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The map label for this gene is dctA

Identifier: 50085335

GI number: 50085335

Start: 2199881

End: 2201227

Strand: Reverse

Name: dctA

Synonym: ACIAD2227

Alternate gene names: 50085335

Gene position: 2201227-2199881 (Counterclockwise)

Preceding gene: 50085337

Following gene: 50085334

Centisome position: 61.17

GC content: 40.46

Gene sequence:

>1347_bases
ATGGCTAAAAAACCAATTTATAAATCGCTCTATTTTCAAGTTATTGTTGCAATTATCGCAGGTATCTTGGTCGGGCATTT
TTATCCAAGTACCACACATGTTGTCGATGGTGTCAAAGAGCATATTCCTGGTTTGGGTGAGCAGCTTAAACCACTTGGTG
ATGCGTTCATTCGTCTGATCAAAATGATTATTGCACCAGTGATTTTCTGTACCGTGGTGAGCGGAATCGCTGGTATGGAA
AGCATGAAGTCTGTGGGTAAGACAGGCGGTGTTGCTCTATTATATTTTGAGGTCGTTTCAACGATTGCATTGCTGATTGG
CTTATTGGTCATCAATATTGCCAAACCAGGCGTTGGCATGAATGTAGATCCTGCATCGCTAGATACTTCTGGTATTGCAA
AATATGTTGCATCGGGCCAATCACAATCCACGATTGAATTCTTAATGCATATCATCCCAGAAACTGTAGTGGGTGCATTT
GCAAATGGTGAGATTCTTCAGGTTCTGTTATTTGCCATTATGTTTGGTTTTGCTCTACATAAACTTGGTGATGCAGGCCG
TCCAGTGCTTAAGTTTATCGATCAGATTTCTCATGTTTTCTTTAACATTGTAAATATGATCATGAAACTTGCTCCGATTG
GCGCATTTGGTGCTATGGCATTTACCATTGGTAAATATGGTGTAGGTTCTTTAGTACAATTAGGTCAGTTAATCATTTGT
TTCTATGTGACCTGTTTACTGTTTATTTTCATTGTATTGGGAACCATCAGTCGTATCTGTGGATTCAGTATTCTGAAAAT
GATTCGTTTAATTCGTGAAGAGTTATTGATTGTTCTTGGGACATCATCTTCTGAGTCTGTATTGCCACGTATGCTACGCA
AGCTTGAAATTGCAGGCTGTGAAAAATCGGTCGTCGGTCTGGTTATCCCGACAGGTTATTCATTTAACCTAGATGGTACT
TCGATTTATCTGACGATGGCAGCAATCTTTATTGCACAAGCGACCAATACTCAGTTAGATGTTCAGCATCAAATTACATT
GCTATTGGTCTTGTTGATTTCTTCTAAAGGTGCTGCGGGTGTAACAGGTTCAGGCTTTATCGTGATGGCTGCGACCTTAT
CAGCAGTGGGTCATATTCCTGTTGCAGGTTTGGCATTGATTCTTGGTATTGATCGTTTCATGTCTGAAGCACGTGCATTA
ACTAACCTTACAGGTAATACTTTAGCAACAATTGCAGTTGCCAAGTGGGTGGGTGCATTGGATAAAGAAAAGCTTGATGA
AGCTTTGAATAATCCAGCTGAAGTGGATCGCAAAATGCTTGAAGCGGATCGTCCTGCACATGCATAA

Upstream 100 bases:

>100_bases
GTTGTTTCCTGCAAAGGACTAAAGGGCTGTCTATATCAAGAATATAGATTTTTCATTTGCATATTTCTAAGGATTTAGAA
ATACAGATTGAAGTAAAGTT

Downstream 100 bases:

>100_bases
CATGTGATTGGAATATTTATATTAAAAAAGGAGGCTTCGGCCTCCTTTTTTTATCCGACTCGTGCGTTCATGTGTTTTGA
TCATTGTTGACATGACAGGG

Product: C4-dicarboxylate transporter DctA

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MAKKPIYKSLYFQVIVAIIAGILVGHFYPSTTHVVDGVKEHIPGLGEQLKPLGDAFIRLIKMIIAPVIFCTVVSGIAGME
SMKSVGKTGGVALLYFEVVSTIALLIGLLVINIAKPGVGMNVDPASLDTSGIAKYVASGQSQSTIEFLMHIIPETVVGAF
ANGEILQVLLFAIMFGFALHKLGDAGRPVLKFIDQISHVFFNIVNMIMKLAPIGAFGAMAFTIGKYGVGSLVQLGQLIIC
FYVTCLLFIFIVLGTISRICGFSILKMIRLIREELLIVLGTSSSESVLPRMLRKLEIAGCEKSVVGLVIPTGYSFNLDGT
SIYLTMAAIFIAQATNTQLDVQHQITLLLVLLISSKGAAGVTGSGFIVMAATLSAVGHIPVAGLALILGIDRFMSEARAL
TNLTGNTLATIAVAKWVGALDKEKLDEALNNPAEVDRKMLEADRPAHA

Sequences:

>Translated_448_residues
MAKKPIYKSLYFQVIVAIIAGILVGHFYPSTTHVVDGVKEHIPGLGEQLKPLGDAFIRLIKMIIAPVIFCTVVSGIAGME
SMKSVGKTGGVALLYFEVVSTIALLIGLLVINIAKPGVGMNVDPASLDTSGIAKYVASGQSQSTIEFLMHIIPETVVGAF
ANGEILQVLLFAIMFGFALHKLGDAGRPVLKFIDQISHVFFNIVNMIMKLAPIGAFGAMAFTIGKYGVGSLVQLGQLIIC
FYVTCLLFIFIVLGTISRICGFSILKMIRLIREELLIVLGTSSSESVLPRMLRKLEIAGCEKSVVGLVIPTGYSFNLDGT
SIYLTMAAIFIAQATNTQLDVQHQITLLLVLLISSKGAAGVTGSGFIVMAATLSAVGHIPVAGLALILGIDRFMSEARAL
TNLTGNTLATIAVAKWVGALDKEKLDEALNNPAEVDRKMLEADRPAHA
>Mature_447_residues
AKKPIYKSLYFQVIVAIIAGILVGHFYPSTTHVVDGVKEHIPGLGEQLKPLGDAFIRLIKMIIAPVIFCTVVSGIAGMES
MKSVGKTGGVALLYFEVVSTIALLIGLLVINIAKPGVGMNVDPASLDTSGIAKYVASGQSQSTIEFLMHIIPETVVGAFA
NGEILQVLLFAIMFGFALHKLGDAGRPVLKFIDQISHVFFNIVNMIMKLAPIGAFGAMAFTIGKYGVGSLVQLGQLIICF
YVTCLLFIFIVLGTISRICGFSILKMIRLIREELLIVLGTSSSESVLPRMLRKLEIAGCEKSVVGLVIPTGYSFNLDGTS
IYLTMAAIFIAQATNTQLDVQHQITLLLVLLISSKGAAGVTGSGFIVMAATLSAVGHIPVAGLALILGIDRFMSEARALT
NLTGNTLATIAVAKWVGALDKEKLDEALNNPAEVDRKMLEADRPAHA

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family

Homologues:

Organism=Homo sapiens, GI21314632, Length=406, Percent_Identity=28.5714285714286, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI169790839, Length=416, Percent_Identity=27.1634615384615, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI40254478, Length=433, Percent_Identity=25.635103926097, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI194239697, Length=428, Percent_Identity=24.2990654205607, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI4827012, Length=436, Percent_Identity=26.1467889908257, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI5032093, Length=400, Percent_Identity=27.75, Blast_Score=112, Evalue=9e-25,
Organism=Homo sapiens, GI66773030, Length=439, Percent_Identity=26.879271070615, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI223468566, Length=272, Percent_Identity=31.25, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI223468564, Length=272, Percent_Identity=31.25, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI262359914, Length=376, Percent_Identity=25.531914893617, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI301601644, Length=149, Percent_Identity=30.2013422818792, Blast_Score=79, Evalue=9e-15,
Organism=Escherichia coli, GI1789947, Length=431, Percent_Identity=70.9976798143852, Blast_Score=629, Evalue=0.0,
Organism=Escherichia coli, GI1790514, Length=427, Percent_Identity=39.1100702576112, Blast_Score=318, Evalue=4e-88,
Organism=Escherichia coli, GI1788024, Length=393, Percent_Identity=25.9541984732824, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789473, Length=329, Percent_Identity=20.9726443768997, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17537407, Length=391, Percent_Identity=26.3427109974425, Blast_Score=131, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI71983099, Length=412, Percent_Identity=25.9708737864078, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI71983106, Length=427, Percent_Identity=25.9953161592506, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI71996953, Length=416, Percent_Identity=24.0384615384615, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI193206505, Length=425, Percent_Identity=24.9411764705882, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI193206654, Length=435, Percent_Identity=23.6781609195402, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17541374, Length=400, Percent_Identity=23.75, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24583025, Length=421, Percent_Identity=24.7030878859857, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI17137668, Length=421, Percent_Identity=24.7030878859857, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24583023, Length=421, Percent_Identity=24.7030878859857, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI281360481, Length=450, Percent_Identity=23.5555555555556, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI281360483, Length=450, Percent_Identity=23.5555555555556, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI17137666, Length=450, Percent_Identity=23.5555555555556, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DCTA_ACIAD (Q6FA90)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_046845.1
- ProteinModelPortal:   Q6FA90
- SMR:   Q6FA90
- GeneID:   2881094
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD2227
- NMPDR:   fig|62977.3.peg.2132
- eggNOG:   COG1301
- HOGENOM:   HBG739804
- OMA:   ACFYITC
- PhylomeDB:   Q6FA90
- ProtClustDB:   PRK01663
- BioCyc:   ASP62977:ACIAD2227-MONOMER
- HAMAP:   MF_01300
- InterPro:   IPR001991
- InterPro:   IPR018107
- PANTHER:   PTHR11958
- PRINTS:   PR00173

Pfam domain/function: PF00375 SDF

EC number: NA

Molecular weight: Translated: 47691; Mature: 47560

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x18db4fd0)-; HASH(0x18602604)-; HASH(0x18736f68)-; HASH(0x19662484)-; HASH(0x184d2264)-; HASH(0x1965f828)-; HASH(0x19634488)-; HASH(0x18b93d6c)-; HASH(0x19542b88)-;

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CHHHHHHHHCCHHHHHHHHHHCCCCCCH
>Mature Secondary Structure 
AKKPIYKSLYFQVIVAIIAGILVGHFYPSTTHVVDGVKEHIPGLGEQLKPLGDAFIRLI
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
KMIIAPVIFCTVVSGIAGMESMKSVGKTGGVALLYFEVVSTIALLIGLLVINIAKPGVGM
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NVDPASLDTSGIAKYVASGQSQSTIEFLMHIIPETVVGAFANGEILQVLLFAIMFGFALH
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
KLGDAGRPVLKFIDQISHVFFNIVNMIMKLAPIGAFGAMAFTIGKYGVGSLVQLGQLIIC
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHH
FYVTCLLFIFIVLGTISRICGFSILKMIRLIREELLIVLGTSSSESVLPRMLRKLEIAGC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHCCC
EKSVVGLVIPTGYSFNLDGTSIYLTMAAIFIAQATNTQLDVQHQITLLLVLLISSKGAAG
CCCCEEEEEECCEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCC
VTGSGFIVMAATLSAVGHIPVAGLALILGIDRFMSEARALTNLTGNTLATIAVAKWVGAL
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
DKEKLDEALNNPAEVDRKMLEADRPAHA
CHHHHHHHHCCHHHHHHHHHHCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA