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The map label for this gene is pdxJ

Identifier: 50085652

GI number: 50085652

Start: 2536262

End: 2536987

Strand: Reverse

Name: pdxJ

Synonym: ACIAD2577

Alternate gene names: 50085652

Gene position: 2536987-2536262 (Counterclockwise)

Preceding gene: 50085653

Following gene: 50085651

Centisome position: 70.5

GC content: 46.97

Gene sequence:

>726_bases
ATGGCTGCATTGCTTGGTGTCAATATTGATCATGTAGCGACCTTGAGACAGGCGAGAGGAACACGTTATCCAGATCCAGT
GAGTGCTGCTTTGGTATGTGAGCAGGCAGGTGCTGAAGGAATTACTTTGCATCTACGTGAAGACCGTCGTCACATTCAGG
ATGATGATGTGCGTCGTATGCGTCCATTGCTTAAAACACGCATGAATCTAGAACTGGCAGTGACTGATGAGATGATTGCC
TTTGCCAAAGAAATTCAGCCACAGCACGTCTGCTTTGTACCTGAGCGTCGTCAGGAAGTCACCACAGAAGGTGGTCTGGA
TGTAGTCGGCCAGTTTGAAAAAGTCAAAGCGGCAACTCAAACGTTAGCGGCAGTGGGATGTGATGTTTCCCTATTTATTG
ATGCTGATCTTGCGCAAATTGATGCTGCGATTGCATGTGGTGCGCCAACCATTGAGTTACATACGGGTGCATATGCAGAT
GCTGAAACTGAAGAGCAGCAACAGGCAGAGTTGCAACGTATCATTGAAGGGGTTGAGTATGCAGCCAGCAAAGGATTGGT
GGTTAATGCAGGGCATGGTCTGAACCTTGATAATATCACGCCAATTGCAGCGATTCCGCAAATTCATGAGCTGAATATTG
GTCATTCAATTATTGCGGATAGTGTGTTTGTTGGATTGGCGCAAGCTGTACAGCATATGAAAGCTGCGATTCAGGCAGCA
CGATAA

Upstream 100 bases:

>100_bases
TACCGTCAAATGATTACATCTTTGTTGGGTGATCGACCTTTAAAGAGTCGCCAACTTTGGATTCAAAATACTCAATCTCA
TTCGTAAATAGGATTTTTTT

Downstream 100 bases:

>100_bases
GAGATTGAAGATGTCGAATCCGAACTATGATGAATTGATGCAACCTGTAATCGCATTTTTGGGTTGTGAAACACCTCAAG
CATGGTTAGATGAAGCGGTG

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase

Number of amino acids: Translated: 241; Mature: 240

Protein sequence:

>241_residues
MAALLGVNIDHVATLRQARGTRYPDPVSAALVCEQAGAEGITLHLREDRRHIQDDDVRRMRPLLKTRMNLELAVTDEMIA
FAKEIQPQHVCFVPERRQEVTTEGGLDVVGQFEKVKAATQTLAAVGCDVSLFIDADLAQIDAAIACGAPTIELHTGAYAD
AETEEQQQAELQRIIEGVEYAASKGLVVNAGHGLNLDNITPIAAIPQIHELNIGHSIIADSVFVGLAQAVQHMKAAIQAA
R

Sequences:

>Translated_241_residues
MAALLGVNIDHVATLRQARGTRYPDPVSAALVCEQAGAEGITLHLREDRRHIQDDDVRRMRPLLKTRMNLELAVTDEMIA
FAKEIQPQHVCFVPERRQEVTTEGGLDVVGQFEKVKAATQTLAAVGCDVSLFIDADLAQIDAAIACGAPTIELHTGAYAD
AETEEQQQAELQRIIEGVEYAASKGLVVNAGHGLNLDNITPIAAIPQIHELNIGHSIIADSVFVGLAQAVQHMKAAIQAA
R
>Mature_240_residues
AALLGVNIDHVATLRQARGTRYPDPVSAALVCEQAGAEGITLHLREDRRHIQDDDVRRMRPLLKTRMNLELAVTDEMIAF
AKEIQPQHVCFVPERRQEVTTEGGLDVVGQFEKVKAATQTLAAVGCDVSLFIDADLAQIDAAIACGAPTIELHTGAYADA
ETEEQQQAELQRIIEGVEYAASKGLVVNAGHGLNLDNITPIAAIPQIHELNIGHSIIADSVFVGLAQAVQHMKAAIQAAR

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family

Homologues:

Organism=Escherichia coli, GI1788917, Length=238, Percent_Identity=63.0252100840336, Blast_Score=297, Evalue=5e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXJ_ACIAD (Q3V7G6)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_047162.1
- ProteinModelPortal:   Q3V7G6
- SMR:   Q3V7G6
- STRING:   Q3V7G6
- GeneID:   2878300
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD2577
- NMPDR:   fig|62977.3.peg.2355
- eggNOG:   COG0854
- HOGENOM:   HBG299314
- OMA:   AGHGLNY
- PhylomeDB:   Q3V7G6
- ProtClustDB:   PRK05265
- BioCyc:   ASP62977:ACIAD2577-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00279
- InterPro:   IPR013785
- InterPro:   IPR004569
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00559

Pfam domain/function: PF03740 PdxJ; SSF63892 PyrdxlP_synth_PdxJ

EC number: =2.6.99.2

Molecular weight: Translated: 25994; Mature: 25863

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: ACT_SITE 44-44 ACT_SITE 71-71 ACT_SITE 192-192 BINDING 8-8 BINDING 19-19 BINDING 46-46 BINDING 51-51 BINDING 101-101 BINDING 193-193

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAALLGVNIDHVATLRQARGTRYPDPVSAALVCEQAGAEGITLHLREDRRHIQDDDVRRM
CCEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEEECHHCCCCCCHHHHH
RPLLKTRMNLELAVTDEMIAFAKEIQPQHVCFVPERRQEVTTEGGLDVVGQFEKVKAATQ
HHHHHHHCCCEEEEHHHHHHHHHHCCCCEEEECCCHHHHHCCCCCCHHHHHHHHHHHHHH
TLAAVGCDVSLFIDADLAQIDAAIACGAPTIELHTGAYADAETEEQQQAELQRIIEGVEY
HHHHHCCCEEEEEECCHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHH
AASKGLVVNAGHGLNLDNITPIAAIPQIHELNIGHSIIADSVFVGLAQAVQHMKAAIQAA
HHCCCEEEECCCCCCCCCCCCHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
R
C
>Mature Secondary Structure 
AALLGVNIDHVATLRQARGTRYPDPVSAALVCEQAGAEGITLHLREDRRHIQDDDVRRM
CEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEEECHHCCCCCCHHHHH
RPLLKTRMNLELAVTDEMIAFAKEIQPQHVCFVPERRQEVTTEGGLDVVGQFEKVKAATQ
HHHHHHHCCCEEEEHHHHHHHHHHCCCCEEEECCCHHHHHCCCCCCHHHHHHHHHHHHHH
TLAAVGCDVSLFIDADLAQIDAAIACGAPTIELHTGAYADAETEEQQQAELQRIIEGVEY
HHHHHCCCEEEEEECCHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHH
AASKGLVVNAGHGLNLDNITPIAAIPQIHELNIGHSIIADSVFVGLAQAVQHMKAAIQAA
HHCCCEEEECCCCCCCCCCCCHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
R
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA