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The map label for this gene is alkR

Identifier: 50084587

GI number: 50084587

Start: 1403617

End: 1404537

Strand: Reverse

Name: alkR

Synonym: ACIAD1410

Alternate gene names: 50084587

Gene position: 1404537-1403617 (Counterclockwise)

Preceding gene: 50084589

Following gene: 50084583

Centisome position: 39.03

GC content: 39.96

Gene sequence:

>921_bases
ATGGATGCACTTAGTAAAATTTTTGATGATATTCATTTAAATAAATCTGAATATCTCTATGTAAAAACTCAAGGAGAATG
GGCATTTCATATGCTTGCCCAAGATGCTTTGATTGCTCACATTATTTTAATGGGTTCTGCTCATTTCACATTACAGGATG
GTACGACACAAACCGCTTATTCGGGAGATATTGTGCTTATTCCTTCTGGGCAAGCTCATTTTGTCTCAAATGATGCTCAG
AACAAACTAATTGATTGTTCCAACATTCAGTCTTTTTTTGATGGACACCGCAATGATGCCATTGAGTTAGGCACAACATC
TTCGGATCATGGTTTAATTTTTACTGTGCGGAGCCATATCGATATGCATATGGGTAGCCCTTTACTCAATGCTTTACCAT
CACTGATACATATTCATCACGCAATGAGCTCAACTGGCCCAGAGTGGTTACGCGTCGGTTTATATTTTGTGGCACTTGAA
ACCCAGCGTATTCAACCAGGACGAGATAAAATATTTGACCACTTGATGAGCATCCTATTTATAGAATGTGTCCGCGATCA
TATTGCCCAGCTTGATGACCCCAAAAGCTGGTTAACTGCACTCATGCATCCCGAATTATCTAATGCGCTCGCAGCTATTC
ATGCTTACCCCGAAAAACCTTGGACTGTAGAGAGTTTGGCAGATCAATGTTGCATGTCACGGTCTAAATTTGCCACACTT
TTTCAAAGTATTGTTCATGAGACCCCTCTCGCTTATCTTCAACAACATCGCCTTCGTCTGGCCGTACAGTTATTAAAAAC
CAGTCAGTTAAATATTCAGCAAATTGCCAATAAAGTCGGATACTCCTCAGAAACAGCTTTTAGTCAGGCATTTAAACGTC
AATTTGAACAATCTCCTAAACACTATCGCCAGCAATCATGA

Upstream 100 bases:

>100_bases
AAGTCTTTGGCATTTTCAACCATGTCAATGTCCTAAAATCACATGTATAAGCTACAATCTCATTCTATACTCTTGCTCAA
ATAAGAATTTTTAACGCGCT

Downstream 100 bases:

>100_bases
TAAAAAAAGCGCCGAATATGGCGCTTTTTTATTTCAAAGTCAAAGATTATTTAATTTTGGCGTGTGATTTTAAATACTGA
GTATAATCATCAAGTTCCTG

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MDALSKIFDDIHLNKSEYLYVKTQGEWAFHMLAQDALIAHIILMGSAHFTLQDGTTQTAYSGDIVLIPSGQAHFVSNDAQ
NKLIDCSNIQSFFDGHRNDAIELGTTSSDHGLIFTVRSHIDMHMGSPLLNALPSLIHIHHAMSSTGPEWLRVGLYFVALE
TQRIQPGRDKIFDHLMSILFIECVRDHIAQLDDPKSWLTALMHPELSNALAAIHAYPEKPWTVESLADQCCMSRSKFATL
FQSIVHETPLAYLQQHRLRLAVQLLKTSQLNIQQIANKVGYSSETAFSQAFKRQFEQSPKHYRQQS

Sequences:

>Translated_306_residues
MDALSKIFDDIHLNKSEYLYVKTQGEWAFHMLAQDALIAHIILMGSAHFTLQDGTTQTAYSGDIVLIPSGQAHFVSNDAQ
NKLIDCSNIQSFFDGHRNDAIELGTTSSDHGLIFTVRSHIDMHMGSPLLNALPSLIHIHHAMSSTGPEWLRVGLYFVALE
TQRIQPGRDKIFDHLMSILFIECVRDHIAQLDDPKSWLTALMHPELSNALAAIHAYPEKPWTVESLADQCCMSRSKFATL
FQSIVHETPLAYLQQHRLRLAVQLLKTSQLNIQQIANKVGYSSETAFSQAFKRQFEQSPKHYRQQS
>Mature_306_residues
MDALSKIFDDIHLNKSEYLYVKTQGEWAFHMLAQDALIAHIILMGSAHFTLQDGTTQTAYSGDIVLIPSGQAHFVSNDAQ
NKLIDCSNIQSFFDGHRNDAIELGTTSSDHGLIFTVRSHIDMHMGSPLLNALPSLIHIHHAMSSTGPEWLRVGLYFVALE
TQRIQPGRDKIFDHLMSILFIECVRDHIAQLDDPKSWLTALMHPELSNALAAIHAYPEKPWTVESLADQCCMSRSKFATL
FQSIVHETPLAYLQQHRLRLAVQLLKTSQLNIQQIANKVGYSSETAFSQAFKRQFEQSPKHYRQQS

Specific function: This protein activates the expression of the alkane 1- monooxygenase AlkM

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1786496, Length=311, Percent_Identity=29.903536977492, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1790497, Length=98, Percent_Identity=31.6326530612245, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): ALKR_ACIAD (O31249)

Other databases:

- EMBL:   AJ002316
- EMBL:   CR543861
- RefSeq:   YP_046097.1
- ProteinModelPortal:   O31249
- SMR:   O31249
- STRING:   O31249
- GeneID:   2880707
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD1410
- NMPDR:   fig|62977.3.peg.711
- eggNOG:   COG2207
- HOGENOM:   HBG289368
- OMA:   FELGHPW
- PhylomeDB:   O31249
- ProtClustDB:   CLSK707308
- BioCyc:   ASP62977:ACIAD1410-MONOMER
- GO:   GO:0005622
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060
- Gene3D:   G3DSA:1.10.10.60
- PRINTS:   PR00032
- SMART:   SM00342

Pfam domain/function: PF00165 HTH_AraC; SSF46689 Homeodomain_like

EC number: NA

Molecular weight: Translated: 34581; Mature: 34581

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00041 HTH_ARAC_FAMILY_1; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDALSKIFDDIHLNKSEYLYVKTQGEWAFHMLAQDALIAHIILMGSAHFTLQDGTTQTAY
CCHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEE
SGDIVLIPSGQAHFVSNDAQNKLIDCSNIQSFFDGHRNDAIELGTTSSDHGLIFTVRSHI
CCCEEEEECCCCEEECCCCHHCCCCHHHHHHHHCCCCCCCEEECCCCCCCCEEEEEHHHH
DMHMGSPLLNALPSLIHIHHAMSSTGPEWLRVGLYFVALETQRIQPGRDKIFDHLMSILF
HHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEEEHHCCCCCHHHHHHHHHHHHH
IECVRDHIAQLDDPKSWLTALMHPELSNALAAIHAYPEKPWTVESLADQCCMSRSKFATL
HHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
FQSIVHETPLAYLQQHRLRLAVQLLKTSQLNIQQIANKVGYSSETAFSQAFKRQFEQSPK
HHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCHH
HYRQQS
HHCCCC
>Mature Secondary Structure
MDALSKIFDDIHLNKSEYLYVKTQGEWAFHMLAQDALIAHIILMGSAHFTLQDGTTQTAY
CCHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEE
SGDIVLIPSGQAHFVSNDAQNKLIDCSNIQSFFDGHRNDAIELGTTSSDHGLIFTVRSHI
CCCEEEEECCCCEEECCCCHHCCCCHHHHHHHHCCCCCCCEEECCCCCCCCEEEEEHHHH
DMHMGSPLLNALPSLIHIHHAMSSTGPEWLRVGLYFVALETQRIQPGRDKIFDHLMSILF
HHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEEEHHCCCCCHHHHHHHHHHHHH
IECVRDHIAQLDDPKSWLTALMHPELSNALAAIHAYPEKPWTVESLADQCCMSRSKFATL
HHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
FQSIVHETPLAYLQQHRLRLAVQLLKTSQLNIQQIANKVGYSSETAFSQAFKRQFEQSPK
HHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCHH
HYRQQS
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9546151