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The map label for this gene is gatB

Identifier: 50084036

GI number: 50084036

Start: 811089

End: 812576

Strand: Reverse

Name: gatB

Synonym: ACIAD0822

Alternate gene names: 50084036

Gene position: 812576-811089 (Counterclockwise)

Preceding gene: 50084037

Following gene: 50084028

Centisome position: 22.58

GC content: 45.56

Gene sequence:

>1488_bases
ATGAATAATCCTTCAAAAGCAGCTCAAAAAAAGCCAAAGTCGAACCTGATTGATGGTTGGGAAGTGGTGATCGGGATTGA
GATTCATACCCAGCTTGCAACCCAATCTAAAATTTTCTCGGGTTCTTCTACCGAATTTGGACAAGATCCGAATACGCAAG
CAAGCTTGGTCGATTTGGCCATGCCAGGTGTATTGCCAGTATTAAATGAAGAAGTGGTGAATCTTGCGATCCGCTTTGGC
TTGGGCATCGATGCATATATCGATCAGGCTTCTGTATTTGCACGTAAAAACTATTTCTACCCAGATTCGCCAAAAGGCTA
TCAGATCAGCCAGATGGACAATCCGATTGTGGGCTTGGGTCATATCGACATCCAGCTTGAAGATGGCACCGTCAAACGCA
TCGGTGTAACGCGTGCGCATTTGGAAGAAGATGCAGGTAAATCGATTCATGACCAGTTTGAAGGTCAGTCGGGTATCGAT
TTAAACCGCGCCGGTACGCCATTGCTCGAAATTGTATCTGAACCAGATATGCGTTCGGTCGAAGAAGCGGTTGCGTATAT
CAAATCGATACATACGCTCGTGCGCTGGTTAGGGATTTCTGACGGTAACATGGCAGAAGGCTCATTCCGTGCCGACTGTA
ACGTGTCATTGCGCCGTCCGGGCGATGCATTTGGTACCCGCTGTGAGCTGAAAAACCTTAACTCGTTCCGTTTTATCGAG
CAAGCGATCAATGTCGAAATTGAGCGCCAGATGGAAATTTTGGAATGGGGCGGTACGATTGATCAGGAAACCCGTTTGTT
TGATCCAAACAAAATGGAAACCCGCTCAATGCGTTCTAAAGAAGAAGCGAACGACTATCGCTACTTCCCAGATCCAGACT
TGTTGCCTGTGATCATTGCAGATGAGCAAATTAAAGCAATTAAAGCGACTATGCCGGAGCTACCTGCTGCTCGCCGTGAA
CGATTTATCGCTGAATTTGCAATTACCGAATACGATGCGCATGTACTTACCTTGTCTCGTGAAATGGCCGATTTTTATGA
AGCGGTCGTGGCAGCGGCTGGCGGTGCAGCACACGGTAAAATTGCTGCAAACTGGGTGATGGGTGAATTCTCTGGGGCCT
TAAATAAAGCTGGCCTAGAGCTTGCAGATTCGCCTGTGTCTGCTGAGCAATTGGGTGGCATGATTGCACGTATTGTGGAT
AACACCATCAGCGGTAAAATTGCAAAACAAGTCTTTGGCTTTATGTGGGATGAAGGCAAATCTGCCGATACCATTATTCA
GGAAAAAAGTTTAAAGCAAGAAACTGACACAGGTGCGATTGAAGCCATTATTAAAGACGTACTTGCAGCCAATGAAAAAA
TGGTTGAGGAGTATAAGTCTGGTAAAGAGAAAGCTTTTAATGGCCTTGTAGGTCAAGTGATGAAAGCTTCAAAAGGCAAA
GCCAACCCTGCTCAAGTGAATGAATTGATGAAGAAATTGATTGGTTAA

Upstream 100 bases:

>100_bases
ATTACTGGTCTGAGTCGCAATTGCTTTCGATTGTGCATCAGTACCAACAAGACACCACCTTCCACACACAACGTGCGGCA
ATTGCTGAGGAGAATGCCTA

Downstream 100 bases:

>100_bases
GCTAATCTAGTTTCAAAGAAAACCCGCTTTAGAGCGGGTTTTCTTATTCAGAGGTTAATAAAAACTCTCTTCTTCGACTA
AAACATTTTTAACATAAAGT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MNNPSKAAQKKPKSNLIDGWEVVIGIEIHTQLATQSKIFSGSSTEFGQDPNTQASLVDLAMPGVLPVLNEEVVNLAIRFG
LGIDAYIDQASVFARKNYFYPDSPKGYQISQMDNPIVGLGHIDIQLEDGTVKRIGVTRAHLEEDAGKSIHDQFEGQSGID
LNRAGTPLLEIVSEPDMRSVEEAVAYIKSIHTLVRWLGISDGNMAEGSFRADCNVSLRRPGDAFGTRCELKNLNSFRFIE
QAINVEIERQMEILEWGGTIDQETRLFDPNKMETRSMRSKEEANDYRYFPDPDLLPVIIADEQIKAIKATMPELPAARRE
RFIAEFAITEYDAHVLTLSREMADFYEAVVAAAGGAAHGKIAANWVMGEFSGALNKAGLELADSPVSAEQLGGMIARIVD
NTISGKIAKQVFGFMWDEGKSADTIIQEKSLKQETDTGAIEAIIKDVLAANEKMVEEYKSGKEKAFNGLVGQVMKASKGK
ANPAQVNELMKKLIG

Sequences:

>Translated_495_residues
MNNPSKAAQKKPKSNLIDGWEVVIGIEIHTQLATQSKIFSGSSTEFGQDPNTQASLVDLAMPGVLPVLNEEVVNLAIRFG
LGIDAYIDQASVFARKNYFYPDSPKGYQISQMDNPIVGLGHIDIQLEDGTVKRIGVTRAHLEEDAGKSIHDQFEGQSGID
LNRAGTPLLEIVSEPDMRSVEEAVAYIKSIHTLVRWLGISDGNMAEGSFRADCNVSLRRPGDAFGTRCELKNLNSFRFIE
QAINVEIERQMEILEWGGTIDQETRLFDPNKMETRSMRSKEEANDYRYFPDPDLLPVIIADEQIKAIKATMPELPAARRE
RFIAEFAITEYDAHVLTLSREMADFYEAVVAAAGGAAHGKIAANWVMGEFSGALNKAGLELADSPVSAEQLGGMIARIVD
NTISGKIAKQVFGFMWDEGKSADTIIQEKSLKQETDTGAIEAIIKDVLAANEKMVEEYKSGKEKAFNGLVGQVMKASKGK
ANPAQVNELMKKLIG
>Mature_495_residues
MNNPSKAAQKKPKSNLIDGWEVVIGIEIHTQLATQSKIFSGSSTEFGQDPNTQASLVDLAMPGVLPVLNEEVVNLAIRFG
LGIDAYIDQASVFARKNYFYPDSPKGYQISQMDNPIVGLGHIDIQLEDGTVKRIGVTRAHLEEDAGKSIHDQFEGQSGID
LNRAGTPLLEIVSEPDMRSVEEAVAYIKSIHTLVRWLGISDGNMAEGSFRADCNVSLRRPGDAFGTRCELKNLNSFRFIE
QAINVEIERQMEILEWGGTIDQETRLFDPNKMETRSMRSKEEANDYRYFPDPDLLPVIIADEQIKAIKATMPELPAARRE
RFIAEFAITEYDAHVLTLSREMADFYEAVVAAAGGAAHGKIAANWVMGEFSGALNKAGLELADSPVSAEQLGGMIARIVD
NTISGKIAKQVFGFMWDEGKSADTIIQEKSLKQETDTGAIEAIIKDVLAANEKMVEEYKSGKEKAFNGLVGQVMKASKGK
ANPAQVNELMKKLIG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=511, Percent_Identity=38.747553816047, Blast_Score=357, Evalue=1e-98,
Organism=Caenorhabditis elegans, GI115534094, Length=472, Percent_Identity=30.9322033898305, Blast_Score=205, Evalue=5e-53,
Organism=Saccharomyces cerevisiae, GI6319391, Length=520, Percent_Identity=32.3076923076923, Blast_Score=236, Evalue=7e-63,
Organism=Drosophila melanogaster, GI45550808, Length=490, Percent_Identity=33.0612244897959, Blast_Score=295, Evalue=7e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_ACIAD (Q6FDY4)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_045546.1
- ProteinModelPortal:   Q6FDY4
- SMR:   Q6FDY4
- STRING:   Q6FDY4
- GeneID:   2878486
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD0822
- NMPDR:   fig|62977.3.peg.736
- eggNOG:   COG0064
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- PhylomeDB:   Q6FDY4
- ProtClustDB:   PRK05477
- BioCyc:   ASP62977:ACIAD0822-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 54360; Mature: 54360

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNPSKAAQKKPKSNLIDGWEVVIGIEIHTQLATQSKIFSGSSTEFGQDPNTQASLVDLA
CCCCHHHHHCCCCCCCCCCEEEEEEEEEEHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHH
MPGVLPVLNEEVVNLAIRFGLGIDAYIDQASVFARKNYFYPDSPKGYQISQMDNPIVGLG
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCEEEEE
HIDIQLEDGTVKRIGVTRAHLEEDAGKSIHDQFEGQSGIDLNRAGTPLLEIVSEPDMRSV
EEEEEECCCCEEECCCHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCHHHHHHCCCCHHHH
EEAVAYIKSIHTLVRWLGISDGNMAEGSFRADCNVSLRRPGDAFGTRCELKNLNSFRFIE
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCHHCCEEECCCCCHHHHHH
QAINVEIERQMEILEWGGTIDQETRLFDPNKMETRSMRSKEEANDYRYFPDPDLLPVIIA
HHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEC
DEQIKAIKATMPELPAARRERFIAEFAITEYDAHVLTLSREMADFYEAVVAAAGGAAHGK
CCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
IAANWVMGEFSGALNKAGLELADSPVSAEQLGGMIARIVDNTISGKIAKQVFGFMWDEGK
EEEHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SADTIIQEKSLKQETDTGAIEAIIKDVLAANEKMVEEYKSGKEKAFNGLVGQVMKASKGK
CHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCC
ANPAQVNELMKKLIG
CCHHHHHHHHHHHCC
>Mature Secondary Structure
MNNPSKAAQKKPKSNLIDGWEVVIGIEIHTQLATQSKIFSGSSTEFGQDPNTQASLVDLA
CCCCHHHHHCCCCCCCCCCEEEEEEEEEEHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHH
MPGVLPVLNEEVVNLAIRFGLGIDAYIDQASVFARKNYFYPDSPKGYQISQMDNPIVGLG
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCEEEEE
HIDIQLEDGTVKRIGVTRAHLEEDAGKSIHDQFEGQSGIDLNRAGTPLLEIVSEPDMRSV
EEEEEECCCCEEECCCHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCHHHHHHCCCCHHHH
EEAVAYIKSIHTLVRWLGISDGNMAEGSFRADCNVSLRRPGDAFGTRCELKNLNSFRFIE
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCHHCCEEECCCCCHHHHHH
QAINVEIERQMEILEWGGTIDQETRLFDPNKMETRSMRSKEEANDYRYFPDPDLLPVIIA
HHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEC
DEQIKAIKATMPELPAARRERFIAEFAITEYDAHVLTLSREMADFYEAVVAAAGGAAHGK
CCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
IAANWVMGEFSGALNKAGLELADSPVSAEQLGGMIARIVDNTISGKIAKQVFGFMWDEGK
EEEHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SADTIIQEKSLKQETDTGAIEAIIKDVLAANEKMVEEYKSGKEKAFNGLVGQVMKASKGK
CHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCC
ANPAQVNELMKKLIG
CCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA