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The map label for this gene is proP [H]

Identifier: 50085380

GI number: 50085380

Start: 2250672

End: 2252186

Strand: Direct

Name: proP [H]

Synonym: ACIAD2280

Alternate gene names: 50085380

Gene position: 2250672-2252186 (Clockwise)

Preceding gene: 50085378

Following gene: 50085382

Centisome position: 62.54

GC content: 40.46

Gene sequence:

>1515_bases
ATGAGTTTACTTACAACAGGAAATCAGTCATCTATGTCTAAAAAAACAATAAAACCTCTTGAAATCGATGATGTTGAAAT
TGTCGATTACGACAAGACCAAAAAAGCAATTAAAGCCGCAGCACTTGGTAATGCCATCGAATGGTTCGATTTTGGTGTAT
ATGGTTATGTTGCGTATGTTTTGGGAAAAGTATTTTTTCCAAACTCTTCCCCAAGTGTGCAAATGATTGCGGCACTTGCA
ACCTTTTCGATTCCTTTTATTTTTAGACCCTTGGGAGGATTATTTTTTGGACGGCTGGGTGATAAATATGGGCGACAAAA
AGTTTTAGCCATCACGATTATCATTATGTCGGTCAGTACTTTTGGTATTGGTTTGATTCCTTCGTATGAAACCATCGGAA
TTTGGGCGCCTCTTCTTTTATTAATTGTGAAAATCGCGCAAGGCTTTTCTGTAGGTGGAGAGTATGCAGGAGCAGCAATT
TTTGTTGCAGAGTATTCACCAGACCGTAAGCGTGGCTTTATGGGAAGCTGGCTGGATTTCGGCTCGATTTTAGGTTTTGT
GGTGGGTGCAGGAATTGTATCACTGATCAGTTTTTCTGTAGGCGAAGCAGCGTTTATGGAATGGGGATGGCGTATTCCAT
TTTATTTGGCACTGCCGCTCGGTTTAATTGGTCTATACTTACGCCATTCCCTTGATGAGACACCTACTTTTCAGGCACAT
AATGAAAGTCAGGCACAACTGAAAAAACCTAAAAAAATCAGTTTTTCACAGATTCTACAACAACATAAACGAAGCCTTCT
GGTTTGCGTTGGACTCGTGATCTGTACCAACGTCACTTACTATATGCTACTGACTTATCTACCAAGTTATTTCTCACATA
ATTTGGGATATTCAGAGGATCACGGTGTTTTAATTATTATTGCAGTGATGGTGGGTATGCTCTTTGTACAACCATTTGTT
GGATTGCTCAGTGACAAGATTGGCCGCCGCCCTTTTGTTTTCGCTGGTAGTATTGCTTTGGTTTTATTTTCGTATCCTGC
TTTTCTTTTACTGGATACCAATAATTCTTATCTGATCTTTTTTGGCTTATTGATATTGGCACTTTCGCTAAATATGCTAA
TAGGTGTTATGGCATCCACTTTGCCAGCCCTGTTTCCTACCGAAATCCGTTACAGCGCATTGGCAATCGCATTTAATATC
TCTGTAGTCATTGCAGGCCTTACCCCAACAGTTACAGCGGCGCTCGTCGAAACCACACAGAATATTATGGTTCCTGCTTA
TTACTTGATGATCTGTGGTGTCTTCGGCATTATCACCGCAATTTATCTAAAAGAAACGGCAAACTTACCGTTGAAAGGTG
CAACGCCAATGGCTGGCGATTCTGAAGAAGCGGAGGAACTGCTTGAACAGTATCATGAAAATATTGAACAGAAGATCGAA
CATCTCGATGAGCAGATTGAAGCATTACAGGAGAAACGCGATAAACTTGCAAAACAACATCCTGATATTGAATGA

Upstream 100 bases:

>100_bases
TTTAAGTCTTTATAGAATAGTTTCGTGTGTAGTTTCTCATGATTAAGTTCACTTTTTCTCATATTTTATGAACTTAACTT
TGTAGATATCTAGACGCAAG

Downstream 100 bases:

>100_bases
TTATTTATAAAAGTGGGTAAAATATACCCACTTTTATTTGCAAGATCTCTTATTTTTACAACACTCAATATATTTTAACT
TTGATGAAACTAATTAAGTT

Product: proline/glycine betaine transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 504; Mature: 503

Protein sequence:

>504_residues
MSLLTTGNQSSMSKKTIKPLEIDDVEIVDYDKTKKAIKAAALGNAIEWFDFGVYGYVAYVLGKVFFPNSSPSVQMIAALA
TFSIPFIFRPLGGLFFGRLGDKYGRQKVLAITIIIMSVSTFGIGLIPSYETIGIWAPLLLLIVKIAQGFSVGGEYAGAAI
FVAEYSPDRKRGFMGSWLDFGSILGFVVGAGIVSLISFSVGEAAFMEWGWRIPFYLALPLGLIGLYLRHSLDETPTFQAH
NESQAQLKKPKKISFSQILQQHKRSLLVCVGLVICTNVTYYMLLTYLPSYFSHNLGYSEDHGVLIIIAVMVGMLFVQPFV
GLLSDKIGRRPFVFAGSIALVLFSYPAFLLLDTNNSYLIFFGLLILALSLNMLIGVMASTLPALFPTEIRYSALAIAFNI
SVVIAGLTPTVTAALVETTQNIMVPAYYLMICGVFGIITAIYLKETANLPLKGATPMAGDSEEAEELLEQYHENIEQKIE
HLDEQIEALQEKRDKLAKQHPDIE

Sequences:

>Translated_504_residues
MSLLTTGNQSSMSKKTIKPLEIDDVEIVDYDKTKKAIKAAALGNAIEWFDFGVYGYVAYVLGKVFFPNSSPSVQMIAALA
TFSIPFIFRPLGGLFFGRLGDKYGRQKVLAITIIIMSVSTFGIGLIPSYETIGIWAPLLLLIVKIAQGFSVGGEYAGAAI
FVAEYSPDRKRGFMGSWLDFGSILGFVVGAGIVSLISFSVGEAAFMEWGWRIPFYLALPLGLIGLYLRHSLDETPTFQAH
NESQAQLKKPKKISFSQILQQHKRSLLVCVGLVICTNVTYYMLLTYLPSYFSHNLGYSEDHGVLIIIAVMVGMLFVQPFV
GLLSDKIGRRPFVFAGSIALVLFSYPAFLLLDTNNSYLIFFGLLILALSLNMLIGVMASTLPALFPTEIRYSALAIAFNI
SVVIAGLTPTVTAALVETTQNIMVPAYYLMICGVFGIITAIYLKETANLPLKGATPMAGDSEEAEELLEQYHENIEQKIE
HLDEQIEALQEKRDKLAKQHPDIE
>Mature_503_residues
SLLTTGNQSSMSKKTIKPLEIDDVEIVDYDKTKKAIKAAALGNAIEWFDFGVYGYVAYVLGKVFFPNSSPSVQMIAALAT
FSIPFIFRPLGGLFFGRLGDKYGRQKVLAITIIIMSVSTFGIGLIPSYETIGIWAPLLLLIVKIAQGFSVGGEYAGAAIF
VAEYSPDRKRGFMGSWLDFGSILGFVVGAGIVSLISFSVGEAAFMEWGWRIPFYLALPLGLIGLYLRHSLDETPTFQAHN
ESQAQLKKPKKISFSQILQQHKRSLLVCVGLVICTNVTYYMLLTYLPSYFSHNLGYSEDHGVLIIIAVMVGMLFVQPFVG
LLSDKIGRRPFVFAGSIALVLFSYPAFLLLDTNNSYLIFFGLLILALSLNMLIGVMASTLPALFPTEIRYSALAIAFNIS
VVIAGLTPTVTAALVETTQNIMVPAYYLMICGVFGIITAIYLKETANLPLKGATPMAGDSEEAEELLEQYHENIEQKIEH
LDEQIEALQEKRDKLAKQHPDIE

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=498, Percent_Identity=68.4738955823293, Blast_Score=719, Evalue=0.0,
Organism=Escherichia coli, GI1788292, Length=429, Percent_Identity=32.6340326340326, Blast_Score=252, Evalue=5e-68,
Organism=Escherichia coli, GI1789941, Length=432, Percent_Identity=33.3333333333333, Blast_Score=212, Evalue=4e-56,
Organism=Escherichia coli, GI1788942, Length=399, Percent_Identity=31.5789473684211, Blast_Score=208, Evalue=7e-55,
Organism=Escherichia coli, GI87082404, Length=388, Percent_Identity=26.2886597938144, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87082231, Length=436, Percent_Identity=22.7064220183486, Blast_Score=85, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 55451; Mature: 55320

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLTTGNQSSMSKKTIKPLEIDDVEIVDYDKTKKAIKAAALGNAIEWFDFGVYGYVAYV
CCCCCCCCCHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LGKVFFPNSSPSVQMIAALATFSIPFIFRPLGGLFFGRLGDKYGRQKVLAITIIIMSVST
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
FGIGLIPSYETIGIWAPLLLLIVKIAQGFSVGGEYAGAAIFVAEYSPDRKRGFMGSWLDF
HCEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHCCCCCHHHHH
GSILGFVVGAGIVSLISFSVGEAAFMEWGWRIPFYLALPLGLIGLYLRHSLDETPTFQAH
HHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
NESQAQLKKPKKISFSQILQQHKRSLLVCVGLVICTNVTYYMLLTYLPSYFSHNLGYSED
CCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
HGVLIIIAVMVGMLFVQPFVGLLSDKIGRRPFVFAGSIALVLFSYPAFLLLDTNNSYLIF
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCEEEEEECCCCCHHH
FGLLILALSLNMLIGVMASTLPALFPTEIRYSALAIAFNISVVIAGLTPTVTAALVETTQ
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEEEEEEEECCCHHHHHHHHHHHH
NIMVPAYYLMICGVFGIITAIYLKETANLPLKGATPMAGDSEEAEELLEQYHENIEQKIE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
HLDEQIEALQEKRDKLAKQHPDIE
HHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SLLTTGNQSSMSKKTIKPLEIDDVEIVDYDKTKKAIKAAALGNAIEWFDFGVYGYVAYV
CCCCCCCCHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LGKVFFPNSSPSVQMIAALATFSIPFIFRPLGGLFFGRLGDKYGRQKVLAITIIIMSVST
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
FGIGLIPSYETIGIWAPLLLLIVKIAQGFSVGGEYAGAAIFVAEYSPDRKRGFMGSWLDF
HCEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHCCCCCHHHHH
GSILGFVVGAGIVSLISFSVGEAAFMEWGWRIPFYLALPLGLIGLYLRHSLDETPTFQAH
HHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
NESQAQLKKPKKISFSQILQQHKRSLLVCVGLVICTNVTYYMLLTYLPSYFSHNLGYSED
CCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
HGVLIIIAVMVGMLFVQPFVGLLSDKIGRRPFVFAGSIALVLFSYPAFLLLDTNNSYLIF
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCEEEEEECCCCCHHH
FGLLILALSLNMLIGVMASTLPALFPTEIRYSALAIAFNISVVIAGLTPTVTAALVETTQ
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEEEEEEEECCCHHHHHHHHHHHH
NIMVPAYYLMICGVFGIITAIYLKETANLPLKGATPMAGDSEEAEELLEQYHENIEQKIE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
HLDEQIEALQEKRDKLAKQHPDIE
HHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]