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The map label for this gene is bioD [H]

Identifier: 50084071

GI number: 50084071

Start: 846361

End: 847002

Strand: Direct

Name: bioD [H]

Synonym: ACIAD0859

Alternate gene names: 50084071

Gene position: 846361-847002 (Clockwise)

Preceding gene: 50084070

Following gene: 50084076

Centisome position: 23.52

GC content: 38.32

Gene sequence:

>642_bases
ATGACGGCTTCTGTTTATTTTATTAGTGGCATTGATACAGGTATTGGAAAAACTTATGCCACGGGATATCTTGCAAAGCA
ATGGATTGATCAGGGTATAAAAACCATTACTCAAAAGCTGATTCAGACAGGCAATATTGAAATATCGGAAGATATAGAAA
AGCATCGTGACATTATGCAGACTGGTTTTTTGCCAGAAGATGATGAAAAACTCACCATGCCTGAGATTTTTACTTATCCT
GCATCACCACATCTGGCAACCAAAATGGATGGAAGAGCGCTGGATTTCAATAAAATTGAACAGGCCACTCACATATTGAA
ACAACGTTTTGATGTGGTATTGCTTGAAGGTGCAGGTGGTTTAATGGTGCCGCTGACCACAGATATGCTCACTATTGATT
ACATTGCAGAGCATCAGTTTCCCATCATTCTGGTGAGTTCGGGCCGTTTAGGGAGTATTAATCATACTCTGTTAAGTCTT
GAAGCGATAAAACAGCGTGGCTTGTCACTTTATGCCTTAGCGTATAATTTGAATGATGAGTCGCAAGATACAATCATTTC
TAAAGATACAGCCCAGTATTTAAAACAATATCTTAAGCACTCATTTCCAGAGGCAAAATGGATTGATATTCCTAAGATTT
AA

Upstream 100 bases:

>100_bases
CTTTAGCACAGTTCTATCATGATTATTGTCAGTTTCAGACAGATGATGGTCATTATGAACTGACCTATCATCCTATTTAT
ATCATTGCTCGGAGAACGTC

Downstream 100 bases:

>100_bases
AAAAGACAGACTATGTCTTAGAAAAGGTTTTATTCAATGAATAAAACCTTTTCGTAAATAAAGCATTTTATTTGTAATAT
TTCTGAACTTCCGTGGAAAT

Product: dithiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase [H]

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MTASVYFISGIDTGIGKTYATGYLAKQWIDQGIKTITQKLIQTGNIEISEDIEKHRDIMQTGFLPEDDEKLTMPEIFTYP
ASPHLATKMDGRALDFNKIEQATHILKQRFDVVLLEGAGGLMVPLTTDMLTIDYIAEHQFPIILVSSGRLGSINHTLLSL
EAIKQRGLSLYALAYNLNDESQDTIISKDTAQYLKQYLKHSFPEAKWIDIPKI

Sequences:

>Translated_213_residues
MTASVYFISGIDTGIGKTYATGYLAKQWIDQGIKTITQKLIQTGNIEISEDIEKHRDIMQTGFLPEDDEKLTMPEIFTYP
ASPHLATKMDGRALDFNKIEQATHILKQRFDVVLLEGAGGLMVPLTTDMLTIDYIAEHQFPIILVSSGRLGSINHTLLSL
EAIKQRGLSLYALAYNLNDESQDTIISKDTAQYLKQYLKHSFPEAKWIDIPKI
>Mature_212_residues
TASVYFISGIDTGIGKTYATGYLAKQWIDQGIKTITQKLIQTGNIEISEDIEKHRDIMQTGFLPEDDEKLTMPEIFTYPA
SPHLATKMDGRALDFNKIEQATHILKQRFDVVLLEGAGGLMVPLTTDMLTIDYIAEHQFPIILVSSGRLGSINHTLLSLE
AIKQRGLSLYALAYNLNDESQDTIISKDTAQYLKQYLKHSFPEAKWIDIPKI

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1786995, Length=172, Percent_Identity=31.3953488372093, Blast_Score=98, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004472
- InterPro:   IPR002586 [H]

Pfam domain/function: PF01656 CbiA [H]

EC number: =6.3.3.3 [H]

Molecular weight: Translated: 23950; Mature: 23819

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASVYFISGIDTGIGKTYATGYLAKQWIDQGIKTITQKLIQTGNIEISEDIEKHRDIMQ
CCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHH
TGFLPEDDEKLTMPEIFTYPASPHLATKMDGRALDFNKIEQATHILKQRFDVVLLEGAGG
HCCCCCCCCCCCCCCEEECCCCCCCEECCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCC
LMVPLTTDMLTIDYIAEHQFPIILVSSGRLGSINHTLLSLEAIKQRGLSLYALAYNLNDE
EEEEEECCEEEEEHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCC
SQDTIISKDTAQYLKQYLKHSFPEAKWIDIPKI
CCCCEECHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TASVYFISGIDTGIGKTYATGYLAKQWIDQGIKTITQKLIQTGNIEISEDIEKHRDIMQ
CEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHH
TGFLPEDDEKLTMPEIFTYPASPHLATKMDGRALDFNKIEQATHILKQRFDVVLLEGAGG
HCCCCCCCCCCCCCCEEECCCCCCCEECCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCC
LMVPLTTDMLTIDYIAEHQFPIILVSSGRLGSINHTLLSLEAIKQRGLSLYALAYNLNDE
EEEEEECCEEEEEHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCC
SQDTIISKDTAQYLKQYLKHSFPEAKWIDIPKI
CCCCEECHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]