Click here to switch to the map view.

The map label for this gene is gph [C]

Identifier: 50084087

GI number: 50084087

Start: 859037

End: 859711

Strand: Direct

Name: gph [C]

Synonym: ACIAD0876

Alternate gene names: 50084087

Gene position: 859037-859711 (Clockwise)

Preceding gene: 50084084

Following gene: 50084088

Centisome position: 23.87

GC content: 40.59

Gene sequence:

>675_bases
GTGAGCGTTGTAAAAAATATTCTGATTGATTTGGATGGAACTTTAACAGACCCTAAAGTGGGCATTACCACTTCGGCTCG
CTATGGGCTAAATAAAGTTGGGTTTTGTGTTGCTGAACATGAAAATATTGACTGGATTATTGGACCACCGCTTAAGGCTT
CTTTGGCAAAAATTATTAATGTTGATGCCAGTCATGACTTGGCAGAGCAGGCATTACAGGGTTATCGGGAGCGTTTTTCT
GTTATTGGCCTATTTGAAAATGAACTGTATCCGCATGTAAGGTCAACGTTACAAGTACTTCAAACTCAAGGTTATCGGTT
ATATCTGGCAACAGCCAAACCACATGTTTATGCCGTGCGTATCTTGGAGCATTTTGATTTGCTCAAATATTTTATTCATC
CCTATGGCAGTGAACTCACAGGTGAGCGAACCAATAAAGGCGACCTGATTCGTTATATTCTTGAGCAAGAGCAATTAGAT
CCTGCAGAATGCATTATGGTGGGTGATCGCGAGCATGATATTTTAGGTGCACGTCGCAATGGAATTGAAACGATTGCAGT
GGAATACGGCTACGGTACTCAGCAAGAGCTAGATCTGGCAACACCAAAGTATCGAATTCAAAGTTTCGATCAGGTTTTGA
AAGTCATCACTGAACATGAATATGATCAAATTTAA

Upstream 100 bases:

>100_bases
CACAGTTATTATCCTTAAAATATTCAATTTGAGTTAGTATAGGCAAGTTTGAATAAACTCTCGACCCTATTTTTTATCTA
GCATTGTTTGAGTGTGATTT

Downstream 100 bases:

>100_bases
TTTATAATGAAAGACAGGTTTACACATTTGAATCTGTCTTTTTATAATCCATTTTTTGGTTTATTTCACAAAATAGCCTT
AATTTTTATTAATATGGATC

Product: hydrolase

Products: NA

Alternate protein names: Nucleoside 5'-monophosphate phosphohydrolase [H]

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MSVVKNILIDLDGTLTDPKVGITTSARYGLNKVGFCVAEHENIDWIIGPPLKASLAKIINVDASHDLAEQALQGYRERFS
VIGLFENELYPHVRSTLQVLQTQGYRLYLATAKPHVYAVRILEHFDLLKYFIHPYGSELTGERTNKGDLIRYILEQEQLD
PAECIMVGDREHDILGARRNGIETIAVEYGYGTQQELDLATPKYRIQSFDQVLKVITEHEYDQI

Sequences:

>Translated_224_residues
MSVVKNILIDLDGTLTDPKVGITTSARYGLNKVGFCVAEHENIDWIIGPPLKASLAKIINVDASHDLAEQALQGYRERFS
VIGLFENELYPHVRSTLQVLQTQGYRLYLATAKPHVYAVRILEHFDLLKYFIHPYGSELTGERTNKGDLIRYILEQEQLD
PAECIMVGDREHDILGARRNGIETIAVEYGYGTQQELDLATPKYRIQSFDQVLKVITEHEYDQI
>Mature_223_residues
SVVKNILIDLDGTLTDPKVGITTSARYGLNKVGFCVAEHENIDWIIGPPLKASLAKIINVDASHDLAEQALQGYRERFSV
IGLFENELYPHVRSTLQVLQTQGYRLYLATAKPHVYAVRILEHFDLLKYFIHPYGSELTGERTNKGDLIRYILEQEQLDP
AECIMVGDREHDILGARRNGIETIAVEYGYGTQQELDLATPKYRIQSFDQVLKVITEHEYDQI

Specific function: Specifically dephosphorylates nucleoside 5'- monophosphates to nucleosides and inorganic phosphate. Displays high activity toward 5'-UMP and 5'-IMP, significant activity against 5'-XMP and 5'-TMP, and low activity against 5'-CMP [H]

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=233, Percent_Identity=27.0386266094421, Blast_Score=81, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.5 [H]

Molecular weight: Translated: 25477; Mature: 25346

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVVKNILIDLDGTLTDPKVGITTSARYGLNKVGFCVAEHENIDWIIGPPLKASLAKIIN
CCHHHHHHHHCCCCCCCCCCCEECCCCCCCCHHEEEEEECCCCCEEECCCHHHHHHHHHC
VDASHDLAEQALQGYRERFSVIGLFENELYPHVRSTLQVLQTQGYRLYLATAKPHVYAVR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHH
ILEHFDLLKYFIHPYGSELTGERTNKGDLIRYILEQEQLDPAECIMVGDREHDILGARRN
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHEEEECCCCCCHHCCCCC
GIETIAVEYGYGTQQELDLATPKYRIQSFDQVLKVITEHEYDQI
CCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SVVKNILIDLDGTLTDPKVGITTSARYGLNKVGFCVAEHENIDWIIGPPLKASLAKIIN
CHHHHHHHHCCCCCCCCCCCEECCCCCCCCHHEEEEEECCCCCEEECCCHHHHHHHHHC
VDASHDLAEQALQGYRERFSVIGLFENELYPHVRSTLQVLQTQGYRLYLATAKPHVYAVR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHH
ILEHFDLLKYFIHPYGSELTGERTNKGDLIRYILEQEQLDPAECIMVGDREHDILGARRN
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHEEEECCCCCCHHCCCCC
GIETIAVEYGYGTQQELDLATPKYRIQSFDQVLKVITEHEYDQI
CCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]