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The map label for this gene is cysW [H]

Identifier: 50085668

GI number: 50085668

Start: 2551960

End: 2552883

Strand: Direct

Name: cysW [H]

Synonym: ACIAD2595

Alternate gene names: 50085668

Gene position: 2551960-2552883 (Clockwise)

Preceding gene: 50085667

Following gene: 50085669

Centisome position: 70.91

GC content: 42.32

Gene sequence:

>924_bases
ATGGGCGAGCCGTCGCACAGGGAGAACGGTGAAATGAGCTTAATTACCCATAACAGTGAATTAGCTGAAAAGTTACAAAG
TCGAGATGCGACACGTGAGCCAACTTGGGTGAGATACCTTTTACTCACCATTGCACTGCTTTTCTTTTTGAGCTGCCTCA
TTCTGCCTTTAGTGTTGGTCTTTGTAGAAGCATTTAGACAAGGTATTGATGTTTATATTAATGCATTAACTAACTCAGAT
ACGTTATCAGCTGTAAAACTTACACTACTTACCGCCGTTATTGCAGTACCACTTAACGTCGTATTTGGAGTTGCAGCAGC
CTGGGCAGTTGCCAAGTTTCAGTTCCGTGGTAAATCTTTTCTGACCACGCTGATTGATTTGCCATTTTCAGTTTCACCTG
TCATCGCAGGTTTAATGTTAGTGCTGATTTTTGGTATGCAAGGTTGGTTTGGTCCATGGTTGATGGATCATGACATTAAG
ATTTTATACGCCGTACCTGCGATTGTAATTGCAACCATTTTTATTACCGTACCTTTTGTGGCACGTGAGTTGATTCCGCT
CATGGAAGCGCAAGGAATTGAAGAAGAAGAAGCTGCTATTGTATTGGGTGCTTCTGGATGGCAAACCTTTTGGAAGGTTA
CCTTACCCAATATTAAATGGGGATTGATTTATGGCGTGATTCTATGTAATGCACGTGCCATGGGTGAGTTTGGCGCAGTT
TCGGTGGTATCAGGTCATATTCGTGGTGAAACCAATACTTTACCACTGCATGTCGAGATTCTGTATAACGAATACACGTT
TAGCGCCGCCTTTGCAGTATCTTCCCTTCTGGCTTTACTGGCAATTGTAACGTTGATCTTGAAAACATGGGTCGAAGCGC
GCCAAGAAAAACAGAATAAACATCAGAATTCAACCACATCTTAA

Upstream 100 bases:

>100_bases
CGCGTTTAGAAGAATATGACTATGCCGCCGCAACGACCATTGCTGTCATTATGCTGGTGATTTCTTTTGTAATTCTACTT
TTCATCAATTTATTGCAAGC

Downstream 100 bases:

>100_bases
GGAATAGCCATGAGTATTCAAGTTAAAAATATTGAAAAACATTTTGGTGCATTCCATGCACTTAAGAATATTTCTCTGGA
CTTTCCAGAGGGTCAACTGG

Product: sulfate ABC transporter

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MGEPSHRENGEMSLITHNSELAEKLQSRDATREPTWVRYLLLTIALLFFLSCLILPLVLVFVEAFRQGIDVYINALTNSD
TLSAVKLTLLTAVIAVPLNVVFGVAAAWAVAKFQFRGKSFLTTLIDLPFSVSPVIAGLMLVLIFGMQGWFGPWLMDHDIK
ILYAVPAIVIATIFITVPFVARELIPLMEAQGIEEEEAAIVLGASGWQTFWKVTLPNIKWGLIYGVILCNARAMGEFGAV
SVVSGHIRGETNTLPLHVEILYNEYTFSAAFAVSSLLALLAIVTLILKTWVEARQEKQNKHQNSTTS

Sequences:

>Translated_307_residues
MGEPSHRENGEMSLITHNSELAEKLQSRDATREPTWVRYLLLTIALLFFLSCLILPLVLVFVEAFRQGIDVYINALTNSD
TLSAVKLTLLTAVIAVPLNVVFGVAAAWAVAKFQFRGKSFLTTLIDLPFSVSPVIAGLMLVLIFGMQGWFGPWLMDHDIK
ILYAVPAIVIATIFITVPFVARELIPLMEAQGIEEEEAAIVLGASGWQTFWKVTLPNIKWGLIYGVILCNARAMGEFGAV
SVVSGHIRGETNTLPLHVEILYNEYTFSAAFAVSSLLALLAIVTLILKTWVEARQEKQNKHQNSTTS
>Mature_306_residues
GEPSHRENGEMSLITHNSELAEKLQSRDATREPTWVRYLLLTIALLFFLSCLILPLVLVFVEAFRQGIDVYINALTNSDT
LSAVKLTLLTAVIAVPLNVVFGVAAAWAVAKFQFRGKSFLTTLIDLPFSVSPVIAGLMLVLIFGMQGWFGPWLMDHDIKI
LYAVPAIVIATIFITVPFVARELIPLMEAQGIEEEEAAIVLGASGWQTFWKVTLPNIKWGLIYGVILCNARAMGEFGAVS
VVSGHIRGETNTLPLHVEILYNEYTFSAAFAVSSLLALLAIVTLILKTWVEARQEKQNKHQNSTTS

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4208

COG function: function code P; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87082099, Length=278, Percent_Identity=49.6402877697842, Blast_Score=286, Evalue=1e-78,
Organism=Escherichia coli, GI1788764, Length=206, Percent_Identity=39.8058252427184, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1786980, Length=221, Percent_Identity=30.316742081448, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1787369, Length=159, Percent_Identity=29.559748427673, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33875; Mature: 33744

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00027 HOMEOBOX_1 ; PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEPSHRENGEMSLITHNSELAEKLQSRDATREPTWVRYLLLTIALLFFLSCLILPLVLV
CCCCCCCCCCCEEEEECCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FVEAFRQGIDVYINALTNSDTLSAVKLTLLTAVIAVPLNVVFGVAAAWAVAKFQFRGKSF
HHHHHHCCHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LTTLIDLPFSVSPVIAGLMLVLIFGMQGWFGPWLMDHDIKILYAVPAIVIATIFITVPFV
HHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHH
ARELIPLMEAQGIEEEEAAIVLGASGWQTFWKVTLPNIKWGLIYGVILCNARAMGEFGAV
HHHHHHHHHHCCCCCCCCEEEEECCCCCCEEEEECCCCHHHHHHHHHHHCCHHCCCCCCH
SVVSGHIRGETNTLPLHVEILYNEYTFSAAFAVSSLLALLAIVTLILKTWVEARQEKQNK
HHEECCCCCCCCCEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HQNSTTS
CCCCCCC
>Mature Secondary Structure 
GEPSHRENGEMSLITHNSELAEKLQSRDATREPTWVRYLLLTIALLFFLSCLILPLVLV
CCCCCCCCCCEEEEECCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FVEAFRQGIDVYINALTNSDTLSAVKLTLLTAVIAVPLNVVFGVAAAWAVAKFQFRGKSF
HHHHHHCCHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LTTLIDLPFSVSPVIAGLMLVLIFGMQGWFGPWLMDHDIKILYAVPAIVIATIFITVPFV
HHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHH
ARELIPLMEAQGIEEEEAAIVLGASGWQTFWKVTLPNIKWGLIYGVILCNARAMGEFGAV
HHHHHHHHHHCCCCCCCCEEEEECCCCCCEEEEECCCCHHHHHHHHHHHCCHHCCCCCCH
SVVSGHIRGETNTLPLHVEILYNEYTFSAAFAVSSLLALLAIVTLILKTWVEARQEKQNK
HHEECCCCCCCCCEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HQNSTTS
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]