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The map label for this gene is putP [H]

Identifier: 50084806

GI number: 50084806

Start: 1646466

End: 1647959

Strand: Reverse

Name: putP [H]

Synonym: ACIAD1649

Alternate gene names: 50084806

Gene position: 1647959-1646466 (Counterclockwise)

Preceding gene: 50084807

Following gene: 50084804

Centisome position: 45.79

GC content: 42.5

Gene sequence:

>1494_bases
ATGAATTCCATCAATCCAACCACGATCACGTTTATTTTTTATATCGTGGCAATGGTATGTATTGGTCTCTATGCATATAG
AGCCACTACAGATTTTTCTGATTATATTCTAGGCGGAAGAAGCCTGGGAAGTTTTGTTACGGCATTGTCTGCTGGCGCAT
CGGATATGAGTGGATGGCTACTCATGGGATTGCCAGGTGCAATTTATTTATCTGGTCTTTCTGAAGCATGGATCGCGGTT
GGGCTGATTATTGGCGCCTGGCTTAACTGGTTATTTGTCGCTGGTCGCTTACGTGTACATACTGAGGTTCAGTCTAATGC
ACTTACATTACCAGATTATTTCACCAATCGCTTTAACGACACCAAAAAACTATTGCGTATCTCCTCTGCACTCATCATTT
TGATTTTTTTCTCAATTTACTGTGCATCGGGTATGGTAGCTGGAGCACGACTTTTCGAAACTATTTTTGGCATGGAATAC
CACACAGCCTTATGGATTAGTGCCATTGCAACGATTAGCTATGTTTGTGTTGGTGGCTTTCTGGCAATTAGCTGGACAGA
TACTTTTCAGGCAGGCATGATGTTTTTTGCCCTTATGCTTACCCCTATCTTTGCTTATCTCGCCATTGGTGATGGTCAAC
AACAATTTGCTCAGTTGATTGATGCTGCTCGCCCCCATGCATTTAATATGCTTTCAGGTGTGAGTTTTATCGCGATTATA
TCTGCTATGGCATGGGGTCTGGGCTATTTTGGTCAACCCCATATTTTAGTTCGTTTTATGGCTGCCGACTCAGTAAAATC
AATTCCTGCTGCACGCCGTATTGGTATGTCATGGATGATCTTGTGTCTAGGTGGTGCTGTGGGCGCAGGTTTCTTTGGCA
TTGCATTCTTTCAGGGCCATCCTGAGCTTGCAGGCCATGTGAGTAATAACTCCGAACTTGTGTTTATGGAATTGGCACAA
ATTTTATTTAACCCCTGGGTGGCAGGTATTATCATGGCCGGTATTTTATCGGCAGTCATGAGTACATTAAGCTGTCAGCT
TTTGGTGTGTTCAAGTACACTTACAGAAGATTTTTATAAATCTCTTTTACGTAAAAATGCATCACAAAAAGAGCTCGTAT
GGGTAGGTCGCGTAATGGTGCTTGCGATTTCCGTGTTTGCGATTTTGCTTGCCAGTAATCCTGAAAGTAAAGTTTTAGGT
TTGGTATCATACGCATGGGCAGGTTTCGGTGCAGCTTTTGGCCCATTAATTATCTTATCTTTGTTCTGGAAACGGATGAC
CTTAAACGGTGCTTTGGCAGGTATGGTGGTTGGGGCAGTTGTCGTCATTGGCTGGAAAAATCTGTTTGCTTCAACAGGTA
TTTATGAAATTATTCCAGGGTTTATTTTTGCCGCCATCGCAATTGTGGTCGTCAGTTTAGTTGGCAAACAGCCAAGTAAA
GAAGTGACTGAGCGTTTTGAAGAAGCAGATCGCTTGTATCATAGCTCAAAATAA

Upstream 100 bases:

>100_bases
CATCCGTGTTTATATCACAGCCTTGCTTTAGGTAACCAAATTTTTTTAGCTTGGACTGATTGATTACTCACGTAAATGCA
CATAAGGATAGGTCCCACTC

Downstream 100 bases:

>100_bases
AATTTAGAGAGATCAAGGCATTCATCAGGGGCTTTAAGAAGCCCCTTTTTTTACACTGCGTTTTACCAGTTTAATTTGCG
AAGTCTCTTTTACGACTTGC

Product: SSS family major sodium/proline symporter

Products: NA

Alternate protein names: Proline permease; Propionate transporter [H]

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MNSINPTTITFIFYIVAMVCIGLYAYRATTDFSDYILGGRSLGSFVTALSAGASDMSGWLLMGLPGAIYLSGLSEAWIAV
GLIIGAWLNWLFVAGRLRVHTEVQSNALTLPDYFTNRFNDTKKLLRISSALIILIFFSIYCASGMVAGARLFETIFGMEY
HTALWISAIATISYVCVGGFLAISWTDTFQAGMMFFALMLTPIFAYLAIGDGQQQFAQLIDAARPHAFNMLSGVSFIAII
SAMAWGLGYFGQPHILVRFMAADSVKSIPAARRIGMSWMILCLGGAVGAGFFGIAFFQGHPELAGHVSNNSELVFMELAQ
ILFNPWVAGIIMAGILSAVMSTLSCQLLVCSSTLTEDFYKSLLRKNASQKELVWVGRVMVLAISVFAILLASNPESKVLG
LVSYAWAGFGAAFGPLIILSLFWKRMTLNGALAGMVVGAVVVIGWKNLFASTGIYEIIPGFIFAAIAIVVVSLVGKQPSK
EVTERFEEADRLYHSSK

Sequences:

>Translated_497_residues
MNSINPTTITFIFYIVAMVCIGLYAYRATTDFSDYILGGRSLGSFVTALSAGASDMSGWLLMGLPGAIYLSGLSEAWIAV
GLIIGAWLNWLFVAGRLRVHTEVQSNALTLPDYFTNRFNDTKKLLRISSALIILIFFSIYCASGMVAGARLFETIFGMEY
HTALWISAIATISYVCVGGFLAISWTDTFQAGMMFFALMLTPIFAYLAIGDGQQQFAQLIDAARPHAFNMLSGVSFIAII
SAMAWGLGYFGQPHILVRFMAADSVKSIPAARRIGMSWMILCLGGAVGAGFFGIAFFQGHPELAGHVSNNSELVFMELAQ
ILFNPWVAGIIMAGILSAVMSTLSCQLLVCSSTLTEDFYKSLLRKNASQKELVWVGRVMVLAISVFAILLASNPESKVLG
LVSYAWAGFGAAFGPLIILSLFWKRMTLNGALAGMVVGAVVVIGWKNLFASTGIYEIIPGFIFAAIAIVVVSLVGKQPSK
EVTERFEEADRLYHSSK
>Mature_497_residues
MNSINPTTITFIFYIVAMVCIGLYAYRATTDFSDYILGGRSLGSFVTALSAGASDMSGWLLMGLPGAIYLSGLSEAWIAV
GLIIGAWLNWLFVAGRLRVHTEVQSNALTLPDYFTNRFNDTKKLLRISSALIILIFFSIYCASGMVAGARLFETIFGMEY
HTALWISAIATISYVCVGGFLAISWTDTFQAGMMFFALMLTPIFAYLAIGDGQQQFAQLIDAARPHAFNMLSGVSFIAII
SAMAWGLGYFGQPHILVRFMAADSVKSIPAARRIGMSWMILCLGGAVGAGFFGIAFFQGHPELAGHVSNNSELVFMELAQ
ILFNPWVAGIIMAGILSAVMSTLSCQLLVCSSTLTEDFYKSLLRKNASQKELVWVGRVMVLAISVFAILLASNPESKVLG
LVSYAWAGFGAAFGPLIILSLFWKRMTLNGALAGMVVGAVVVIGWKNLFASTGIYEIIPGFIFAAIAIVVVSLVGKQPSK
EVTERFEEADRLYHSSK

Specific function: Catalyzes the sodium-dependent uptake of extracellular L-proline. This protein is also capable of using lithium as the transport cation. Also catalyzes the uptake of propionate [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI310128183, Length=505, Percent_Identity=46.5346534653465, Blast_Score=445, Evalue=1e-125,
Organism=Homo sapiens, GI110835708, Length=545, Percent_Identity=23.6697247706422, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI14140236, Length=476, Percent_Identity=22.8991596638655, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI206597483, Length=392, Percent_Identity=23.2142857142857, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI206597487, Length=392, Percent_Identity=23.7244897959184, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI4507035, Length=351, Percent_Identity=23.6467236467236, Blast_Score=70, Evalue=6e-12,
Organism=Homo sapiens, GI157671931, Length=424, Percent_Identity=23.3490566037736, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI4507031, Length=468, Percent_Identity=22.008547008547, Blast_Score=67, Evalue=5e-11,
Organism=Homo sapiens, GI11141885, Length=425, Percent_Identity=24.7058823529412, Blast_Score=66, Evalue=8e-11,
Organism=Escherichia coli, GI1787251, Length=492, Percent_Identity=66.6666666666667, Blast_Score=654, Evalue=0.0,
Organism=Escherichia coli, GI87082237, Length=462, Percent_Identity=29.2207792207792, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1790503, Length=447, Percent_Identity=25.2796420581655, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1790113, Length=464, Percent_Identity=24.1379310344828, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI115533094, Length=412, Percent_Identity=24.5145631067961, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI221459588, Length=390, Percent_Identity=26.4102564102564, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI221459584, Length=407, Percent_Identity=23.8329238329238, Blast_Score=86, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24645928, Length=460, Percent_Identity=26.5217391304348, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI221459586, Length=377, Percent_Identity=24.9336870026525, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24640370, Length=355, Percent_Identity=24.5070422535211, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI221459582, Length=407, Percent_Identity=24.5700245700246, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24650192, Length=404, Percent_Identity=23.2673267326733, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24648033, Length=386, Percent_Identity=22.5388601036269, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI21356865, Length=386, Percent_Identity=22.5388601036269, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24651739, Length=486, Percent_Identity=21.3991769547325, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 53836; Mature: 53836

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: PS00456 NA_SOLUT_SYMP_1 ; PS00457 NA_SOLUT_SYMP_2 ; PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSINPTTITFIFYIVAMVCIGLYAYRATTDFSDYILGGRSLGSFVTALSAGASDMSGWL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHCCCCCCCCEE
LMGLPGAIYLSGLSEAWIAVGLIIGAWLNWLFVAGRLRVHTEVQSNALTLPDYFTNRFND
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEECCHHHHHHHHH
TKKLLRISSALIILIFFSIYCASGMVAGARLFETIFGMEYHTALWISAIATISYVCVGGF
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
LAISWTDTFQAGMMFFALMLTPIFAYLAIGDGQQQFAQLIDAARPHAFNMLSGVSFIAII
EEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHH
SAMAWGLGYFGQPHILVRFMAADSVKSIPAARRIGMSWMILCLGGAVGAGFFGIAFFQGH
HHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PELAGHVSNNSELVFMELAQILFNPWVAGIIMAGILSAVMSTLSCQLLVCSSTLTEDFYK
CHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLLRKNASQKELVWVGRVMVLAISVFAILLASNPESKVLGLVSYAWAGFGAAFGPLIILS
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LFWKRMTLNGALAGMVVGAVVVIGWKNLFASTGIYEIIPGFIFAAIAIVVVSLVGKQPSK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCH
EVTERFEEADRLYHSSK
HHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNSINPTTITFIFYIVAMVCIGLYAYRATTDFSDYILGGRSLGSFVTALSAGASDMSGWL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHCCCCCCCCEE
LMGLPGAIYLSGLSEAWIAVGLIIGAWLNWLFVAGRLRVHTEVQSNALTLPDYFTNRFND
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEECCHHHHHHHHH
TKKLLRISSALIILIFFSIYCASGMVAGARLFETIFGMEYHTALWISAIATISYVCVGGF
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
LAISWTDTFQAGMMFFALMLTPIFAYLAIGDGQQQFAQLIDAARPHAFNMLSGVSFIAII
EEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHH
SAMAWGLGYFGQPHILVRFMAADSVKSIPAARRIGMSWMILCLGGAVGAGFFGIAFFQGH
HHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PELAGHVSNNSELVFMELAQILFNPWVAGIIMAGILSAVMSTLSCQLLVCSSTLTEDFYK
CHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLLRKNASQKELVWVGRVMVLAISVFAILLASNPESKVLGLVSYAWAGFGAAFGPLIILS
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LFWKRMTLNGALAGMVVGAVVVIGWKNLFASTGIYEIIPGFIFAAIAIVVVSLVGKQPSK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCH
EVTERFEEADRLYHSSK
HHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3302614; 8905232; 9278503; 1400239; 3325781; 3053687; 3053649; 1567896; 8119910 [H]