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The map label for this gene is bioH [H]

Identifier: 50084067

GI number: 50084067

Start: 842318

End: 843076

Strand: Direct

Name: bioH [H]

Synonym: ACIAD0855

Alternate gene names: 50084067

Gene position: 842318-843076 (Clockwise)

Preceding gene: 50084065

Following gene: 50084068

Centisome position: 23.41

GC content: 37.55

Gene sequence:

>759_bases
TTGCAAGATAATTTTGAAAATTTGAAAAAGAGTAGATCAATGGCTCGGGTTTTGCTGATTACAGGGTGGGGCGGAGGAAC
AGGTGTGTTAACTCCCTTACAAAACAGTTTGCAACAATTGGGGCACGAAGTTGAATTACTCAATATATTTAATGCACTTG
ATCCCGATATATTACAACAACAAACTGATTATGCACTGAACTTTGATGTTCTGATGGGATGGTCATTGGGGGGAGAGTTG
GCAACTGTATTGGCCCATGAGATTTTTAAGCGCACTGGTCAAACATTACCGCTGGTCACTTTAGCAACCAATCCGAGTTT
TGTAGTACGAGAAAATTGGTCAACTGCTATGCCAGAAACAACGTTTAATGCATTTCAGCAGGGCTTTCAGAATGATCCTG
CCAGTACGCTAAAACGATTTGGTTTACTGGTTACCAAAGGGACGTCAACTGCAAAATCAGATTGGATATCAATGCAGGCT
TTATTAAAAGCACAGCCATTAGCTTTGCTCAGGCAAGGCTTAATTATGTTGGAAAAATTAAATTTAGTTGAAATCATTAA
AAATTATGGAGGATTTCAGCTACATATATTTGCCGAGCATGATGCATTAGTTCCTTACCAAGTTGTGCAAAATATGCATC
TTATTGCGACAAAAAAAACTAAAATAATTGAGCTAGCGAGTGCAGCGCACAGTTTTCCATTTACAAAGTTAATGGAAACA
AGTAGTGAAATTGAAAAATTTATTGTTTCATCAGTATAA

Upstream 100 bases:

>100_bases
CTTTTTATTTTCACGGGTTAAATACTATCAGTTTTAATGATCGAAAGCTGTAGTATTGATGTGATTATGCCATAGCTATA
GATGTTAACTCTTCTATACT

Downstream 100 bases:

>100_bases
GCTTTAGTCGAAATGATGCATATCGCAATTTATTTACATTCCTTGCAAGTTTAACATGTGCTCTATTCAATATTTATAGC
GCATCAGGAGTCATGGATGG

Product: hydrolase, biotin biosynthesis (BioH)

Products: NA

Alternate protein names: Biotin synthesis protein BioH [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MQDNFENLKKSRSMARVLLITGWGGGTGVLTPLQNSLQQLGHEVELLNIFNALDPDILQQQTDYALNFDVLMGWSLGGEL
ATVLAHEIFKRTGQTLPLVTLATNPSFVVRENWSTAMPETTFNAFQQGFQNDPASTLKRFGLLVTKGTSTAKSDWISMQA
LLKAQPLALLRQGLIMLEKLNLVEIIKNYGGFQLHIFAEHDALVPYQVVQNMHLIATKKTKIIELASAAHSFPFTKLMET
SSEIEKFIVSSV

Sequences:

>Translated_252_residues
MQDNFENLKKSRSMARVLLITGWGGGTGVLTPLQNSLQQLGHEVELLNIFNALDPDILQQQTDYALNFDVLMGWSLGGEL
ATVLAHEIFKRTGQTLPLVTLATNPSFVVRENWSTAMPETTFNAFQQGFQNDPASTLKRFGLLVTKGTSTAKSDWISMQA
LLKAQPLALLRQGLIMLEKLNLVEIIKNYGGFQLHIFAEHDALVPYQVVQNMHLIATKKTKIIELASAAHSFPFTKLMET
SSEIEKFIVSSV
>Mature_252_residues
MQDNFENLKKSRSMARVLLITGWGGGTGVLTPLQNSLQQLGHEVELLNIFNALDPDILQQQTDYALNFDVLMGWSLGGEL
ATVLAHEIFKRTGQTLPLVTLATNPSFVVRENWSTAMPETTFNAFQQGFQNDPASTLKRFGLLVTKGTSTAKSDWISMQA
LLKAQPLALLRQGLIMLEKLNLVEIIKNYGGFQLHIFAEHDALVPYQVVQNMHLIATKKTKIIELASAAHSFPFTKLMET
SSEIEKFIVSSV

Specific function: Shows carboxylesterase activity with a preference for short chain fatty acid esters (acyl chain length of up to 6 carbons). Also displays a weak thioesterase activity. Can form a complex with CoA, and may be involved in the condensation of CoA and pimelic

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family [H]

Homologues:

Organism=Escherichia coli, GI1789817, Length=181, Percent_Identity=32.0441988950276, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010076 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 28005; Mature: 28005

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDNFENLKKSRSMARVLLITGWGGGTGVLTPLQNSLQQLGHEVELLNIFNALDPDILQQ
CCCCHHHHHHHHHHEEEEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHH
QTDYALNFDVLMGWSLGGELATVLAHEIFKRTGQTLPLVTLATNPSFVVRENWSTAMPET
HCCCEEEEHEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEECCCCCCCCHH
TFNAFQQGFQNDPASTLKRFGLLVTKGTSTAKSDWISMQALLKAQPLALLRQGLIMLEKL
HHHHHHHHCCCCHHHHHHHHCEEEECCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
NLVEIIKNYGGFQLHIFAEHDALVPYQVVQNMHLIATKKTKIIELASAAHSFPFTKLMET
HHHHHHHCCCCEEEEEEECCCCCCHHHHHCCCEEEEECCHHHHHHHHHHHCCCHHHHHHH
SSEIEKFIVSSV
HHHHHHHHHHCC
>Mature Secondary Structure
MQDNFENLKKSRSMARVLLITGWGGGTGVLTPLQNSLQQLGHEVELLNIFNALDPDILQQ
CCCCHHHHHHHHHHEEEEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHH
QTDYALNFDVLMGWSLGGELATVLAHEIFKRTGQTLPLVTLATNPSFVVRENWSTAMPET
HCCCEEEEHEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEECCCCCCCCHH
TFNAFQQGFQNDPASTLKRFGLLVTKGTSTAKSDWISMQALLKAQPLALLRQGLIMLEKL
HHHHHHHHCCCCHHHHHHHHCEEEECCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
NLVEIIKNYGGFQLHIFAEHDALVPYQVVQNMHLIATKKTKIIELASAAHSFPFTKLMET
HHHHHHHCCCCEEEEEEECCCCCCHHHHHCCCEEEEECCHHHHHHHHHHHCCCHHHHHHH
SSEIEKFIVSSV
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA