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The map label for this gene is betT [H]

Identifier: 50084214

GI number: 50084214

Start: 1000935

End: 1002515

Strand: Direct

Name: betT [H]

Synonym: ACIAD1011

Alternate gene names: 50084214

Gene position: 1000935-1002515 (Clockwise)

Preceding gene: 50084207

Following gene: 50084215

Centisome position: 27.81

GC content: 43.39

Gene sequence:

>1581_bases
ATGTGGTCAAAACGTGACGAACAGAAAACCTATCCACCCATTCGCCTGAATCCATTTGTATTCTGGAGTTCAGCGATCAG
TATCAGTATTTTCGGGATGCTCTTTGTCTTGTTTCCCGAAACCAGCCAGCACGGATTGACCTGGATTCAGCAACAGGTCA
ATCAGTTGTTTGGTTGGTATTATATGCTGGTGATCATATTGAGCCTCGGATTTGTTGCTTGGCTTGCTTTTTCTCAAGTG
GGGAATATTCCCTTGGGTAAGGCACAAGACAAACCCGAATTTGGTTATCTGGTATGGACCTCCATGCTGTTCTCAGCAGG
GATTGGTATTGCACTTTTATATTATGGTGTTGCCGAGCCAGTTGACCATTTTTTACGGCCACCTGAAGGGCAGGGCGGAA
CAGTAGAAGCAGCACAGAATGCCATGATGTATAGTTTTTTGCATTGGGGTATTCATGGCTGGGTACTGTATGCCTTAGTG
GGTGTAACTTTAGGTTATTTTGCATTTCGTCGTGATTTACCCTTGGCATTACGTTCTGCGCTTTATCCTATTTTTGGAGA
GCGAATTCACGGGCTTGTCGGGCATATGGTGGATGGTTTTGGTATTTTAGCCACGATCATTTCGCTGGTGACGAATTTGG
GTATCGGTGCATTGGTCATGATTTCTGGAATCAGCTATCTGTTCCCAGATCTTCCAAATACCTCTAGCACTTTGGTGGTC
ACGGTCATCATGATGATGCTGGTTGCGACACTAACCACGGTGATTGGGATTGAAAAGGGGCTGGCGTGGCTGTCCAGAAT
TAATCTGCGCTTATTATATCTGTTGTTATTGTTTGTATTTTTGACAGGTCCAACCAATCATCTGCTGAATGGTCTGGTGC
AAAATACAGGTGATTATTTGAGTCATTTTGTACAGAAAAGCTTTGATCTTTATTTGTATGACAAAAATGCAACAGGCTGG
CTAGCCTCATGGACGATTTTTTATTGGGCATGGTGGATTGCATGGGCACCTTTTGTGGGAATGTTTATTGCGCGTATTTC
CAAAGGTCGTACTATTCGCGAAGTCGTGCTTGGCGTATGTTTGATTCCACTGGGTTTTACATTGGCGTGGATTTCTATTT
TTGGAAATACTGCGATTGATTTAATCCTGAATCATGGGCAGCAGATAATTGGTAGCCTGGTAATTCAAGATCCAGCATTG
TCATTGTTCAAGTTACTTGAATATTTACCATTTCATCCCTATGTGGCGGGAATTGTTGTGGTGATTTGTTTCGTTTTATT
TTTAACCCCAGTAGGCTCTGGTACACTCATGATCGCTAATTTGTCATCTCAAGGTGGCAGCAGTGATAGTGATTCGCCTA
TTTGGTTACGCGTTTTTTGGTCGATTGCGATTACCATTGTGAGTATTGGTTTGTTGCTGGCGGGCAGCTTCAGTGCAATG
CAAAGTGCGGTTGTGCTGTGTGGTTTACCATTTTCAGTGATTTTACTGTTGTACATGTTTGGTCTTGCCAAAGCATTAAA
ACAGGAGACACAGCAACCAGTGGTGGAGTCGCATACTACTGAGACTTCAGGTTCAGATTAG

Upstream 100 bases:

>100_bases
TAAAAGCTAGCATCCAGCGTTCTCATTGAATGGAATGTCATGAGGGATACTCGCCAGCCACATTGATTATTAGGCGTCAC
ATCTTGCAAGGAGCAATGTT

Downstream 100 bases:

>100_bases
ACCAAAAAAATAGCCTAACCCAAATGACTAAAATCTAATTCTGAATATTTCAGAAAAAGAATATTGATGTCATCTATTTT
GTCAATCTTTAAGCGGCTGT

Product: choline/carnitine/betaine transporter family protein

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 526; Mature: 526

Protein sequence:

>526_residues
MWSKRDEQKTYPPIRLNPFVFWSSAISISIFGMLFVLFPETSQHGLTWIQQQVNQLFGWYYMLVIILSLGFVAWLAFSQV
GNIPLGKAQDKPEFGYLVWTSMLFSAGIGIALLYYGVAEPVDHFLRPPEGQGGTVEAAQNAMMYSFLHWGIHGWVLYALV
GVTLGYFAFRRDLPLALRSALYPIFGERIHGLVGHMVDGFGILATIISLVTNLGIGALVMISGISYLFPDLPNTSSTLVV
TVIMMMLVATLTTVIGIEKGLAWLSRINLRLLYLLLLFVFLTGPTNHLLNGLVQNTGDYLSHFVQKSFDLYLYDKNATGW
LASWTIFYWAWWIAWAPFVGMFIARISKGRTIREVVLGVCLIPLGFTLAWISIFGNTAIDLILNHGQQIIGSLVIQDPAL
SLFKLLEYLPFHPYVAGIVVVICFVLFLTPVGSGTLMIANLSSQGGSSDSDSPIWLRVFWSIAITIVSIGLLLAGSFSAM
QSAVVLCGLPFSVILLLYMFGLAKALKQETQQPVVESHTTETSGSD

Sequences:

>Translated_526_residues
MWSKRDEQKTYPPIRLNPFVFWSSAISISIFGMLFVLFPETSQHGLTWIQQQVNQLFGWYYMLVIILSLGFVAWLAFSQV
GNIPLGKAQDKPEFGYLVWTSMLFSAGIGIALLYYGVAEPVDHFLRPPEGQGGTVEAAQNAMMYSFLHWGIHGWVLYALV
GVTLGYFAFRRDLPLALRSALYPIFGERIHGLVGHMVDGFGILATIISLVTNLGIGALVMISGISYLFPDLPNTSSTLVV
TVIMMMLVATLTTVIGIEKGLAWLSRINLRLLYLLLLFVFLTGPTNHLLNGLVQNTGDYLSHFVQKSFDLYLYDKNATGW
LASWTIFYWAWWIAWAPFVGMFIARISKGRTIREVVLGVCLIPLGFTLAWISIFGNTAIDLILNHGQQIIGSLVIQDPAL
SLFKLLEYLPFHPYVAGIVVVICFVLFLTPVGSGTLMIANLSSQGGSSDSDSPIWLRVFWSIAITIVSIGLLLAGSFSAM
QSAVVLCGLPFSVILLLYMFGLAKALKQETQQPVVESHTTETSGSD
>Mature_526_residues
MWSKRDEQKTYPPIRLNPFVFWSSAISISIFGMLFVLFPETSQHGLTWIQQQVNQLFGWYYMLVIILSLGFVAWLAFSQV
GNIPLGKAQDKPEFGYLVWTSMLFSAGIGIALLYYGVAEPVDHFLRPPEGQGGTVEAAQNAMMYSFLHWGIHGWVLYALV
GVTLGYFAFRRDLPLALRSALYPIFGERIHGLVGHMVDGFGILATIISLVTNLGIGALVMISGISYLFPDLPNTSSTLVV
TVIMMMLVATLTTVIGIEKGLAWLSRINLRLLYLLLLFVFLTGPTNHLLNGLVQNTGDYLSHFVQKSFDLYLYDKNATGW
LASWTIFYWAWWIAWAPFVGMFIARISKGRTIREVVLGVCLIPLGFTLAWISIFGNTAIDLILNHGQQIIGSLVIQDPAL
SLFKLLEYLPFHPYVAGIVVVICFVLFLTPVGSGTLMIANLSSQGGSSDSDSPIWLRVFWSIAITIVSIGLLLAGSFSAM
QSAVVLCGLPFSVILLLYMFGLAKALKQETQQPVVESHTTETSGSD

Specific function: High-affinity uptake of choline driven by a proton- motive force [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=521, Percent_Identity=41.4587332053743, Blast_Score=431, Evalue=1e-122,
Organism=Escherichia coli, GI1788102, Length=469, Percent_Identity=31.3432835820896, Blast_Score=191, Evalue=9e-50,
Organism=Escherichia coli, GI1786224, Length=515, Percent_Identity=26.0194174757282, Blast_Score=172, Evalue=5e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 58181; Mature: 58181

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS01303 BCCT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCC
>Mature Secondary Structure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HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]