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The map label for this gene is miaA

Identifier: 50085464

GI number: 50085464

Start: 2333168

End: 2334112

Strand: Reverse

Name: miaA

Synonym: ACIAD2374

Alternate gene names: 50085464

Gene position: 2334112-2333168 (Counterclockwise)

Preceding gene: 50085465

Following gene: 50085463

Centisome position: 64.86

GC content: 39.47

Gene sequence:

>945_bases
ATGTCATCGACTTTACCCGTCATTAATTTAATGGGGCCTACTGCAAGCGGGAAAACCGCTTTGGCGTGTGAACTCTATGA
ACGGGGTCATTTTGAGCTAATTTCAGTTGATTCTGCATTGATTTATAAAGATATGGATATCGGAACAGCTAAACCGACCA
AAGAAGAGCTGGCACTCTATCCACATCATCTCATTGATATTATTACGCCGTTAGAAGTGTATTCTGCCGCCCAATTTGTT
GAAGATGCAACGCAATTGATTGACCAAATTCACATGAAAGGTAAGACGCCTATTCTTGTGGGAGGGACGATGCTTTACTT
TAAGTCCTTACTTGAAGGGCTTTCAACTAATCTACCCAGTGCAGATGCAGAAGTACGCGCTGCAATTGAAAAACAGGCAG
AACAGGAAGGCTGGCAAAGTGTATATGATGAGCTTGCTCGTGTAGATCCTGTTGCGGGAGCCAGATTTAAAGTTTCTGAT
CGACAGCGGATTATTCGTGCAATTGAAGTTTATCGTTTGACAGGGCAGCCAATTACCAAATTGCAGGCAGAACAGCCTAA
AAACGTACCATATCGTTACACATTTCATAATTATGCATTGCTACCAGATCGGATAGAATTACATAAACGGATTGAGCAAA
GATTGTTCAAAATGTGGAGTATCGGTTTTTTAAATGAAGTTGAATCATTGATTAAAAAATACGATTTGAATGATAATTTA
CCGTCTATGCGATCAGTAGGTTATCGCCAAGCACTCGATTTTCTACTAAAAAGCGATATGAGTCACAATAATAGGAGGGA
AATGGAAGATAAAGCATTATTTGCAACACGACAACTTGCAAAACGTCAATACACTTGGTTACGTTCTTTGCAAGAAACGC
ACTATTTTAAAACCTATTTAACGATTAAGCAGGCTCAAGAAGACTTGCGAAACTCTTATGGATAA

Upstream 100 bases:

>100_bases
AACTGAATTTGCTAGCCAATGTAACCATGGTCGTCCTACATGGCGCGCATTTCCACTTCATCAACTTGATAAGCTTTTTG
CTCGAGGAGAATAATTCAAA

Downstream 100 bases:

>100_bases
AGCAAAATTTACATACTATCTTTTTTATAAATTTTAATTGAAAAATAAAGATGGTTTTTGCGCTGATTTTAAATTTTTTG
GAGTTATAAAATGTCTAAAG

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MSSTLPVINLMGPTASGKTALACELYERGHFELISVDSALIYKDMDIGTAKPTKEELALYPHHLIDIITPLEVYSAAQFV
EDATQLIDQIHMKGKTPILVGGTMLYFKSLLEGLSTNLPSADAEVRAAIEKQAEQEGWQSVYDELARVDPVAGARFKVSD
RQRIIRAIEVYRLTGQPITKLQAEQPKNVPYRYTFHNYALLPDRIELHKRIEQRLFKMWSIGFLNEVESLIKKYDLNDNL
PSMRSVGYRQALDFLLKSDMSHNNRREMEDKALFATRQLAKRQYTWLRSLQETHYFKTYLTIKQAQEDLRNSYG

Sequences:

>Translated_314_residues
MSSTLPVINLMGPTASGKTALACELYERGHFELISVDSALIYKDMDIGTAKPTKEELALYPHHLIDIITPLEVYSAAQFV
EDATQLIDQIHMKGKTPILVGGTMLYFKSLLEGLSTNLPSADAEVRAAIEKQAEQEGWQSVYDELARVDPVAGARFKVSD
RQRIIRAIEVYRLTGQPITKLQAEQPKNVPYRYTFHNYALLPDRIELHKRIEQRLFKMWSIGFLNEVESLIKKYDLNDNL
PSMRSVGYRQALDFLLKSDMSHNNRREMEDKALFATRQLAKRQYTWLRSLQETHYFKTYLTIKQAQEDLRNSYG
>Mature_313_residues
SSTLPVINLMGPTASGKTALACELYERGHFELISVDSALIYKDMDIGTAKPTKEELALYPHHLIDIITPLEVYSAAQFVE
DATQLIDQIHMKGKTPILVGGTMLYFKSLLEGLSTNLPSADAEVRAAIEKQAEQEGWQSVYDELARVDPVAGARFKVSDR
QRIIRAIEVYRLTGQPITKLQAEQPKNVPYRYTFHNYALLPDRIELHKRIEQRLFKMWSIGFLNEVESLIKKYDLNDNLP
SMRSVGYRQALDFLLKSDMSHNNRREMEDKALFATRQLAKRQYTWLRSLQETHYFKTYLTIKQAQEDLRNSYG

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=315, Percent_Identity=26.6666666666667, Blast_Score=116, Evalue=2e-26,
Organism=Escherichia coli, GI1790613, Length=298, Percent_Identity=52.0134228187919, Blast_Score=280, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI25144712, Length=243, Percent_Identity=29.2181069958848, Blast_Score=98, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6324848, Length=293, Percent_Identity=27.6450511945392, Blast_Score=112, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24649830, Length=285, Percent_Identity=31.5789473684211, Blast_Score=130, Evalue=9e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_ACIAD (Q6F9W1)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_046974.1
- ProteinModelPortal:   Q6F9W1
- SMR:   Q6F9W1
- STRING:   Q6F9W1
- GeneID:   2879538
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD2374
- NMPDR:   fig|62977.3.peg.2261
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- PhylomeDB:   Q6F9W1
- ProtClustDB:   PRK00091
- BioCyc:   ASP62977:ACIAD2374-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 36124; Mature: 35993

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTLPVINLMGPTASGKTALACELYERGHFELISVDSALIYKDMDIGTAKPTKEELALY
CCCCCCEEEECCCCCCCCCEEEHHHHHCCCEEEEEECCCCEEECCCCCCCCCCHHHHHHH
PHHLIDIITPLEVYSAAQFVEDATQLIDQIHMKGKTPILVGGTMLYFKSLLEGLSTNLPS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHCCCCC
ADAEVRAAIEKQAEQEGWQSVYDELARVDPVAGARFKVSDRQRIIRAIEVYRLTGQPITK
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCHHH
LQAEQPKNVPYRYTFHNYALLPDRIELHKRIEQRLFKMWSIGFLNEVESLIKKYDLNDNL
HCCCCCCCCCEEEEECCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PSMRSVGYRQALDFLLKSDMSHNNRREMEDKALFATRQLAKRQYTWLRSLQETHYFKTYL
CHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TIKQAQEDLRNSYG
HHHHHHHHHHHCCC
>Mature Secondary Structure 
SSTLPVINLMGPTASGKTALACELYERGHFELISVDSALIYKDMDIGTAKPTKEELALY
CCCCCEEEECCCCCCCCCEEEHHHHHCCCEEEEEECCCCEEECCCCCCCCCCHHHHHHH
PHHLIDIITPLEVYSAAQFVEDATQLIDQIHMKGKTPILVGGTMLYFKSLLEGLSTNLPS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHCCCCC
ADAEVRAAIEKQAEQEGWQSVYDELARVDPVAGARFKVSDRQRIIRAIEVYRLTGQPITK
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCHHH
LQAEQPKNVPYRYTFHNYALLPDRIELHKRIEQRLFKMWSIGFLNEVESLIKKYDLNDNL
HCCCCCCCCCEEEEECCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PSMRSVGYRQALDFLLKSDMSHNNRREMEDKALFATRQLAKRQYTWLRSLQETHYFKTYL
CHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TIKQAQEDLRNSYG
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA