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The map label for this gene is gatA

Identifier: 50084037

GI number: 50084037

Start: 812576

End: 814054

Strand: Reverse

Name: gatA

Synonym: ACIAD0823

Alternate gene names: 50084037

Gene position: 814054-812576 (Counterclockwise)

Preceding gene: 50084038

Following gene: 50084036

Centisome position: 22.62

GC content: 47.73

Gene sequence:

>1479_bases
ATGACCGATTTACACCGCTTATCTGTTCGTGAGCTCGCCGAGGGTCTAGGCCAAGCTCAATTTTCTTCCCGCGAACTCAC
GCAACATTATCTGAACCGCATCGATAAAATCGACGCTCAGGTAAAAAGTTATGTCACTGTGACCCACGAACAGGCACTGG
CACAGGCCGATGCTGCCGATGCATTGCGTCAGGCTGGCAATGCTGGATTCCTCACTGGCGTTCCATTGGCACATAAAGAT
ATCTTTTGTACCAAAGGCATTAAAACCACTGCTGGTTCTAAAATGCTCGACAACTTTATCTCGCCTTACAATGCAACTGT
AGTTGAAAAAGCCAATGCAGCAGGTTTAGTGACCTTGGGCAAACTCAATATGGATGAGTTTGCCATGGGCTCGACCTCGG
AAAGCTCGTATTTTGGTGCAACGTGTAACCCATGGGCACTCGACCGTGTACCAGGCGGTTCATCGGGTGGTTCTGCTGCT
GCTGTAGCAGCAGATCTTGCTCCGTTTGCCACAGGGACAGACACGGGCGGCTCTATTCGTCAGCCGGCGTCGTTTTGTGG
TTTAACAGGTTTAAAGCCAACCTACGGCCGTGTATCACGCTTTGGTATGATCGCCTATGCATCATCGCTGGATCAGGGCG
GCCCAATGGCACGCTCTGCCGAAGATTGCGCTTACCTGATGAATGTCATGGCAGGTCATGATGCCAAAGATTCAACGTCT
GTCAAAAAAGACGTCGATGACTATGTAGCGAACTTAAATGCAACAGCACTAAAAGGCCTGCGTATTGGTATTCCAAAGCA
ATACTTCAATGTGGCAGGCTTAGATGCCGAGGTGAAAGCGCGCGTAGAAGAATCTCTAAAAAAACTTGAAGAGATGGGCG
CGGTACTGGTCGAGATTGATCTCAGTATGACCGAAAGCTATGTACCCACTTATTACCTGATTGCACCTGCTGAAGCCTCA
TCGAATCTATCGCGTTATGACGGTGTGCGTTATGGCTATCGCTGTGAAAATCCAGTCGATTTGATGGATTTATACAAGCG
TTCACGCTCAGAAGGCTTTGGTGCAGAAGTACAACGCCGTATCTTGATTGGTACTTATGCCCTTTCGGCTGGCTATTATG
ATGCCTATTATGTGAAAGCTCAGAAAGTGCGCCGCTTAATTCAGCAAGATTTCTTAAAAGCATTTGAAAATGTAGATGTA
ATTGCTGCACCGTCTGCACCAACTACAGCTTATAAAATTGGTGCCGATCTCACACCAGTTGAAATGTATTTGGGCGATAT
TTATACCCTTGCAGTGAACCTTGCAGGCTTACCAGCCATCAATGCACCTGTGGGCCTCGACAGCAATAATTTGCCTGTCG
GTTTGCAACTGATTGGTAATTACTGGTCTGAGTCGCAATTGCTTTCGATTGTGCATCAGTACCAACAAGACACCACCTTC
CACACACAACGTGCGGCAATTGCTGAGGAGAATGCCTAA

Upstream 100 bases:

>100_bases
CCAGCAACCGAAGCTGGACTGTATCTGGTTCCTCGCGTAATCGAATAATACTCTCGCTACTTAAATTGCTTACAATTTTG
AACGTAAAGAATTTTTACTT

Downstream 100 bases:

>100_bases
TGAATAATCCTTCAAAAGCAGCTCAAAAAAAGCCAAAGTCGAACCTGATTGATGGTTGGGAAGTGGTGATCGGGATTGAG
ATTCATACCCAGCTTGCAAC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MTDLHRLSVRELAEGLGQAQFSSRELTQHYLNRIDKIDAQVKSYVTVTHEQALAQADAADALRQAGNAGFLTGVPLAHKD
IFCTKGIKTTAGSKMLDNFISPYNATVVEKANAAGLVTLGKLNMDEFAMGSTSESSYFGATCNPWALDRVPGGSSGGSAA
AVAADLAPFATGTDTGGSIRQPASFCGLTGLKPTYGRVSRFGMIAYASSLDQGGPMARSAEDCAYLMNVMAGHDAKDSTS
VKKDVDDYVANLNATALKGLRIGIPKQYFNVAGLDAEVKARVEESLKKLEEMGAVLVEIDLSMTESYVPTYYLIAPAEAS
SNLSRYDGVRYGYRCENPVDLMDLYKRSRSEGFGAEVQRRILIGTYALSAGYYDAYYVKAQKVRRLIQQDFLKAFENVDV
IAAPSAPTTAYKIGADLTPVEMYLGDIYTLAVNLAGLPAINAPVGLDSNNLPVGLQLIGNYWSESQLLSIVHQYQQDTTF
HTQRAAIAEENA

Sequences:

>Translated_492_residues
MTDLHRLSVRELAEGLGQAQFSSRELTQHYLNRIDKIDAQVKSYVTVTHEQALAQADAADALRQAGNAGFLTGVPLAHKD
IFCTKGIKTTAGSKMLDNFISPYNATVVEKANAAGLVTLGKLNMDEFAMGSTSESSYFGATCNPWALDRVPGGSSGGSAA
AVAADLAPFATGTDTGGSIRQPASFCGLTGLKPTYGRVSRFGMIAYASSLDQGGPMARSAEDCAYLMNVMAGHDAKDSTS
VKKDVDDYVANLNATALKGLRIGIPKQYFNVAGLDAEVKARVEESLKKLEEMGAVLVEIDLSMTESYVPTYYLIAPAEAS
SNLSRYDGVRYGYRCENPVDLMDLYKRSRSEGFGAEVQRRILIGTYALSAGYYDAYYVKAQKVRRLIQQDFLKAFENVDV
IAAPSAPTTAYKIGADLTPVEMYLGDIYTLAVNLAGLPAINAPVGLDSNNLPVGLQLIGNYWSESQLLSIVHQYQQDTTF
HTQRAAIAEENA
>Mature_491_residues
TDLHRLSVRELAEGLGQAQFSSRELTQHYLNRIDKIDAQVKSYVTVTHEQALAQADAADALRQAGNAGFLTGVPLAHKDI
FCTKGIKTTAGSKMLDNFISPYNATVVEKANAAGLVTLGKLNMDEFAMGSTSESSYFGATCNPWALDRVPGGSSGGSAAA
VAADLAPFATGTDTGGSIRQPASFCGLTGLKPTYGRVSRFGMIAYASSLDQGGPMARSAEDCAYLMNVMAGHDAKDSTSV
KKDVDDYVANLNATALKGLRIGIPKQYFNVAGLDAEVKARVEESLKKLEEMGAVLVEIDLSMTESYVPTYYLIAPAEASS
NLSRYDGVRYGYRCENPVDLMDLYKRSRSEGFGAEVQRRILIGTYALSAGYYDAYYVKAQKVRRLIQQDFLKAFENVDVI
AAPSAPTTAYKIGADLTPVEMYLGDIYTLAVNLAGLPAINAPVGLDSNNLPVGLQLIGNYWSESQLLSIVHQYQQDTTFH
TQRAAIAEENA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=499, Percent_Identity=37.875751503006, Blast_Score=324, Evalue=1e-88,
Organism=Homo sapiens, GI195972892, Length=501, Percent_Identity=26.7465069860279, Blast_Score=142, Evalue=6e-34,
Organism=Homo sapiens, GI166795287, Length=170, Percent_Identity=37.0588235294118, Blast_Score=85, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17543272, Length=441, Percent_Identity=37.8684807256236, Blast_Score=276, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI17537465, Length=496, Percent_Identity=25.2016129032258, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17556264, Length=230, Percent_Identity=29.5652173913043, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71990152, Length=242, Percent_Identity=32.2314049586777, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17538252, Length=239, Percent_Identity=29.2887029288703, Blast_Score=82, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17556276, Length=332, Percent_Identity=27.4096385542169, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17556278, Length=332, Percent_Identity=27.4096385542169, Blast_Score=79, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6323950, Length=433, Percent_Identity=36.4896073903002, Blast_Score=240, Evalue=4e-64,
Organism=Saccharomyces cerevisiae, GI6319685, Length=420, Percent_Identity=30, Blast_Score=114, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6320448, Length=232, Percent_Identity=29.7413793103448, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648113, Length=502, Percent_Identity=40.0398406374502, Blast_Score=306, Evalue=3e-83,
Organism=Drosophila melanogaster, GI24644968, Length=506, Percent_Identity=26.4822134387352, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24652985, Length=492, Percent_Identity=26.4227642276423, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI19922090, Length=492, Percent_Identity=26.4227642276423, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24652981, Length=492, Percent_Identity=26.4227642276423, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24652983, Length=492, Percent_Identity=26.4227642276423, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI21356731, Length=499, Percent_Identity=25.8517034068136, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI161078093, Length=495, Percent_Identity=26.0606060606061, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI45550774, Length=253, Percent_Identity=29.2490118577075, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24648435, Length=253, Percent_Identity=29.2490118577075, Blast_Score=96, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24648437, Length=253, Percent_Identity=29.2490118577075, Blast_Score=96, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24648441, Length=185, Percent_Identity=32.972972972973, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24648439, Length=185, Percent_Identity=32.972972972973, Blast_Score=82, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_ACIAD (Q6FDY3)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_045547.1
- ProteinModelPortal:   Q6FDY3
- SMR:   Q6FDY3
- STRING:   Q6FDY3
- GeneID:   2878485
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD0823
- NMPDR:   fig|62977.3.peg.737
- eggNOG:   COG0154
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- PhylomeDB:   Q6FDY3
- ProtClustDB:   PRK00012
- BioCyc:   ASP62977:ACIAD0823-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 52978; Mature: 52847

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 79-79 ACT_SITE 154-154 ACT_SITE 178-178

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLHRLSVRELAEGLGQAQFSSRELTQHYLNRIDKIDAQVKSYVTVTHEQALAQADAAD
CCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHH
ALRQAGNAGFLTGVPLAHKDIFCTKGIKTTAGSKMLDNFISPYNATVVEKANAAGLVTLG
HHHHCCCCCCEECCCCCCCCEEEECCCCCCCCHHHHHHHCCCCCCEEEECCCCCCEEEEE
KLNMDEFAMGSTSESSYFGATCNPWALDRVPGGSSGGSAAAVAADLAPFATGTDTGGSIR
CCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCC
QPASFCGLTGLKPTYGRVSRFGMIAYASSLDQGGPMARSAEDCAYLMNVMAGHDAKDSTS
CCHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCH
VKKDVDDYVANLNATALKGLRIGIPKQYFNVAGLDAEVKARVEESLKKLEEMGAVLVEID
HHHHHHHHHHCCCHHHHCCEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEE
LSMTESYVPTYYLIAPAEASSNLSRYDGVRYGYRCENPVDLMDLYKRSRSEGFGAEVQRR
HHHHHCCCCEEEEEEECCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHH
ILIGTYALSAGYYDAYYVKAQKVRRLIQQDFLKAFENVDVIAAPSAPTTAYKIGADLTPV
HEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCEEECCCCCCHH
EMYLGDIYTLAVNLAGLPAINAPVGLDSNNLPVGLQLIGNYWSESQLLSIVHQYQQDTTF
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCH
HTQRAAIAEENA
HHHHHHHHCCCC
>Mature Secondary Structure 
TDLHRLSVRELAEGLGQAQFSSRELTQHYLNRIDKIDAQVKSYVTVTHEQALAQADAAD
CCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHH
ALRQAGNAGFLTGVPLAHKDIFCTKGIKTTAGSKMLDNFISPYNATVVEKANAAGLVTLG
HHHHCCCCCCEECCCCCCCCEEEECCCCCCCCHHHHHHHCCCCCCEEEECCCCCCEEEEE
KLNMDEFAMGSTSESSYFGATCNPWALDRVPGGSSGGSAAAVAADLAPFATGTDTGGSIR
CCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCC
QPASFCGLTGLKPTYGRVSRFGMIAYASSLDQGGPMARSAEDCAYLMNVMAGHDAKDSTS
CCHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCH
VKKDVDDYVANLNATALKGLRIGIPKQYFNVAGLDAEVKARVEESLKKLEEMGAVLVEID
HHHHHHHHHHCCCHHHHCCEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEE
LSMTESYVPTYYLIAPAEASSNLSRYDGVRYGYRCENPVDLMDLYKRSRSEGFGAEVQRR
HHHHHCCCCEEEEEEECCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHH
ILIGTYALSAGYYDAYYVKAQKVRRLIQQDFLKAFENVDVIAAPSAPTTAYKIGADLTPV
HEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCEEECCCCCCHH
EMYLGDIYTLAVNLAGLPAINAPVGLDSNNLPVGLQLIGNYWSESQLLSIVHQYQQDTTF
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCH
HTQRAAIAEENA
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA