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The map label for this gene is glcB [H]

Identifier: 50085427

GI number: 50085427

Start: 2298287

End: 2300449

Strand: Reverse

Name: glcB [H]

Synonym: ACIAD2335

Alternate gene names: 50085427

Gene position: 2300449-2298287 (Counterclockwise)

Preceding gene: 50085428

Following gene: 50085426

Centisome position: 63.93

GC content: 40.59

Gene sequence:

>2163_bases
ATGACTGCACGTATTCAAAAAGGCAAGTTAGCGATTGCTAAAGAACTTTACGATTTTATTGAGAATGAAGCTTTATCTGC
TTCTGGTTTGGACAGTGAAACTTACTGGAAAAACTTTGAGCAAGTCGTTGTCGATCTTAGCCCTAAAAACAAGGCACTGC
TTGCGAAGCGTGATGAGTTACAGGCTAAAATTGATGAATGGCATCGTAACAATACATTTGAATTAGAAGCTTACAAAGCT
TTCCTTAAAGAAATTGGCTACTTATTACCAGAAGTAGAAGATTTTCAAATCACTACACAAAATGTAGATGAAGAAATTGC
ATTACTTGCAGGGCCACAATTGGTTGTACCTGTACGCAATGCACGTTATTCACTGAATGCAGCAAATGCACGTTGGGGAT
CACTCTATGACGCATTATACGGCTTCGATGTTATTCCAGAAGATGGTGGTGCTGAAAAAGGCAAGGGATATAATCCTGTA
CGTGGTGCCAAAGTTATTGCATTTGCTAAAAACTTTTTAGATGAAACTTTTCCATTGGCTCAAGGGTCGCATAAAAATGC
TGTTCAATATTCAGTTGAACAAAATGCACTGGTTATCACATTAGATAATGGTGAAAAAACCTCATTGGCACATGAAGCAT
TATTTGTCGGTTATAACGGCGAAGACAGCGCACCTTCTGAAATCGTGCTTAAACACAATGGTCTTCATGTCATTATTGAA
ATTGATGCTAATAGTCCAATTGGCAAGACAGATCCAGCAAGTGTAAAAGACTTAGTACTTGAAGCTGCAATCACGACAAT
TCAAGATCTTGAAGATTCTGTTGCAGCGGTAGATGCAGAAGAAAAAGTTGAAGGGTATCGTAACTGGCTTGGATTGATGC
AGGGAACTCTTGAAGAGTCGATTGAGAAAAATGGTAAAACCGTTACTCGCAAACTAAGTCAGGATCGTACCTGTAAAAAT
CTGATTGGTGGAACCACGACCTTGCACGGTCGTTCATTGATGCTACTACGTAATGTTGGGCATTTAATGACTAATCCTGC
AATTTTAGTCGACGGTGAAGAAATCTACGAAGGTATTATGGACGCGCTGGTTACGTCATTGCTTTCAATTGTAGATATTA
CTGGCAAAAATGAAGTTAAAAACTCTCGTAAAGGTTCGATGTATATTGTTAAACCAAAAATGCATGGACCAGAAGAAGTA
GCATTTACTGTCGAGTTATTTGAACGTGTAGAAAAAGCACTGGGCTTACCAGAGAAATCACTCAAAGTCGGCATTATGGA
TGAAGAGCGTCGTACTTCTGTCAATTTGAAAAACTGTATTGCTCAGGCAAAAGATCGCACCATCTTTATTAATACAGGAT
TTATGGATCGTACAGGTGATGAAATTCATACCTTTATGGAAGCTGGTCCATTTGTGTGTAAAGGTGAGATTAAAGGTCAA
AAATGGTTCCCAGCTTACGAAAATCGCAACGTAATGATTGGACTGCAAACGGGTTTACGTGGTAAAGCGCAAATTGGTAA
AGGTATGTGGCCTAAACCTGATATGTTGCTTGATATGTATAAAACCAAGATTGAACATCCAGAAGCGGGTGCATCATGTG
CATGGGTACCATCTCCATCTGGTGCTGTAATTCACGCAATTCATTATCACCAATGCAATGTTGCAGAGCGCCAGCAAGAA
TTGCTCAGTACACAAGCACTGTCACTAGATGATCTGTTAACACCTCCTTTGGCTGCTGATACCAACTGGTCTGCTGAAGA
AATTACCAAAGAGCTGGAAAATAACTGCCAAGGGATTTTGGGTTACGTGGTGCGTTGGGTTGATCTAGGTGTGGGTTGTT
CAAAAGTACCAGATATTAATGATGTCGGACTCATGGAAGACCGTGCGACACTGCGTATTTCTTCTCAGCATGTAGCAAAC
TGGTTACGTCACGGTATCGTAAGCAAAGAGCAGGTTGAGGAAGTGCTAAAACGTATGGCCAAAATTGTTGATGAGCAAAA
TGCCAATGATCCAGCATATACCCCGATGTCTCCTGGTTTTGATGGGATTGCGTTTCAGGCAGCATCTGATCTCATCTTTA
AGGGCGGTGAACAACCATCGGGTTATACTGAGCCATTATTACATGCGGCACGTTTGAAGTTAAAAGGATATACGGGAGAC
TAA

Upstream 100 bases:

>100_bases
GAAATGTTTGTTATTTCACAAAGTGATTCTACTATTCAGGATGGTATAAATGGTTATACTAGAATCTCTTGTATAAAAAG
TAGCAATATCAGGAAAGACA

Downstream 100 bases:

>100_bases
CTCGTAATTTCAAAAAAACCGCAGTCTAGATTGCGGTTTTTTTATGCGCTAAATGAATTAGGAAGAATTTAATGGACAAT
AGAATCTATAATAATAAATA

Product: malate synthase G

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 720; Mature: 719

Protein sequence:

>720_residues
MTARIQKGKLAIAKELYDFIENEALSASGLDSETYWKNFEQVVVDLSPKNKALLAKRDELQAKIDEWHRNNTFELEAYKA
FLKEIGYLLPEVEDFQITTQNVDEEIALLAGPQLVVPVRNARYSLNAANARWGSLYDALYGFDVIPEDGGAEKGKGYNPV
RGAKVIAFAKNFLDETFPLAQGSHKNAVQYSVEQNALVITLDNGEKTSLAHEALFVGYNGEDSAPSEIVLKHNGLHVIIE
IDANSPIGKTDPASVKDLVLEAAITTIQDLEDSVAAVDAEEKVEGYRNWLGLMQGTLEESIEKNGKTVTRKLSQDRTCKN
LIGGTTTLHGRSLMLLRNVGHLMTNPAILVDGEEIYEGIMDALVTSLLSIVDITGKNEVKNSRKGSMYIVKPKMHGPEEV
AFTVELFERVEKALGLPEKSLKVGIMDEERRTSVNLKNCIAQAKDRTIFINTGFMDRTGDEIHTFMEAGPFVCKGEIKGQ
KWFPAYENRNVMIGLQTGLRGKAQIGKGMWPKPDMLLDMYKTKIEHPEAGASCAWVPSPSGAVIHAIHYHQCNVAERQQE
LLSTQALSLDDLLTPPLAADTNWSAEEITKELENNCQGILGYVVRWVDLGVGCSKVPDINDVGLMEDRATLRISSQHVAN
WLRHGIVSKEQVEEVLKRMAKIVDEQNANDPAYTPMSPGFDGIAFQAASDLIFKGGEQPSGYTEPLLHAARLKLKGYTGD

Sequences:

>Translated_720_residues
MTARIQKGKLAIAKELYDFIENEALSASGLDSETYWKNFEQVVVDLSPKNKALLAKRDELQAKIDEWHRNNTFELEAYKA
FLKEIGYLLPEVEDFQITTQNVDEEIALLAGPQLVVPVRNARYSLNAANARWGSLYDALYGFDVIPEDGGAEKGKGYNPV
RGAKVIAFAKNFLDETFPLAQGSHKNAVQYSVEQNALVITLDNGEKTSLAHEALFVGYNGEDSAPSEIVLKHNGLHVIIE
IDANSPIGKTDPASVKDLVLEAAITTIQDLEDSVAAVDAEEKVEGYRNWLGLMQGTLEESIEKNGKTVTRKLSQDRTCKN
LIGGTTTLHGRSLMLLRNVGHLMTNPAILVDGEEIYEGIMDALVTSLLSIVDITGKNEVKNSRKGSMYIVKPKMHGPEEV
AFTVELFERVEKALGLPEKSLKVGIMDEERRTSVNLKNCIAQAKDRTIFINTGFMDRTGDEIHTFMEAGPFVCKGEIKGQ
KWFPAYENRNVMIGLQTGLRGKAQIGKGMWPKPDMLLDMYKTKIEHPEAGASCAWVPSPSGAVIHAIHYHQCNVAERQQE
LLSTQALSLDDLLTPPLAADTNWSAEEITKELENNCQGILGYVVRWVDLGVGCSKVPDINDVGLMEDRATLRISSQHVAN
WLRHGIVSKEQVEEVLKRMAKIVDEQNANDPAYTPMSPGFDGIAFQAASDLIFKGGEQPSGYTEPLLHAARLKLKGYTGD
>Mature_719_residues
TARIQKGKLAIAKELYDFIENEALSASGLDSETYWKNFEQVVVDLSPKNKALLAKRDELQAKIDEWHRNNTFELEAYKAF
LKEIGYLLPEVEDFQITTQNVDEEIALLAGPQLVVPVRNARYSLNAANARWGSLYDALYGFDVIPEDGGAEKGKGYNPVR
GAKVIAFAKNFLDETFPLAQGSHKNAVQYSVEQNALVITLDNGEKTSLAHEALFVGYNGEDSAPSEIVLKHNGLHVIIEI
DANSPIGKTDPASVKDLVLEAAITTIQDLEDSVAAVDAEEKVEGYRNWLGLMQGTLEESIEKNGKTVTRKLSQDRTCKNL
IGGTTTLHGRSLMLLRNVGHLMTNPAILVDGEEIYEGIMDALVTSLLSIVDITGKNEVKNSRKGSMYIVKPKMHGPEEVA
FTVELFERVEKALGLPEKSLKVGIMDEERRTSVNLKNCIAQAKDRTIFINTGFMDRTGDEIHTFMEAGPFVCKGEIKGQK
WFPAYENRNVMIGLQTGLRGKAQIGKGMWPKPDMLLDMYKTKIEHPEAGASCAWVPSPSGAVIHAIHYHQCNVAERQQEL
LSTQALSLDDLLTPPLAADTNWSAEEITKELENNCQGILGYVVRWVDLGVGCSKVPDINDVGLMEDRATLRISSQHVANW
LRHGIVSKEQVEEVLKRMAKIVDEQNANDPAYTPMSPGFDGIAFQAASDLIFKGGEQPSGYTEPLLHAARLKLKGYTGD

Specific function: Accounts For Almost The Entire Malate-Synthesizing Activity In Cells Metabolizing Glyoxylate. [C]

COG id: COG2225

COG function: function code C; Malate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malate synthase family. GlcB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789348, Length=725, Percent_Identity=56.9655172413793, Blast_Score=855, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011076
- InterPro:   IPR001465
- InterPro:   IPR006253 [H]

Pfam domain/function: PF01274 Malate_synthase [H]

EC number: =2.3.3.9 [H]

Molecular weight: Translated: 79529; Mature: 79398

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00589 PTS_HPR_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARIQKGKLAIAKELYDFIENEALSASGLDSETYWKNFEQVVVDLSPKNKALLAKRDEL
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHEEEECCCCCCEEHEHHHHH
QAKIDEWHRNNTFELEAYKAFLKEIGYLLPEVEDFQITTQNVDEEIALLAGPQLVVPVRN
HHHHHHHHCCCCEEHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHEEECCEEEEEECC
ARYSLNAANARWGSLYDALYGFDVIPEDGGAEKGKGYNPVRGAKVIAFAKNFLDETFPLA
CEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
QGSHKNAVQYSVEQNALVITLDNGEKTSLAHEALFVGYNGEDSAPSEIVLKHNGLHVIIE
CCCCCCCEEEEECCCEEEEEECCCCCCHHEEEEEEEEECCCCCCCCEEEEEECCEEEEEE
IDANSPIGKTDPASVKDLVLEAAITTIQDLEDSVAAVDAEEKVEGYRNWLGLMQGTLEES
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
IEKNGKTVTRKLSQDRTCKNLIGGTTTLHGRSLMLLRNVGHLMTNPAILVDGEEIYEGIM
HHHCCHHHHHHHHCCHHHHHHHCCCEEECCCHHHHHHHHHHHHCCCEEEECCHHHHHHHH
DALVTSLLSIVDITGKNEVKNSRKGSMYIVKPKMHGPEEVAFTVELFERVEKALGLPEKS
HHHHHHHHHHHHCCCCHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHH
LKVGIMDEERRTSVNLKNCIAQAKDRTIFINTGFMDRTGDEIHTFMEAGPFVCKGEIKGQ
EEEEECCCHHHHCCCHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHCCCCEEEECCCCC
KWFPAYENRNVMIGLQTGLRGKAQIGKGMWPKPDMLLDMYKTKIEHPEAGASCAWVPSPS
CCCCCCCCCCEEEEECCCCCCHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCC
GAVIHAIHYHQCNVAERQQELLSTQALSLDDLLTPPLAADTNWSAEEITKELENNCQGIL
CCEEEEEEECCCCHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
GYVVRWVDLGVGCSKVPDINDVGLMEDRATLRISSQHVANWLRHGIVSKEQVEEVLKRMA
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHCCCCHHHHHHHHHHHH
KIVDEQNANDPAYTPMSPGFDGIAFQAASDLIFKGGEQPSGYTEPLLHAARLKLKGYTGD
HHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHEECCCCCC
>Mature Secondary Structure 
TARIQKGKLAIAKELYDFIENEALSASGLDSETYWKNFEQVVVDLSPKNKALLAKRDEL
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHEEEECCCCCCEEHEHHHHH
QAKIDEWHRNNTFELEAYKAFLKEIGYLLPEVEDFQITTQNVDEEIALLAGPQLVVPVRN
HHHHHHHHCCCCEEHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHEEECCEEEEEECC
ARYSLNAANARWGSLYDALYGFDVIPEDGGAEKGKGYNPVRGAKVIAFAKNFLDETFPLA
CEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
QGSHKNAVQYSVEQNALVITLDNGEKTSLAHEALFVGYNGEDSAPSEIVLKHNGLHVIIE
CCCCCCCEEEEECCCEEEEEECCCCCCHHEEEEEEEEECCCCCCCCEEEEEECCEEEEEE
IDANSPIGKTDPASVKDLVLEAAITTIQDLEDSVAAVDAEEKVEGYRNWLGLMQGTLEES
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
IEKNGKTVTRKLSQDRTCKNLIGGTTTLHGRSLMLLRNVGHLMTNPAILVDGEEIYEGIM
HHHCCHHHHHHHHCCHHHHHHHCCCEEECCCHHHHHHHHHHHHCCCEEEECCHHHHHHHH
DALVTSLLSIVDITGKNEVKNSRKGSMYIVKPKMHGPEEVAFTVELFERVEKALGLPEKS
HHHHHHHHHHHHCCCCHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHH
LKVGIMDEERRTSVNLKNCIAQAKDRTIFINTGFMDRTGDEIHTFMEAGPFVCKGEIKGQ
EEEEECCCHHHHCCCHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHCCCCEEEECCCCC
KWFPAYENRNVMIGLQTGLRGKAQIGKGMWPKPDMLLDMYKTKIEHPEAGASCAWVPSPS
CCCCCCCCCCEEEEECCCCCCHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCC
GAVIHAIHYHQCNVAERQQELLSTQALSLDDLLTPPLAADTNWSAEEITKELENNCQGIL
CCEEEEEEECCCCHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
GYVVRWVDLGVGCSKVPDINDVGLMEDRATLRISSQHVANWLRHGIVSKEQVEEVLKRMA
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHCCCCHHHHHHHHHHHH
KIVDEQNANDPAYTPMSPGFDGIAFQAASDLIFKGGEQPSGYTEPLLHAARLKLKGYTGD
HHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]