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The map label for this gene is ydgK [H]

Identifier: 50084016

GI number: 50084016

Start: 794135

End: 795346

Strand: Direct

Name: ydgK [H]

Synonym: ACIAD0802

Alternate gene names: 50084016

Gene position: 794135-795346 (Clockwise)

Preceding gene: 50084011

Following gene: 50084018

Centisome position: 22.07

GC content: 42.33

Gene sequence:

>1212_bases
ATGTCTGAAAGTAGATTAAATCGTCAATACTCAACTGCATGGATCATGCTCCTTGCACTTTTAACTGCACTCGGTCCATT
ATCTATCGATATGTATCTGCCTGCCTTGCCACAAATGGCAGAGGATTTTGGTGTAAGTACTGCAATGGTAGCAAATACCT
TGCCAGCTTATTTTTTAGGGCTTGCAATTGGTCAATTGGTATACGGCCCGATTAGTGATCGTATTGGTCGTAAAAAGCCT
TTATACGTGGGACTAACACTGTATGTGATCGCCAGTGCATCTTGTGTCCTGGCAGATAATGAATGGTCGCTCATCTTTGC
ACGTATTATTCAGGCATTAGGTGGATGCGTGGGTGTCGTGATTGCAAGAGCAGCGATTCGTGATCGACTCGATGTACAGG
GTTCTGCTCAAGCATTTTCAAGTATGATGATTGTAATGGGAATTGCACCTATTGCAGCACCTGCATTAGGAGCGTTTGTA
CTGGAGTTTTTTCACTGGCAGGCCATTTTCTGGATATTGGCATTGATTGGCTGTATTTCTTTGGTGTGTATTCATTTTTT
CTTTATAGAAACTTTGGTTCCCGAGAAGCGTCTTCGACTCAGTCTCAATCAGGTGTTTACACTCTATATTGCGATCTTTA
AAGATGCGAGCTTTCGTTTTCCCATGTTTGCGGGTTGTTTGACTGGTGGTGTTTTGTTTAGTTACATCAGTTCTGCTTCT
GCGGTATTGATGGATCAATACGGTTTATCTCAGCAGCAATTTGCCTATGCTTTTGGTTTCAATGCACTCGGGATCATTAT
TTTATCTTCACTGAACAAAAAGCTCGTTTCAAGATTAAGTACATTACAGCGCCTTAAAATTGGTGGATTTATACAATTAA
CAGGGGCATTGGTTGTTTTAGTCAGCGGTCTGCTGATACACGCGCCTATAGTGATGGTGATGACAGGTTTATTTTTGGCA
GTGTCTGGTTTGGGAATGACAGGCCCGAATGCAATGGCGTTGGCGATGTCGCAACAAGGTTCACGTGCAGGAACAGCGAG
TGCTATTATGGGCAGCATGCAGTTTGCTTGTGGCTTATTGGCTGGTGTATTACTTAATTTTCTAATTTGGAATGCGCTGT
TTAATATGGGATTGATGATGCTGCTATTTTCAGGTGTAGGTGTAATCGCAATTTTTGTTGTCGCACGTCAGCTTAAGCTA
CAAACCACTTAA

Upstream 100 bases:

>100_bases
TTTAGTATTTGAAAAACTTTCATTGTTTTATGCTGTGAAATCATACTATGGACAGTGTAAGCTTGTCTGCACGCATAATA
TTTTAAATTAAAATGTGAAC

Downstream 100 bases:

>100_bases
GTCTTTAATTGCATACAAAAAGACCTGAATGCACAGGTCTTTTTATTCAGATCGGTCGGTTTAATCTAAAAACTGGGTAA
AACTCTCGACGCTTTCACGT

Product: MFS superfamily bicyclomycin/multidrug transport protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MSESRLNRQYSTAWIMLLALLTALGPLSIDMYLPALPQMAEDFGVSTAMVANTLPAYFLGLAIGQLVYGPISDRIGRKKP
LYVGLTLYVIASASCVLADNEWSLIFARIIQALGGCVGVVIARAAIRDRLDVQGSAQAFSSMMIVMGIAPIAAPALGAFV
LEFFHWQAIFWILALIGCISLVCIHFFFIETLVPEKRLRLSLNQVFTLYIAIFKDASFRFPMFAGCLTGGVLFSYISSAS
AVLMDQYGLSQQQFAYAFGFNALGIIILSSLNKKLVSRLSTLQRLKIGGFIQLTGALVVLVSGLLIHAPIVMVMTGLFLA
VSGLGMTGPNAMALAMSQQGSRAGTASAIMGSMQFACGLLAGVLLNFLIWNALFNMGLMMLLFSGVGVIAIFVVARQLKL
QTT

Sequences:

>Translated_403_residues
MSESRLNRQYSTAWIMLLALLTALGPLSIDMYLPALPQMAEDFGVSTAMVANTLPAYFLGLAIGQLVYGPISDRIGRKKP
LYVGLTLYVIASASCVLADNEWSLIFARIIQALGGCVGVVIARAAIRDRLDVQGSAQAFSSMMIVMGIAPIAAPALGAFV
LEFFHWQAIFWILALIGCISLVCIHFFFIETLVPEKRLRLSLNQVFTLYIAIFKDASFRFPMFAGCLTGGVLFSYISSAS
AVLMDQYGLSQQQFAYAFGFNALGIIILSSLNKKLVSRLSTLQRLKIGGFIQLTGALVVLVSGLLIHAPIVMVMTGLFLA
VSGLGMTGPNAMALAMSQQGSRAGTASAIMGSMQFACGLLAGVLLNFLIWNALFNMGLMMLLFSGVGVIAIFVVARQLKL
QTT
>Mature_402_residues
SESRLNRQYSTAWIMLLALLTALGPLSIDMYLPALPQMAEDFGVSTAMVANTLPAYFLGLAIGQLVYGPISDRIGRKKPL
YVGLTLYVIASASCVLADNEWSLIFARIIQALGGCVGVVIARAAIRDRLDVQGSAQAFSSMMIVMGIAPIAAPALGAFVL
EFFHWQAIFWILALIGCISLVCIHFFFIETLVPEKRLRLSLNQVFTLYIAIFKDASFRFPMFAGCLTGGVLFSYISSASA
VLMDQYGLSQQQFAYAFGFNALGIIILSSLNKKLVSRLSTLQRLKIGGFIQLTGALVVLVSGLLIHAPIVMVMTGLFLAV
SGLGMTGPNAMALAMSQQGSRAGTASAIMGSMQFACGLLAGVLLNFLIWNALFNMGLMMLLFSGVGVIAIFVVARQLKLQ
TT

Specific function: Involved In Sulfonamide (Sulfathiazole) And Bicyclomycin Resistance. Probable Membrane Translocase. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=372, Percent_Identity=33.6021505376344, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI48994889, Length=357, Percent_Identity=26.0504201680672, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1790146, Length=348, Percent_Identity=27.8735632183908, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1790794, Length=326, Percent_Identity=24.2331288343558, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1787065, Length=363, Percent_Identity=24.5179063360882, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87082312, Length=362, Percent_Identity=28.4530386740331, Blast_Score=86, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6324264, Length=199, Percent_Identity=30.6532663316583, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43321; Mature: 43189

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESRLNRQYSTAWIMLLALLTALGPLSIDMYLPALPQMAEDFGVSTAMVANTLPAYFLG
CCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHH
LAIGQLVYGPISDRIGRKKPLYVGLTLYVIASASCVLADNEWSLIFARIIQALGGCVGVV
HHHHHHHHCCHHHHCCCCCCEEEHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHH
IARAAIRDRLDVQGSAQAFSSMMIVMGIAPIAAPALGAFVLEFFHWQAIFWILALIGCIS
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVCIHFFFIETLVPEKRLRLSLNQVFTLYIAIFKDASFRFPMFAGCLTGGVLFSYISSAS
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
AVLMDQYGLSQQQFAYAFGFNALGIIILSSLNKKLVSRLSTLQRLKIGGFIQLTGALVVL
HHHHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
VSGLLIHAPIVMVMTGLFLAVSGLGMTGPNAMALAMSQQGSRAGTASAIMGSMQFACGLL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEEECCCCCCCCHHHHHHHHHHHHHHHH
AGVLLNFLIWNALFNMGLMMLLFSGVGVIAIFVVARQLKLQTT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SESRLNRQYSTAWIMLLALLTALGPLSIDMYLPALPQMAEDFGVSTAMVANTLPAYFLG
CCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHH
LAIGQLVYGPISDRIGRKKPLYVGLTLYVIASASCVLADNEWSLIFARIIQALGGCVGVV
HHHHHHHHCCHHHHCCCCCCEEEHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHH
IARAAIRDRLDVQGSAQAFSSMMIVMGIAPIAAPALGAFVLEFFHWQAIFWILALIGCIS
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVCIHFFFIETLVPEKRLRLSLNQVFTLYIAIFKDASFRFPMFAGCLTGGVLFSYISSAS
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
AVLMDQYGLSQQQFAYAFGFNALGIIILSSLNKKLVSRLSTLQRLKIGGFIQLTGALVVL
HHHHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
VSGLLIHAPIVMVMTGLFLAVSGLGMTGPNAMALAMSQQGSRAGTASAIMGSMQFACGLL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEEECCCCCCCCHHHHHHHHHHHHHHHH
AGVLLNFLIWNALFNMGLMMLLFSGVGVIAIFVVARQLKLQTT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 9202461 [H]