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The map label for this gene is areA [H]

Identifier: 50084605

GI number: 50084605

Start: 1424279

End: 1425259

Strand: Reverse

Name: areA [H]

Synonym: ACIAD1428

Alternate gene names: 50084605

Gene position: 1425259-1424279 (Counterclockwise)

Preceding gene: 50084606

Following gene: 50084604

Centisome position: 39.61

GC content: 37.1

Gene sequence:

>981_bases
ATGTTACAGAGTTTAGATCCTCAACTGGCAAGTTTTTTTGCAATGCTTGAAAATCAAGGTGCACCTGCATTTGATATTGG
TACTCTAAATGACCCAAATATCAATGAAATGAGAAACTGGTATCTCAATTTTGGTCCTCATGTAGCGGGGCAATTCATTG
AACTTCAAGACGTAGAAGATTTATGGATACAAGTAGACGATGGTAGCTTACGCTTAAGATTGTATAAAACTCAACGCCAG
CATAGTGAATCATGTGAAACACTGATTTACTTCCATGGCGGAGGTTGGTGTATAGGGAGTATCGAAACGCATGATTTACT
TTGTCGGCATTTAGTGAATGATCTAGATGTTAATGTCATTAGTGTCGATTATCGTCTTGCGCCTGAATACCCATACCCTC
AAGCTGCTCATGATGCGATTCATGCCACACAGTGGATTTTTGAACATGCATCTGCTCTTGGCATTCATCCAGACCATTTA
TATGTTGCAGGAGACAGCGCAGGGGGCAATCTGGCAGCAATCACTGCTCTCACTTTTAAAGAACAAACGTATAGAACTTC
TTTAGCAGGGCAAATTTTAATCTACCCATGTATTGATCTTACCGTAATACATGACAACAAATATCCATCCTTTGAAAAAC
AGAAGCTCACTCCTCCTCTATCTGCCGAACAAATGTCAAAGATGATACAAATCTATTTGAAAAATGAAGATTTTGCTTAT
CAATGGCTTGCCTCGCCACTACTCGCTCAAAGTCACCAACATCTTCCATCTACATTAATTATAACAGCCGAATTTGATCC
TTTACATGATCAGGGTTGTGTTTATTTTGAAAAATTAAAAAGCAGTTTAGTAAATACTGAATATCATGAAATAAAAGGTG
TGGTACATGGTTTTTTTGAAATGTATGGCGTACTCAATAAAACAAAAGAGACAATCAATATAATAAGAGCGTGGTTAAAA
AACAATACAAAATCCAAATAA

Upstream 100 bases:

>100_bases
TTTTAAAAATTGGTTAAGCATTGAGAAGTTATGTCTTGAACAATACATAACTTCTCAAATCGTAAATAAATCAAAAAGAT
TTAAAAAGGATTTTTAATAC

Downstream 100 bases:

>100_bases
TCAGTTTTAATTAAAAAGGTTCTTAATAAGAACCTTTTTAATTTACCTGTAAAATTATTATATCAATAATGTATAAATAT
ATACTCTCACATGACTAAAA

Product: esterase for aryl ester catabolic pathway

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MLQSLDPQLASFFAMLENQGAPAFDIGTLNDPNINEMRNWYLNFGPHVAGQFIELQDVEDLWIQVDDGSLRLRLYKTQRQ
HSESCETLIYFHGGGWCIGSIETHDLLCRHLVNDLDVNVISVDYRLAPEYPYPQAAHDAIHATQWIFEHASALGIHPDHL
YVAGDSAGGNLAAITALTFKEQTYRTSLAGQILIYPCIDLTVIHDNKYPSFEKQKLTPPLSAEQMSKMIQIYLKNEDFAY
QWLASPLLAQSHQHLPSTLIITAEFDPLHDQGCVYFEKLKSSLVNTEYHEIKGVVHGFFEMYGVLNKTKETINIIRAWLK
NNTKSK

Sequences:

>Translated_326_residues
MLQSLDPQLASFFAMLENQGAPAFDIGTLNDPNINEMRNWYLNFGPHVAGQFIELQDVEDLWIQVDDGSLRLRLYKTQRQ
HSESCETLIYFHGGGWCIGSIETHDLLCRHLVNDLDVNVISVDYRLAPEYPYPQAAHDAIHATQWIFEHASALGIHPDHL
YVAGDSAGGNLAAITALTFKEQTYRTSLAGQILIYPCIDLTVIHDNKYPSFEKQKLTPPLSAEQMSKMIQIYLKNEDFAY
QWLASPLLAQSHQHLPSTLIITAEFDPLHDQGCVYFEKLKSSLVNTEYHEIKGVVHGFFEMYGVLNKTKETINIIRAWLK
NNTKSK
>Mature_326_residues
MLQSLDPQLASFFAMLENQGAPAFDIGTLNDPNINEMRNWYLNFGPHVAGQFIELQDVEDLWIQVDDGSLRLRLYKTQRQ
HSESCETLIYFHGGGWCIGSIETHDLLCRHLVNDLDVNVISVDYRLAPEYPYPQAAHDAIHATQWIFEHASALGIHPDHL
YVAGDSAGGNLAAITALTFKEQTYRTSLAGQILIYPCIDLTVIHDNKYPSFEKQKLTPPLSAEQMSKMIQIYLKNEDFAY
QWLASPLLAQSHQHLPSTLIITAEFDPLHDQGCVYFEKLKSSLVNTEYHEIKGVVHGFFEMYGVLNKTKETINIIRAWLK
NNTKSK

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=336, Percent_Identity=28.5714285714286, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI206597554, Length=209, Percent_Identity=31.1004784688995, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI157041239, Length=175, Percent_Identity=29.7142857142857, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI68051721, Length=180, Percent_Identity=31.6666666666667, Blast_Score=92, Evalue=9e-19,
Organism=Homo sapiens, GI21328446, Length=187, Percent_Identity=31.5508021390374, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI226423947, Length=189, Percent_Identity=29.6296296296296, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI61966717, Length=180, Percent_Identity=27.2222222222222, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI157041237, Length=142, Percent_Identity=30.2816901408451, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI226423952, Length=133, Percent_Identity=32.3308270676692, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI226423950, Length=133, Percent_Identity=32.3308270676692, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1786682, Length=253, Percent_Identity=28.0632411067194, Blast_Score=88, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI71996133, Length=315, Percent_Identity=24.7619047619048, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17540028, Length=315, Percent_Identity=24.4444444444444, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17567059, Length=267, Percent_Identity=31.4606741573034, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI72001146, Length=156, Percent_Identity=31.4102564102564, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI115533410, Length=177, Percent_Identity=27.1186440677966, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI115533412, Length=177, Percent_Identity=27.1186440677966, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24656084, Length=98, Percent_Identity=39.7959183673469, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24656076, Length=98, Percent_Identity=39.7959183673469, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI20130169, Length=98, Percent_Identity=39.7959183673469, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 37066; Mature: 37066

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS01174 LIPASE_GDXG_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQSLDPQLASFFAMLENQGAPAFDIGTLNDPNINEMRNWYLNFGPHVAGQFIELQDVED
CCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHEECCCCCCCCCEEEEECCCE
LWIQVDDGSLRLRLYKTQRQHSESCETLIYFHGGGWCIGSIETHDLLCRHLVNDLDVNVI
EEEEECCCCEEEEEEECHHHCCCCCCEEEEEECCCEEEECCCHHHHHHHHHHHHCCCEEE
SVDYRLAPEYPYPQAAHDAIHATQWIFEHASALGIHPDHLYVAGDSAGGNLAAITALTFK
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEEEHH
EQTYRTSLAGQILIYPCIDLTVIHDNKYPSFEKQKLTPPLSAEQMSKMIQIYLKNEDFAY
HHHHHHHHCCCEEEEEEEEEEEEECCCCCCCCHHCCCCCCCHHHHHHHHEEEECCCCCHH
QWLASPLLAQSHQHLPSTLIITAEFDPLHDQGCVYFEKLKSSLVNTEYHEIKGVVHGFFE
HHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHH
MYGVLNKTKETINIIRAWLKNNTKSK
HHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLQSLDPQLASFFAMLENQGAPAFDIGTLNDPNINEMRNWYLNFGPHVAGQFIELQDVED
CCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHEECCCCCCCCCEEEEECCCE
LWIQVDDGSLRLRLYKTQRQHSESCETLIYFHGGGWCIGSIETHDLLCRHLVNDLDVNVI
EEEEECCCCEEEEEEECHHHCCCCCCEEEEEECCCEEEECCCHHHHHHHHHHHHCCCEEE
SVDYRLAPEYPYPQAAHDAIHATQWIFEHASALGIHPDHLYVAGDSAGGNLAAITALTFK
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEEEHH
EQTYRTSLAGQILIYPCIDLTVIHDNKYPSFEKQKLTPPLSAEQMSKMIQIYLKNEDFAY
HHHHHHHHCCCEEEEEEEEEEEEECCCCCCCCHHCCCCCCCHHHHHHHHEEEECCCCCHH
QWLASPLLAQSHQHLPSTLIITAEFDPLHDQGCVYFEKLKSSLVNTEYHEIKGVVHGFFE
HHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHH
MYGVLNKTKETINIIRAWLKNNTKSK
HHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]