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The map label for this gene is amtB [H]

Identifier: 50085611

GI number: 50085611

Start: 2490601

End: 2491911

Strand: Direct

Name: amtB [H]

Synonym: ACIAD2534

Alternate gene names: 50085611

Gene position: 2490601-2491911 (Clockwise)

Preceding gene: 50085610

Following gene: 50085624

Centisome position: 69.21

GC content: 46.61

Gene sequence:

>1311_bases
ATGTCCTCCGATATACTCAAAAAATTAGGATTGTGTCTGTTAACGCTTATGCCTGCTTATGCATGTGCTGCCGATACGCC
TTTTGATGCTGCTTCGACTGTATGGCTCATGGTTTCTGCAATTTTGGTGCTGATCATGTTTATTCCTGGTCTGGCACTGT
TTTATGGCGGAATGCTCAGAGCAAAAAATGTGCTGTCTATTTTTTCCCAGTTTTTTGCCGTTGCAGGTGCCGTCGGTATT
TTATGGATCGCAATTGTGTATAGCATTGTGGCAGATACTACGCATATGAGTGAAGGCAGCCTTAATCTGAATAGCTTTAT
AGGAGGCTTTGGCAAAGCATTTATGGCAGGAATTACAGACCAGACTCTCATTGCTGGAGTACCAGAATACGTGGTGTGTA
TTTTTGGTTTGACCTTCGCCATGATTACACCCTGTATTGCCCTTGGTGGTTTTGCAGAACGAATGAAGTTTGGTGCTGTG
GTGTTGTTTGGTGCACTCTGGCTGGTTTTGGTCTATGGGCCTTTGGCACATATGGTCTGGGGCGGTCCAGGCGCGATTAT
GCATAACTGGGGCGTACTTGATTTTGCAGGTGGAACGGCTGTACACATCAATTCGGGTGTTGCTGCTCTGGTAGGTGCAA
TCGTTGTTGGGCAACGTCGTGGTTGGCCGACCACAGCCATGCCTCCTCACAATCTGGTGTTTACCATGATGGGCGCTGCT
CTATTATGGGCAGGCTGGTACGGCTTCAATGTCGGCTCAGCGCTGGCAGCAAATACCACAGCGGGACTGGTACTCATGAC
CACCACCATTGCAACTTGCGGTGGAATTGTAGGCTGGATAATGATTGAAAAAATCATGACTCAACAAGTGACCTCATTGG
GGCTTGCGTCTGGTGTCATTGCAGGTTTGGTTGGTATTACCCCTGCAGCTGCTTATGTTGGCCCTTTTGGTGCAATCGCA
GTCGGTTTGATAACCAGCTTATGTTGCTTCTTTGCCGTAACAGGGCTCAAACGCAAATTTGGTTTCGATGATGCTTTGGA
TGTATTTGCGCTACATGGTATTGGGGGAATGGCGGGCTGTATTCTGACCGGAATCTTTTGCATTCCTGCACTGGGCGGCA
ATCAGGATGTTGATCTGGTTAAACAGCTCACAGCTCAAATTGCCAGTATTGCTCTAACCGTCATTTACTGTGGTGTATTC
ACTTGGATTATTATGAAGATTATCGACAAAACTATTGGTTTACGCGTTACATCTGAACAGGAACAACAAGGACTCGATGT
CAGTGATCATAATGAACGTGCCTACAATTAA

Upstream 100 bases:

>100_bases
ACCATCATGGTATAAGAATTGCTTTAATAAAATGAAATTAATATTCCCATTAGGAATAAAAAGATTTTTTCATTTTCTTT
TCCCGTTAGGAGTCTCCATC

Downstream 100 bases:

>100_bases
ATACGCTATGTGATAATTGACGGTTATATTTATCCAGATCCATCAAAAATAAGAACCTAAATTTAGGTTCTTATTTTTTG
CAGGTTTTAATGATTAATCT

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MSSDILKKLGLCLLTLMPAYACAADTPFDAASTVWLMVSAILVLIMFIPGLALFYGGMLRAKNVLSIFSQFFAVAGAVGI
LWIAIVYSIVADTTHMSEGSLNLNSFIGGFGKAFMAGITDQTLIAGVPEYVVCIFGLTFAMITPCIALGGFAERMKFGAV
VLFGALWLVLVYGPLAHMVWGGPGAIMHNWGVLDFAGGTAVHINSGVAALVGAIVVGQRRGWPTTAMPPHNLVFTMMGAA
LLWAGWYGFNVGSALAANTTAGLVLMTTTIATCGGIVGWIMIEKIMTQQVTSLGLASGVIAGLVGITPAAAYVGPFGAIA
VGLITSLCCFFAVTGLKRKFGFDDALDVFALHGIGGMAGCILTGIFCIPALGGNQDVDLVKQLTAQIASIALTVIYCGVF
TWIIMKIIDKTIGLRVTSEQEQQGLDVSDHNERAYN

Sequences:

>Translated_436_residues
MSSDILKKLGLCLLTLMPAYACAADTPFDAASTVWLMVSAILVLIMFIPGLALFYGGMLRAKNVLSIFSQFFAVAGAVGI
LWIAIVYSIVADTTHMSEGSLNLNSFIGGFGKAFMAGITDQTLIAGVPEYVVCIFGLTFAMITPCIALGGFAERMKFGAV
VLFGALWLVLVYGPLAHMVWGGPGAIMHNWGVLDFAGGTAVHINSGVAALVGAIVVGQRRGWPTTAMPPHNLVFTMMGAA
LLWAGWYGFNVGSALAANTTAGLVLMTTTIATCGGIVGWIMIEKIMTQQVTSLGLASGVIAGLVGITPAAAYVGPFGAIA
VGLITSLCCFFAVTGLKRKFGFDDALDVFALHGIGGMAGCILTGIFCIPALGGNQDVDLVKQLTAQIASIALTVIYCGVF
TWIIMKIIDKTIGLRVTSEQEQQGLDVSDHNERAYN
>Mature_435_residues
SSDILKKLGLCLLTLMPAYACAADTPFDAASTVWLMVSAILVLIMFIPGLALFYGGMLRAKNVLSIFSQFFAVAGAVGIL
WIAIVYSIVADTTHMSEGSLNLNSFIGGFGKAFMAGITDQTLIAGVPEYVVCIFGLTFAMITPCIALGGFAERMKFGAVV
LFGALWLVLVYGPLAHMVWGGPGAIMHNWGVLDFAGGTAVHINSGVAALVGAIVVGQRRGWPTTAMPPHNLVFTMMGAAL
LWAGWYGFNVGSALAANTTAGLVLMTTTIATCGGIVGWIMIEKIMTQQVTSLGLASGVIAGLVGITPAAAYVGPFGAIAV
GLITSLCCFFAVTGLKRKFGFDDALDVFALHGIGGMAGCILTGIFCIPALGGNQDVDLVKQLTAQIASIALTVIYCGVFT
WIIMKIIDKTIGLRVTSEQEQQGLDVSDHNERAYN

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=432, Percent_Identity=43.5185185185185, Blast_Score=331, Evalue=5e-92,
Organism=Caenorhabditis elegans, GI32564042, Length=426, Percent_Identity=27.4647887323944, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI133931100, Length=460, Percent_Identity=25.8695652173913, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17550182, Length=447, Percent_Identity=24.1610738255034, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17550180, Length=444, Percent_Identity=24.7747747747748, Blast_Score=82, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6324187, Length=435, Percent_Identity=33.3333333333333, Blast_Score=191, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6325396, Length=422, Percent_Identity=32.7014218009479, Blast_Score=189, Evalue=8e-49,
Organism=Saccharomyces cerevisiae, GI6321559, Length=424, Percent_Identity=29.4811320754717, Blast_Score=169, Evalue=5e-43,
Organism=Drosophila melanogaster, GI161078314, Length=419, Percent_Identity=27.2076372315036, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 45633; Mature: 45502

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SSDILKKLGLCLLTLMPAYACAADTPFDAASTVWLMVSAILVLIMFIPGLALFYGGMLR
CHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKNVLSIFSQFFAVAGAVGILWIAIVYSIVADTTHMSEGSLNLNSFIGGFGKAFMAGITD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCC
QTLIAGVPEYVVCIFGLTFAMITPCIALGGFAERMKFGAVVLFGALWLVLVYGPLAHMVW
HHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GGPGAIMHNWGVLDFAGGTAVHINSGVAALVGAIVVGQRRGWPTTAMPPHNLVFTMMGAA
CCCCHHHCCCCCEEECCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
LLWAGWYGFNVGSALAANTTAGLVLMTTTIATCGGIVGWIMIEKIMTQQVTSLGLASGVI
HHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGLVGITPAAAYVGPFGAIAVGLITSLCCFFAVTGLKRKFGFDDALDVFALHGIGGMAGC
HHHHHHCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHH
ILTGIFCIPALGGNQDVDLVKQLTAQIASIALTVIYCGVFTWIIMKIIDKTIGLRVTSEQ
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCH
EQQGLDVSDHNERAYN
HHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]