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The map label for this gene is gcvH

Identifier: 50085018

GI number: 50085018

Start: 1869968

End: 1870342

Strand: Direct

Name: gcvH

Synonym: ACIAD1876

Alternate gene names: 50085018

Gene position: 1869968-1870342 (Clockwise)

Preceding gene: 50085017

Following gene: 50085022

Centisome position: 51.96

GC content: 42.4

Gene sequence:

>375_bases
ATGAATCATCCTTCAGAATTGAAGTATGCAACTACCCATGAATGGGTACGAATTGAAGGTGATCTTATCGTCACTGGAAT
TAGTGATCATGCACAAGATGCCTTAGGCGATCTTGTCTATGTAGAAGTTCCTGAAGTAGGGAGCCATATGACTGCTGGCA
CACAAGCTGGCGTGGTTGAGTCTGTAAAAACAGCTTCAGACATTCATGCACCAGTATCGGGTACAGTGGTTGAAGTCAAT
ACTGCACTGGAAGATGATCCAGACTTTATCAATGACGAACCTTATGGCAAAGGCTGGATTTATAAAATGAAACCAGACAA
CATCGCAGACGTAGATCAACTTCTCTCCAATACCGATTACGAAGCTGGTTTATAA

Upstream 100 bases:

>100_bases
AGTAATTGGCGTAAAGACCCTTACCTATAAACTTTTATTTTGAATCAAAAACCTGTATAAACCTATCAAAATCAATAAAA
TTGAATGTTGAGGAGCTGCC

Downstream 100 bases:

>100_bases
AAGATGTACTCTCCCTTATGATTTGAGGGAGAGTTTTAGCGCTTTAAGCTGATTGGCGTAAAGGACATTGTGCGAGTTGA
TCAGCCATTTCTAAAAGTAA

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 124; Mature: 124

Protein sequence:

>124_residues
MNHPSELKYATTHEWVRIEGDLIVTGISDHAQDALGDLVYVEVPEVGSHMTAGTQAGVVESVKTASDIHAPVSGTVVEVN
TALEDDPDFINDEPYGKGWIYKMKPDNIADVDQLLSNTDYEAGL

Sequences:

>Translated_124_residues
MNHPSELKYATTHEWVRIEGDLIVTGISDHAQDALGDLVYVEVPEVGSHMTAGTQAGVVESVKTASDIHAPVSGTVVEVN
TALEDDPDFINDEPYGKGWIYKMKPDNIADVDQLLSNTDYEAGL
>Mature_124_residues
MNHPSELKYATTHEWVRIEGDLIVTGISDHAQDALGDLVYVEVPEVGSHMTAGTQAGVVESVKTASDIHAPVSGTVVEVN
TALEDDPDFINDEPYGKGWIYKMKPDNIADVDQLLSNTDYEAGL

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=114, Percent_Identity=42.9824561403509, Blast_Score=109, Evalue=4e-25,
Organism=Homo sapiens, GI89057342, Length=114, Percent_Identity=42.1052631578947, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI1789271, Length=124, Percent_Identity=53.2258064516129, Blast_Score=137, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17507493, Length=112, Percent_Identity=43.75, Blast_Score=112, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17551294, Length=112, Percent_Identity=43.75, Blast_Score=110, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6319272, Length=125, Percent_Identity=43.2, Blast_Score=110, Evalue=5e-26,
Organism=Drosophila melanogaster, GI17865652, Length=120, Percent_Identity=40, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_ACIAD (Q6FB57)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_046528.1
- HSSP:   P16048
- ProteinModelPortal:   Q6FB57
- SMR:   Q6FB57
- STRING:   Q6FB57
- GeneID:   2878490
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD1876
- NMPDR:   fig|62977.3.peg.1790
- eggNOG:   COG0509
- HOGENOM:   HBG693789
- OMA:   TSDHEWL
- PhylomeDB:   Q6FB57
- ProtClustDB:   PRK01202
- BioCyc:   ASP62977:ACIAD1876-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 13441; Mature: 13441

Theoretical pI: Translated: 3.97; Mature: 3.97

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHPSELKYATTHEWVRIEGDLIVTGISDHAQDALGDLVYVEVPEVGSHMTAGTQAGVVE
CCCCCCCEEECCCCEEEEECCEEEECCCHHHHHHHCCEEEEECCCCCCCCCCCCCCCHHH
SVKTASDIHAPVSGTVVEVNTALEDDPDFINDEPYGKGWIYKMKPDNIADVDQLLSNTDY
HHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCCC
EAGL
CCCC
>Mature Secondary Structure
MNHPSELKYATTHEWVRIEGDLIVTGISDHAQDALGDLVYVEVPEVGSHMTAGTQAGVVE
CCCCCCCEEECCCCEEEEECCEEEECCCHHHHHHHCCEEEEECCCCCCCCCCCCCCCHHH
SVKTASDIHAPVSGTVVEVNTALEDDPDFINDEPYGKGWIYKMKPDNIADVDQLLSNTDY
HHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCCC
EAGL
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA