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The map label for this gene is moeB [H]

Identifier: 50085497

GI number: 50085497

Start: 2370755

End: 2371567

Strand: Reverse

Name: moeB [H]

Synonym: ACIAD2411

Alternate gene names: 50085497

Gene position: 2371567-2370755 (Counterclockwise)

Preceding gene: 50085499

Following gene: 50085496

Centisome position: 65.9

GC content: 39.98

Gene sequence:

>813_bases
GTGAACGATCAGGACAATCTGGATTTAGAGCTTAACGATGCAGAAATGCATTTATATAGCCGTCAAATTTTACTAGATGG
ATGGGATGTTGATGCTCAGGAAAAATTAAAATTTGCCAATGTCTTGATTGTTGGAGCGGGTGGAATTGGCTGTACGTCGG
CTGAGTTGCTTGCACGTGCTGGGGTCGGCAAAATTACCCTCATTGACTCAGATACGATTGAAATCAGTAATCTTCAGCGG
CAGATCGCATTTACACCTAATGATTTAGGATGTTTCAAAGCCGAAGTGCTTGCAAAACGTTTAACCCAGCTCAACCCGCA
TATCGTTGTTGAATATATTGTAAACAAATTAGTATCTGAAAATGTGGATGAGTTGATTCAGCATCAAGATTTAATCCTTG
ATGGTTGTGATAACTTTACAACACGCTATTTGGTCAATGCTTCTTGTAAGAAAAATCAAATACCTTTGATTAGTGCATCT
GCAATTGGATTTCAGGGTCAGTTATTCATGATAGATTCTGATTCTGCATGCTATGAGTGCTTGTTTCCAAAAGATCAGTT
TGAAAATGAGGGAATGCGCTGTGCCGAATCTGGGGTTCTAGCCACCACACCCGTAGTCATGGCATCGTTGCAGGCACATC
ATGCGCTACTCTATCTTGGTCTTGATCGTTCGCCATTAAGAGAAAAACTTTTACTGTGGGATGGTATGAGCATGAAACAA
CGAATTTTACATTTTGAAAAAGATACAAATTGTTCAACTTGTCAGGCAAGTTTATCGGTAAACTCGGTGAAGTTTGATAC
ACTGCCACAATGA

Upstream 100 bases:

>100_bases
GTAAAAGCTTACTTTTGTCAAAATAAAACATCTCACGACACAAGCTTCTCTTTATAATTATAGTTATAAATTTAGTATTC
ATAAATTGGGATAATCGCGA

Downstream 100 bases:

>100_bases
GTTAAACCATATTGAATCATATGAAAAAAAATATACTGTTTGATGGGCTACGATCTGTTGCACGTATTGGTGAAACTGCT
TTTGTCGCGGCAAGAGCAGG

Product: molybdopterin biosynthesis protein (moeB) or thiamin-thiazole moiety synthesis (thiF)

Products: NA

Alternate protein names: MoaD protein adenylase; Molybdopterin-converting factor subunit 1 adenylase [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MNDQDNLDLELNDAEMHLYSRQILLDGWDVDAQEKLKFANVLIVGAGGIGCTSAELLARAGVGKITLIDSDTIEISNLQR
QIAFTPNDLGCFKAEVLAKRLTQLNPHIVVEYIVNKLVSENVDELIQHQDLILDGCDNFTTRYLVNASCKKNQIPLISAS
AIGFQGQLFMIDSDSACYECLFPKDQFENEGMRCAESGVLATTPVVMASLQAHHALLYLGLDRSPLREKLLLWDGMSMKQ
RILHFEKDTNCSTCQASLSVNSVKFDTLPQ

Sequences:

>Translated_270_residues
MNDQDNLDLELNDAEMHLYSRQILLDGWDVDAQEKLKFANVLIVGAGGIGCTSAELLARAGVGKITLIDSDTIEISNLQR
QIAFTPNDLGCFKAEVLAKRLTQLNPHIVVEYIVNKLVSENVDELIQHQDLILDGCDNFTTRYLVNASCKKNQIPLISAS
AIGFQGQLFMIDSDSACYECLFPKDQFENEGMRCAESGVLATTPVVMASLQAHHALLYLGLDRSPLREKLLLWDGMSMKQ
RILHFEKDTNCSTCQASLSVNSVKFDTLPQ
>Mature_270_residues
MNDQDNLDLELNDAEMHLYSRQILLDGWDVDAQEKLKFANVLIVGAGGIGCTSAELLARAGVGKITLIDSDTIEISNLQR
QIAFTPNDLGCFKAEVLAKRLTQLNPHIVVEYIVNKLVSENVDELIQHQDLILDGCDNFTTRYLVNASCKKNQIPLISAS
AIGFQGQLFMIDSDSACYECLFPKDQFENEGMRCAESGVLATTPVVMASLQAHHALLYLGLDRSPLREKLLLWDGMSMKQ
RILHFEKDTNCSTCQASLSVNSVKFDTLPQ

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein moaD [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=228, Percent_Identity=38.5964912280702, Blast_Score=128, Evalue=6e-30,
Organism=Homo sapiens, GI4885649, Length=153, Percent_Identity=35.9477124183007, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI23510338, Length=162, Percent_Identity=32.0987654320988, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI23510340, Length=162, Percent_Identity=32.0987654320988, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI38045942, Length=172, Percent_Identity=34.3023255813954, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI38045944, Length=172, Percent_Identity=34.3023255813954, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI150417996, Length=148, Percent_Identity=33.1081081081081, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI38045948, Length=157, Percent_Identity=35.6687898089172, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI13376212, Length=226, Percent_Identity=30.9734513274336, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI38327032, Length=214, Percent_Identity=30.8411214953271, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1787048, Length=247, Percent_Identity=39.2712550607287, Blast_Score=177, Evalue=9e-46,
Organism=Escherichia coli, GI87082356, Length=249, Percent_Identity=40.5622489959839, Blast_Score=169, Evalue=1e-43,
Organism=Escherichia coli, GI1789177, Length=128, Percent_Identity=35.9375, Blast_Score=92, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17540406, Length=239, Percent_Identity=39.7489539748954, Blast_Score=167, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI193203301, Length=153, Percent_Identity=33.9869281045752, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI86565433, Length=157, Percent_Identity=31.8471337579618, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI86565431, Length=157, Percent_Identity=31.8471337579618, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71984476, Length=173, Percent_Identity=31.7919075144509, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17508819, Length=187, Percent_Identity=31.0160427807487, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6321903, Length=241, Percent_Identity=35.2697095435685, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6322639, Length=164, Percent_Identity=31.0975609756098, Blast_Score=76, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6320598, Length=152, Percent_Identity=31.5789473684211, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6325438, Length=161, Percent_Identity=27.9503105590062, Blast_Score=69, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24582879, Length=260, Percent_Identity=35.7692307692308, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24660640, Length=156, Percent_Identity=36.5384615384615, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24641311, Length=240, Percent_Identity=31.25, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI20129973, Length=180, Percent_Identity=33.3333333333333, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI28573937, Length=164, Percent_Identity=31.7073170731707, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR012730
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: NA

Molecular weight: Translated: 29970; Mature: 29970

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDQDNLDLELNDAEMHLYSRQILLDGWDVDAQEKLKFANVLIVGAGGIGCTSAELLARA
CCCCCCCEEEECCHHEEEEEEEEEEECCCCCHHHHHEEEEEEEEECCCCCCCHHHHHHHC
GVGKITLIDSDTIEISNLQRQIAFTPNDLGCFKAEVLAKRLTQLNPHIVVEYIVNKLVSE
CCCEEEEEECCCEEECCHHEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
NVDELIQHQDLILDGCDNFTTRYLVNASCKKNQIPLISASAIGFQGQLFMIDSDSACYEC
CHHHHHHHHHHEEECCCCCCEEEEEECCCCCCCCCEEEECCCCCCCEEEEEECCCCEEEE
LFPKDQFENEGMRCAESGVLATTPVVMASLQAHHALLYLGLDRSPLREKLLLWDGMSMKQ
CCCCHHHCCCCCEEHHCCCEEECHHHHHHHHHCEEEEEEECCCCHHHHHHHHCCCCCHHH
RILHFEKDTNCSTCQASLSVNSVKFDTLPQ
HHHHHCCCCCCCCEEEECEECEEEECCCCC
>Mature Secondary Structure
MNDQDNLDLELNDAEMHLYSRQILLDGWDVDAQEKLKFANVLIVGAGGIGCTSAELLARA
CCCCCCCEEEECCHHEEEEEEEEEEECCCCCHHHHHEEEEEEEEECCCCCCCHHHHHHHC
GVGKITLIDSDTIEISNLQRQIAFTPNDLGCFKAEVLAKRLTQLNPHIVVEYIVNKLVSE
CCCEEEEEECCCEEECCHHEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
NVDELIQHQDLILDGCDNFTTRYLVNASCKKNQIPLISASAIGFQGQLFMIDSDSACYEC
CHHHHHHHHHHEEECCCCCCEEEEEECCCCCCCCCEEEECCCCCCCEEEEEECCCCEEEE
LFPKDQFENEGMRCAESGVLATTPVVMASLQAHHALLYLGLDRSPLREKLLLWDGMSMKQ
CCCCHHHCCCCCEEHHCCCEEECHHHHHHHHHCEEEEEEECCCCHHHHHHHHCCCCCHHH
RILHFEKDTNCSTCQASLSVNSVKFDTLPQ
HHHHHCCCCCCCCEEEECEECEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3045084; 8905232; 9278503 [H]