCTACCAAAATATTTTATATCGT
GTTGAAAAAAATAAATATTTGCAAAAAGATTTAGTTTCTAATGTAGTGAGTACTATTGAGTTGCAGATGGATGCATCCTT
GCAGCTTGCCTCTGGGGTATTTCAGATTCAGCCCATGCAAATGGGCTTATCACCACAATTATTCGAACAGCCTTTGCAGC
TACTTCCCAGACAAGGCGGAGAAAAAATTCATCTGTACGGACGTGTAGGACATTGGCCATTAAAAAAAGCCATTCAGGAG
GCACAAATTTTGCCTTGGTTGCGTCATACAATTCAAATATTGGCGTTGGATAATGTTATGCTAGGCGTTTTTACTCCCAA
AGGTTTTTGGCTGGCACAATCATCGTATTGTGAAGCTGGGGGATGGCAACCTAATTTAATTTCTGATGTAAATTATTTGC
GAGTTGAGCAGAATTCATGA

Upstream 100 bases:

>100_bases
GCGAAGGTGCGCATTTACATCCAGAACAGGTTATGCAAAATCTTCAAGTCGTGTTAAAACAGGCGCTAGATGAATTGCTT
GCTATGGATGCAGATGAACG

Downstream 100 bases:

>100_bases
GTGCGACATTAAAATCAAATATCACATTTATTGGTGGTGGTAACATGGCGCAAGCCCTCATCGGAGGGCTGATTGCGCGT
GGATTACCTTCAACTCGGAT

Product: cell cycle protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MRSALPTFDEIWQRKFRHRLLIQAQQFPADTVFLIGCSGGMDSMLLLHLMSELFPHKVRAIYIDHQLQSSSRAWGVFVQN
FAQQSHIPFTIQPVIVDTGNLENQAREARYAAFESHLKSNEVLVLAHHQQDQTETVLLRLLSGSGVKGLGAMKEIEQKKN
ICCWRPMLSVSRQQIEHWVEHLKIPYIQDLTNFDTTYDRAWCRETVWPVLQKRFPKMQEAISRTSILMQDADEILHEVLQ
QDLNQCGDLNHLDLSRLQQLSLARQRQLLSFWMKGKAIYRPAFEMVERVQQEVICAKADAKAALHWNHHYYVRYQNILYR
VEKNKYLQKDLVSNVVSTIELQMDASLQLASGVFQIQPMQMGLSPQLFEQPLQLLPRQGGEKIHLYGRVGHWPLKKAIQE
AQILPWLRHTIQILALDNVMLGVFTPKGFWLAQSSYCEAGGWQPNLISDVNYLRVEQNS

Sequences:

>Translated_459_residues
MRSALPTFDEIWQRKFRHRLLIQAQQFPADTVFLIGCSGGMDSMLLLHLMSELFPHKVRAIYIDHQLQSSSRAWGVFVQN
FAQQSHIPFTIQPVIVDTGNLENQAREARYAAFESHLKSNEVLVLAHHQQDQTETVLLRLLSGSGVKGLGAMKEIEQKKN
ICCWRPMLSVSRQQIEHWVEHLKIPYIQDLTNFDTTYDRAWCRETVWPVLQKRFPKMQEAISRTSILMQDADEILHEVLQ
QDLNQCGDLNHLDLSRLQQLSLARQRQLLSFWMKGKAIYRPAFEMVERVQQEVICAKADAKAALHWNHHYYVRYQNILYR
VEKNKYLQKDLVSNVVSTIELQMDASLQLASGVFQIQPMQMGLSPQLFEQPLQLLPRQGGEKIHLYGRVGHWPLKKAIQE
AQILPWLRHTIQILALDNVMLGVFTPKGFWLAQSSYCEAGGWQPNLISDVNYLRVEQNS
>Mature_459_residues
MRSALPTFDEIWQRKFRHRLLIQAQQFPADTVFLIGCSGGMDSMLLLHLMSELFPHKVRAIYIDHQLQSSSRAWGVFVQN
FAQQSHIPFTIQPVIVDTGNLENQAREARYAAFESHLKSNEVLVLAHHQQDQTETVLLRLLSGSGVKGLGAMKEIEQKKN
ICCWRPMLSVSRQQIEHWVEHLKIPYIQDLTNFDTTYDRAWCRETVWPVLQKRFPKMQEAISRTSILMQDADEILHEVLQ
QDLNQCGDLNHLDLSRLQQLSLARQRQLLSFWMKGKAIYRPAFEMVERVQQEVICAKADAKAALHWNHHYYVRYQNILYR
VEKNKYLQKDLVSNVVSTIELQMDASLQLASGVFQIQPMQMGLSPQLFEQPLQLLPRQGGEKIHLYGRVGHWPLKKAIQE
AQILPWLRHTIQILALDNVMLGVFTPKGFWLAQSSYCEAGGWQPNLISDVNYLRVEQNS

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=403, Percent_Identity=32.258064516129, Blast_Score=171, Evalue=7e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_ACIAD (Q6F880)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_047557.1
- ProteinModelPortal:   Q6F880
- SMR:   Q6F880
- STRING:   Q6F880
- GeneID:   2880583
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD3034
- NMPDR:   fig|62977.3.peg.2750
- eggNOG:   COG0037
- HOGENOM:   HBG289727
- OMA:   KSAVGFF
- PhylomeDB:   Q6F880
- ProtClustDB:   CLSK707746
- BioCyc:   ASP62977:ACIAD3034-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- SMART:   SM00977
- TIGRFAMs:   TIGR02433
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C; SSF56037 B3_4

EC number: NA

Molecular weight: Translated: 53231; Mature: 53231

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSALPTFDEIWQRKFRHRLLIQAQQFPADTVFLIGCSGGMDSMLLLHLMSELFPHKVRA
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEE
IYIDHQLQSSSRAWGVFVQNFAQQSHIPFTIQPVIVDTGNLENQAREARYAAFESHLKSN
EEEECHHCCCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCC
EVLVLAHHQQDQTETVLLRLLSGSGVKGLGAMKEIEQKKNICCWRPMLSVSRQQIEHWVE
CEEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHH
HLKIPYIQDLTNFDTTYDRAWCRETVWPVLQKRFPKMQEAISRTSILMQDADEILHEVLQ
HCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHH
QDLNQCGDLNHLDLSRLQQLSLARQRQLLSFWMKGKAIYRPAFEMVERVQQEVICAKADA
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC
KAALHWNHHYYVRYQNILYRVEKNKYLQKDLVSNVVSTIELQMDASLQLASGVFQIQPMQ
CEEEEECCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCEEEEHHH
MGLSPQLFEQPLQLLPRQGGEKIHLYGRVGHWPLKKAIQEAQILPWLRHTIQILALDNVM
CCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEE
LGVFTPKGFWLAQSSYCEAGGWQPNLISDVNYLRVEQNS
EEEECCCCEEEECCCCCCCCCCCCCHHCCCCEEEEECCC
>Mature Secondary Structure
MRSALPTFDEIWQRKFRHRLLIQAQQFPADTVFLIGCSGGMDSMLLLHLMSELFPHKVRA
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEE
IYIDHQLQSSSRAWGVFVQNFAQQSHIPFTIQPVIVDTGNLENQAREARYAAFESHLKSN
EEEECHHCCCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCC
EVLVLAHHQQDQTETVLLRLLSGSGVKGLGAMKEIEQKKNICCWRPMLSVSRQQIEHWVE
CEEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHH
HLKIPYIQDLTNFDTTYDRAWCRETVWPVLQKRFPKMQEAISRTSILMQDADEILHEVLQ
HCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHH
QDLNQCGDLNHLDLSRLQQLSLARQRQLLSFWMKGKAIYRPAFEMVERVQQEVICAKADA
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC
KAALHWNHHYYVRYQNILYRVEKNKYLQKDLVSNVVSTIELQMDASLQLASGVFQIQPMQ
CEEEEECCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCEEEEHHH
MGLSPQLFEQPLQLLPRQGGEKIHLYGRVGHWPLKKAIQEAQILPWLRHTIQILALDNVM
CCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEE
LGVFTPKGFWLAQSSYCEAGGWQPNLISDVNYLRVEQNS
EEEECCCCEEEECCCCCCCCCCCCCHHCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA