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The map label for this gene is tkrA [H]

Identifier: 50084508

GI number: 50084508

Start: 1324892

End: 1325857

Strand: Direct

Name: tkrA [H]

Synonym: ACIAD1327

Alternate gene names: 50084508

Gene position: 1324892-1325857 (Clockwise)

Preceding gene: 50084507

Following gene: 50084509

Centisome position: 36.82

GC content: 39.75

Gene sequence:

>966_bases
ATGAAAAAAAAGGTTGTTGTATTTAGTCAGATAGATCCGCGTATTTTAGAGCAATTAAAGCAGCAATATCAGGTTGTTGT
TCTGGATCCTAAAAAAGGTGATATCAATGAACAGATCTGTCAAGAAGTAGTTGATGCAGATGGCATGATTGGTGCAGGGC
GTTTACTCAATGAAAACAACCTTGCTCCTGCACAGCATCTTAAAATTGTTTCTACAGTCAGCGTTGGCTATGACAACTAC
GATGTGCAGTATCTTAATCAGAAAAAAATCTGGTTGGCACATACTCCTCATGTATTAACTGAGACTACAGCAGACCTTGC
TTTTACTTTGCTCGTGAGTGCTGCAAGAAAAGTTCCGCAACTTGATGCATGGACCAAAGCTGGCGAATGGAAAAGAACGG
TAGGTGCAGCTCAATTTGGGCAGGATATTTTTGGTAAAACACTTGGTATCATTGGTCTGGGTAATATTGGTGCGGCAATT
GCTCGACGTGGTTTGTATGGCTTCAATATGAATATTTTATATCATAACCGTCATGAAAAACTGGTGCTGGCTCAGCCTTT
AAACGCTGAATACTGTTCAATGCAAGATTTACTTCAACGTTCAGATTTTATCGTGGTTGCGGTAGATTTAAATACTGAAT
CTCAAGCACTGATTGGTACTCAAGAATTTGAGCAGATGCAGTCACATGCTGTATTTGTAAATATTTCAAGAGGCTCTGTG
GTTGATGAAAGCGCATTGATTGAGGCGCTTAAGCAAAAGCAAATTTTTGCTGCTGGTTTGGATGTATATCAAAAAGAGCC
GTTGCAAGCATCTGAATTGTTTGATCTGGATAATGTGGTGACATTGCCACATGTAGGCTCCGCTACAGCAGAGACACGAT
TGAAGATGTCACAACTGGCCTATAAAAATCTGGTTGATGCCTTAGAAGGTAAAACGCCTCGTTATTTAGTTAATCCAGAT
TTTTAA

Upstream 100 bases:

>100_bases
GCGCAATTGATGCCGCTTGCAAGCTTGGCAGAAATGCTCGAGCAGCCAAGACTGAAAAAACGGTTATGGACATTGATGCA
GTCCGCTTAAAGGGAAGATG

Downstream 100 bases:

>100_bases
TCATCTTTAAGCGCTAAGGTTTTTATTTTTAGAAAGTATTGGGATACTTTAAAATTTCATAACACTTTATTTCGAATTTG
AAAATTCTGGAATGTTATAG

Product: 2-keto-D-gluconate reductase (2-ketoaldonate reductase)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MKKKVVVFSQIDPRILEQLKQQYQVVVLDPKKGDINEQICQEVVDADGMIGAGRLLNENNLAPAQHLKIVSTVSVGYDNY
DVQYLNQKKIWLAHTPHVLTETTADLAFTLLVSAARKVPQLDAWTKAGEWKRTVGAAQFGQDIFGKTLGIIGLGNIGAAI
ARRGLYGFNMNILYHNRHEKLVLAQPLNAEYCSMQDLLQRSDFIVVAVDLNTESQALIGTQEFEQMQSHAVFVNISRGSV
VDESALIEALKQKQIFAAGLDVYQKEPLQASELFDLDNVVTLPHVGSATAETRLKMSQLAYKNLVDALEGKTPRYLVNPD
F

Sequences:

>Translated_321_residues
MKKKVVVFSQIDPRILEQLKQQYQVVVLDPKKGDINEQICQEVVDADGMIGAGRLLNENNLAPAQHLKIVSTVSVGYDNY
DVQYLNQKKIWLAHTPHVLTETTADLAFTLLVSAARKVPQLDAWTKAGEWKRTVGAAQFGQDIFGKTLGIIGLGNIGAAI
ARRGLYGFNMNILYHNRHEKLVLAQPLNAEYCSMQDLLQRSDFIVVAVDLNTESQALIGTQEFEQMQSHAVFVNISRGSV
VDESALIEALKQKQIFAAGLDVYQKEPLQASELFDLDNVVTLPHVGSATAETRLKMSQLAYKNLVDALEGKTPRYLVNPD
F
>Mature_321_residues
MKKKVVVFSQIDPRILEQLKQQYQVVVLDPKKGDINEQICQEVVDADGMIGAGRLLNENNLAPAQHLKIVSTVSVGYDNY
DVQYLNQKKIWLAHTPHVLTETTADLAFTLLVSAARKVPQLDAWTKAGEWKRTVGAAQFGQDIFGKTLGIIGLGNIGAAI
ARRGLYGFNMNILYHNRHEKLVLAQPLNAEYCSMQDLLQRSDFIVVAVDLNTESQALIGTQEFEQMQSHAVFVNISRGSV
VDESALIEALKQKQIFAAGLDVYQKEPLQASELFDLDNVVTLPHVGSATAETRLKMSQLAYKNLVDALEGKTPRYLVNPD
F

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrB subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=252, Percent_Identity=42.4603174603175, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI23308577, Length=317, Percent_Identity=28.7066246056782, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI61743967, Length=296, Percent_Identity=30.4054054054054, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI4557497, Length=296, Percent_Identity=30.4054054054054, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI145580575, Length=302, Percent_Identity=29.1390728476821, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI145580578, Length=302, Percent_Identity=29.1390728476821, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI4557499, Length=302, Percent_Identity=29.1390728476821, Blast_Score=123, Evalue=3e-28,
Organism=Escherichia coli, GI87082289, Length=323, Percent_Identity=43.9628482972136, Blast_Score=276, Evalue=1e-75,
Organism=Escherichia coli, GI1789279, Length=299, Percent_Identity=28.0936454849498, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787645, Length=345, Percent_Identity=27.2463768115942, Blast_Score=99, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17532191, Length=304, Percent_Identity=29.6052631578947, Blast_Score=140, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI25147481, Length=297, Percent_Identity=25.5892255892256, Blast_Score=99, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6324055, Length=287, Percent_Identity=35.191637630662, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6320925, Length=271, Percent_Identity=27.3062730627306, Blast_Score=115, Evalue=9e-27,
Organism=Saccharomyces cerevisiae, GI6322116, Length=251, Percent_Identity=28.6852589641434, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6321253, Length=295, Percent_Identity=25.7627118644068, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6325144, Length=196, Percent_Identity=30.1020408163265, Blast_Score=82, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6324964, Length=290, Percent_Identity=23.7931034482759, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI28574286, Length=323, Percent_Identity=37.1517027863777, Blast_Score=185, Evalue=4e-47,
Organism=Drosophila melanogaster, GI28574284, Length=288, Percent_Identity=39.2361111111111, Blast_Score=181, Evalue=7e-46,
Organism=Drosophila melanogaster, GI45551003, Length=288, Percent_Identity=39.2361111111111, Blast_Score=181, Evalue=8e-46,
Organism=Drosophila melanogaster, GI24585514, Length=288, Percent_Identity=39.2361111111111, Blast_Score=181, Evalue=8e-46,
Organism=Drosophila melanogaster, GI28574282, Length=288, Percent_Identity=39.2361111111111, Blast_Score=181, Evalue=8e-46,
Organism=Drosophila melanogaster, GI45552429, Length=288, Percent_Identity=39.2361111111111, Blast_Score=180, Evalue=9e-46,
Organism=Drosophila melanogaster, GI28571528, Length=250, Percent_Identity=39.2, Blast_Score=168, Evalue=5e-42,
Organism=Drosophila melanogaster, GI24585516, Length=278, Percent_Identity=37.0503597122302, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI19921140, Length=318, Percent_Identity=27.6729559748428, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24646446, Length=310, Percent_Identity=29.0322580645161, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24646448, Length=310, Percent_Identity=29.0322580645161, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24646452, Length=310, Percent_Identity=29.0322580645161, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24646450, Length=310, Percent_Identity=29.0322580645161, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI62472511, Length=294, Percent_Identity=29.2517006802721, Blast_Score=115, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 35651; Mature: 35651

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKVVVFSQIDPRILEQLKQQYQVVVLDPKKGDINEQICQEVVDADGMIGAGRLLNENN
CCCEEEEEECCCHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHCCCCCCCCCEEECCCC
LAPAQHLKIVSTVSVGYDNYDVQYLNQKKIWLAHTPHVLTETTADLAFTLLVSAARKVPQ
CCHHHHEEEEEEEEECCCCCCEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCC
LDAWTKAGEWKRTVGAAQFGQDIFGKTLGIIGLGNIGAAIARRGLYGFNMNILYHNRHEK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCEEEECHHHHHHHHCCCCEEEEEEEEECCCCE
LVLAQPLNAEYCSMQDLLQRSDFIVVAVDLNTESQALIGTQEFEQMQSHAVFVNISRGSV
EEEECCCCCHHCCHHHHHCCCCEEEEEEECCCCCCCEECHHHHHHHHCCEEEEEECCCCC
VDESALIEALKQKQIFAAGLDVYQKEPLQASELFDLDNVVTLPHVGSATAETRLKMSQLA
CCHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHCCCCCEEECCCCCCCHHHHHHHHHHHH
YKNLVDALEGKTPRYLVNPDF
HHHHHHHHCCCCCEEEECCCC
>Mature Secondary Structure
MKKKVVVFSQIDPRILEQLKQQYQVVVLDPKKGDINEQICQEVVDADGMIGAGRLLNENN
CCCEEEEEECCCHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHCCCCCCCCCEEECCCC
LAPAQHLKIVSTVSVGYDNYDVQYLNQKKIWLAHTPHVLTETTADLAFTLLVSAARKVPQ
CCHHHHEEEEEEEEECCCCCCEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCC
LDAWTKAGEWKRTVGAAQFGQDIFGKTLGIIGLGNIGAAIARRGLYGFNMNILYHNRHEK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCEEEECHHHHHHHHCCCCEEEEEEEEECCCCE
LVLAQPLNAEYCSMQDLLQRSDFIVVAVDLNTESQALIGTQEFEQMQSHAVFVNISRGSV
EEEECCCCCHHCCHHHHHCCCCEEEEEEECCCCCCCEECHHHHHHHHCCEEEEEECCCCC
VDESALIEALKQKQIFAAGLDVYQKEPLQASELFDLDNVVTLPHVGSATAETRLKMSQLA
CCHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHCCCCCEEECCCCCCCHHHHHHHHHHHH
YKNLVDALEGKTPRYLVNPDF
HHHHHHHHCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA