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The map label for this gene is miaE [H]

Identifier: 50085651

GI number: 50085651

Start: 2535595

End: 2536284

Strand: Reverse

Name: miaE [H]

Synonym: ACIAD2576

Alternate gene names: 50085651

Gene position: 2536284-2535595 (Counterclockwise)

Preceding gene: 50085652

Following gene: 50085650

Centisome position: 70.48

GC content: 42.9

Gene sequence:

>690_bases
CTGCGATTCAGGCAGCACGATAAGAGATTGAAGATGTCGAATCCGAACTATGATGAATTGATGCAACCTGTAATCGCATT
TTTGGGTTGTGAAACACCTCAAGCATGGTTAGATGAAGCGGTGAATAATCTTGAGCTATTAATGCAGGATCATGCCAATT
GCGAAAAGAAGGCAGCAGGTACAGCCATGAACCTGATGTTCCGTTATAGTTTTTTTACCGACTTGCAGGTGAAGCTGGCT
CAGCTTGTACGCGAAGAAATGTTGCATTACGAGCAAGTGCTTGAGTTTATGAATAAGCGTGGACAAGCATGGAAAGCGGT
AAGTGCTGGTCGTTATGCAGGCGAGCTACGTAAAGAAATTCGAACCTATGAACCAGAAGCTTTGATTGATGTGTTGGTGG
TGGGTGCATTTGTAGAAGCACGCTCATGTGAACGTTTTCATGCATTAGCACCTTTGGTTGATGAAGAACTCGGACGTTAC
TATCGTTACTTGCTCAAGTCTGAATCACGGCATTTTGAAGACTATTTGGCCTTGGCACTTGAAGTTGCCAAAACCGCAAA
AATGAAAAATCCAGAAGAAGATATCGAGCAGCGTATTCAGCGTATTCGTGAAGTAGAAAATAATCTAATTTCAACGCCTG
ATGATGTTTTTCGTTTTCATAGTGGCGTGCCTCCATTTAAGGCGGCTTAA

Upstream 100 bases:

>100_bases
ATTGCAGCGATTCCGCAAATTCATGAGCTGAATATTGGTCATTCAATTATTGCGGATAGTGTGTTTGTTGGATTGGCGCA
AGCTGTACAGCATATGAAAG

Downstream 100 bases:

>100_bases
CTATTTAGTTTGATTAAAATCTCTGCATGTTTGCAGGGATTTTTTATGTGTTTAATTTAAATAAGCAAAAAAAAGCCCGC
TTTAATGCTGCGGGCTTCTT

Product: tRNA hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MRFRQHDKRLKMSNPNYDELMQPVIAFLGCETPQAWLDEAVNNLELLMQDHANCEKKAAGTAMNLMFRYSFFTDLQVKLA
QLVREEMLHYEQVLEFMNKRGQAWKAVSAGRYAGELRKEIRTYEPEALIDVLVVGAFVEARSCERFHALAPLVDEELGRY
YRYLLKSESRHFEDYLALALEVAKTAKMKNPEEDIEQRIQRIREVENNLISTPDDVFRFHSGVPPFKAA

Sequences:

>Translated_229_residues
MRFRQHDKRLKMSNPNYDELMQPVIAFLGCETPQAWLDEAVNNLELLMQDHANCEKKAAGTAMNLMFRYSFFTDLQVKLA
QLVREEMLHYEQVLEFMNKRGQAWKAVSAGRYAGELRKEIRTYEPEALIDVLVVGAFVEARSCERFHALAPLVDEELGRY
YRYLLKSESRHFEDYLALALEVAKTAKMKNPEEDIEQRIQRIREVENNLISTPDDVFRFHSGVPPFKAA
>Mature_229_residues
MRFRQHDKRLKMSNPNYDELMQPVIAFLGCETPQAWLDEAVNNLELLMQDHANCEKKAAGTAMNLMFRYSFFTDLQVKLA
QLVREEMLHYEQVLEFMNKRGQAWKAVSAGRYAGELRKEIRTYEPEALIDVLVVGAFVEARSCERFHALAPLVDEELGRY
YRYLLKSESRHFEDYLALALEVAKTAKMKNPEEDIEQRIQRIREVENNLISTPDDVFRFHSGVPPFKAA

Specific function: Production of the modified nucleoside 2-methylthio-cis- ribozeatin (MS[2]IO[6]A) found in some tRNAs. Catalyzes the oxygen-dependent transformation of MS[2]I[6]A into MS[2]IO[6]A [H]

COG id: COG4445

COG function: function code FJ; Hydroxylase for synthesis of 2-methylthio-cis-ribozeatin in tRNA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012347
- InterPro:   IPR010386 [H]

Pfam domain/function: PF06175 MiaE [H]

EC number: NA

Molecular weight: Translated: 26709; Mature: 26709

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFRQHDKRLKMSNPNYDELMQPVIAFLGCETPQAWLDEAVNNLELLMQDHANCEKKAAG
CCCCHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHH
TAMNLMFRYSFFTDLQVKLAQLVREEMLHYEQVLEFMNKRGQAWKAVSAGRYAGELRKEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
RTYEPEALIDVLVVGAFVEARSCERFHALAPLVDEELGRYYRYLLKSESRHFEDYLALAL
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVAKTAKMKNPEEDIEQRIQRIREVENNLISTPDDVFRFHSGVPPFKAA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRFRQHDKRLKMSNPNYDELMQPVIAFLGCETPQAWLDEAVNNLELLMQDHANCEKKAAG
CCCCHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHH
TAMNLMFRYSFFTDLQVKLAQLVREEMLHYEQVLEFMNKRGQAWKAVSAGRYAGELRKEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
RTYEPEALIDVLVVGAFVEARSCERFHALAPLVDEELGRYYRYLLKSESRHFEDYLALAL
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVAKTAKMKNPEEDIEQRIQRIREVENNLISTPDDVFRFHSGVPPFKAA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253666; 11677609 [H]