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The map label for this gene is pcbC [H]

Identifier: 50084479

GI number: 50084479

Start: 1297694

End: 1298770

Strand: Direct

Name: pcbC [H]

Synonym: ACIAD1296

Alternate gene names: 50084479

Gene position: 1297694-1298770 (Clockwise)

Preceding gene: 50084478

Following gene: 50084480

Centisome position: 36.06

GC content: 42.43

Gene sequence:

>1077_bases
ATGACCAAGGTTAAAGGCGCTTTTACTGAACTACCCGTGATTAATATACAGGGCTTAAATAGCGAAAATCTTGCCGAGCG
ACAAGCCGTTGCAGATGCCATTGGACAGGCATCGCGAAAGGTTGGATTTTTCTATATCACTGGTCATGGTATTGCACCAG
AGTTGATCGCAGGTGTGCGGGCAGCAGCAAAAAAATTCTTCGCTTTGCCAATGCAAGAAAAAATGAATTACTACATTGGC
CAATCGAAAAGTCATAAGGGTTATGTTCCCGAAGGTGAAGAGATTTATGGTTCTGGAAAACCAGATTTAAAAGAGGCATT
TGATGTCGGCTTTCAGGCTCCAGAGGATCATCCTTTTGTCGTGTCCAAAACCCCTCTGATTGGTGCAAATGAATGGCCAG
AACTTGCAGAATTTAAGTCACAAGTTTTGGATTATTATCAGGCAGTTTTTGCTTTAGGACATCGTTTATTTGATGCTTTT
GCACTGGCACTTGGTTTGCCAGAAGGTTATTTCAAGTCAATGGTGACTTGCCCGCCATCAAAACTACGTCTGATTCACTA
TCCTTTTGACGCAACAGCGGAAGATAAACCAGGCATTGGTGCACATACTGATTATGAATGTTTCACACTATTGCTTGCAG
ACCAGCCAGGTTTAGAAGTGCTCAATGAAGATAGAGTCTGGATTGATGCGCCGCCATCTAAAGATATCAATGGTGAAGAA
GCTTTTGTCATTAATATTGGTGACATGCTGGAGGTCTTGAGTGCTGGCACCTTTATTGCTACTGCACACCGCGTTCGCAA
AGTACCCCAAGAGCGTTATTCATTTCCACTGTTCTTTGCTTGTGATTATCACACGCTAATTCGCCCATTACCGCATTTCT
TAAAAGCAAACGAGGCAGATCAATATAAAGAGCTAAGTATTGGTGAACATATGTGGAGTCAGGCATTACAAACCTATCGT
TACTTACGTGAAAAAGTTGCCAAGGGTGAGTTGCAATTATCAGAAAGGGCGAGAGCAACCCATACTTTTGGTCACCTGAA
ACAACAAGCAAAGCAAAAAACCACTAGCAATTTGTGA

Upstream 100 bases:

>100_bases
ACCTGTGGCTGATTTGTGGTCGCTTAATGCATGGATGTTGCATGAGGAACAACAGCATTTTAACGAATTTTTATCTTCAA
TGAAAAAGGAGGGCAACTAA

Downstream 100 bases:

>100_bases
GGATTGATTTATGAAACAGACCTTTAAAAATTTTTCATGTCGGATGGCTTTATCTGTCGCCGCAGTCACATGCTTTTCAT
CAATGAGCCATGCTGCACCA

Product: oxidoreductase

Products: NA

Alternate protein names: IPNS [H]

Number of amino acids: Translated: 358; Mature: 357

Protein sequence:

>358_residues
MTKVKGAFTELPVINIQGLNSENLAERQAVADAIGQASRKVGFFYITGHGIAPELIAGVRAAAKKFFALPMQEKMNYYIG
QSKSHKGYVPEGEEIYGSGKPDLKEAFDVGFQAPEDHPFVVSKTPLIGANEWPELAEFKSQVLDYYQAVFALGHRLFDAF
ALALGLPEGYFKSMVTCPPSKLRLIHYPFDATAEDKPGIGAHTDYECFTLLLADQPGLEVLNEDRVWIDAPPSKDINGEE
AFVINIGDMLEVLSAGTFIATAHRVRKVPQERYSFPLFFACDYHTLIRPLPHFLKANEADQYKELSIGEHMWSQALQTYR
YLREKVAKGELQLSERARATHTFGHLKQQAKQKTTSNL

Sequences:

>Translated_358_residues
MTKVKGAFTELPVINIQGLNSENLAERQAVADAIGQASRKVGFFYITGHGIAPELIAGVRAAAKKFFALPMQEKMNYYIG
QSKSHKGYVPEGEEIYGSGKPDLKEAFDVGFQAPEDHPFVVSKTPLIGANEWPELAEFKSQVLDYYQAVFALGHRLFDAF
ALALGLPEGYFKSMVTCPPSKLRLIHYPFDATAEDKPGIGAHTDYECFTLLLADQPGLEVLNEDRVWIDAPPSKDINGEE
AFVINIGDMLEVLSAGTFIATAHRVRKVPQERYSFPLFFACDYHTLIRPLPHFLKANEADQYKELSIGEHMWSQALQTYR
YLREKVAKGELQLSERARATHTFGHLKQQAKQKTTSNL
>Mature_357_residues
TKVKGAFTELPVINIQGLNSENLAERQAVADAIGQASRKVGFFYITGHGIAPELIAGVRAAAKKFFALPMQEKMNYYIGQ
SKSHKGYVPEGEEIYGSGKPDLKEAFDVGFQAPEDHPFVVSKTPLIGANEWPELAEFKSQVLDYYQAVFALGHRLFDAFA
LALGLPEGYFKSMVTCPPSKLRLIHYPFDATAEDKPGIGAHTDYECFTLLLADQPGLEVLNEDRVWIDAPPSKDINGEEA
FVINIGDMLEVLSAGTFIATAHRVRKVPQERYSFPLFFACDYHTLIRPLPHFLKANEADQYKELSIGEHMWSQALQTYRY
LREKVAKGELQLSERARATHTFGHLKQQAKQKTTSNL

Specific function: Removes, in the presence of oxygen, 4 hydrogen atoms from delta-L-(alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) to form the azetidinone and thiazolidine rings of isopenicillin [H]

COG id: COG3491

COG function: function code R; Isopenicillin N synthase and related dioxygenases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002057
- InterPro:   IPR002283
- InterPro:   IPR005123 [H]

Pfam domain/function: PF03171 2OG-FeII_Oxy [H]

EC number: =1.21.3.1 [H]

Molecular weight: Translated: 40046; Mature: 39915

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKVKGAFTELPVINIQGLNSENLAERQAVADAIGQASRKVGFFYITGHGIAPELIAGVR
CCCCCCCHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHCEEEEEEECCCCCHHHHHHHH
AAAKKFFALPMQEKMNYYIGQSKSHKGYVPEGEEIYGSGKPDLKEAFDVGFQAPEDHPFV
HHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCHHHHHHCCCCCCCCCCEE
VSKTPLIGANEWPELAEFKSQVLDYYQAVFALGHRLFDAFALALGLPEGYFKSMVTCPPS
EECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCH
KLRLIHYPFDATAEDKPGIGAHTDYECFTLLLADQPGLEVLNEDRVWIDAPPSKDINGEE
HEEEEECCCCCCCCCCCCCCCCCCHHHEEEEEECCCCCHHHCCCCEEEECCCCCCCCCCC
AFVINIGDMLEVLSAGTFIATAHRVRKVPQERYSFPLFFACDYHTLIRPLPHFLKANEAD
EEEEEHHHHHHHHHCCHHHHHHHHHHHCHHHHCCCCEEEECCHHHHHHHHHHHHCCCCCH
QYKELSIGEHMWSQALQTYRYLREKVAKGELQLSERARATHTFGHLKQQAKQKTTSNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TKVKGAFTELPVINIQGLNSENLAERQAVADAIGQASRKVGFFYITGHGIAPELIAGVR
CCCCCCHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHCEEEEEEECCCCCHHHHHHHH
AAAKKFFALPMQEKMNYYIGQSKSHKGYVPEGEEIYGSGKPDLKEAFDVGFQAPEDHPFV
HHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCHHHHHHCCCCCCCCCCEE
VSKTPLIGANEWPELAEFKSQVLDYYQAVFALGHRLFDAFALALGLPEGYFKSMVTCPPS
EECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCH
KLRLIHYPFDATAEDKPGIGAHTDYECFTLLLADQPGLEVLNEDRVWIDAPPSKDINGEE
HEEEEECCCCCCCCCCCCCCCCCCHHHEEEEEECCCCCHHHCCCCEEEECCCCCCCCCCC
AFVINIGDMLEVLSAGTFIATAHRVRKVPQERYSFPLFFACDYHTLIRPLPHFLKANEAD
EEEEEHHHHHHHHHCCHHHHHHHHHHHCHHHHCCCCEEEECCHHHHHHHHHHHHCCCCCH
QYKELSIGEHMWSQALQTYRYLREKVAKGELQLSERARATHTFGHLKQQAKQKTTSNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1901702 [H]