Click here to switch to the map view.

The map label for this gene is eptA [H]

Identifier: 50084600

GI number: 50084600

Start: 1417886

End: 1419538

Strand: Reverse

Name: eptA [H]

Synonym: ACIAD1423

Alternate gene names: 50084600

Gene position: 1419538-1417886 (Counterclockwise)

Preceding gene: 50084603

Following gene: 50084599

Centisome position: 39.45

GC content: 36.66

Gene sequence:

>1653_bases
ATGTTTAAATTGCTACAATTCAAAAAAGTAAGTTTATCTATAAATACATTTAATGGGCTTCTAGCTTGTTGGTTAGCAAG
TTTTACAAATGCTCGTTTTTATGAACGACTGATTGAATTGAGCTCATTCCAAGGTTTGATGCAATATCTATTTTTGGCTG
GAACTTTTTTGATTCTGGCAGGCACTTATTTTTTCATTCTACAACTGATCAGCTGGCGACTATCAGCCAAACTCATTGCA
GCTATTTTACTTATACTCACAGGCTTTACGTCTTATTTTGTAAATCAGCTTGGTATAGATGTTAATACTGCACAGATGCA
GAATGTCATGCAAACGGATGCGAGTGAAGCACTAGATCTATTATCTTTACCACTTGTTGTATGGTTCGTTTTTACAACGC
TTATACCCCTAATTGTTTTATGTTTTATTGATATTAGAGCTTCCAAAAGCTTAAAAAATCTATTCATATCAAAAAGTGCC
AGTATTATCGTTGCACTTTTTCTTATCGGAATAGGTTTGTTTTCATTTTATGGTCAATACGCTCCAATTTTCCGTGAAAA
TCGTGCCTTAAAAGCACAAATTTCGCCACTCAATATTTTATCGAGTAGCTGGAGTTATACACATAGACAATTTAAAACGC
GCCACTTAGCTTTAATCAAATATGGTGAAGATGCACATCAGATCAATATGACCAGTTTTAATTCACCTCAGGTCATGGTT
TTAGTGATTGGTGAAACTGGCCGTGCTCAAAGCTTTGGTTTAAATGGGTATGCAAGACAAACAACACCTGCACTCAGCAA
ACTTGATATTATCAATTTTAGTCAGGCATCATCTTGTGGTACACAAACTTCGGTATCTGTGCCGTGTATGTTTTCTGGTA
TGCCACGTCAGAATTACGATGAATCTTTAGCAACTCATCGTGAAGGTTTACTTGATATTACACAACGTGCAGGCTATGAC
GTCACTTGGATTGACAATAATTCTGGATGTAAAGGCGCCTGTGATCGTATTAAAAACTACATCTCACCTAAAGATGACCC
GCGTTATCAAGCCCAGTGCAAAGATGGCGAGTGCTACGATGAAGTATTGATTGAAGCCTTAAATGATTATTTAAAAACAC
TTGATCTAAAGAAGCTTAAGAAAAGTCAGTTAATCGTTTTACATCAAGTTGGAAGTCATGGCCCTGCATATTTCAAACGT
TATCCTGCTGCTTTTAAAAAATTTACACCCACTTGTGATACCAACAATATCCAAAACTGCTCACGCGAAAGCTTGATCAA
TAGTTACGACAACACCATTTTATATACCGATCATGTGCTAAGTACATTGATCCATACACTTGATAGTTTGCCTATCTCAA
CAGGTTTGATGTATTTGTCGGATCATGGTGAATCGACTGGTGAAAATGGGTTATATCTCCACGGTACGCCTTATTTTATG
GCACCGAAAGAACAAACCCATATCCCTATGTTATTCTGGTTCTCATCACAATGGCCTAAACAACAACAGATTATGCATTG
TTTAAACCAACAAAAAAATCAGCCAGTCAGTCAGGATAATTTATTTCCAACGGTTTTGAGTTTATTGGACATACAGACCA
AGGCACTTAACCCGCAATTTGATCTGCTGGCTCAATGTAAAGGTAACTTATAA

Upstream 100 bases:

>100_bases
TTTAGATTTAAATTGAATCATTGAAGCGAAAACTAATTTTCTCTTAATTTTCATACTCTTTAATGTAGCTATACCCCTCA
AATAACTGAACGATATTGAG

Downstream 100 bases:

>100_bases
CATGATTAAAATTTTATACATTGAAGATGATGTCATGATTGGCAAGACCACTTATCAGTTATTGCGCCATGAAAAATTTG
AAGTCGACTGGGCTAAAACC

Product: lipid A phosphoethanolamine transferase

Products: NA

Alternate protein names: Polymyxin resistance protein pmrC [H]

Number of amino acids: Translated: 550; Mature: 550

Protein sequence:

>550_residues
MFKLLQFKKVSLSINTFNGLLACWLASFTNARFYERLIELSSFQGLMQYLFLAGTFLILAGTYFFILQLISWRLSAKLIA
AILLILTGFTSYFVNQLGIDVNTAQMQNVMQTDASEALDLLSLPLVVWFVFTTLIPLIVLCFIDIRASKSLKNLFISKSA
SIIVALFLIGIGLFSFYGQYAPIFRENRALKAQISPLNILSSSWSYTHRQFKTRHLALIKYGEDAHQINMTSFNSPQVMV
LVIGETGRAQSFGLNGYARQTTPALSKLDIINFSQASSCGTQTSVSVPCMFSGMPRQNYDESLATHREGLLDITQRAGYD
VTWIDNNSGCKGACDRIKNYISPKDDPRYQAQCKDGECYDEVLIEALNDYLKTLDLKKLKKSQLIVLHQVGSHGPAYFKR
YPAAFKKFTPTCDTNNIQNCSRESLINSYDNTILYTDHVLSTLIHTLDSLPISTGLMYLSDHGESTGENGLYLHGTPYFM
APKEQTHIPMLFWFSSQWPKQQQIMHCLNQQKNQPVSQDNLFPTVLSLLDIQTKALNPQFDLLAQCKGNL

Sequences:

>Translated_550_residues
MFKLLQFKKVSLSINTFNGLLACWLASFTNARFYERLIELSSFQGLMQYLFLAGTFLILAGTYFFILQLISWRLSAKLIA
AILLILTGFTSYFVNQLGIDVNTAQMQNVMQTDASEALDLLSLPLVVWFVFTTLIPLIVLCFIDIRASKSLKNLFISKSA
SIIVALFLIGIGLFSFYGQYAPIFRENRALKAQISPLNILSSSWSYTHRQFKTRHLALIKYGEDAHQINMTSFNSPQVMV
LVIGETGRAQSFGLNGYARQTTPALSKLDIINFSQASSCGTQTSVSVPCMFSGMPRQNYDESLATHREGLLDITQRAGYD
VTWIDNNSGCKGACDRIKNYISPKDDPRYQAQCKDGECYDEVLIEALNDYLKTLDLKKLKKSQLIVLHQVGSHGPAYFKR
YPAAFKKFTPTCDTNNIQNCSRESLINSYDNTILYTDHVLSTLIHTLDSLPISTGLMYLSDHGESTGENGLYLHGTPYFM
APKEQTHIPMLFWFSSQWPKQQQIMHCLNQQKNQPVSQDNLFPTVLSLLDIQTKALNPQFDLLAQCKGNL
>Mature_550_residues
MFKLLQFKKVSLSINTFNGLLACWLASFTNARFYERLIELSSFQGLMQYLFLAGTFLILAGTYFFILQLISWRLSAKLIA
AILLILTGFTSYFVNQLGIDVNTAQMQNVMQTDASEALDLLSLPLVVWFVFTTLIPLIVLCFIDIRASKSLKNLFISKSA
SIIVALFLIGIGLFSFYGQYAPIFRENRALKAQISPLNILSSSWSYTHRQFKTRHLALIKYGEDAHQINMTSFNSPQVMV
LVIGETGRAQSFGLNGYARQTTPALSKLDIINFSQASSCGTQTSVSVPCMFSGMPRQNYDESLATHREGLLDITQRAGYD
VTWIDNNSGCKGACDRIKNYISPKDDPRYQAQCKDGECYDEVLIEALNDYLKTLDLKKLKKSQLIVLHQVGSHGPAYFKR
YPAAFKKFTPTCDTNNIQNCSRESLINSYDNTILYTDHVLSTLIHTLDSLPISTGLMYLSDHGESTGENGLYLHGTPYFM
APKEQTHIPMLFWFSSQWPKQQQIMHCLNQQKNQPVSQDNLFPTVLSLLDIQTKALNPQFDLLAQCKGNL

Specific function: Catalyzes the addition of a phosphoethanolamine moiety to the lipid A. The phosphoethanolamine modification is required for resistance to polymyxin [H]

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptA subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082372, Length=525, Percent_Identity=39.6190476190476, Blast_Score=402, Evalue=1e-113,
Organism=Escherichia coli, GI87082286, Length=564, Percent_Identity=24.468085106383, Blast_Score=142, Evalue=7e-35,
Organism=Escherichia coli, GI87082223, Length=478, Percent_Identity=25.5230125523013, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1790392, Length=542, Percent_Identity=23.6162361623616, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1787035, Length=474, Percent_Identity=23.4177215189873, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 62017; Mature: 62017

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00041 HTH_ARAC_FAMILY_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKLLQFKKVSLSINTFNGLLACWLASFTNARFYERLIELSSFQGLMQYLFLAGTFLILA
CCCCCEEEEEEEEEEHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GTYFFILQLISWRLSAKLIAAILLILTGFTSYFVNQLGIDVNTAQMQNVMQTDASEALDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LSLPLVVWFVFTTLIPLIVLCFIDIRASKSLKNLFISKSASIIVALFLIGIGLFSFYGQY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCC
APIFRENRALKAQISPLNILSSSWSYTHRQFKTRHLALIKYGEDAHQINMTSFNSPQVMV
CCHHHCCCCEEEECCCHHHHHCCCHHHHHHHHHHEEEEEEECCCCCEEEEECCCCCCEEE
LVIGETGRAQSFGLNGYARQTTPALSKLDIINFSQASSCGTQTSVSVPCMFSGMPRQNYD
EEECCCCCCCCCCCCCCCCCCCCHHHHCCEECCCCCCCCCCCCCCCCCEEECCCCCCCHH
ESLATHREGLLDITQRAGYDVTWIDNNSGCKGACDRIKNYISPKDDPRYQAQCKDGECYD
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHH
EVLIEALNDYLKTLDLKKLKKSQLIVLHQVGSHGPAYFKRYPAAFKKFTPTCDTNNIQNC
HHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
SRESLINSYDNTILYTDHVLSTLIHTLDSLPISTGLMYLSDHGESTGENGLYLHGTPYFM
CHHHHHHHCCCEEEEHHHHHHHHHHHHHCCCHHHCEEEEECCCCCCCCCCEEEECCCEEE
APKEQTHIPMLFWFSSQWPKQQQIMHCLNQQKNQPVSQDNLFPTVLSLLDIQTKALNPQF
CCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHH
DLLAQCKGNL
HHHHHCCCCC
>Mature Secondary Structure
MFKLLQFKKVSLSINTFNGLLACWLASFTNARFYERLIELSSFQGLMQYLFLAGTFLILA
CCCCCEEEEEEEEEEHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GTYFFILQLISWRLSAKLIAAILLILTGFTSYFVNQLGIDVNTAQMQNVMQTDASEALDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LSLPLVVWFVFTTLIPLIVLCFIDIRASKSLKNLFISKSASIIVALFLIGIGLFSFYGQY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCC
APIFRENRALKAQISPLNILSSSWSYTHRQFKTRHLALIKYGEDAHQINMTSFNSPQVMV
CCHHHCCCCEEEECCCHHHHHCCCHHHHHHHHHHEEEEEEECCCCCEEEEECCCCCCEEE
LVIGETGRAQSFGLNGYARQTTPALSKLDIINFSQASSCGTQTSVSVPCMFSGMPRQNYD
EEECCCCCCCCCCCCCCCCCCCCHHHHCCEECCCCCCCCCCCCCCCCCEEECCCCCCCHH
ESLATHREGLLDITQRAGYDVTWIDNNSGCKGACDRIKNYISPKDDPRYQAQCKDGECYD
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHH
EVLIEALNDYLKTLDLKKLKKSQLIVLHQVGSHGPAYFKRYPAAFKKFTPTCDTNNIQNC
HHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
SRESLINSYDNTILYTDHVLSTLIHTLDSLPISTGLMYLSDHGESTGENGLYLHGTPYFM
CHHHHHHHCCCEEEEHHHHHHHHHHHHHCCCHHHCEEEEECCCCCCCCCCEEEECCCEEE
APKEQTHIPMLFWFSSQWPKQQQIMHCLNQQKNQPVSQDNLFPTVLSLLDIQTKALNPQF
CCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHH
DLLAQCKGNL
HHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8391535; 11677609 [H]