Click here to switch to the map view.

The map label for this gene is benB

Identifier: 50084613

GI number: 50084613

Start: 1435605

End: 1436114

Strand: Direct

Name: benB

Synonym: ACIAD1437

Alternate gene names: 50084613

Gene position: 1435605-1436114 (Clockwise)

Preceding gene: 50084612

Following gene: 161349967

Centisome position: 39.89

GC content: 41.18

Gene sequence:

>510_bases
ATGAATGCTACAGCACTTTTAGACACCATCAGCATCGAACAGATTAGCCAGTTTTTGTATAGCGAGGCCCGTTTTTTAGA
TGATGAGCAATGGGATGACTGGCTTGAATGTTATGCACCTCAAGCCTCATTTTGGATGCCCGCGTGGGACGATAACGACC
AGCTCACTGAAAACCCCCAAACTGAGATTTCGCTGATTTATTATCCAGATCGCCAAGGTCTTGAAGATCGAGTATTTCGG
ATTAAAACCGAGCGTTCATCGGCGACCATGCCAGATACGCGTACGGCACACAATATTAGCAATATCGAAGTTGAATCACG
TGATGGCCTTCAAATCACAGTACGTTTTAACTGGAATACGCTCAGTTTCCGCTATAAAAACAGTTACAGCTATTTTGGCA
TGTCACGCTATGTAATCGATTTCTCAGGTGAACAACCAAAAATCTTGAGCAAGTATGTGATGCTTAAGAATGACTATATT
AATCAAGTCATTGATATTTATCATATTTAA

Upstream 100 bases:

>100_bases
ACGAAGGCTTGTATTTGGCACAGCATCAATACTGGCTCAAAAGTATGAAGCAAGCGATTGCTGCGGAAAAAGAATTTGCA
TCGCGTCAGGGAGAGAACGC

Downstream 100 bases:

>100_bases
ATCAGTTATACCTCAGTCAAACGGACACTTGACTGAGGGTTTATGAGTTTATATTTGAATAGGATTCCTGCCATGTCAAA
CCATCAAGTAGCACTTCAAT

Product: benzoate 1,2-dioxygenase beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 169; Mature: 169

Protein sequence:

>169_residues
MNATALLDTISIEQISQFLYSEARFLDDEQWDDWLECYAPQASFWMPAWDDNDQLTENPQTEISLIYYPDRQGLEDRVFR
IKTERSSATMPDTRTAHNISNIEVESRDGLQITVRFNWNTLSFRYKNSYSYFGMSRYVIDFSGEQPKILSKYVMLKNDYI
NQVIDIYHI

Sequences:

>Translated_169_residues
MNATALLDTISIEQISQFLYSEARFLDDEQWDDWLECYAPQASFWMPAWDDNDQLTENPQTEISLIYYPDRQGLEDRVFR
IKTERSSATMPDTRTAHNISNIEVESRDGLQITVRFNWNTLSFRYKNSYSYFGMSRYVIDFSGEQPKILSKYVMLKNDYI
NQVIDIYHI
>Mature_169_residues
MNATALLDTISIEQISQFLYSEARFLDDEQWDDWLECYAPQASFWMPAWDDNDQLTENPQTEISLIYYPDRQGLEDRVFR
IKTERSSATMPDTRTAHNISNIEVESRDGLQITVRFNWNTLSFRYKNSYSYFGMSRYVIDFSGEQPKILSKYVMLKNDYI
NQVIDIYHI

Specific function: Degradation of benzoate to 2-hydro-1,2-dihydroxybenzoate (DHB). The beta subunit may be responsible for the substrate specificity of the enzyme

COG id: COG5517

COG function: function code Q; Small subunit of phenylpropionate dioxygenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial ring-hydroxylating dioxygenase beta subunit family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BENB_ACIAD (P07770)

Other databases:

- EMBL:   AF009224
- EMBL:   CR543861
- PIR:   S23478
- RefSeq:   YP_046123.1
- ProteinModelPortal:   P07770
- STRING:   P07770
- GeneID:   2880891
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD1437
- NMPDR:   fig|62977.3.peg.795
- eggNOG:   COG5517
- HOGENOM:   HBG289528
- OMA:   TSFYTLD
- ProtClustDB:   CLSK707314
- BioCyc:   ASP62977:ACIAD1437-MONOMER
- InterPro:   IPR017641
- InterPro:   IPR000391
- TIGRFAMs:   TIGR03232

Pfam domain/function: PF00866 Ring_hydroxyl_B

EC number: =1.14.12.10

Molecular weight: Translated: 20074; Mature: 20074

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNATALLDTISIEQISQFLYSEARFLDDEQWDDWLECYAPQASFWMPAWDDNDQLTENPQ
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCEECCCCCCCCCCCCCCC
TEISLIYYPDRQGLEDRVFRIKTERSSATMPDTRTAHNISNIEVESRDGLQITVRFNWNT
CEEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEECCCE
LSFRYKNSYSYFGMSRYVIDFSGEQPKILSKYVMLKNDYINQVIDIYHI
EEEEECCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNATALLDTISIEQISQFLYSEARFLDDEQWDDWLECYAPQASFWMPAWDDNDQLTENPQ
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCEECCCCCCCCCCCCCCC
TEISLIYYPDRQGLEDRVFRIKTERSSATMPDTRTAHNISNIEVESRDGLQITVRFNWNT
CEEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEECCCE
LSFRYKNSYSYFGMSRYVIDFSGEQPKILSKYVMLKNDYINQVIDIYHI
EEEEECCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1885518