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The map label for this gene is cobQ

Identifier: 50084270

GI number: 50084270

Start: 1066587

End: 1067984

Strand: Reverse

Name: cobQ

Synonym: ACIAD1071

Alternate gene names: 50084270

Gene position: 1067984-1066587 (Counterclockwise)

Preceding gene: 50084273

Following gene: 50084269

Centisome position: 29.68

GC content: 39.13

Gene sequence:

>1398_bases
ATGAGACATATTTCGATTTTTGGAACCTCCAGCGATGCTGGAAAATCGACTCTCACTTTTGTGATTGCAAAAATTTTACA
GCGCGCTGGTATTCGAGTTGCGCCGTTTAAAGCACAAAATGTCTCCAATAATGCACGTGTCTGTGACGATGGCAGTGAAA
TTGCAATCGCCCAATCCTTTCAGGCTGAAGTCTTAGCAATTCCAACGTCTTATCACCTCAATCCTATATTGCTTAAATCT
GGCATAAACGGCCAAGCCTCTGTCATCGTAGAAGGTCAGGTTATTTCTCAACAAGATGTATTGGAATATTATCGTGATCT
GGACACACTTAAACCCGCAGTACAACGTTGCTTTGAAACTCTGGCAAAAATGTATGATTGCATTGTGGCAGAAGGTGCAG
GTAGTCCAGTTGAACTCAACTTGATGGATAAAGACCTATCCAATATTTTTATTGCCAAACATTATCAAACCAAAATTATT
TTAGTTGCTGATATTGAAAAAGGCGGCGTGTTTGCCTCGGTGTGGGGAACTTACAATTTACTGCCCGCAGATTTACGTAG
CAATGTGATTGGTGTCATCATCAATAAATTTCGTGGTGATCTATCTTTATTTGATGAAGGCATCAGAATTATAGAACAAG
CGTTTAAAATTCCTGTACTCGGCGTTTTACCTTATATGCCGTTCAATCTGGGCTTTGAGGACAATGCCAGTCTACAAAAC
TTTGTACAACAGCCCCGTCAGAAAAAACTCAAGATTGGCGTGATTGCCTATCCGTATATGAGTAACTATAACGACTTTGA
ACCTCTCATTGCAGATGATGAAGTCTTACTGGAATTTATTAACTCTCCTATTTCACTGGAGCATTTTGATGGGGTGATTC
TGCCCGGTTCAAAGCTGGTGATTGAAGATTTACACTGGTTAAAAACAAATGGATTGTTTGAACAGCTTCAACAACGTCGC
AAGCCCATTTTTGCAATTTGCGGGGGCTATGAAATGTTATTTGAACAACTTCATGATCCAGAAAGAATCGAAAATTTAGA
GCCGACCACAGCGACAGGCCTTGGTTTGATCGATGATGAGATTTACTTTGCAAAGAATAAAATCTTAAACAAAGCAGAAT
ACCCAATTTTTGGCCTCAACATTGCTGGCTTTGAAATGCATCACGGTATAAGCCAGAAGTATCCACTGTATTTCCAAAAA
GACAATATTCAAGGCACTTTTATTCATCAAGTCTTTGATCATAATGCGTTTAGAACACAATATTTACGTGCGATTTGTGC
ACAGTATCAGGGCTTTGATTTTCAGTTATACAAAACTGAGCAGATCAATAATTTTATTGAAGCATGTCGAAAGCGGCTGA
ATGTAAAGCTGATTCTTGAGGCAATTCAGGATCAATAA

Upstream 100 bases:

>100_bases
AGATATACTCTATCAAATCACTACGCTGATTTTTTAAATAGCATATTATGCCGTAGCCAACTTACACTGCCTACCCGATT
TTATTGATAAGCATATCAGT

Downstream 100 bases:

>100_bases
TGCTTGGAAAGCAATATTGTCATCATTGGTTTAAATCCAAAGAATCTCGATGAAAGCCTTAAAGCTTAACCAGACATTAA
TGCTTTATTATATAAATGTA

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MRHISIFGTSSDAGKSTLTFVIAKILQRAGIRVAPFKAQNVSNNARVCDDGSEIAIAQSFQAEVLAIPTSYHLNPILLKS
GINGQASVIVEGQVISQQDVLEYYRDLDTLKPAVQRCFETLAKMYDCIVAEGAGSPVELNLMDKDLSNIFIAKHYQTKII
LVADIEKGGVFASVWGTYNLLPADLRSNVIGVIINKFRGDLSLFDEGIRIIEQAFKIPVLGVLPYMPFNLGFEDNASLQN
FVQQPRQKKLKIGVIAYPYMSNYNDFEPLIADDEVLLEFINSPISLEHFDGVILPGSKLVIEDLHWLKTNGLFEQLQQRR
KPIFAICGGYEMLFEQLHDPERIENLEPTTATGLGLIDDEIYFAKNKILNKAEYPIFGLNIAGFEMHHGISQKYPLYFQK
DNIQGTFIHQVFDHNAFRTQYLRAICAQYQGFDFQLYKTEQINNFIEACRKRLNVKLILEAIQDQ

Sequences:

>Translated_465_residues
MRHISIFGTSSDAGKSTLTFVIAKILQRAGIRVAPFKAQNVSNNARVCDDGSEIAIAQSFQAEVLAIPTSYHLNPILLKS
GINGQASVIVEGQVISQQDVLEYYRDLDTLKPAVQRCFETLAKMYDCIVAEGAGSPVELNLMDKDLSNIFIAKHYQTKII
LVADIEKGGVFASVWGTYNLLPADLRSNVIGVIINKFRGDLSLFDEGIRIIEQAFKIPVLGVLPYMPFNLGFEDNASLQN
FVQQPRQKKLKIGVIAYPYMSNYNDFEPLIADDEVLLEFINSPISLEHFDGVILPGSKLVIEDLHWLKTNGLFEQLQQRR
KPIFAICGGYEMLFEQLHDPERIENLEPTTATGLGLIDDEIYFAKNKILNKAEYPIFGLNIAGFEMHHGISQKYPLYFQK
DNIQGTFIHQVFDHNAFRTQYLRAICAQYQGFDFQLYKTEQINNFIEACRKRLNVKLILEAIQDQ
>Mature_465_residues
MRHISIFGTSSDAGKSTLTFVIAKILQRAGIRVAPFKAQNVSNNARVCDDGSEIAIAQSFQAEVLAIPTSYHLNPILLKS
GINGQASVIVEGQVISQQDVLEYYRDLDTLKPAVQRCFETLAKMYDCIVAEGAGSPVELNLMDKDLSNIFIAKHYQTKII
LVADIEKGGVFASVWGTYNLLPADLRSNVIGVIINKFRGDLSLFDEGIRIIEQAFKIPVLGVLPYMPFNLGFEDNASLQN
FVQQPRQKKLKIGVIAYPYMSNYNDFEPLIADDEVLLEFINSPISLEHFDGVILPGSKLVIEDLHWLKTNGLFEQLQQRR
KPIFAICGGYEMLFEQLHDPERIENLEPTTATGLGLIDDEIYFAKNKILNKAEYPIFGLNIAGFEMHHGISQKYPLYFQK
DNIQGTFIHQVFDHNAFRTQYLRAICAQYQGFDFQLYKTEQINNFIEACRKRLNVKLILEAIQDQ

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBQ_ACIAD (Q43989)

Other databases:

- EMBL:   Z46863
- EMBL:   CR543861
- RefSeq:   YP_045780.1
- ProteinModelPortal:   Q43989
- STRING:   Q43989
- GeneID:   2877936
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD1071
- NMPDR:   fig|62977.3.peg.1219
- eggNOG:   COG1492
- HOGENOM:   HBG302271
- OMA:   GVDVAPF
- ProtClustDB:   CLSK2762703
- BioCyc:   ASP62977:ACIAD1071-MONOMER
- HAMAP:   MF_00028
- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698
- TIGRFAMs:   TIGR00313

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3

EC number: 6.3.5.10

Molecular weight: Translated: 52573; Mature: 52573

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS51274 GATASE_COBBQ

Important sites: ACT_SITE 327-327 ACT_SITE 409-409

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHISIFGTSSDAGKSTLTFVIAKILQRAGIRVAPFKAQNVSNNARVCDDGSEIAIAQSF
CCEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCEECCCCCCEEEEECC
QAEVLAIPTSYHLNPILLKSGINGQASVIVEGQVISQQDVLEYYRDLDTLKPAVQRCFET
CCEEEEECCCCCCCEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LAKMYDCIVAEGAGSPVELNLMDKDLSNIFIAKHYQTKIILVADIEKGGVFASVWGTYNL
HHHHHHHEEECCCCCCEEEEEECCCCCCEEEEEECCEEEEEEEECCCCCEEEEECCCCCC
LPADLRSNVIGVIINKFRGDLSLFDEGIRIIEQAFKIPVLGVLPYMPFNLGFEDNASLQN
CCHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCHHHH
FVQQPRQKKLKIGVIAYPYMSNYNDFEPLIADDEVLLEFINSPISLEHFDGVILPGSKLV
HHHCHHHCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCEECCCCEEECCCHHH
IEDLHWLKTNGLFEQLQQRRKPIFAICGGYEMLFEQLHDPERIENLEPTTATGLGLIDDE
HHHHHHHHHCHHHHHHHHHCCCEEEEECCHHHHHHHCCCHHHHCCCCCCCCCCCCEECCH
IYFAKNKILNKAEYPIFGLNIAGFEMHHGISQKYPLYFQKDNIQGTFIHQVFDHNAFRTQ
HHHHHHHHCCCCCCCEEEEEEECCHHHCCCCCCCCEEEEECCCCEEEHHHHHCCCHHHHH
YLRAICAQYQGFDFQLYKTEQINNFIEACRKRLNVKLILEAIQDQ
HHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure
MRHISIFGTSSDAGKSTLTFVIAKILQRAGIRVAPFKAQNVSNNARVCDDGSEIAIAQSF
CCEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCEECCCCCCEEEEECC
QAEVLAIPTSYHLNPILLKSGINGQASVIVEGQVISQQDVLEYYRDLDTLKPAVQRCFET
CCEEEEECCCCCCCEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LAKMYDCIVAEGAGSPVELNLMDKDLSNIFIAKHYQTKIILVADIEKGGVFASVWGTYNL
HHHHHHHEEECCCCCCEEEEEECCCCCCEEEEEECCEEEEEEEECCCCCEEEEECCCCCC
LPADLRSNVIGVIINKFRGDLSLFDEGIRIIEQAFKIPVLGVLPYMPFNLGFEDNASLQN
CCHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCHHHH
FVQQPRQKKLKIGVIAYPYMSNYNDFEPLIADDEVLLEFINSPISLEHFDGVILPGSKLV
HHHCHHHCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCEECCCCEEECCCHHH
IEDLHWLKTNGLFEQLQQRRKPIFAICGGYEMLFEQLHDPERIENLEPTTATGLGLIDDE
HHHHHHHHHCHHHHHHHHHCCCEEEEECCHHHHHHHCCCHHHHCCCCCCCCCCCCEECCH
IYFAKNKILNKAEYPIFGLNIAGFEMHHGISQKYPLYFQKDNIQGTFIHQVFDHNAFRTQ
HHHHHHHHCCCCCCCEEEEEEECCHHHCCCCCCCCEEEEECCCCEEEHHHHHCCCHHHHH
YLRAICAQYQGFDFQLYKTEQINNFIEACRKRLNVKLILEAIQDQ
HHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9074511