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The map label for this gene is artJ [H]

Identifier: 50084480

GI number: 50084480

Start: 1298781

End: 1299584

Strand: Direct

Name: artJ [H]

Synonym: ACIAD1297

Alternate gene names: 50084480

Gene position: 1298781-1299584 (Clockwise)

Preceding gene: 50084479

Following gene: 50084481

Centisome position: 36.09

GC content: 41.79

Gene sequence:

>804_bases
ATGAAACAGACCTTTAAAAATTTTTCATGTCGGATGGCTTTATCTGTCGCCGCAGTCACATGCTTTTCATCAATGAGCCA
TGCTGCACCATTACTCACCAATGGCGTGTTTAAAGTCGGTTTAGAGGCAACTTATCCACCGTTTGAATCTTATGACAGCA
ATAAAAATATCGTTGGTCTTGATCCAGATTTTGCTGCGTTGATTGCCAAAGATTTAAATGCTAAACCTCAACTGATTGAT
ACCAAGTTTACCAGCCTGATTTTGGGTCTGGGTAAAAAGTATGATGCGGTTATTTCAGGGTTATATGTCACTCCAGAGCG
TCTAAAACAAGCAGATGCCATTCCTTATGCCATGACGGGTGCTTCAATTATTGCGCTTAAAAATAGCAACATTTTACCAA
AGACTGAAAATGATTTGTGCGGTGTAAAAGTAGGTTTGCAGCAAGGAACTGCATGGGTGGCCAGTCTTAAGAAACATTCA
GAGGCTTATTGTATTAAAAATGGAAAACCCGCCATTCAGGTGATGGAGTTTCCAACAGCTCCTGAAGTCTCTCAGGCATT
GATGTCAAGAAACGTAAGTGCACAACTGGAAATTGCGCCAGCGGCAAAAATTCTCGTTGAGAAAAGTCGTGGTCGTCTTG
TAGTGAGTTCTTCACGTCTGGTCTATCCATTGCCACTCGGGATCTATGTCACCAAAGGAAATACCGCACTTGTCTCAGCC
ATTAAATCATCGCTCGCGAAAGCCAAGGCCAATGGTCAATATAGCGCGCTGATCAAAAAATATAATTTAGAGCCTGTTCA
TTAA

Upstream 100 bases:

>100_bases
GAGTTGCAATTATCAGAAAGGGCGAGAGCAACCCATACTTTTGGTCACCTGAAACAACAAGCAAAGCAAAAAACCACTAG
CAATTTGTGAGGATTGATTT

Downstream 100 bases:

>100_bases
TTGTTTGAGATTGGGAGTCAGTATGGAATTCGATTGGGAATATTTCTTCTCTTTATTTGGCTTTGCAGAGTTTTGGAAGG
CCTGCGTAACTGTCATTGTA

Product: amino acid ABC transporter periplasmic substrate-binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MKQTFKNFSCRMALSVAAVTCFSSMSHAAPLLTNGVFKVGLEATYPPFESYDSNKNIVGLDPDFAALIAKDLNAKPQLID
TKFTSLILGLGKKYDAVISGLYVTPERLKQADAIPYAMTGASIIALKNSNILPKTENDLCGVKVGLQQGTAWVASLKKHS
EAYCIKNGKPAIQVMEFPTAPEVSQALMSRNVSAQLEIAPAAKILVEKSRGRLVVSSSRLVYPLPLGIYVTKGNTALVSA
IKSSLAKAKANGQYSALIKKYNLEPVH

Sequences:

>Translated_267_residues
MKQTFKNFSCRMALSVAAVTCFSSMSHAAPLLTNGVFKVGLEATYPPFESYDSNKNIVGLDPDFAALIAKDLNAKPQLID
TKFTSLILGLGKKYDAVISGLYVTPERLKQADAIPYAMTGASIIALKNSNILPKTENDLCGVKVGLQQGTAWVASLKKHS
EAYCIKNGKPAIQVMEFPTAPEVSQALMSRNVSAQLEIAPAAKILVEKSRGRLVVSSSRLVYPLPLGIYVTKGNTALVSA
IKSSLAKAKANGQYSALIKKYNLEPVH
>Mature_267_residues
MKQTFKNFSCRMALSVAAVTCFSSMSHAAPLLTNGVFKVGLEATYPPFESYDSNKNIVGLDPDFAALIAKDLNAKPQLID
TKFTSLILGLGKKYDAVISGLYVTPERLKQADAIPYAMTGASIIALKNSNILPKTENDLCGVKVGLQQGTAWVASLKKHS
EAYCIKNGKPAIQVMEFPTAPEVSQALMSRNVSAQLEIAPAAKILVEKSRGRLVVSSSRLVYPLPLGIYVTKGNTALVSA
IKSSLAKAKANGQYSALIKKYNLEPVH

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=229, Percent_Identity=32.3144104803493, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788228, Length=255, Percent_Identity=29.4117647058824, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787088, Length=258, Percent_Identity=27.906976744186, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1788648, Length=236, Percent_Identity=28.3898305084746, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788649, Length=235, Percent_Identity=27.2340425531915, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28681; Mature: 28681

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: PS00579 RIBOSOMAL_L29

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQTFKNFSCRMALSVAAVTCFSSMSHAAPLLTNGVFKVGLEATYPPFESYDSNKNIVGL
CCCCHHCCHHHHHHHHHHHHHHHHHHHCCHHHHCCEEEECCCCCCCCCCCCCCCCCEEEE
DPDFAALIAKDLNAKPQLIDTKFTSLILGLGKKYDAVISGLYVTPERLKQADAIPYAMTG
CCCHHHHHHHHCCCCCCEEHHHHHHHHHHCCCHHHHHHHCEEECHHHHHHHCCCCEEECC
ASIIALKNSNILPKTENDLCGVKVGLQQGTAWVASLKKHSEAYCIKNGKPAIQVMEFPTA
CEEEEEECCCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCEEEEECCCCEEEEEECCCC
PEVSQALMSRNVSAQLEIAPAAKILVEKSRGRLVVSSSRLVYPLPLGIYVTKGNTALVSA
HHHHHHHHHCCCCEEEEECCCEEEEEECCCCEEEEECCCEEEEECEEEEEECCCHHHHHH
IKSSLAKAKANGQYSALIKKYNLEPVH
HHHHHHHHHCCCHHEEHHHHCCCCCCC
>Mature Secondary Structure
MKQTFKNFSCRMALSVAAVTCFSSMSHAAPLLTNGVFKVGLEATYPPFESYDSNKNIVGL
CCCCHHCCHHHHHHHHHHHHHHHHHHHCCHHHHCCEEEECCCCCCCCCCCCCCCCCEEEE
DPDFAALIAKDLNAKPQLIDTKFTSLILGLGKKYDAVISGLYVTPERLKQADAIPYAMTG
CCCHHHHHHHHCCCCCCEEHHHHHHHHHHCCCHHHHHHHCEEECHHHHHHHCCCCEEECC
ASIIALKNSNILPKTENDLCGVKVGLQQGTAWVASLKKHSEAYCIKNGKPAIQVMEFPTA
CEEEEEECCCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCEEEEECCCCEEEEEECCCC
PEVSQALMSRNVSAQLEIAPAAKILVEKSRGRLVVSSSRLVYPLPLGIYVTKGNTALVSA
HHHHHHHHHCCCCEEEEECCCEEEEEECCCCEEEEECCCEEEEECEEEEEECCCHHHHHH
IKSSLAKAKANGQYSALIKKYNLEPVH
HHHHHHHHHCCCHHEEHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]