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The map label for this gene is dnaB [H]

Identifier: 50085518

GI number: 50085518

Start: 2397789

End: 2399234

Strand: Direct

Name: dnaB [H]

Synonym: ACIAD2433

Alternate gene names: 50085518

Gene position: 2397789-2399234 (Clockwise)

Preceding gene: 50085517

Following gene: 50085519

Centisome position: 66.63

GC content: 39.76

Gene sequence:

>1446_bases
ATGTCGCAGGCGAATGCCAATTTTTTGGATAACTCAAAAGTTTCAGCCAATAAAACTGCTGAATCAGGTCAGTTAAAAGA
ATTTCGTACTCCGCCACATAACTTAGCAATAGAGCAGGCTGTACTTGCAGCTTTGATGACTGTTGCTGAATCATTCGAGC
AAGTGAACGATTTGCTCAAAGAAAATGATTTTTATGCGACACGCCACAAATATATTTTTCGTGCTATTGAAAAATTAGCT
CAAGAAAACTCACCTTATGATGCAGTACTGGTAAATGATTGGTTACTTAAACAAAATTTGCTAGACGCAATTGGTGGTGA
AGAGTATTTGATGCAACTCATGGCAGACTCTCCATCAAGTTTTTATAATCTTGAGACATATGCAAGCAAGATTAAAGAAT
TTTCAACCTTGCGTAGCATGATTAAGGTCAGTAGCGATATTTTGCAAAATGCTTATGACACCAAAGGGCGTCCAGTCAGT
GAAATTTTAGATTTAGCCGAAACCAGTATTTTTTCACTTGCCGAGCAGCATAATAACAATAAGAAAGATGCAGGGCCTAA
ATCGATTAGCTCAGTAACAGCAGATGTAATTAGCAAATTAGATGAATTGTCTAAACTTGACGGTAATATTACAGGTCTAA
CCACAGGCTTTTTAGAGCTCGACAATAAAACCTATGGTATGCAATCTGGTGACATGATTATTGTTGCAGCACGTCCATCC
ATGGGTAAAACGACTTTTGCCATGAATCTGGTCGAGAGCGTGCTATTTAACTGTAATCTACCTGCACTGGTATTCTCGAT
GGAAATGCCAGCAGATTCAATTGCCATGCGTTTGATTTCATCTTACGGTAAGGTTCATCAAGGTCACCTACGCTCTGGTA
AGCTGGATGGTGATGAATGGTCAAAGGTAACAGGTACGATTCTCCAGCTTCAAGAGAAGCATTTATATATTGATGACTCC
TCTGCTTTACCCCCTACCGAACTCCGTGCTCGTGCAAGACGTATTGCAAAGCAACATGGTGGAAAATTGGGTTGTATCAT
GATCGATTATCTACAATTGATGAAAGTTCCTGGTATGGGCGATAACCGTGTAGGTGAAATTGGCGAAATCTCACGAAGCT
TAAAAGCATTAGCCAAAGAAATGGACTGTCCTGTCATTGCGCTTTCTCAGCTTAACCGAAGTCTTGAAAATCGCCCCAAT
AAACGTCCAGTAATGTCCGATTTACGTGAATCTGGTGCAATCGAACAGGATGCTGACTTGATCATGTTCATTTATCGTGA
TGAGGTCTATAACAAAGAATCTAAAGAAGCAGGTACAGCCGAAATTATTATTGGTAAACAGCGTAATGGTCCTATTGGCA
CAGTTCGTCTAGCTTTTGAAGGTCAATATACTCGGTTTAGTAACCTCTCACCTGAATATTACAGTCAGTATGATGACGAA
GAATAA

Upstream 100 bases:

>100_bases
ATAAGTTGTTTTTTCAATAAAACCCCCTTCTCTATCTTTTTTGCATTAAGATAGTTCGGCTATTTCAAAACTCTTTCCAA
TTTTAAGATCAGGTTACTTT

Downstream 100 bases:

>100_bases
ATTACATTACTATACACAGGAGTAATCAGGGTGCGCCATGCAACAGTTTATATTGATCGCCAAGCGCTTCGATATAATTT
AAACCGTGTCAAACAACTTG

Product: replicative DNA helicase;chromosome replication, chain elongation

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MSQANANFLDNSKVSANKTAESGQLKEFRTPPHNLAIEQAVLAALMTVAESFEQVNDLLKENDFYATRHKYIFRAIEKLA
QENSPYDAVLVNDWLLKQNLLDAIGGEEYLMQLMADSPSSFYNLETYASKIKEFSTLRSMIKVSSDILQNAYDTKGRPVS
EILDLAETSIFSLAEQHNNNKKDAGPKSISSVTADVISKLDELSKLDGNITGLTTGFLELDNKTYGMQSGDMIIVAARPS
MGKTTFAMNLVESVLFNCNLPALVFSMEMPADSIAMRLISSYGKVHQGHLRSGKLDGDEWSKVTGTILQLQEKHLYIDDS
SALPPTELRARARRIAKQHGGKLGCIMIDYLQLMKVPGMGDNRVGEIGEISRSLKALAKEMDCPVIALSQLNRSLENRPN
KRPVMSDLRESGAIEQDADLIMFIYRDEVYNKESKEAGTAEIIIGKQRNGPIGTVRLAFEGQYTRFSNLSPEYYSQYDDE
E

Sequences:

>Translated_481_residues
MSQANANFLDNSKVSANKTAESGQLKEFRTPPHNLAIEQAVLAALMTVAESFEQVNDLLKENDFYATRHKYIFRAIEKLA
QENSPYDAVLVNDWLLKQNLLDAIGGEEYLMQLMADSPSSFYNLETYASKIKEFSTLRSMIKVSSDILQNAYDTKGRPVS
EILDLAETSIFSLAEQHNNNKKDAGPKSISSVTADVISKLDELSKLDGNITGLTTGFLELDNKTYGMQSGDMIIVAARPS
MGKTTFAMNLVESVLFNCNLPALVFSMEMPADSIAMRLISSYGKVHQGHLRSGKLDGDEWSKVTGTILQLQEKHLYIDDS
SALPPTELRARARRIAKQHGGKLGCIMIDYLQLMKVPGMGDNRVGEIGEISRSLKALAKEMDCPVIALSQLNRSLENRPN
KRPVMSDLRESGAIEQDADLIMFIYRDEVYNKESKEAGTAEIIIGKQRNGPIGTVRLAFEGQYTRFSNLSPEYYSQYDDE
E
>Mature_480_residues
SQANANFLDNSKVSANKTAESGQLKEFRTPPHNLAIEQAVLAALMTVAESFEQVNDLLKENDFYATRHKYIFRAIEKLAQ
ENSPYDAVLVNDWLLKQNLLDAIGGEEYLMQLMADSPSSFYNLETYASKIKEFSTLRSMIKVSSDILQNAYDTKGRPVSE
ILDLAETSIFSLAEQHNNNKKDAGPKSISSVTADVISKLDELSKLDGNITGLTTGFLELDNKTYGMQSGDMIIVAARPSM
GKTTFAMNLVESVLFNCNLPALVFSMEMPADSIAMRLISSYGKVHQGHLRSGKLDGDEWSKVTGTILQLQEKHLYIDDSS
ALPPTELRARARRIAKQHGGKLGCIMIDYLQLMKVPGMGDNRVGEIGEISRSLKALAKEMDCPVIALSQLNRSLENRPNK
RPVMSDLRESGAIEQDADLIMFIYRDEVYNKESKEAGTAEIIIGKQRNGPIGTVRLAFEGQYTRFSNLSPEYYSQYDDEE

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=460, Percent_Identity=51.304347826087, Blast_Score=475, Evalue=1e-135,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 53562; Mature: 53431

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQANANFLDNSKVSANKTAESGQLKEFRTPPHNLAIEQAVLAALMTVAESFEQVNDLLK
CCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ENDFYATRHKYIFRAIEKLAQENSPYDAVLVNDWLLKQNLLDAIGGEEYLMQLMADSPSS
CCCCHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHCCHHHHHHHHHCCCCH
FYNLETYASKIKEFSTLRSMIKVSSDILQNAYDTKGRPVSEILDLAETSIFSLAEQHNNN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCC
KKDAGPKSISSVTADVISKLDELSKLDGNITGLTTGFLELDNKTYGMQSGDMIIVAARPS
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCEEEEEECCC
MGKTTFAMNLVESVLFNCNLPALVFSMEMPADSIAMRLISSYGKVHQGHLRSGKLDGDEW
CCCHHHHHHHHHHHHHCCCCCHHEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
SKVTGTILQLQEKHLYIDDSSALPPTELRARARRIAKQHGGKLGCIMIDYLQLMKVPGMG
HHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCC
DNRVGEIGEISRSLKALAKEMDCPVIALSQLNRSLENRPNKRPVMSDLRESGAIEQDADL
CCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCE
IMFIYRDEVYNKESKEAGTAEIIIGKQRNGPIGTVRLAFEGQYTRFSNLSPEYYSQYDDE
EEEEEHHHHHCCCCCCCCCEEEEEECCCCCCCEEEEEEECCCCCCCCCCCHHHHHCCCCC
E
C
>Mature Secondary Structure 
SQANANFLDNSKVSANKTAESGQLKEFRTPPHNLAIEQAVLAALMTVAESFEQVNDLLK
CCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ENDFYATRHKYIFRAIEKLAQENSPYDAVLVNDWLLKQNLLDAIGGEEYLMQLMADSPSS
CCCCHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHCCHHHHHHHHHCCCCH
FYNLETYASKIKEFSTLRSMIKVSSDILQNAYDTKGRPVSEILDLAETSIFSLAEQHNNN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCC
KKDAGPKSISSVTADVISKLDELSKLDGNITGLTTGFLELDNKTYGMQSGDMIIVAARPS
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCEEEEEECCC
MGKTTFAMNLVESVLFNCNLPALVFSMEMPADSIAMRLISSYGKVHQGHLRSGKLDGDEW
CCCHHHHHHHHHHHHHCCCCCHHEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
SKVTGTILQLQEKHLYIDDSSALPPTELRARARRIAKQHGGKLGCIMIDYLQLMKVPGMG
HHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCC
DNRVGEIGEISRSLKALAKEMDCPVIALSQLNRSLENRPNKRPVMSDLRESGAIEQDADL
CCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCE
IMFIYRDEVYNKESKEAGTAEIIIGKQRNGPIGTVRLAFEGQYTRFSNLSPEYYSQYDDE
EEEEEHHHHHCCCCCCCCCEEEEEECCCCCCCEEEEEEECCCCCCCCCCCHHHHHCCCCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]