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The map label for this gene is pucI [H]

Identifier: 50085352

GI number: 50085352

Start: 2217862

End: 2219328

Strand: Direct

Name: pucI [H]

Synonym: ACIAD2247

Alternate gene names: 50085352

Gene position: 2217862-2219328 (Clockwise)

Preceding gene: 50085351

Following gene: 50085353

Centisome position: 61.63

GC content: 39.2

Gene sequence:

>1467_bases
GTGACTAACATGCAACATGAATCTGAAGCCCTAATCAAACCCGATTACCACCCTAAATTAACCAACTCTGACCTAGCACC
TTTAAAAAAACAAACGTGGACATCGTATAACATCTTTGCCTTTTGGATGTCTGATGTACACAGTGTTGGTGGCTATGTAA
CTGCTGGAAGCTTATTCGCACTCGGCCTCAATAGTTGGCAGGTATTGGTATCATTACTTATCGGCATTGTGATTGTACAA
TTTTTTGCCAATATGATTGCAAAACCAAGCCAAAAAACCAGTACACCTTTTCCGGTGATCTGTCGCGCAACCTTTGGTGT
TTTTGGTGCCAATATTCCTGCTGTGATTCGTGGAATTATTGCTGTTGCATGGTATGGCATTCAGACTTATCTGGCTTCAA
GCGCATTTATTATTATCATTTTAAAGTTTTGGCCTGAAATGACAGCATATGCTGATGTCAAACAACATTCATTTTTAGGA
TTGTCTTATCTGGGATGGCTAGGTTTTATGCTGCTCTGGGTACTTCAGGCAGTCGTATTCTGGTCAGGCATGAATAGTAT
TCGCAAGTTTATCGATTGGGCTGGTCCTGCGGTTTATGTTGTGATGTTTGCCATGGCAGTGTGGTTGGTCTATGAGGCTG
GCTGGAGTAATATCAACATGAATCTAGGCGGCGTCAAATACGACGGCATGGATGTTATCCCCGTTATGATTGGTGCCATT
GCATTAGTTGTATCTTATTTTTCTGGTCCTGTACTTAATTTTGGCGATTTCTCACGCTATGGTAAAAGCTTTGAAGCAGT
TAAATTTGGTAATTTCTTAGGCTTACCTGTCAACTTTCTAGCATTCTCAGTATTAACAGTAGTCTGTATCGCAGCGACTT
TGCCTATTTATGGTAAGTTAATTACAGATCCTGTAGAAATGGTTGGACAACTAAATAATACTTTTGTGGTAGTTCTTGGT
TGTCTCACGCTCATGATTGCAACGGTTGGCATTAACATTGTGGCTAACTTTGTATCACCTGCTTTTGATTTTTCAAATGT
TTCACCCAATAAAATCAGTTGGCGAATGGGAGGTATGATTGCAGCAGTGGGTTCAATCTTTATTACGCCATGGAATTTAT
TTAACAACCCTCAGGTGATTCACTACACCATTGATGTTTTAGGTGCATTAATTGGTCCATTATTTGGTATCTTGATCGCA
GATTATTACATCGTTAAGAAACAAAAAATTGAGGTAGATGAATTATATAGCATTAATCCTCATGGAAAGTACTGGTACCA
AAATGGCTATAATCCAAAAGCTATTGCAGCACTGATTCCCGCAGCAATTATCCCTATTCTTTGTGTATTGCTCCCTCAGC
TGCATATATTGGCAAATTTCTCCTGGTTTATTGGAATGTTTGCAGGTCTGATTATTTACAGTCTGCTCTCTATTAAACAA
CGTGTGAAAATAAAATCGTTGATCTAG

Upstream 100 bases:

>100_bases
CCAATCAAGATCAACTCTCAACTGCTGTTGGTGTTCGTCTGACATTTTAATTCAACTTCAAATGCAATTTTACGATGACC
CTATGACAAAATGAAAGTGA

Downstream 100 bases:

>100_bases
CACACGCTTTACTCAATCAAATCGAAGTAAAGACTTCCATAAACTTATGATTGAAAAATTTGTGGAAGTCGCTTATCGCT
TTAAATTTTATCGTTCAATA

Product: permease

Products: NA

Alternate protein names: Allantoin transport protein [H]

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MTNMQHESEALIKPDYHPKLTNSDLAPLKKQTWTSYNIFAFWMSDVHSVGGYVTAGSLFALGLNSWQVLVSLLIGIVIVQ
FFANMIAKPSQKTSTPFPVICRATFGVFGANIPAVIRGIIAVAWYGIQTYLASSAFIIIILKFWPEMTAYADVKQHSFLG
LSYLGWLGFMLLWVLQAVVFWSGMNSIRKFIDWAGPAVYVVMFAMAVWLVYEAGWSNINMNLGGVKYDGMDVIPVMIGAI
ALVVSYFSGPVLNFGDFSRYGKSFEAVKFGNFLGLPVNFLAFSVLTVVCIAATLPIYGKLITDPVEMVGQLNNTFVVVLG
CLTLMIATVGINIVANFVSPAFDFSNVSPNKISWRMGGMIAAVGSIFITPWNLFNNPQVIHYTIDVLGALIGPLFGILIA
DYYIVKKQKIEVDELYSINPHGKYWYQNGYNPKAIAALIPAAIIPILCVLLPQLHILANFSWFIGMFAGLIIYSLLSIKQ
RVKIKSLI

Sequences:

>Translated_488_residues
MTNMQHESEALIKPDYHPKLTNSDLAPLKKQTWTSYNIFAFWMSDVHSVGGYVTAGSLFALGLNSWQVLVSLLIGIVIVQ
FFANMIAKPSQKTSTPFPVICRATFGVFGANIPAVIRGIIAVAWYGIQTYLASSAFIIIILKFWPEMTAYADVKQHSFLG
LSYLGWLGFMLLWVLQAVVFWSGMNSIRKFIDWAGPAVYVVMFAMAVWLVYEAGWSNINMNLGGVKYDGMDVIPVMIGAI
ALVVSYFSGPVLNFGDFSRYGKSFEAVKFGNFLGLPVNFLAFSVLTVVCIAATLPIYGKLITDPVEMVGQLNNTFVVVLG
CLTLMIATVGINIVANFVSPAFDFSNVSPNKISWRMGGMIAAVGSIFITPWNLFNNPQVIHYTIDVLGALIGPLFGILIA
DYYIVKKQKIEVDELYSINPHGKYWYQNGYNPKAIAALIPAAIIPILCVLLPQLHILANFSWFIGMFAGLIIYSLLSIKQ
RVKIKSLI
>Mature_487_residues
TNMQHESEALIKPDYHPKLTNSDLAPLKKQTWTSYNIFAFWMSDVHSVGGYVTAGSLFALGLNSWQVLVSLLIGIVIVQF
FANMIAKPSQKTSTPFPVICRATFGVFGANIPAVIRGIIAVAWYGIQTYLASSAFIIIILKFWPEMTAYADVKQHSFLGL
SYLGWLGFMLLWVLQAVVFWSGMNSIRKFIDWAGPAVYVVMFAMAVWLVYEAGWSNINMNLGGVKYDGMDVIPVMIGAIA
LVVSYFSGPVLNFGDFSRYGKSFEAVKFGNFLGLPVNFLAFSVLTVVCIAATLPIYGKLITDPVEMVGQLNNTFVVVLGC
LTLMIATVGINIVANFVSPAFDFSNVSPNKISWRMGGMIAAVGSIFITPWNLFNNPQVIHYTIDVLGALIGPLFGILIAD
YYIVKKQKIEVDELYSINPHGKYWYQNGYNPKAIAALIPAAIIPILCVLLPQLHILANFSWFIGMFAGLIIYSLLSIKQR
VKIKSLI

Specific function: Transport of allantoin [H]

COG id: COG1953

COG function: function code FH; Cytosine/uracil/thiamine/allantoin permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family [H]

Homologues:

Organism=Escherichia coli, GI87081749, Length=497, Percent_Identity=27.9678068410463, Blast_Score=216, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6319495, Length=439, Percent_Identity=23.4624145785877, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6322219, Length=431, Percent_Identity=21.8097447795824, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6319429, Length=436, Percent_Identity=23.394495412844, Blast_Score=130, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6324766, Length=442, Percent_Identity=21.4932126696833, Blast_Score=102, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6323266, Length=435, Percent_Identity=22.0689655172414, Blast_Score=100, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6324645, Length=438, Percent_Identity=21.2328767123288, Blast_Score=96, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001248
- InterPro:   IPR012681 [H]

Pfam domain/function: PF02133 Transp_cyt_pur [H]

EC number: NA

Molecular weight: Translated: 53924; Mature: 53792

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHCC
>Mature Secondary Structure 
TNMQHESEALIKPDYHPKLTNSDLAPLKKQTWTSYNIFAFWMSDVHSVGGYVTAGSLFA
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHCCEEECCHHHH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HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 11344136 [H]