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The map label for this gene is aspA [H]

Identifier: 50084896

GI number: 50084896

Start: 1752232

End: 1753650

Strand: Reverse

Name: aspA [H]

Synonym: ACIAD1744

Alternate gene names: 50084896

Gene position: 1753650-1752232 (Counterclockwise)

Preceding gene: 50084900

Following gene: 50084895

Centisome position: 48.73

GC content: 37.56

Gene sequence:

>1419_bases
ATGAACGCTGAAATTCAAACTCGTACAGAAAAGGATTTACTGGGTCTACGTGAAGTACCAAATCAATTTTACTTCGGTAT
CCAAACTTTACGAGCAATTGAAAACTTCAATCTAAGCTCTAATAAAATCAATCAATATCCAAATTTGATCAAATCATTAG
CGATGGTGAAGCATGCATGTGCAGAAGCAAACCATACTTTGGGTAAATTAAGTGATTTAAAATTTAATGCAATCAAATAT
AGCTGTGATCAGTTAATTCAAGGTTTTTACCACGAACAGTTCCCCATTGACCTCATTCAGGGTGGTGCGGGTACCTCTAC
CAACATGAATGCCAATGAAGTAATTGCAAATATTGCATTAGAGCATTTGGGTCATAAAAAAGGTGAATACAACTTTTTAC
ATCCAAACAATGATGTGAATATGTCTCAATCGACCAATGACGTTTATCCAACTGCAATTCGCTTAGGTTTATTATTCAGC
TTAGATGATTTAAATACGCCTTTTGAAAATTTGATTGCTAGCCTACGCCGTAAGGGTGATGAATTTGCACATATTTTGAA
AATGGGCCGTACTCAGCTTCAAGACGCCGTTCCAATGACTTTGGGTCAAGAATTTAATGCTTTTGCTTCTACACTTGAAA
AAGATTTGATCAAGATTAGAGAAATCGCGCCTCAAACTCTAAATTATATGAATCTTGGCGGTACTGCGATTGGTACAGGA
ATCAATACAGAACAAAAATATACCCAACTTGCCATTGATGCACTTGCGACAATTAGCCAGAAAGATATCAAGTCTGCTCC
CGATTTAATCGAAGCGACTTCAGATATGGGTGATTTTGTATTGTTGTCAGGATTACTTAAACGTACAGCAACTAAACTCT
CAAAAATCGCCAATGATCTACGTTTACTTTCAAGTGGTCCACGTACTGGGATCAATGAAATTAATCTAGAGCCGCGCCAA
CCTGGCAGCTCAATCATGCCAGGTAAAGTAAACCCAGTTATTCCAGAGGCAATGAATCTTGTATGTTTCCAGATTATTGC
GAATGATTTGGCTGTAACTTTATCTGCTGAAGCGGGTCAATTACAATTAAATGCAATGGAACCCCTGATTGCATTCAAAC
TGTTTGAATCAATTGAGTTACTTGGTAATGCGCTCGTCATGTTCCAAACCAAGTGTATCGATACCGTGACTGCAAACGAA
CAACATTGTAAAGCACTTGTCGATAATTCCATTGGAATTGTGACTGCTCTGAATCCTTATCTTGGATATGAAACCACAAC
ACGTATTGCTAAAACTGCAAATGAAAGTGGTTTAAGCGTATTGAGTCTGATCAAATCTGAAAACCTGTTGTCTGATGATC
AACTTTCAGAAATTTTAGCGGTTGACAATATGGTGCAACCGAAAGCTGAAATGGTTTAA

Upstream 100 bases:

>100_bases
CACGGTATGCAAAAAATGCATACTAAAATTAATCTATGCATTAGAAATCATGCAAACCCAATTCTATGATGAATCATCTT
GTCTAGATAGCGATATAAGA

Downstream 100 bases:

>100_bases
GAAATCCCATTTAAAAAGGAACTCCTATGAGTTCCTTTCTTTATTTCTTAGATTGGTTCTAAAATCAACATAAAATTTGA
ATATAAAAAATACAGTTCAA

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MNAEIQTRTEKDLLGLREVPNQFYFGIQTLRAIENFNLSSNKINQYPNLIKSLAMVKHACAEANHTLGKLSDLKFNAIKY
SCDQLIQGFYHEQFPIDLIQGGAGTSTNMNANEVIANIALEHLGHKKGEYNFLHPNNDVNMSQSTNDVYPTAIRLGLLFS
LDDLNTPFENLIASLRRKGDEFAHILKMGRTQLQDAVPMTLGQEFNAFASTLEKDLIKIREIAPQTLNYMNLGGTAIGTG
INTEQKYTQLAIDALATISQKDIKSAPDLIEATSDMGDFVLLSGLLKRTATKLSKIANDLRLLSSGPRTGINEINLEPRQ
PGSSIMPGKVNPVIPEAMNLVCFQIIANDLAVTLSAEAGQLQLNAMEPLIAFKLFESIELLGNALVMFQTKCIDTVTANE
QHCKALVDNSIGIVTALNPYLGYETTTRIAKTANESGLSVLSLIKSENLLSDDQLSEILAVDNMVQPKAEMV

Sequences:

>Translated_472_residues
MNAEIQTRTEKDLLGLREVPNQFYFGIQTLRAIENFNLSSNKINQYPNLIKSLAMVKHACAEANHTLGKLSDLKFNAIKY
SCDQLIQGFYHEQFPIDLIQGGAGTSTNMNANEVIANIALEHLGHKKGEYNFLHPNNDVNMSQSTNDVYPTAIRLGLLFS
LDDLNTPFENLIASLRRKGDEFAHILKMGRTQLQDAVPMTLGQEFNAFASTLEKDLIKIREIAPQTLNYMNLGGTAIGTG
INTEQKYTQLAIDALATISQKDIKSAPDLIEATSDMGDFVLLSGLLKRTATKLSKIANDLRLLSSGPRTGINEINLEPRQ
PGSSIMPGKVNPVIPEAMNLVCFQIIANDLAVTLSAEAGQLQLNAMEPLIAFKLFESIELLGNALVMFQTKCIDTVTANE
QHCKALVDNSIGIVTALNPYLGYETTTRIAKTANESGLSVLSLIKSENLLSDDQLSEILAVDNMVQPKAEMV
>Mature_472_residues
MNAEIQTRTEKDLLGLREVPNQFYFGIQTLRAIENFNLSSNKINQYPNLIKSLAMVKHACAEANHTLGKLSDLKFNAIKY
SCDQLIQGFYHEQFPIDLIQGGAGTSTNMNANEVIANIALEHLGHKKGEYNFLHPNNDVNMSQSTNDVYPTAIRLGLLFS
LDDLNTPFENLIASLRRKGDEFAHILKMGRTQLQDAVPMTLGQEFNAFASTLEKDLIKIREIAPQTLNYMNLGGTAIGTG
INTEQKYTQLAIDALATISQKDIKSAPDLIEATSDMGDFVLLSGLLKRTATKLSKIANDLRLLSSGPRTGINEINLEPRQ
PGSSIMPGKVNPVIPEAMNLVCFQIIANDLAVTLSAEAGQLQLNAMEPLIAFKLFESIELLGNALVMFQTKCIDTVTANE
QHCKALVDNSIGIVTALNPYLGYETTTRIAKTANESGLSVLSLIKSENLLSDDQLSEILAVDNMVQPKAEMV

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=462, Percent_Identity=38.5281385281385, Blast_Score=325, Evalue=5e-89,
Organism=Escherichia coli, GI87082375, Length=461, Percent_Identity=52.4945770065076, Blast_Score=457, Evalue=1e-130,
Organism=Escherichia coli, GI1787896, Length=459, Percent_Identity=39.6514161220044, Blast_Score=343, Evalue=2e-95,
Organism=Caenorhabditis elegans, GI17553882, Length=467, Percent_Identity=36.830835117773, Blast_Score=326, Evalue=1e-89,
Organism=Caenorhabditis elegans, GI32565146, Length=329, Percent_Identity=36.4741641337386, Blast_Score=241, Evalue=6e-64,
Organism=Saccharomyces cerevisiae, GI6324993, Length=462, Percent_Identity=37.012987012987, Blast_Score=320, Evalue=3e-88,
Organism=Drosophila melanogaster, GI24662684, Length=473, Percent_Identity=38.6892177589852, Blast_Score=332, Evalue=2e-91,
Organism=Drosophila melanogaster, GI78710009, Length=470, Percent_Identity=38.5106382978723, Blast_Score=328, Evalue=3e-90,
Organism=Drosophila melanogaster, GI24640179, Length=468, Percent_Identity=37.1794871794872, Blast_Score=321, Evalue=6e-88,
Organism=Drosophila melanogaster, GI24640177, Length=463, Percent_Identity=37.3650107991361, Blast_Score=321, Evalue=7e-88,
Organism=Drosophila melanogaster, GI24583245, Length=462, Percent_Identity=36.5800865800866, Blast_Score=303, Evalue=1e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 51773; Mature: 51773

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAEIQTRTEKDLLGLREVPNQFYFGIQTLRAIENFNLSSNKINQYPNLIKSLAMVKHAC
CCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
AEANHTLGKLSDLKFNAIKYSCDQLIQGFYHEQFPIDLIQGGAGTSTNMNANEVIANIAL
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCHHHHHHHHHH
EHLGHKKGEYNFLHPNNDVNMSQSTNDVYPTAIRLGLLFSLDDLNTPFENLIASLRRKGD
HHHCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHEECCCCCCHHHHHHHHHHCCH
EFAHILKMGRTQLQDAVPMTLGQEFNAFASTLEKDLIKIREIAPQTLNYMNLGGTAIGTG
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCEECCC
INTEQKYTQLAIDALATISQKDIKSAPDLIEATSDMGDFVLLSGLLKRTATKLSKIANDL
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
RLLSSGPRTGINEINLEPRQPGSSIMPGKVNPVIPEAMNLVCFQIIANDLAVTLSAEAGQ
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCE
LQLNAMEPLIAFKLFESIELLGNALVMFQTKCIDTVTANEQHCKALVDNSIGIVTALNPY
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCCC
LGYETTTRIAKTANESGLSVLSLIKSENLLSDDQLSEILAVDNMVQPKAEMV
CCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHCC
>Mature Secondary Structure
MNAEIQTRTEKDLLGLREVPNQFYFGIQTLRAIENFNLSSNKINQYPNLIKSLAMVKHAC
CCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
AEANHTLGKLSDLKFNAIKYSCDQLIQGFYHEQFPIDLIQGGAGTSTNMNANEVIANIAL
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCHHHHHHHHHH
EHLGHKKGEYNFLHPNNDVNMSQSTNDVYPTAIRLGLLFSLDDLNTPFENLIASLRRKGD
HHHCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHEECCCCCCHHHHHHHHHHCCH
EFAHILKMGRTQLQDAVPMTLGQEFNAFASTLEKDLIKIREIAPQTLNYMNLGGTAIGTG
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCEECCC
INTEQKYTQLAIDALATISQKDIKSAPDLIEATSDMGDFVLLSGLLKRTATKLSKIANDL
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
RLLSSGPRTGINEINLEPRQPGSSIMPGKVNPVIPEAMNLVCFQIIANDLAVTLSAEAGQ
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCE
LQLNAMEPLIAFKLFESIELLGNALVMFQTKCIDTVTANEQHCKALVDNSIGIVTALNPY
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCCC
LGYETTTRIAKTANESGLSVLSLIKSENLLSDDQLSEILAVDNMVQPKAEMV
CCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3096982 [H]