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The map label for this gene is rluE [H]

Identifier: 50084378

GI number: 50084378

Start: 1187492

End: 1188286

Strand: Reverse

Name: rluE [H]

Synonym: ACIAD1189

Alternate gene names: 50084378

Gene position: 1188286-1187492 (Counterclockwise)

Preceding gene: 50084384

Following gene: 50084377

Centisome position: 33.02

GC content: 42.52

Gene sequence:

>795_bases
ATGAAAATCGTTGTATTCAACAAACCTTATGATGTCCTATCTCAATTTCGTGAAGATAGCGCGCATTTGACTGTTTCTAG
TTTTATTGCAGATCCTGAACTTCGTATTGCAGGACGTTTAGACTTGGATTCTGAAGGCTTAATGTTTTTGACAGACCATG
GTGGTCTGAATCAATACATTACCCACCCAAGTAATAAAAAATATAAGACCTATCTGGTTCAGGTCGATGGTGACATCACC
GAAGATGCCTTGGAAAAATTGCGTAAAGGTGTTGAGCTTGCTGATGGCCTTACCCTACCCGCCCAGGCAACAAAAGTTGA
AGAACCAGAATGGCTATGGGAGCGAAATCCACCAGTCCGCTTTCGTGCCAGCATTCCCACCTCATGGGTTGAAATTTCAA
TCTGTGAAGGCCGCAATCGTCAGGTTCGCCGCATGACTGCCACGGTAGGATTTCCAACATTGCGTTTAATCCGTACACAA
ATCGGTTCAATTAATTTGGTAAAATTAGGCTTACAACCTGGAGAGCAAAAAGAAATTGAACCTTTACTATATCCAGATTT
TAAAGATGTGCCTGAAGACAAACCTTATCGCTCACGTTCATATGTAAAAAAACCAGGTGGAACTGGCGGTAAGCCAATCC
ATCATAAAAATAAAGATGGTGACAAGAAAGCGTCAGATAAGAAAAAATCTGGTACCAAACGTATCTGGCAAATGGATGAA
GATGAAAAGCCACGTCGTAAGACCAATGGTACAACTCGTCCAAACACCAAAAGCCGTGGACGCAGAGCCCGCTAA

Upstream 100 bases:

>100_bases
TCGTTGTTGGTGAGATTTTGAGCTAAAATAGCAACATTTCTCATTTAAATAAAAACATCTCTGTTTTCAAATACACTGAA
AACCAAAGCAGAATTTTTTT

Downstream 100 bases:

>100_bases
TCAATTGATAGATTACATTTTTTAAACCTATGATTTTTAATCAATTGGTTAACATAATGGTTTCTAAATTCTGGAGACCA
TTTAATGCTTAAAATATTAC

Product: pseudouridine synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKIVVFNKPYDVLSQFREDSAHLTVSSFIADPELRIAGRLDLDSEGLMFLTDHGGLNQYITHPSNKKYKTYLVQVDGDIT
EDALEKLRKGVELADGLTLPAQATKVEEPEWLWERNPPVRFRASIPTSWVEISICEGRNRQVRRMTATVGFPTLRLIRTQ
IGSINLVKLGLQPGEQKEIEPLLYPDFKDVPEDKPYRSRSYVKKPGGTGGKPIHHKNKDGDKKASDKKKSGTKRIWQMDE
DEKPRRKTNGTTRPNTKSRGRRAR

Sequences:

>Translated_264_residues
MKIVVFNKPYDVLSQFREDSAHLTVSSFIADPELRIAGRLDLDSEGLMFLTDHGGLNQYITHPSNKKYKTYLVQVDGDIT
EDALEKLRKGVELADGLTLPAQATKVEEPEWLWERNPPVRFRASIPTSWVEISICEGRNRQVRRMTATVGFPTLRLIRTQ
IGSINLVKLGLQPGEQKEIEPLLYPDFKDVPEDKPYRSRSYVKKPGGTGGKPIHHKNKDGDKKASDKKKSGTKRIWQMDE
DEKPRRKTNGTTRPNTKSRGRRAR
>Mature_264_residues
MKIVVFNKPYDVLSQFREDSAHLTVSSFIADPELRIAGRLDLDSEGLMFLTDHGGLNQYITHPSNKKYKTYLVQVDGDIT
EDALEKLRKGVELADGLTLPAQATKVEEPEWLWERNPPVRFRASIPTSWVEISICEGRNRQVRRMTATVGFPTLRLIRTQ
IGSINLVKLGLQPGEQKEIEPLLYPDFKDVPEDKPYRSRSYVKKPGGTGGKPIHHKNKDGDKKASDKKKSGTKRIWQMDE
DEKPRRKTNGTTRPNTKSRGRRAR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=178, Percent_Identity=55.6179775280899, Blast_Score=196, Evalue=1e-51,
Organism=Escherichia coli, GI1788510, Length=179, Percent_Identity=34.0782122905028, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1787524, Length=187, Percent_Identity=32.0855614973262, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790453, Length=181, Percent_Identity=32.0441988950276, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 30108; Mature: 30108

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVVFNKPYDVLSQFREDSAHLTVSSFIADPELRIAGRLDLDSEGLMFLTDHGGLNQYI
CEEEEECCCHHHHHHHHCCCCEEEEEHHHCCCCEEEEEEECCCCCCEEEEECCCCCCHHE
THPSNKKYKTYLVQVDGDITEDALEKLRKGVELADGLTLPAQATKVEEPEWLWERNPPVR
ECCCCCCEEEEEEEECCCCCHHHHHHHHHCCCHHCCCCCCCCCCCCCCCHHHHCCCCCEE
FRASIPTSWVEISICEGRNRQVRRMTATVGFPTLRLIRTQIGSINLVKLGLQPGEQKEIE
EEECCCCCEEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHCCEEEEEECCCCCCCCCCC
PLLYPDFKDVPEDKPYRSRSYVKKPGGTGGKPIHHKNKDGDKKASDKKKSGTKRIWQMDE
CEECCCCCCCCCCCCCCCCHHEECCCCCCCCCCCCCCCCCCCHHCCHHHCCCHHHHCCCC
DEKPRRKTNGTTRPNTKSRGRRAR
CCCCHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKIVVFNKPYDVLSQFREDSAHLTVSSFIADPELRIAGRLDLDSEGLMFLTDHGGLNQYI
CEEEEECCCHHHHHHHHCCCCEEEEEHHHCCCCEEEEEEECCCCCCEEEEECCCCCCHHE
THPSNKKYKTYLVQVDGDITEDALEKLRKGVELADGLTLPAQATKVEEPEWLWERNPPVR
ECCCCCCEEEEEEEECCCCCHHHHHHHHHCCCHHCCCCCCCCCCCCCCCHHHHCCCCCEE
FRASIPTSWVEISICEGRNRQVRRMTATVGFPTLRLIRTQIGSINLVKLGLQPGEQKEIE
EEECCCCCEEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHCCEEEEEECCCCCCCCCCC
PLLYPDFKDVPEDKPYRSRSYVKKPGGTGGKPIHHKNKDGDKKASDKKKSGTKRIWQMDE
CEECCCCCCCCCCCCCCCCHHEECCCCCCCCCCCCCCCCCCCHHCCHHHCCCHHHHCCCC
DEKPRRKTNGTTRPNTKSRGRRAR
CCCCHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA