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The map label for this gene is nuoB

Identifier: 50083950

GI number: 50083950

Start: 714832

End: 715509

Strand: Direct

Name: nuoB

Synonym: ACIAD0731

Alternate gene names: 50083950

Gene position: 714832-715509 (Clockwise)

Preceding gene: 50083949

Following gene: 50083951

Centisome position: 19.86

GC content: 42.04

Gene sequence:

>678_bases
ATGAAATATACATTAACCCGTGCGAATCCGGATGCTAATCAATATCCATTACAAGAACGCCAAGTTGTGAACGATCCACT
TCAGGAAGAAGTCAATAAAAATGTATTTATGACTCGCCTTGAAGATGTATTGCATAACGTAGTGAACTGGGGCCGAAAAA
ATTCGGTTTGGCCTTTTAACTTTGGTACTTCTTGCTGTTACGTAGAATATGCAACAACCTTGACGGGTGTGCATGATTTA
TCACGTTTTGGAGCTGAGGTAATTCGTGCTTCACCTCGTCAGGCTGACCTGATGATTGTTGCAGGAACGTGCTTTGTCAA
AATGGCACCTGTTATCCAGCGTTTGTACGAACAAATGCTTGAGCCGAAGTGGGTTATTTCCATGGGTGCATGTGCAAACT
CAGGTGGTATGTACGATATTTATTCTGTCGTACAGGGTGTTGATAAAATCATTCCTGTTGATGTGTATGTTCCAGGATGT
CCTCCTCGTCCAGAAGCACTCATTCAGGCACTTATGCTCTTGCAAGATCAAATTCAGCTTGAGCGTCGTCCATTATCAGC
CGTCATTGGTGATGATCTTCAGCCAGTATATAAGCCAAAGATGATGCCAGAACGTGACCGAAAAAATGCTGAACGGATTG
CAGTGAAAAACTTACGATCTATGGATGAAATTAAATAA

Upstream 100 bases:

>100_bases
GTTGGTCATCGATCCAACTGGTCATATCCCTGCACAATCCTCTGGTCGAATGAAATCCAAAACATCGACAGCACCGTCAT
CTAAACAGGAGTAACTCGGG

Downstream 100 bases:

>100_bases
TTTTTTTGATTTTCCATCTCTATGTTGAGCATGGTTGTCAAAAACCAAAAAAGAATTTGTATTAAATAGGAAGCCAAACC
AATGGCTGAAACTGACATTG

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MKYTLTRANPDANQYPLQERQVVNDPLQEEVNKNVFMTRLEDVLHNVVNWGRKNSVWPFNFGTSCCYVEYATTLTGVHDL
SRFGAEVIRASPRQADLMIVAGTCFVKMAPVIQRLYEQMLEPKWVISMGACANSGGMYDIYSVVQGVDKIIPVDVYVPGC
PPRPEALIQALMLLQDQIQLERRPLSAVIGDDLQPVYKPKMMPERDRKNAERIAVKNLRSMDEIK

Sequences:

>Translated_225_residues
MKYTLTRANPDANQYPLQERQVVNDPLQEEVNKNVFMTRLEDVLHNVVNWGRKNSVWPFNFGTSCCYVEYATTLTGVHDL
SRFGAEVIRASPRQADLMIVAGTCFVKMAPVIQRLYEQMLEPKWVISMGACANSGGMYDIYSVVQGVDKIIPVDVYVPGC
PPRPEALIQALMLLQDQIQLERRPLSAVIGDDLQPVYKPKMMPERDRKNAERIAVKNLRSMDEIK
>Mature_225_residues
MKYTLTRANPDANQYPLQERQVVNDPLQEEVNKNVFMTRLEDVLHNVVNWGRKNSVWPFNFGTSCCYVEYATTLTGVHDL
SRFGAEVIRASPRQADLMIVAGTCFVKMAPVIQRLYEQMLEPKWVISMGACANSGGMYDIYSVVQGVDKIIPVDVYVPGC
PPRPEALIQALMLLQDQIQLERRPLSAVIGDDLQPVYKPKMMPERDRKNAERIAVKNLRSMDEIK

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=141, Percent_Identity=53.9007092198582, Blast_Score=167, Evalue=1e-41,
Organism=Escherichia coli, GI1788624, Length=226, Percent_Identity=72.5663716814159, Blast_Score=343, Evalue=5e-96,
Organism=Escherichia coli, GI1789074, Length=146, Percent_Identity=40.4109589041096, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1788834, Length=161, Percent_Identity=37.2670807453416, Blast_Score=95, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17509685, Length=147, Percent_Identity=52.3809523809524, Blast_Score=160, Evalue=7e-40,
Organism=Drosophila melanogaster, GI18859983, Length=144, Percent_Identity=54.8611111111111, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24642371, Length=144, Percent_Identity=54.8611111111111, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24651058, Length=146, Percent_Identity=54.1095890410959, Blast_Score=165, Evalue=3e-41,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB_ACIAD (Q6FE70)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_045460.1
- ProteinModelPortal:   Q6FE70
- STRING:   Q6FE70
- GeneID:   2879581
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD0731
- NMPDR:   fig|62977.3.peg.474
- eggNOG:   COG0377
- HOGENOM:   HBG553221
- OMA:   PRPEAYI
- PhylomeDB:   Q6FE70
- ProtClustDB:   PRK06411
- BioCyc:   ASP62977:ACIAD0731-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 25591; Mature: 25591

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYTLTRANPDANQYPLQERQVVNDPLQEEVNKNVFMTRLEDVLHNVVNWGRKNSVWPFN
CCEEEECCCCCCCCCCCHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
FGTSCCYVEYATTLTGVHDLSRFGAEVIRASPRQADLMIVAGTCFVKMAPVIQRLYEQML
CCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHHC
EPKWVISMGACANSGGMYDIYSVVQGVDKIIPVDVYVPGCPPRPEALIQALMLLQDQIQL
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
ERRPLSAVIGDDLQPVYKPKMMPERDRKNAERIAVKNLRSMDEIK
HHCCHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKYTLTRANPDANQYPLQERQVVNDPLQEEVNKNVFMTRLEDVLHNVVNWGRKNSVWPFN
CCEEEECCCCCCCCCCCHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
FGTSCCYVEYATTLTGVHDLSRFGAEVIRASPRQADLMIVAGTCFVKMAPVIQRLYEQML
CCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHHC
EPKWVISMGACANSGGMYDIYSVVQGVDKIIPVDVYVPGCPPRPEALIQALMLLQDQIQL
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
ERRPLSAVIGDDLQPVYKPKMMPERDRKNAERIAVKNLRSMDEIK
HHCCHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA