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The map label for this gene is gcvA [H]

Identifier: 50084816

GI number: 50084816

Start: 1657741

End: 1658688

Strand: Reverse

Name: gcvA [H]

Synonym: ACIAD1659

Alternate gene names: 50084816

Gene position: 1658688-1657741 (Counterclockwise)

Preceding gene: 50084820

Following gene: 50084814

Centisome position: 46.09

GC content: 38.08

Gene sequence:

>948_bases
GTGCGTCGTATGATTCCGTCTATACAGTCCTTAATTTGTTTTGAATCGGCTGCAAAACATAAAAGCTATACCGATGCAGC
ACAAGAGCTTTCTATCACACAGAGTGCGGTATCCAGACAGATCCAACAATTAGAAGACTTTTTAGATATTGAGCTTTTTA
GTCGTACCAGACATGGCGTTGAATTGACTCAGGCAGGAGAACAATACTATAAAAACATTAAGCCACTTTTACTTGGAATC
GAGCATAGTACGTTAGACTTAATGTCTCATAAAGGCTTAGGGGGTACTCTAAAACTGGGTGTGATACCCACTTTCGCAAC
GCGCTGGTTACTACCACGCTTGTATCGTTTCAATGAAATTTATCCAGAAATCACAATTCACCTCGAAACCAGCACAAAAC
CTTTTTTGTTTAGCGATCATATCTTTGATGCGGCTATTTATGCTGGAACGCCATCTCAAATTGAGAATTGGCCTGGCGCA
AAAATTCAGTTTTTGATGCAAGAAGAAGTCGTACCCGTTTGTTCACCAACTTTAATTAAAAGACATTTTCCAGATATTAC
ACTCAAGCAAAATCAGACTATCAGTTTAAGCCCAGAACAAATCAGTCAATTACCCCTTTTACAGCAAACAACTCGCCCCT
ATATTTGGAAAGAATGGTTCACCTGCATGGGATATGAGTATCCTTATGCAATGGAAGGTCAACGACATGAGCTATTTTCG
ATGCTAGCGGTAGCAGCAACGCATCATCTTGGTGTCGCACTGATTCCACAAATGCTTCTAGAAAAAGAGCTGTTAAATAG
AGAACTGATTGTGATCTCATCTTTAAAGCTAGTAGGTACTCGTTCTTATTATTTTGTTTATTCAGAGCAAAATAATAATA
TACTTATTCAAAAATTCATTGACTGGTTAGAACAAGAAGTAAAGAATTTTCAAAATCAAGTCGTTTGA

Upstream 100 bases:

>100_bases
CGTATCAAAGTGATTATCCTGATGAATCAGATATGAACAATTGCTTGAATTTAGTTTTTAAATTACTCAAAAACATGAGA
AAAAAGCATAAGGAAAAAAT

Downstream 100 bases:

>100_bases
GCAGTAATCATGAGACTGAACTAAAACCAATATATCGAAATAGATTTTGCACCCCTAAATCTGTTTGAATGATTGTTAGC
TGTGGATAAGGCGTTGTAGT

Product: glycine cleavage system transcriptional activator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MRRMIPSIQSLICFESAAKHKSYTDAAQELSITQSAVSRQIQQLEDFLDIELFSRTRHGVELTQAGEQYYKNIKPLLLGI
EHSTLDLMSHKGLGGTLKLGVIPTFATRWLLPRLYRFNEIYPEITIHLETSTKPFLFSDHIFDAAIYAGTPSQIENWPGA
KIQFLMQEEVVPVCSPTLIKRHFPDITLKQNQTISLSPEQISQLPLLQQTTRPYIWKEWFTCMGYEYPYAMEGQRHELFS
MLAVAATHHLGVALIPQMLLEKELLNRELIVISSLKLVGTRSYYFVYSEQNNNILIQKFIDWLEQEVKNFQNQVV

Sequences:

>Translated_315_residues
MRRMIPSIQSLICFESAAKHKSYTDAAQELSITQSAVSRQIQQLEDFLDIELFSRTRHGVELTQAGEQYYKNIKPLLLGI
EHSTLDLMSHKGLGGTLKLGVIPTFATRWLLPRLYRFNEIYPEITIHLETSTKPFLFSDHIFDAAIYAGTPSQIENWPGA
KIQFLMQEEVVPVCSPTLIKRHFPDITLKQNQTISLSPEQISQLPLLQQTTRPYIWKEWFTCMGYEYPYAMEGQRHELFS
MLAVAATHHLGVALIPQMLLEKELLNRELIVISSLKLVGTRSYYFVYSEQNNNILIQKFIDWLEQEVKNFQNQVV
>Mature_315_residues
MRRMIPSIQSLICFESAAKHKSYTDAAQELSITQSAVSRQIQQLEDFLDIELFSRTRHGVELTQAGEQYYKNIKPLLLGI
EHSTLDLMSHKGLGGTLKLGVIPTFATRWLLPRLYRFNEIYPEITIHLETSTKPFLFSDHIFDAAIYAGTPSQIENWPGA
KIQFLMQEEVVPVCSPTLIKRHFPDITLKQNQTISLSPEQISQLPLLQQTTRPYIWKEWFTCMGYEYPYAMEGQRHELFS
MLAVAATHHLGVALIPQMLLEKELLNRELIVISSLKLVGTRSYYFVYSEQNNNILIQKFIDWLEQEVKNFQNQVV

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=305, Percent_Identity=31.4754098360656, Blast_Score=150, Evalue=9e-38,
Organism=Escherichia coli, GI1788706, Length=299, Percent_Identity=31.438127090301, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI1786448, Length=307, Percent_Identity=31.5960912052117, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1786401, Length=333, Percent_Identity=24.3243243243243, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI157672245, Length=122, Percent_Identity=36.8852459016393, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787128, Length=142, Percent_Identity=32.3943661971831, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI145693193, Length=146, Percent_Identity=32.1917808219178, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI145693105, Length=125, Percent_Identity=30.4, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787589, Length=276, Percent_Identity=27.1739130434783, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790208, Length=277, Percent_Identity=28.8808664259928, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787601, Length=123, Percent_Identity=30.0813008130081, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36425; Mature: 36425

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRMIPSIQSLICFESAAKHKSYTDAAQELSITQSAVSRQIQQLEDFLDIELFSRTRHGV
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ELTQAGEQYYKNIKPLLLGIEHSTLDLMSHKGLGGTLKLGVIPTFATRWLLPRLYRFNEI
HHHHHHHHHHHCCCHHEEECCHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHC
YPEITIHLETSTKPFLFSDHIFDAAIYAGTPSQIENWPGAKIQFLMQEEVVPVCSPTLIK
CCEEEEEEECCCCCEEEECHHHHHHEECCCHHHHCCCCCHHEEEEEHHCCCCCCCHHHHH
RHFPDITLKQNQTISLSPEQISQLPLLQQTTRPYIWKEWFTCMGYEYPYAMEGQRHELFS
HCCCCCEECCCCEEEECHHHHHHCCHHHHHCCCHHHHHHHHHHCCCCCCCCCCCHHHHHH
MLAVAATHHLGVALIPQMLLEKELLNRELIVISSLKLVGTRSYYFVYSEQNNNILIQKFI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHEEECCEEEEEEECCCCCEEHHHHH
DWLEQEVKNFQNQVV
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MRRMIPSIQSLICFESAAKHKSYTDAAQELSITQSAVSRQIQQLEDFLDIELFSRTRHGV
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ELTQAGEQYYKNIKPLLLGIEHSTLDLMSHKGLGGTLKLGVIPTFATRWLLPRLYRFNEI
HHHHHHHHHHHCCCHHEEECCHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHC
YPEITIHLETSTKPFLFSDHIFDAAIYAGTPSQIENWPGAKIQFLMQEEVVPVCSPTLIK
CCEEEEEEECCCCCEEEECHHHHHHEECCCHHHHCCCCCHHEEEEEHHCCCCCCCHHHHH
RHFPDITLKQNQTISLSPEQISQLPLLQQTTRPYIWKEWFTCMGYEYPYAMEGQRHELFS
HCCCCCEECCCCEEEECHHHHHHCCHHHHHCCCHHHHHHHHHHCCCCCCCCCCCHHHHHH
MLAVAATHHLGVALIPQMLLEKELLNRELIVISSLKLVGTRSYYFVYSEQNNNILIQKFI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHEEECCEEEEEEECCCCCEEHHHHH
DWLEQEVKNFQNQVV
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]