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The map label for this gene is thiE [H]

Identifier: 50084389

GI number: 50084389

Start: 1200906

End: 1201541

Strand: Direct

Name: thiE [H]

Synonym: ACIAD1200

Alternate gene names: 50084389

Gene position: 1200906-1201541 (Clockwise)

Preceding gene: 50084388

Following gene: 50084390

Centisome position: 33.37

GC content: 42.45

Gene sequence:

>636_bases
ATGCGCGGTCTATATCTCATTACCAATGATGATCCACTTGAGCTGTTATTAGACAAATTAGAAATCGCATTATCCACTGG
ACATATCGCGATTTTGCAGTATCGGCGTAAAAAAGTGCCTAAGACCGATCAAGCTTTTGAAGTCGAACAAATCAAGGTGC
TATGTGAAGCCTATCAGGTTCCTTTTGTCATTAACGATGATCTGGTATTGGCTGCTCAATTTGATTTAGGTGTCCATCTT
GGGCAGTCTGATGGAGAAATCAGTGATGCTGTAAACAGATTAACTTCTGGTGCTGTCATTGGTCGTACCTGTTTAAATTC
GCTCGTGTTGGCAGAGAAAGCCATTGCTGATGGTGCAACCTATATTGCTTTCGGAGCAGTCTATGCAACAGCGACTAAAC
CAGAGGCGGGGAATGTAGGAATTGAAGTGATTAAGCAGGCTCGACAAAAATTTAATACACCCATCTGTGCGATTGGTGGT
TTAACCGTTGAAAACTCGAAGGTCGTCATTGAAAGTGGTGCTGACCTTTGCGCAGTAATTAGTGATATATTAGGTCGTTC
AGTTCAGGATATTCCTACGCGAATCAATGCGTGGGCTGCTTTATTTGCAGAGACTTCATCTGCTGTAAATGTATAG

Upstream 100 bases:

>100_bases
TTATTCGGGTTTTTTTTATGATTAAAATACAATTGCTATAAAAATCAGTTGTGTCGAATTTGTTATACTGTACTTATCAG
AAATGTCGAGAATTGAGTGA

Downstream 100 bases:

>100_bases
TTAAAGTTATATAGATATCGAGAGTATTCATGAGTTTATCTCCAAAACAAGTGCAATTATTTGAGCAAGCCAGCAAACAT
ATTCCAGGGGGTGTGAACTC

Product: thiamin-phosphate pyrophosphorylase (ThiE)

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MRGLYLITNDDPLELLLDKLEIALSTGHIAILQYRRKKVPKTDQAFEVEQIKVLCEAYQVPFVINDDLVLAAQFDLGVHL
GQSDGEISDAVNRLTSGAVIGRTCLNSLVLAEKAIADGATYIAFGAVYATATKPEAGNVGIEVIKQARQKFNTPICAIGG
LTVENSKVVIESGADLCAVISDILGRSVQDIPTRINAWAALFAETSSAVNV

Sequences:

>Translated_211_residues
MRGLYLITNDDPLELLLDKLEIALSTGHIAILQYRRKKVPKTDQAFEVEQIKVLCEAYQVPFVINDDLVLAAQFDLGVHL
GQSDGEISDAVNRLTSGAVIGRTCLNSLVLAEKAIADGATYIAFGAVYATATKPEAGNVGIEVIKQARQKFNTPICAIGG
LTVENSKVVIESGADLCAVISDILGRSVQDIPTRINAWAALFAETSSAVNV
>Mature_211_residues
MRGLYLITNDDPLELLLDKLEIALSTGHIAILQYRRKKVPKTDQAFEVEQIKVLCEAYQVPFVINDDLVLAAQFDLGVHL
GQSDGEISDAVNRLTSGAVIGRTCLNSLVLAEKAIADGATYIAFGAVYATATKPEAGNVGIEVIKQARQKFNTPICAIGG
LTVENSKVVIESGADLCAVISDILGRSVQDIPTRINAWAALFAETSSAVNV

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 22558; Mature: 22558

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGLYLITNDDPLELLLDKLEIALSTGHIAILQYRRKKVPKTDQAFEVEQIKVLCEAYQV
CCCEEEEECCCHHHHHHHHHHHHHCCCCCEEHHHHHHCCCCCCCHHHHHHHHHHHHHHCC
PFVINDDLVLAAQFDLGVHLGQSDGEISDAVNRLTSGAVIGRTCLNSLVLAEKAIADGAT
CEEECCCEEEEEEECCEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCH
YIAFGAVYATATKPEAGNVGIEVIKQARQKFNTPICAIGGLTVENSKVVIESGADLCAVI
HEEHHHEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEECCEEECCCEEEEECCCHHHHHH
SDILGRSVQDIPTRINAWAALFAETSSAVNV
HHHHCCCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRGLYLITNDDPLELLLDKLEIALSTGHIAILQYRRKKVPKTDQAFEVEQIKVLCEAYQV
CCCEEEEECCCHHHHHHHHHHHHHCCCCCEEHHHHHHCCCCCCCHHHHHHHHHHHHHHCC
PFVINDDLVLAAQFDLGVHLGQSDGEISDAVNRLTSGAVIGRTCLNSLVLAEKAIADGAT
CEEECCCEEEEEEECCEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCH
YIAFGAVYATATKPEAGNVGIEVIKQARQKFNTPICAIGGLTVENSKVVIESGADLCAVI
HEEHHHEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEECCEEECCCEEEEECCCHHHHHH
SDILGRSVQDIPTRINAWAALFAETSSAVNV
HHHHCCCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA