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The map label for this gene is bioA [H]

Identifier: 50084068

GI number: 50084068

Start: 843134

End: 844462

Strand: Direct

Name: bioA [H]

Synonym: ACIAD0856

Alternate gene names: 50084068

Gene position: 843134-844462 (Clockwise)

Preceding gene: 50084067

Following gene: 50084069

Centisome position: 23.43

GC content: 42.29

Gene sequence:

>1329_bases
GTGCTCTATTCAATATTTATAGCGCATCAGGAGTCATGGATGGTGGATCAATTTGATTTGGAGCATATCTGGCATCCTTA
TACTTCCATGATTAATCCATTACCAACCTTTAAAGTGAAACGTGCTTCTGGTGTGATGATTGAGCTGGATGATGGTCGTC
AATTAATTGACGGTATGTCATCGTGGTGGTGTGCGATTCATGGTTATAATCATCCAGCATTAAATCAGGCTGTAAAAGAC
CAACTTGAGCAGATGTCTCACATCATGTTTGGGGGGTTTACCCATGATCCTGCGATTGAACTTGCCAAAATATTACTAAA
GATTACGCCGCCGAGTTTGGATAAAATCTTTTATGCAGATTCTGGTTCTGTTGCAGTAGAAGTTGCGTTAAAAATGGCAG
TACAGTATTGGACTGCACAAGGGCAACATCAAAAAACCAATTTCATTACTACCCGTTCTGGATACCATGGCGATACATGG
AATGCGATGTCTGTCTGCGATCCAGTTACAGGTATGCATCAAATTTTTGGTGCAAGTTTGCCAAATCGTGTGTTTGTATC
GGCACCTAAAAGCCGTTTTGATGGAGAATGGGACTCATCTGAAATGGATGAGGTCGAGCAGTTGATCCAGCAGCATACGC
AATTGGCAGCGTTCATTATCGAGCCGATTGTTCAGGGGGCTGGTGGCATGCGGTTTTATCATCCTGAATACTTACGTCAG
TTAAAACAGCTTTGTGAAAAATATCAGTTACTTCTTATTTTTGATGAAATCGCAACAGGTTTTGGTCGTACAGGGAAGTT
ATTTGCGTGGGAGCATGCACAGGTTGAACCTGACATCATGTGTATTGGCAAAGGTTTGACAGGTGGATACATGACTTTAT
CGGCAACTCTAACCAGTCAGCATGTTGCCGAAGTGATTAGTCGTGGAGAGGCTGGCGTCTTTATGCATGGCCCTACTTTT
ATGGGTAATCCTCTGGCCTGTGCTGTGGCATTAAAGAGTACCCAACTTTTGGTTGAGCAAGATTGGCAGTCTAATATCAG
ACGTATTGAAAATAGATTACAGCCGCTCTATCAATTGGCTGCACTAGCGCATGTTGCAGATATAAGAACTTTAGGTGCAA
TTGGTGTGGTAGAACTCAAGCACAATGTTGATATGAAGACCCTCCAGCAACAATTTGTCGAGTTAGGAATCTGGATCAGA
CCGTTTGGTAAATTGGTTTATGTAATGCCACCCTATATCATTTCCAATGATGAACTTGATGAATTAATCCAAAAAATGAT
GAACGTGGTTCGTCAACTTTCTTGTGAGGCGCAGCATGGCATTGCTTGA

Upstream 100 bases:

>100_bases
AACAAGTAGTGAAATTGAAAAATTTATTGTTTCATCAGTATAAGCTTTAGTCGAAATGATGCATATCGCAATTTATTTAC
ATTCCTTGCAAGTTTAACAT

Downstream 100 bases:

>100_bases
TCATTATGCACAACAGCTTGATCAATTAAAACAATCTGGAAATTTACGCCAGTTTGTTGCGAATCAACAACAAGGTGCGA
CCATCCAGATCACAGGCCAG

Product: adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MLYSIFIAHQESWMVDQFDLEHIWHPYTSMINPLPTFKVKRASGVMIELDDGRQLIDGMSSWWCAIHGYNHPALNQAVKD
QLEQMSHIMFGGFTHDPAIELAKILLKITPPSLDKIFYADSGSVAVEVALKMAVQYWTAQGQHQKTNFITTRSGYHGDTW
NAMSVCDPVTGMHQIFGASLPNRVFVSAPKSRFDGEWDSSEMDEVEQLIQQHTQLAAFIIEPIVQGAGGMRFYHPEYLRQ
LKQLCEKYQLLLIFDEIATGFGRTGKLFAWEHAQVEPDIMCIGKGLTGGYMTLSATLTSQHVAEVISRGEAGVFMHGPTF
MGNPLACAVALKSTQLLVEQDWQSNIRRIENRLQPLYQLAALAHVADIRTLGAIGVVELKHNVDMKTLQQQFVELGIWIR
PFGKLVYVMPPYIISNDELDELIQKMMNVVRQLSCEAQHGIA

Sequences:

>Translated_442_residues
MLYSIFIAHQESWMVDQFDLEHIWHPYTSMINPLPTFKVKRASGVMIELDDGRQLIDGMSSWWCAIHGYNHPALNQAVKD
QLEQMSHIMFGGFTHDPAIELAKILLKITPPSLDKIFYADSGSVAVEVALKMAVQYWTAQGQHQKTNFITTRSGYHGDTW
NAMSVCDPVTGMHQIFGASLPNRVFVSAPKSRFDGEWDSSEMDEVEQLIQQHTQLAAFIIEPIVQGAGGMRFYHPEYLRQ
LKQLCEKYQLLLIFDEIATGFGRTGKLFAWEHAQVEPDIMCIGKGLTGGYMTLSATLTSQHVAEVISRGEAGVFMHGPTF
MGNPLACAVALKSTQLLVEQDWQSNIRRIENRLQPLYQLAALAHVADIRTLGAIGVVELKHNVDMKTLQQQFVELGIWIR
PFGKLVYVMPPYIISNDELDELIQKMMNVVRQLSCEAQHGIA
>Mature_442_residues
MLYSIFIAHQESWMVDQFDLEHIWHPYTSMINPLPTFKVKRASGVMIELDDGRQLIDGMSSWWCAIHGYNHPALNQAVKD
QLEQMSHIMFGGFTHDPAIELAKILLKITPPSLDKIFYADSGSVAVEVALKMAVQYWTAQGQHQKTNFITTRSGYHGDTW
NAMSVCDPVTGMHQIFGASLPNRVFVSAPKSRFDGEWDSSEMDEVEQLIQQHTQLAAFIIEPIVQGAGGMRFYHPEYLRQ
LKQLCEKYQLLLIFDEIATGFGRTGKLFAWEHAQVEPDIMCIGKGLTGGYMTLSATLTSQHVAEVISRGEAGVFMHGPTF
MGNPLACAVALKSTQLLVEQDWQSNIRRIENRLQPLYQLAALAHVADIRTLGAIGVVELKHNVDMKTLQQQFVELGIWIR
PFGKLVYVMPPYIISNDELDELIQKMMNVVRQLSCEAQHGIA

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0161

COG function: function code H; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=417, Percent_Identity=28.537170263789, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI284507298, Length=311, Percent_Identity=29.5819935691318, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI37574042, Length=353, Percent_Identity=28.0453257790368, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI13994255, Length=431, Percent_Identity=24.8259860788863, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI226442709, Length=323, Percent_Identity=28.4829721362229, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI226442705, Length=354, Percent_Identity=27.9661016949153, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI24119277, Length=437, Percent_Identity=24.4851258581236, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1786991, Length=416, Percent_Identity=59.375, Blast_Score=504, Evalue=1e-144,
Organism=Escherichia coli, GI145693181, Length=382, Percent_Identity=30.8900523560209, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI1789759, Length=389, Percent_Identity=30.5912596401028, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1788044, Length=394, Percent_Identity=29.6954314720812, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1789016, Length=406, Percent_Identity=25.615763546798, Blast_Score=135, Evalue=6e-33,
Organism=Escherichia coli, GI1787560, Length=411, Percent_Identity=26.0340632603406, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1786349, Length=322, Percent_Identity=26.7080745341615, Blast_Score=102, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI25144271, Length=424, Percent_Identity=26.8867924528302, Blast_Score=141, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI25144274, Length=236, Percent_Identity=30.9322033898305, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI32564660, Length=425, Percent_Identity=26.3529411764706, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI71992977, Length=428, Percent_Identity=23.8317757009346, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324386, Length=457, Percent_Identity=44.4201312910284, Blast_Score=403, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6323470, Length=417, Percent_Identity=26.1390887290168, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6324432, Length=382, Percent_Identity=25.9162303664921, Blast_Score=112, Evalue=8e-26,
Organism=Drosophila melanogaster, GI21357415, Length=397, Percent_Identity=27.7078085642317, Blast_Score=148, Evalue=9e-36,
Organism=Drosophila melanogaster, GI161085790, Length=380, Percent_Identity=26.5789473684211, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI28574759, Length=380, Percent_Identity=26.5789473684211, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI21356575, Length=424, Percent_Identity=23.5849056603774, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI281366494, Length=336, Percent_Identity=26.4880952380952, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24667139, Length=336, Percent_Identity=26.4880952380952, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24667143, Length=336, Percent_Identity=26.4880952380952, Blast_Score=89, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR005814
- InterPro:   IPR005815
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR007197 [H]

Pfam domain/function: PF00202 Aminotran_3; PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 49823; Mature: 49823

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYSIFIAHQESWMVDQFDLEHIWHPYTSMINPLPTFKVKRASGVMIELDDGRQLIDGMS
CEEEEEEEECCCCCCCHHCHHHHHCCHHHHCCCCCCEEEECCCCEEEEECCCHHHHHCHH
SWWCAIHGYNHPALNQAVKDQLEQMSHIMFGGFTHDPAIELAKILLKITPPSLDKIFYAD
HHEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEEC
SGSVAVEVALKMAVQYWTAQGQHQKTNFITTRSGYHGDTWNAMSVCDPVTGMHQIFGASL
CCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCHHHHHCCHHHHHHHHCCCC
PNRVFVSAPKSRFDGEWDSSEMDEVEQLIQQHTQLAAFIIEPIVQGAGGMRFYHPEYLRQ
CCCEEEECCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECHHHHHH
LKQLCEKYQLLLIFDEIATGFGRTGKLFAWEHAQVEPDIMCIGKGLTGGYMTLSATLTSQ
HHHHHHHHHHHEEEHHHHHCCCCCCCEEEEECCCCCCCEEEEECCCCCCEEEEEEHHHHH
HVAEVISRGEAGVFMHGPTFMGNPLACAVALKSTQLLVEQDWQSNIRRIENRLQPLYQLA
HHHHHHHCCCCCEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALAHVADIRTLGAIGVVELKHNVDMKTLQQQFVELGIWIRPFGKLVYVMPPYIISNDELD
HHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHCCCEECCCCEEEECCCEEECCCCHH
ELIQKMMNVVRQLSCEAQHGIA
HHHHHHHHHHHHHCCCHHCCCC
>Mature Secondary Structure
MLYSIFIAHQESWMVDQFDLEHIWHPYTSMINPLPTFKVKRASGVMIELDDGRQLIDGMS
CEEEEEEEECCCCCCCHHCHHHHHCCHHHHCCCCCCEEEECCCCEEEEECCCHHHHHCHH
SWWCAIHGYNHPALNQAVKDQLEQMSHIMFGGFTHDPAIELAKILLKITPPSLDKIFYAD
HHEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEEC
SGSVAVEVALKMAVQYWTAQGQHQKTNFITTRSGYHGDTWNAMSVCDPVTGMHQIFGASL
CCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCHHHHHCCHHHHHHHHCCCC
PNRVFVSAPKSRFDGEWDSSEMDEVEQLIQQHTQLAAFIIEPIVQGAGGMRFYHPEYLRQ
CCCEEEECCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECHHHHHH
LKQLCEKYQLLLIFDEIATGFGRTGKLFAWEHAQVEPDIMCIGKGLTGGYMTLSATLTSQ
HHHHHHHHHHHEEEHHHHHCCCCCCCEEEEECCCCCCCEEEEECCCCCCEEEEEEHHHHH
HVAEVISRGEAGVFMHGPTFMGNPLACAVALKSTQLLVEQDWQSNIRRIENRLQPLYQLA
HHHHHHHCCCCCEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALAHVADIRTLGAIGVVELKHNVDMKTLQQQFVELGIWIRPFGKLVYVMPPYIISNDELD
HHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHCCCEECCCCEEEECCCEEECCCCHH
ELIQKMMNVVRQLSCEAQHGIA
HHHHHHHHHHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA