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The map label for this gene is cysT [H]

Identifier: 50085667

GI number: 50085667

Start: 2551163

End: 2551996

Strand: Direct

Name: cysT [H]

Synonym: ACIAD2594

Alternate gene names: 50085667

Gene position: 2551163-2551996 (Clockwise)

Preceding gene: 50085666

Following gene: 50085668

Centisome position: 70.89

GC content: 43.76

Gene sequence:

>834_bases
ATGTCGCAGCGATCCCGAGTGCTGCCAGGTTTTGGTCTATCTTTAGGCTTTACCCTCGCGTATTTATCCCTGATCGTCTT
GATTCCTTTATCAGCAGTTTTTATTAAATCCTTAGGGGTGGGCTGGGAAGGTCTTTGGGAGATCTTAAGTTCTCAACGAA
TTCTTAAATCGTTGCAACTGAGTTTTACCACAGCGCTAATTGCAGCATTGATTAATGTTGTTTTTGGATTATTGCTGGCG
TGGTGTCTGGTTCGTTACACCTTTCCAGGTAAACGTATTGTTGATGCTTTGGTGGATCTACCATTTGCCTTACCCACTGC
TGTGGCAGGTATTGCCCTGACCTCACTCTATGCATCGACAGGATGGATTGGTCAATACCTCGAACCTTTGGGTATTAAAG
TGGCTTATACCCCGCTTGGGATTACGCTGGCCTTAATTTTTATTGGACTACCTTTTGTGGTTCGAACTGTTCAGCCTGTT
TTGAGTGATATTGAAACTGAACTTGAAGAAGCGGCTTCAGCACTTGGCGCAAATCGCTTTCAGATCGTTAGAAAAATCAT
TTTACCGATTCTGTTTCCAGCACTTCTAACAGGTTTTGCACTGGCATTTGCACGCGGTGTGGGTGAATATGGTTCAGTTA
TTTTTATTGCGGGCAACCAGCCATTCAAAACTGAAATTGCACCGCTCATGATTATTTCGCGTTTAGAAGAATATGACTAT
GCCGCCGCAACGACCATTGCTGTCATTATGCTGGTGATTTCTTTTGTAATTCTACTTTTCATCAATTTATTGCAAGCATG
GGCGAGCCGTCGCACAGGGAGAACGGTGAAATGA

Upstream 100 bases:

>100_bases
TGTTCAAGTTTGCACTTTAGTTTCAGTTCACATTATTTCGTTTTTATTCTATTGATTCGTTTGCTGCTTTTGGCAAACAC
GCTCTCTTTTAGGAGTTATC

Downstream 100 bases:

>100_bases
GCTTAATTACCCATAACAGTGAATTAGCTGAAAAGTTACAAAGTCGAGATGCGACACGTGAGCCAACTTGGGTGAGATAC
CTTTTACTCACCATTGCACT

Product: sulfate ABC transporter

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MSQRSRVLPGFGLSLGFTLAYLSLIVLIPLSAVFIKSLGVGWEGLWEILSSQRILKSLQLSFTTALIAALINVVFGLLLA
WCLVRYTFPGKRIVDALVDLPFALPTAVAGIALTSLYASTGWIGQYLEPLGIKVAYTPLGITLALIFIGLPFVVRTVQPV
LSDIETELEEAASALGANRFQIVRKIILPILFPALLTGFALAFARGVGEYGSVIFIAGNQPFKTEIAPLMIISRLEEYDY
AAATTIAVIMLVISFVILLFINLLQAWASRRTGRTVK

Sequences:

>Translated_277_residues
MSQRSRVLPGFGLSLGFTLAYLSLIVLIPLSAVFIKSLGVGWEGLWEILSSQRILKSLQLSFTTALIAALINVVFGLLLA
WCLVRYTFPGKRIVDALVDLPFALPTAVAGIALTSLYASTGWIGQYLEPLGIKVAYTPLGITLALIFIGLPFVVRTVQPV
LSDIETELEEAASALGANRFQIVRKIILPILFPALLTGFALAFARGVGEYGSVIFIAGNQPFKTEIAPLMIISRLEEYDY
AAATTIAVIMLVISFVILLFINLLQAWASRRTGRTVK
>Mature_276_residues
SQRSRVLPGFGLSLGFTLAYLSLIVLIPLSAVFIKSLGVGWEGLWEILSSQRILKSLQLSFTTALIAALINVVFGLLLAW
CLVRYTFPGKRIVDALVDLPFALPTAVAGIALTSLYASTGWIGQYLEPLGIKVAYTPLGITLALIFIGLPFVVRTVQPVL
SDIETELEEAASALGANRFQIVRKIILPILFPALLTGFALAFARGVGEYGSVIFIAGNQPFKTEIAPLMIISRLEEYDYA
AATTIAVIMLVISFVILLFINLLQAWASRRTGRTVK

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=266, Percent_Identity=50.7518796992481, Blast_Score=243, Evalue=1e-65,
Organism=Escherichia coli, GI87082099, Length=243, Percent_Identity=32.9218106995885, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1786980, Length=221, Percent_Identity=32.579185520362, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1787368, Length=244, Percent_Identity=30.327868852459, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1786254, Length=221, Percent_Identity=26.6968325791855, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1787369, Length=223, Percent_Identity=25.1121076233184, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30090; Mature: 29959

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQRSRVLPGFGLSLGFTLAYLSLIVLIPLSAVFIKSLGVGWEGLWEILSSQRILKSLQL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
SFTTALIAALINVVFGLLLAWCLVRYTFPGKRIVDALVDLPFALPTAVAGIALTSLYAST
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
GWIGQYLEPLGIKVAYTPLGITLALIFIGLPFVVRTVQPVLSDIETELEEAASALGANRF
CHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
QIVRKIILPILFPALLTGFALAFARGVGEYGSVIFIAGNQPFKTEIAPLMIISRLEEYDY
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
AAATTIAVIMLVISFVILLFINLLQAWASRRTGRTVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SQRSRVLPGFGLSLGFTLAYLSLIVLIPLSAVFIKSLGVGWEGLWEILSSQRILKSLQL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
SFTTALIAALINVVFGLLLAWCLVRYTFPGKRIVDALVDLPFALPTAVAGIALTSLYAST
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
GWIGQYLEPLGIKVAYTPLGITLALIFIGLPFVVRTVQPVLSDIETELEEAASALGANRF
CHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
QIVRKIILPILFPALLTGFALAFARGVGEYGSVIFIAGNQPFKTEIAPLMIISRLEEYDY
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
AAATTIAVIMLVISFVILLFINLLQAWASRRTGRTVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]