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The map label for this gene is nadB [H]

Identifier: 50085661

GI number: 50085661

Start: 2545135

End: 2546769

Strand: Direct

Name: nadB [H]

Synonym: ACIAD2587

Alternate gene names: 50085661

Gene position: 2545135-2546769 (Clockwise)

Preceding gene: 50085649

Following gene: 50085665

Centisome position: 70.73

GC content: 42.94

Gene sequence:

>1635_bases
ATGTCTAATTTAGATTCCACTCATCATTTTGATGTGGTGATTGTTGGAAGTGGTGGGGCTGGTCTGAGTCTTGCTCTTTC
ATTGCCAAGCCATTATAAAATCGCAATATTAGCGAAATCTTCCCTTACTGAAGCAAGCACCTTTTATGCACAAGGCGGTG
TTGCGGCGGTTTTGGATAAAACCGACTCTATTCAACAGCATATTGATGACACGATGATTGCAGGTGCTCATTTATGTGAG
CTTGATGCGGTCAAACAAACCGTGGAAGGTGGTCGTCCATCAGTCAATTTTTTATTGAAGCATGGTGTGGAATTCACACT
TGATGAAGATGATCAATTACATTTGACACGTGAGGGTGGTCACTCGCAGCGACGTATTATTCATGCAGCAGATGCTACGG
GAAAAGCAATTTCAACCACATTGGTGCAACGTGCCAAAGAAGCATCCAATCTGACGATTTTTGAAAATTTTATTGCAATT
GATATCATTACATTACATAAACTAGGCCATATCCAGCAAGATAATCGTGCTGTCGGTTTATATGTACTGGATGAACAAAA
TGAAAAAGTCCACACCTTCTTAGCTCCTTTTATCGCTCTGGCTTGTGGCGGCGCGATGAAAGCTTATCTATATACATCAA
ATCCTGATATTGCAACAGGTGATGGTATTGCGATGGCCTATCGCGCGGGTTGCCGAGTCGCAAATATGGAATTTAATCAA
TTCCATCCGACTTGTTTATATCACCCACAAGCGCGTTCATTTTTAATTACCGAAGCCATGCGTGGCGAAGGTGCTTACTT
GCGCTTGCCAGATGGCGAAAGATTTATGCTCCGCTTTGATGAGCGGGCAGAACTTGCACCACGCGACATTGTAGCTCGTG
CAATTGATTATGAAATCAAACGCCTAGGCATCCGCCATGTGTGGCTAGATATTACACACAAATCTGCTGAATTTATTCAG
CAACATTTTCCAACCCTGTATACACGGCTATTAGAACTTGGCATCGATATCACCAAAGATATGATCCCTGTTGTACCTGC
CGCGCATTATACCTGTGGTGGGGTCGTGGTAAATGCACAGAGCCAGACGGATATCGAAGGCTTATATGCTATCGGTGAAA
CATCCTACACTGGTTTGCATGGTGCAAATCGTATGGCCAGTAACTCTTTATTGGAATGCTTTGTTTATGGCTTGAGTGCC
GCAGAAGATATTCAACAAAAGTTTGAGCAGGATTTTAATTTACCTAACATTCCAGAGTGGGATGACTCTCAAGTTACAGA
TGCCGATGAAGATGTAGTCATTCTACAAAACTGGGATGAATTACGTTCCACCATGTGGAACTATGTGGGGATCGTACGCA
CCACCAAGCGTCTACAACGCGCATTACATCGTATCGAAATGCTAAAGCGTGAAATCACCGAATACTATCAGGATTACCGT
GTCAGCAAGAACTTGATTGAATTGCGCAATTTAGTACTTGTATCAGAGATGATTGTACGTTGTGCCATGCAACGCAAGGA
GTCCCGCGGTTTGCACTATACACTGGACTATCCTGAGCTTGAACAACAACTCAAAAAGACGGTTTTAACACCGCCAAATT
TTGAAGTTAAACAAATTCCGGTCAATACGCTTTAA

Upstream 100 bases:

>100_bases
TATTATTGAAAATTTGACTCATATTTAAGTCTATTGTGTCTTGCCTTTTTCAAAAAAAAGCGGTGTTATTAACAAACTTT
TTTAAAATTAGCTGATGGAC

Downstream 100 bases:

>100_bases
CGGATATACAGGCGCTGTATTTATACCAGTGCCTGTATATGCTCAAGCGCTTCCTGAAAAACAATTTCAGGGGCCTGTGT
CGCATCCAGACGTTTGATAC

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 544; Mature: 543

Protein sequence:

>544_residues
MSNLDSTHHFDVVIVGSGGAGLSLALSLPSHYKIAILAKSSLTEASTFYAQGGVAAVLDKTDSIQQHIDDTMIAGAHLCE
LDAVKQTVEGGRPSVNFLLKHGVEFTLDEDDQLHLTREGGHSQRRIIHAADATGKAISTTLVQRAKEASNLTIFENFIAI
DIITLHKLGHIQQDNRAVGLYVLDEQNEKVHTFLAPFIALACGGAMKAYLYTSNPDIATGDGIAMAYRAGCRVANMEFNQ
FHPTCLYHPQARSFLITEAMRGEGAYLRLPDGERFMLRFDERAELAPRDIVARAIDYEIKRLGIRHVWLDITHKSAEFIQ
QHFPTLYTRLLELGIDITKDMIPVVPAAHYTCGGVVVNAQSQTDIEGLYAIGETSYTGLHGANRMASNSLLECFVYGLSA
AEDIQQKFEQDFNLPNIPEWDDSQVTDADEDVVILQNWDELRSTMWNYVGIVRTTKRLQRALHRIEMLKREITEYYQDYR
VSKNLIELRNLVLVSEMIVRCAMQRKESRGLHYTLDYPELEQQLKKTVLTPPNFEVKQIPVNTL

Sequences:

>Translated_544_residues
MSNLDSTHHFDVVIVGSGGAGLSLALSLPSHYKIAILAKSSLTEASTFYAQGGVAAVLDKTDSIQQHIDDTMIAGAHLCE
LDAVKQTVEGGRPSVNFLLKHGVEFTLDEDDQLHLTREGGHSQRRIIHAADATGKAISTTLVQRAKEASNLTIFENFIAI
DIITLHKLGHIQQDNRAVGLYVLDEQNEKVHTFLAPFIALACGGAMKAYLYTSNPDIATGDGIAMAYRAGCRVANMEFNQ
FHPTCLYHPQARSFLITEAMRGEGAYLRLPDGERFMLRFDERAELAPRDIVARAIDYEIKRLGIRHVWLDITHKSAEFIQ
QHFPTLYTRLLELGIDITKDMIPVVPAAHYTCGGVVVNAQSQTDIEGLYAIGETSYTGLHGANRMASNSLLECFVYGLSA
AEDIQQKFEQDFNLPNIPEWDDSQVTDADEDVVILQNWDELRSTMWNYVGIVRTTKRLQRALHRIEMLKREITEYYQDYR
VSKNLIELRNLVLVSEMIVRCAMQRKESRGLHYTLDYPELEQQLKKTVLTPPNFEVKQIPVNTL
>Mature_543_residues
SNLDSTHHFDVVIVGSGGAGLSLALSLPSHYKIAILAKSSLTEASTFYAQGGVAAVLDKTDSIQQHIDDTMIAGAHLCEL
DAVKQTVEGGRPSVNFLLKHGVEFTLDEDDQLHLTREGGHSQRRIIHAADATGKAISTTLVQRAKEASNLTIFENFIAID
IITLHKLGHIQQDNRAVGLYVLDEQNEKVHTFLAPFIALACGGAMKAYLYTSNPDIATGDGIAMAYRAGCRVANMEFNQF
HPTCLYHPQARSFLITEAMRGEGAYLRLPDGERFMLRFDERAELAPRDIVARAIDYEIKRLGIRHVWLDITHKSAEFIQQ
HFPTLYTRLLELGIDITKDMIPVVPAAHYTCGGVVVNAQSQTDIEGLYAIGETSYTGLHGANRMASNSLLECFVYGLSAA
EDIQQKFEQDFNLPNIPEWDDSQVTDADEDVVILQNWDELRSTMWNYVGIVRTTKRLQRALHRIEMLKREITEYYQDYRV
SKNLIELRNLVLVSEMIVRCAMQRKESRGLHYTLDYPELEQQLKKTVLTPPNFEVKQIPVNTL

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=525, Percent_Identity=29.9047619047619, Blast_Score=204, Evalue=2e-52,
Organism=Escherichia coli, GI1788928, Length=535, Percent_Identity=60, Blast_Score=668, Evalue=0.0,
Organism=Escherichia coli, GI1790597, Length=522, Percent_Identity=31.2260536398467, Blast_Score=215, Evalue=7e-57,
Organism=Escherichia coli, GI1786942, Length=561, Percent_Identity=31.1942959001783, Blast_Score=199, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI17505833, Length=538, Percent_Identity=30.6691449814126, Blast_Score=217, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI17550100, Length=544, Percent_Identity=30.3308823529412, Blast_Score=216, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI71986328, Length=425, Percent_Identity=26.3529411764706, Blast_Score=89, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6322416, Length=593, Percent_Identity=31.0286677908938, Blast_Score=217, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6322701, Length=589, Percent_Identity=29.3718166383701, Blast_Score=213, Evalue=5e-56,
Organism=Saccharomyces cerevisiae, GI6320788, Length=481, Percent_Identity=27.6507276507277, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI17137288, Length=562, Percent_Identity=30.0711743772242, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24655642, Length=562, Percent_Identity=30.0711743772242, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24655647, Length=562, Percent_Identity=30.0711743772242, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24663005, Length=562, Percent_Identity=30.4270462633452, Blast_Score=201, Evalue=1e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 61093; Mature: 60962

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLDSTHHFDVVIVGSGGAGLSLALSLPSHYKIAILAKSSLTEASTFYAQGGVAAVLDK
CCCCCCCCCEEEEEEECCCCCEEEEEECCCCEEEEEEECCCCCHHHHHHHCCCEEEEECC
TDSIQQHIDDTMIAGAHLCELDAVKQTVEGGRPSVNFLLKHGVEFTLDEDDQLHLTREGG
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCEEEECCCCCEEEEECCC
HSQRRIIHAADATGKAISTTLVQRAKEASNLTIFENFIAIDIITLHKLGHIQQDNRAVGL
CCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEHHHHEEHHHHHHHCCCCCCCCEEEE
YVLDEQNEKVHTFLAPFIALACGGAMKAYLYTSNPDIATGDGIAMAYRAGCRVANMEFNQ
EEECCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEEEHHHCCCEEECCCCCC
FHPTCLYHPQARSFLITEAMRGEGAYLRLPDGERFMLRFDERAELAPRDIVARAIDYEIK
CCCEEEECCCCCHHHHHHHHCCCCCEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHH
RLGIRHVWLDITHKSAEFIQQHFPTLYTRLLELGIDITKDMIPVVPAAHYTCGGVVVNAQ
HCCEEEEEEEEECHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCEECCCEEEECC
SQTDIEGLYAIGETSYTGLHGANRMASNSLLECFVYGLSAAEDIQQKFEQDFNLPNIPEW
CCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCC
DDSQVTDADEDVVILQNWDELRSTMWNYVGIVRTTKRLQRALHRIEMLKREITEYYQDYR
CCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSKNLIELRNLVLVSEMIVRCAMQRKESRGLHYTLDYPELEQQLKKTVLTPPNFEVKQIP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHCCCCCCCEEEECC
VNTL
CCCC
>Mature Secondary Structure 
SNLDSTHHFDVVIVGSGGAGLSLALSLPSHYKIAILAKSSLTEASTFYAQGGVAAVLDK
CCCCCCCCEEEEEEECCCCCEEEEEECCCCEEEEEEECCCCCHHHHHHHCCCEEEEECC
TDSIQQHIDDTMIAGAHLCELDAVKQTVEGGRPSVNFLLKHGVEFTLDEDDQLHLTREGG
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCEEEECCCCCEEEEECCC
HSQRRIIHAADATGKAISTTLVQRAKEASNLTIFENFIAIDIITLHKLGHIQQDNRAVGL
CCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEHHHHEEHHHHHHHCCCCCCCCEEEE
YVLDEQNEKVHTFLAPFIALACGGAMKAYLYTSNPDIATGDGIAMAYRAGCRVANMEFNQ
EEECCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEEEHHHCCCEEECCCCCC
FHPTCLYHPQARSFLITEAMRGEGAYLRLPDGERFMLRFDERAELAPRDIVARAIDYEIK
CCCEEEECCCCCHHHHHHHHCCCCCEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHH
RLGIRHVWLDITHKSAEFIQQHFPTLYTRLLELGIDITKDMIPVVPAAHYTCGGVVVNAQ
HCCEEEEEEEEECHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCEECCCEEEECC
SQTDIEGLYAIGETSYTGLHGANRMASNSLLECFVYGLSAAEDIQQKFEQDFNLPNIPEW
CCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCC
DDSQVTDADEDVVILQNWDELRSTMWNYVGIVRTTKRLQRALHRIEMLKREITEYYQDYR
CCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSKNLIELRNLVLVSEMIVRCAMQRKESRGLHYTLDYPELEQQLKKTVLTPPNFEVKQIP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHCCCCCCCEEEECC
VNTL
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7737518; 10984043; 7961421 [H]